LOCUS       NM_020442               3575 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens valyl-tRNA synthetase 2, mitochondrial (VARS2),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_020442 XM_373419
VERSION     NM_020442.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3575)
  AUTHORS   Chin,H.L., Goh,D.L., Wang,F.S., Tay,S.K.H., Heng,C.K., Donnini,C.,
            Baruffini,E. and Pines,O.
  TITLE     A combination of two novel VARS2 variants causes a mitochondrial
            disorder associated with failure to thrive and pulmonary
            hypertension
  JOURNAL   J Mol Med (Berl) 97 (11), 1557-1566 (2019)
   PUBMED   31529142
  REMARK    GeneRIF: Study shows A combination of two novel VARS2 variants
            causes a mitochondrial disorder associated with failure to thrive
            and pulmonary hypertension
REFERENCE   4  (bases 1 to 3575)
  AUTHORS   Begliuomini,C., Magli,G., Di Rocco,M., Santorelli,F.M.,
            Cassandrini,D., Nesti,C., Deodato,F., Diodato,D., Casellato,S.,
            Simula,D.M., Dessi,V., Eusebi,A., Carta,A. and Sotgiu,S.
  TITLE     VARS2-linked mitochondrial encephalopathy: two case reports
            enlarging the clinical phenotype
  JOURNAL   BMC Med Genet 20 (1), 77 (2019)
   PUBMED   31064326
  REMARK    GeneRIF: report two additional cases of two non-related young girls
            from Sardinia, born from non-consanguineous and healthy parents,
            carrying the aforesaid homozygous VARS2 variant. At onset both the
            patients presented with worsening psychomotor delay, muscle
            hypotonia and brisk tendon reflexes
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3575)
  AUTHORS   Diodato,D., Melchionda,L., Haack,T.B., Dallabona,C., Baruffini,E.,
            Donnini,C., Granata,T., Ragona,F., Balestri,P., Margollicci,M.,
            Lamantea,E., Nasca,A., Powell,C.A., Minczuk,M., Strom,T.M.,
            Meitinger,T., Prokisch,H., Lamperti,C., Zeviani,M. and Ghezzi,D.
  TITLE     VARS2 and TARS2 mutations in patients with mitochondrial
            encephalomyopathies
  JOURNAL   Hum Mutat 35 (8), 983-989 (2014)
   PUBMED   24827421
REFERENCE   6  (bases 1 to 3575)
  AUTHORS   Chae,Y.S., Lee,S.J., Moon,J.H., Kang,B.W., Kim,J.G., Sohn,S.K.,
            Jung,J.H., Park,H.Y., Park,J.Y., Kim,H.J. and Lee,S.W.
  TITLE     VARS2 V552V variant as prognostic marker in patients with early
            breast cancer
  JOURNAL   Med Oncol 28 (4), 1273-1280 (2011)
   PUBMED   20503108
  REMARK    GeneRIF: VARS2 V552V may be considered as a prognostic factor for
            survival in patients with early breast cancer.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 3575)
  AUTHORS   Cagliani,R., Riva,S., Pozzoli,U., Fumagalli,M., Comi,G.P.,
            Bresolin,N., Clerici,M. and Sironi,M.
  TITLE     Balancing selection is common in the extended MHC region but most
            alleles with opposite risk profile for autoimmune diseases are
            neutrally evolving
  JOURNAL   BMC Evol Biol 11, 171 (2011)
   PUBMED   21682861
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3575)
  AUTHORS   Vignal,C., Bansal,A.T., Balding,D.J., Binks,M.H., Dickson,M.C.,
            Montgomery,D.S. and Wilson,A.G.
  TITLE     Genetic association of the major histocompatibility complex with
            rheumatoid arthritis implicates two non-DRB1 loci
  JOURNAL   Arthritis Rheum 60 (1), 53-62 (2009)
   PUBMED   19116923
REFERENCE   9  (bases 1 to 3575)
  AUTHORS   Rorbach,J., Yusoff,A.A., Tuppen,H., Abg-Kamaludin,D.P.,
            Chrzanowska-Lightowlers,Z.M., Taylor,R.W., Turnbull,D.M.,
            McFarland,R. and Lightowlers,R.N.
  TITLE     Overexpression of human mitochondrial valyl tRNA synthetase can
            partially restore levels of cognate mt-tRNAVal carrying the
            pathogenic C25U mutation
  JOURNAL   Nucleic Acids Res 36 (9), 3065-3074 (2008)
   PUBMED   18400783
  REMARK    GeneRIF: Data indicate that variations in the levels of VARS2L
            between tissue types and patients could underlie the difference in
            clinical presentation between individuals homoplasmic for the
            1624C>T MTTV (equivalent to mt-tRNAVal C25U) mutation.
REFERENCE   10 (bases 1 to 3575)
  AUTHORS   Bonnefond,L., Fender,A., Rudinger-Thirion,J., Giege,R., Florentz,C.
            and Sissler,M.
