LOCUS       NM_006295               4111 bp    mRNA    linear   PRI 19-AUG-2024
DEFINITION  Homo sapiens valyl-tRNA synthetase 1 (VARS1), mRNA.
ACCESSION   NM_006295 XM_936161 XM_936204
VERSION     NM_006295.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   El-Hachem,N., Leclercq,M., Susaeta Ruiz,M., Vanleyssem,R.,
            Shostak,K., Korner,P.R., Capron,C., Martin-Morales,L.,
            Roncarati,P., Lavergne,A., Blomme,A., Turchetto,S., Goffin,E.,
            Thandapani,P., Tarassov,I., Nguyen,L., Pirotte,B., Chariot,A.,
            Marine,J.C., Herfs,M., Rapino,F., Agami,R. and Close,P.
  TITLE     Valine aminoacyl-tRNA synthetase promotes therapy resistance in
            melanoma
  JOURNAL   Nat Cell Biol 26 (7), 1154-1164 (2024)
   PUBMED   38849541
  REMARK    GeneRIF: Valine aminoacyl-tRNA synthetase promotes therapy
            resistance in melanoma.
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Shi,R., DU,W., He,Y., Hu,J., Yu,H., Zhou,W., Guo,J. and Feng,X.
  TITLE     High expression of VARS promotes the growth of multiple myeloma
            cells by causing imbalance in valine metabolism
  JOURNAL   Zhong Nan Da Xue Xue Bao Yi Xue Ban 48 (6), 795-808 (2023)
   PUBMED   37587064
  REMARK    GeneRIF: High expression of VARS promotes the growth of multiple
            myeloma cells by causing imbalance in valine metabolism.
REFERENCE   3  (bases 1 to 4111)
  AUTHORS   Hu,W., Liu,Z., Yu,W., Wen,S., Wang,X., Qi,X., Hao,H., Lu,Y., Li,J.,
            Li,S. and Zhou,H.
  TITLE     Effects of PPM1K rs1440581 and rs7678928 on serum branched-chain
            amino acid levels and risk of cardiovascular disease
  JOURNAL   Ann Med 53 (1), 1316-1326 (2021)
   PUBMED   34382495
  REMARK    GeneRIF: Effects of PPM1K rs1440581 and rs7678928 on serum
            branched-chain amino acid levels and risk of cardiovascular
            disease.
REFERENCE   4  (bases 1 to 4111)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 4111)
  AUTHORS   Friedman,J., Smith,D.E., Issa,M.Y., Stanley,V., Wang,R.,
            Mendes,M.I., Wright,M.S., Wigby,K., Hildreth,A., Crawford,J.R.,
            Koehler,A.E., Chowdhury,S., Nahas,S., Zhai,L., Xu,Z., Lo,W.S.,
            James,K.N., Musaev,D., Accogli,A., Guerrero,K., Tran,L.T.,
            Omar,T.E.I., Ben-Omran,T., Dimmock,D., Kingsmore,S.F.,
            Salomons,G.S., Zaki,M.S., Bernard,G. and Gleeson,J.G.
  TITLE     Biallelic mutations in valyl-tRNA synthetase gene VARS are
            associated with a progressive neurodevelopmental epileptic
            encephalopathy
  JOURNAL   Nat Commun 10 (1), 707 (2019)
   PUBMED   30755602
  REMARK    GeneRIF: Variants map to the VARS tRNA binding domain and adjacent
            to the anticodon domain, and disrupt highly conserved residues.
            Patient primary cells show intact VARS protein but reduced
            enzymatic activity, suggesting partial loss of function. The
            implication of VARS in pediatric neurodegeneration broadens the
            spectrum of human diseases due to mutations in tRNA synthetase
            genes.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4111)
  AUTHORS   Bec,G., Kerjan,P. and Waller,J.P.
  TITLE     Reconstitution in vitro of the valyl-tRNA synthetase-elongation
            factor (EF) 1 beta gamma delta complex. Essential roles of the
            NH2-terminal extension of valyl-tRNA synthetase and of the EF-1
            delta subunit in complex formation
  JOURNAL   J Biol Chem 269 (3), 2086-2092 (1994)
   PUBMED   8294461
REFERENCE   7  (bases 1 to 4111)
  AUTHORS   Hsieh,S.L. and Campbell,R.D.