  TITLE     Toward the full set of human mitochondrial aminoacyl-tRNA
            synthetases: characterization of AspRS and TyrRS
  JOURNAL   Biochemistry 44 (12), 4805-4816 (2005)
   PUBMED   15779907
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK303415.1, AK294451.1 and
            BC073838.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 25, 2020 this sequence version replaced NM_020442.5.
            
            Summary: This gene encodes a mitochondrial aminoacyl-tRNA
            synthetase, which catalyzes the attachment of valine to tRNA(Val)
            for mitochondrial translation. Mutations in this gene cause
            combined oxidative phosphorylation deficiency-20, and are also
            associated with early-onset mitochondrial encephalopathies.
            Alternative splicing of this gene results in multiple transcript
            variants. [provided by RefSeq, Aug 2014].
            
            Transcript Variant: This variant (2) contains alternate 5' exon
            structure, and it thus differs in the 5' UTR, lacks a portion of
            the 5' coding region, and initiates translation at a downstream
            in-frame start codon, compared to variant 1. The encoded isoform
            (2) is shorter at the N-terminus, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK303415.1, SRR14038195.2290522.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000676266.1/
                                                  ENSP00000502585.1
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-154               AK303415.1         20-173
            155-302             AK294451.1         129-276
            303-1421            AK303415.1         322-1440
            1422-3575           BC073838.1         70-2223
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.33"
     gene            1..3575
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /note="valyl-tRNA synthetase 2, mitochondrial"
                     /db_xref="GeneID:57176"
                     /db_xref="HGNC:HGNC:21642"
                     /db_xref="MIM:612802"
     exon            1..107
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            108..335
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..3326
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /EC_number="6.1.1.9"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; valyl-tRNA synthetase like; valine tRNA ligase
                     2, mitochondrial (putative)"
                     /codon_start=1
                     /product="valine--tRNA ligase, mitochondrial isoform 2
                     precursor"
                     /protein_id="NP_065175.4"
                     /db_xref="CCDS:CCDS34387.1"
                     /db_xref="GeneID:57176"
                     /db_xref="HGNC:HGNC:21642"
                     /db_xref="MIM:612802"
                     /translation="MPHLPLASFRPPFWGLRHSRGLPRFHSVSTQSEPHGSPISRRNR
                     EAKQKRLREKQATLEAEIAGESKSPAESIKAWRPKELVLYEIPTKPGEKKDVSGPLPP
                     AYSPRYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTV
                     AIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREV
                     WQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQL
                     VNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVV
                     GTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVK
                     VTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSEWG
                     LFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQ
                     KNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAEL
                     TGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLF
                     WVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQ
                     EKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQ
                     SCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFL
                     TRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPL
                     MPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLR
                     ALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWA
                     QAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQK
                     LSSLQLELSKLDKAASHLRQLMDEPPAPGSPEL"
     exon            336..417
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            418..518
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            519..640
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            641..707
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            708..805
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            806..887
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1007
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1119
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1208
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1299
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1427
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1531
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1613
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1690
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1766
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..1869
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1870..1940
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            1941..2066
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2171