  TITLE     Evidence that gene G7a in the human major histocompatibility
            complex encodes valyl-tRNA synthetase
  JOURNAL   Biochem J 278 (Pt 3) (Pt 3), 809-816 (1991)
   PUBMED   1898367
  REMARK    Erratum:[Biochem J 1992 Feb 1;281(Pt 3):879]
REFERENCE   8  (bases 1 to 4111)
  AUTHORS   Bec,G., Kerjan,P., Zha,X.D. and Waller,J.P.
  TITLE     Valyl-tRNA synthetase from rabbit liver. I. Purification as a
            heterotypic complex in association with elongation factor 1
  JOURNAL   J Biol Chem 264 (35), 21131-21137 (1989)
   PUBMED   2556394
REFERENCE   9  (bases 1 to 4111)
  AUTHORS   Sargent,C.A., Dunham,I. and Campbell,R.D.
  TITLE     Identification of multiple HTF-island associated genes in the human
            major histocompatibility complex class III region
  JOURNAL   EMBO J 8 (8), 2305-2312 (1989)
   PUBMED   2477242
REFERENCE   10 (bases 1 to 4111)
  AUTHORS   Motorin,Yu.A., Wolfson,A.D., Orlovsky,A.F. and Gladilin,K.L.
  TITLE     Mammalian valyl-tRNA synthetase forms a complex with the first
            elongation factor
  JOURNAL   FEBS Lett 238 (2), 262-264 (1988)
   PUBMED   3169261
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CX781162.1, CX167000.1,
            BC012808.2 and M98326.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_006295.2.
            
            Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of
            tRNA by their cognate amino acid. Because of their central role in
            linking amino acids with nucleotide triplets contained in tRNAs,
            aminoacyl-tRNA synthetases are thought to be among the first
            proteins that appeared in evolution. The protein encoded by this
            gene belongs to class-I aminoacyl-tRNA synthetase family and is
            located in the class III region of the major histocompatibility
            complex. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X59303.1, BC012808.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000375663.8/
                                                  ENSP00000364815.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-390               CX781162.1         202-591
            391-820             CX167000.1         90-519
            821-1574            BC012808.2         774-1527
            1575-2651           M98326.1           735-1811
            2652-4111           BC012808.2         2605-4064
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.33"
     gene            1..4111
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /note="valyl-tRNA synthetase 1"
                     /db_xref="GeneID:7407"
                     /db_xref="HGNC:HGNC:12651"
                     /db_xref="MIM:192150"
     exon            1..207
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            208..627
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..4035
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /EC_number="6.1.1.9"
                     /note="valyl-tRNA synthetase 2; valine tRNA ligase 1,
                     cytoplasmic; valRS; protein G7a"
                     /codon_start=1
                     /product="valine--tRNA ligase"
                     /protein_id="NP_006286.1"
                     /db_xref="CCDS:CCDS34412.1"
                     /db_xref="GeneID:7407"
                     /db_xref="HGNC:HGNC:12651"
                     /db_xref="MIM:192150"
                     /translation="MSTLYVSPHPDAFPSLRALIAARYGEAGEGPGWGGAHPRICLQP
                     PPTSRTPFPPPRLPALEQGPGGLWVWGATAVAQLLWPAGLGGPGGSRAAVLVQQWVSY
                     ADTELIPAACGATLPALGLRSSAQDPQAVLGALGRALSPLEEWLRLHTYLAGEAPTLA
                     DLAAVTALLLPFRYVLDPPARRIWNNVTRWFVTCVRQPEFRAVLGEVVLYSGARPLSH
                     QPGPEAPALPKTAAQLKKEAKKREKLEKFQQKQKIQQQQPPPGEKKPKPEKREKRDPG
                     VITYDLPTPPGEKKDVSGPMPDSYSPRYVEAAWYPWWEQQGFFKPEYGRPNVSAANPR
                     GVFMMCIPPPNVTGSLHLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVV
                     EKKLWREQGLSRHQLGREAFLQEVWKWKEEKGDRIYHQLKKLGSSLDWDRACFTMDPK
                     LSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEVDKKELTGRTLLSVPGYKE
                     KVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDTRYQHLKGKNVIH
                     PFLSRSLPIVFDEFVDMDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINV
                     PPPFLGLPRFEARKAVLVALKERGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRC
                     GEMAQAASAAVTRGDLRILPEAHQRTWHAWMDNIREWCISRQLWWGHRIPAYFVTVSD
                     PAVPPGEDPDGRYWVSGRNEAEAREKAAKEFGVSPDKISLQQDEDVLDTWFSSGLFPL
                     SILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKLTGRLPFREVYLHAIVR
                     DAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSEVEKAKEGQKADFPAGI
                     PECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSP
                     TSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECL
                     KPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCV
                     TPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGAL
                     ASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGLVDPARELGKL
                     QAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQK
                     ML"
     exon            628..762
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            763..901
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1026
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1111
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1212
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1340
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1505
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1587
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1707
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..1816
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1911
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2002
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2127
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2231
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2390
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2391..2481
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2482..2587
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2588..2658
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2659..2784
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2785..2889
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2890..2958
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            2959..3037
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3038..3165
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3166..3321
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3322..3528
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3529..3640
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3641..3966
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     exon            3967..4111
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4090..4095
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /note="hexamer: AATAAA"
     polyA_site      4111
                     /gene="VARS1"
                     /gene_synonym="G7A; NDMSCA; VARS; VARS2"
                     /note="major polyA site"
ORIGIN      
        1 aggcccggaa acgctccctg tcacaaaggg gggaacacgt gggcgccggc tgccggggcg
       61 gcgatcttag ggaactaggg tcacctggag agccgcccac cgtctctgcc cgctcgactc
      121 ctccgcccgg gccgctcggc cggtccagcc gcggccggcg cctggctgtg aggtggattc
      181 ccggcccagt ctgaccatct ccctccagtt tttccacttc gttcggacct tctcataact
      241 atgtccaccc tctacgtctc ccctcaccca gatgccttcc ccagcctccg agccctcata
      301 gccgctcgct atggggaggc tggggagggt cccggatggg gaggagccca cccccgcatc
      361 tgtctccagc cacccccgac tagcaggact ccctttcccc caccccgcct gccggccctg
      421 gagcaggggc ccggtgggct ctgggtgtgg ggggccacgg ctgtggccca gctgctgtgg
      481 ccagcaggcc tggggggccc agggggcagc cgggcggctg tccttgtcca acagtgggtc
      541 agttacgccg acacggagtt aataccagct gcctgtggag caacgctgcc ggccctggga
      601 ctccgaagct cggcccagga cccccaggct gtgctggggg ccctgggcag ggccctgagc
      661 cccttggagg agtggcttcg gctgcacacc tacttggccg gggaggcccc cactctggct
      721 gacctggcgg ctgtcacagc cttgctgctg cctttccgat acgtcctaga cccacctgcc
      781 cgccggatct ggaataatgt gactcgctgg tttgtcacgt gtgtccggca gccagaattc