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            2172..2240
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            2241..2319
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            2320..2447
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            2448..2600
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2807
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2919
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            2920..3095
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            3096..3224
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     exon            3225..3575
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3554..3559
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /note="hexamer: GATAAA"
     polyA_site      3575
                     /gene="VARS2"
                     /gene_synonym="COXPD20; VALRS; VARS2L; VARSL"
                     /note="major polyA site"
ORIGIN      
        1 gcccccatgc gccgcgcggc tccagggcca cgttccaggg tcgggtttgg tggattcctc
       61 agtccctgcc gccgcggggc gccctgggat agcggcgggg cctcctgatc tcggcccctt
      121 tccaaacact cctgatgcct catttgcctc tcgcctcttt tcgaccacca ttttgggggc
      181 tgaggcactc acggggcctc cccaggtttc actccgtttc tacacagtcg gagccccatg
      241 gatctcccat ctcccggagg aaccgtgaag ccaaacagaa gcgcctgcga gagaagcagg
      301 cgactctgga ggctgagata gcaggggaga gcaagtcacc tgcagaatcc attaaggcct
      361 ggaggcctaa ggagttagta ttgtatgaaa tccctacgaa acccggtgaa aagaaagatg
      421 tctctgggcc cctgcctcct gcatacagcc cccgatatgt tgaggctgcc tggtacccgt
      481 ggtgggtacg agagggcttc ttcaaaccag aatatcaggc ccggctgccc caagctacag
      541 gggagacctt ttccatgtgt atcccacctc ccaatgtcac tggctccctg cacattggcc
      601 acgcactcac ggtggccata caggatgccc tcgtgcgctg gcaccggatg cgtggggatc
      661 aagtgctgtg ggtccctggt tcagatcatg caggaattgc tacacaagct gtggtggaga
      721 aacaactgtg gaaggaacgg ggagtgagga gacatgagct gagccgggag gccttcctta
      781 gggaggtgtg gcagtggaag gaggcgaaag gtggagagat ctgtgagcag ctgcgagctc
      841 tgggtgcctc cctggactgg gatcgagagt gttttaccat ggatgttggc tcctcagtgg
      901 ctgtgactga agcttttgtg cggctctaca aggcggggtt gctgtaccgg aaccatcagc
      961 ttgtcaactg gtcatgtgct ttaagatcag ccatctcgga cattgaggtg gagaaccggc
     1021 ccctgcctgg ccacacacag cttcgactgc ctggctgccc cacccccgtg tcttttggcc
     1081 tcctattttc tgttgccttc cccgtggatg gagagcctga tgcagaggtt gtggtaggaa
     1141 ccacaaggcc agagacgctg cctggagatg tggctgtggc cgttcatcca gacgactcgc
     1201 gatacacaca tctacacggg cgacagcttc gtcacccctt gatggggcag cctcttcccc
     1261 tcatcacaga ctatgctgtt cagccacatg tgggcacggg ggcagtgaag gtgactccag
     1321 ctcacagtcc tgccgatgct gagatggggg cccgacatgg cttgagcccc ttgaatgtca
     1381 ttgcggagga tgggaccatg acctccctct gcggggactg gctgcagggt cttcaccggt
     1441 ttgtggcccg ggaaaagata atgtctgtgc tgagtgaatg gggcctgttc cggggcctcc
     1501 agaaccaccc catggtactg cccatctgca gccgttctgg ggatgtgata gaatacctgc
     1561 tgaagaacca gtggtttgtc cgctgccagg aaatgggggc ccgagctgcc aaggctgtgg
     1621 agtcgggggc cctggagctc agtccctcct tccaccagaa gaactggcag cactggtttt
     1681 cccatattgg ggactggtgt gtctcccggc agctgtggtg gggccatcag attccagcct
     1741 acctggttgt agaggaccat gcgcagggag aagaggactg ttgggtggtt gggcggtcag
     1801 aggctgaggc cagagaggta gcagcggaac tgacagggag gccaggggca gagctgaccc
     1861 tggagaggga tcctgatgtc ctagacacat ggttttcttc tgccctgttc cccttttctg
     1921 ccctgggctg gccccaagag accccagacc ttgctcgttt ctaccccctg tcacttttgg
     1981 aaacgggcag cgaccttctg ctgttctggg tgggccgcat ggtcatgttg gggacccagc
     2041 tcacagggca gctgcccttc agcaaggtgc ttcttcatcc catggttcgg gacaggcagg
     2101 gccggaagat gagcaagtcc ctggggaatg tgctggaccc aagagacatc atcagtgggg
     2161 tggagatgca ggtgctgcag gaaaagctga gaagcggaaa tttggaccct gcagagctgg
     2221 ccattgtggc tgcagcacag aaaaaggact ttcctcacgg gatccctgag tgtgggacag
     2281 atgccctgag attcacactc tgctcccatg gagttcaggc gggcgacttg cacctgtcag
     2341 tctctgaggt ccagagctgc cgacatttct gcaacaagat ctggaatgct cttcgcttta
     2401 tcctcaatgc tttaggggag aaatttgtgc cacagcctgc tgaggagctg tctccctcct
     2461 ccccgatgga tgcctggatc ctgagccgcc ttgccctggc tgcccaggag tgtgagcggg
     2521 gcttcctcac ccgagagctc tcgctcgtca ctcatgccct gcaccacttc tggcttcaca
     2581 acctctgtga cgtctacctg gaggctgtga agcccgtgct gtggcactcg ccccgccccc
     2641 tggggccccc tcaggtcctg ttctcctgcg ctgacctcgg cctccgcctc ctggccccac
     2701 tgatgccctt cctggctgaa gagctctggc agaggctgcc ccccaggcct ggttgccccc
     2761 ctgcccccag catctcggtt gccccctacc ccagcgcctg cagcttggag cactggcgcc
     2821 agccagagct ggagcggcgc ttctcccggg tccaagaggt cgtgcaggtg ctaagggctc
     2881 tccgagccac gtaccagctc accaaagccc ggccccgagt gctgctgcag agctcagagc
     2941 ctggggacca gggcctcttc gaggccttct tggagcccct gggcaccctg ggctactgtg
     3001 gggctgtggg cctgttaccc ccaggcgcag cagctccctc cggctgggcc caggctccac
     3061 tcagtgacac ggctcaagtc tacatggagc tgcagggcct ggtggacccg cagatccagc
     3121 tacctctgtt agccgcccga aggtacaagt tgcagaagca gcttgacagc ctcacagcca
     3181 ggaccccatc agaaggggag gcagggactc agaggcaaca aaagctttct tccctccagc
     3241 tggaattgtc aaaactggac aaggcagcct ctcacctccg gcagctgatg gatgagcctc
     3301 cagccccagg gagcccggag ctctaactca tcatccccat cagttttcct ccctctcaga
     3361 cctgtctttg aggacaaaca gatttgtcag ctgtcagggt gcagtgggac gtcagagact
     3421 atgtggtcca tcgccttcat tgtgtaaatg aggacacaga ctggcttggt cgcagtgact
     3481 gtggtgtcct tgagatgctc acattactgc ccggcctgcc tcccacctgg aagtctggga
     3541 atgaggagat tgagataaac ttttgaaatc ccaaa
//