      841 cgagccgtgc taggagaagt ggttctatac tcaggagcca ggcctctctc tcatcagcca
      901 ggccccgagg ctcctgccct cccaaagaca gctgctcagc tcaagaaaga ggcaaagaaa
      961 cgggagaagc tagagaaatt ccaacagaag cagaagatcc aacagcagca gccacctcca
     1021 ggggagaaga aaccaaaacc agagaagagg gagaaacggg atcctggggt cattacctat
     1081 gacctcccaa ccccacccgg ggaaaagaaa gatgtcagtg gccccatgcc cgactcctac
     1141 agccctcggt atgtggaggc tgcctggtac ccttggtggg agcagcaggg cttcttcaag
     1201 ccagagtatg ggcgtcctaa tgtgtcagca gcaaatcccc gaggtgtctt catgatgtgc
     1261 atcccacccc ccaatgtgac aggctccctg cacctgggcc atgcactcac caacgccatc
     1321 caggactccc tgactcgatg gcaccgcatg cgtggggaga ccaccctgtg gaaccctggc
     1381 tgtgaccatg caggtattgc cacccaggtg gtggtggaga agaagctatg gcgtgagcag
     1441 ggactgagcc ggcaccagct gggccgcgag gcctttctac aggaagtctg gaagtggaag
     1501 gaggagaaag gtgaccggat ttaccaccag ttgaagaagc ttggcagctc cttggactgg
     1561 gatcgagcct gtttcaccat ggaccctaaa ctctcagcag ctgtgacaga ggcctttgtc
     1621 cggcttcacg aggaaggcat catctatcgc agtacccgcc ttgttaactg gtcctgcacc
     1681 ctcaactccg ccatctctga cattgaggtg gataagaagg agctgacagg tcgcaccctg
     1741 ctctccgtgc ctggctacaa ggagaaggtg gagttcgggg tcctcgtgtc ctttgcctat
     1801 aaggtccaag gctcagatag cgacgaggag gtggtggtgg caacaactcg gatcgagaca
     1861 atgctgggag atgtggctgt agctgtgcac cccaaagata ccagatacca gcacctgaag
     1921 gggaagaacg tgatccaccc attcctgtct cggagccttc ccattgtctt cgatgaattt
     1981 gtggacatgg actttggcac aggtgctgtg aagatcaccc ccgcacatga ccaaaatgac
     2041 tatgaagttg ggcagcggca cgggctggag gccatcagca tcatggactc ccggggggcc
     2101 ctcatcaatg tgcctccgcc tttcctgggc ctgcccaggt ttgaggccag gaaagcggtg
     2161 ctggtggcgc tgaaggagcg gggactgttc cgtggcattg aggacaaccc catggtggtg
     2221 ccactttgca accggtcgaa ggacgtggta gagcctctgc tgcggccgca gtggtacgtt
     2281 cgctgcgggg agatggccca ggctgccagc gccgctgtga ctcggggtga cctccgcatc
     2341 ctgcctgagg cccatcagcg cacatggcat gcctggatgg acaacatccg ggagtggtgc
     2401 atttccaggc agctgtggtg gggccatcgc atcccagcct actttgtcac tgtcagtgac
     2461 ccagcggtgc cccctgggga ggaccctgat gggcggtact gggtgagtgg acgcaatgag
     2521 gcggaggccc gggagaaggc agccaaggag ttcggagtgt cccctgacaa gatcagtctc
     2581 cagcaagatg aggatgtatt ggatacctgg ttctcctctg gcctcttccc cttatccatt
     2641 ttgggctggc ccaaccagtc agaagacctg agtgtgttct accccgggac actgctggag
     2701 accggtcatg acatcctctt cttctgggtg gcccggatgg tcatgctggg cctgaagctc
     2761 acgggcaggc tgccctttag agaggtctac ctccatgcca tcgtgcgaga tgctcacggc
     2821 cggaagatga gcaagtctct aggcaatgtc atcgatcccc tggacgtcat ctatggaatc
     2881 tccctgcagg gcctccacaa ccagctgctg aacagcaacc tggatcccag cgaggtggag
     2941 aaggccaaag aagggcagaa agctgacttc ccagcgggga ttcctgaatg tggcaccgat
     3001 gctctccggt ttggattatg tgcctacatg tcccagggtc gtgacatcaa cctggatgtg
     3061 aaccggatac tgggttaccg ccacttctgc aacaagctct ggaatgccac caagtttgcc
     3121 cttcgtggcc ttgggaaggg ttttgtgccc tcacccacct cccagcccgg aggccatgag
     3181 agcctggtgg accgctggat ccgcagccgc ctgacagagg ctgtgaggct cagcaatcaa
     3241 ggcttccagg cctacgactt cccggccgtc accactgccc agtacagctt ctggctctat
     3301 gagctctgtg atgtctactt ggagtgcctg aaacctgtac tgaatggggt ggaccaggtg
     3361 gcagctgagt gtgcccgcca gaccctgtac acttgcctgg acgttggcct gcggctgctc
     3421 tcacccttca tgcccttcgt gacggaggag ctgttccaga ggctgccccg gaggatgccg
     3481 caagctcccc ctagcctctg tgttaccccc tacccggagc cctcagagtg ctcctggaag
     3541 gaccccgagg cagaagccgc ccttgagctg gcgctaagca tcacgcgagc cgtgcgctcc
     3601 ctgcgggccg actacaacct cacccggatc cggcctgact gtttcctgga agtggcggat
     3661 gaggccacgg gcgccctggc atcggcggtg tcgggctacg tgcaggccct ggccagcgca
     3721 ggtgtggtgg ctgttctggc cctgggggct cccgcccccc agggttgcgc tgtggctctg
     3781 gcttctgatc gctgctccat ccacctgcag cttcaggggc tggtggaccc tgcacgggag
     3841 ctgggcaagc tgcaagccaa gcgagttgag gcccagcggc aggcccagcg tctgcgggaa
     3901 cgccgtgctg cctcgggcta tcctgtcaag gtgccgctcg aagtccagga ggcagatgaa
     3961 gccaagctcc aacagacaga agcagagctc aggaaggtgg atgaggccat cgccctattc
     4021 cagaagatgc tgtgatccac cacccagctt cacccctcac ccccagcggc tcaccatggg
     4081 gatggcagca ataaaatatt ttcccacaaa a
//
