LOCUS       NM_003761                679 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens vesicle associated membrane protein 8 (VAMP8), mRNA.
ACCESSION   NM_003761
VERSION     NM_003761.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Xu,Y., Yang,T., Xu,Q., Tang,Y. and Yang,Q.
  TITLE     Vesicle-associated membrane protein 8 knockdown exerts
            anti-proliferative, pro-apoptotic, anti-autophagic, and
            pro-ferroptotic effects on colorectal cancer cells by inhibition of
            the JAK/STAT3 pathway
  JOURNAL   J Bioenerg Biomembr 56 (4), 419-431 (2024)
   PUBMED   38720136
  REMARK    GeneRIF: Vesicle-associated membrane protein 8 knockdown exerts
            anti-proliferative, pro-apoptotic, anti-autophagic, and
            pro-ferroptotic effects on colorectal cancer cells by inhibition of
            the JAK/STAT3 pathway.
REFERENCE   2  (bases 1 to 679)
  AUTHORS   Jian,F., Wang,S., Tian,R., Wang,Y., Li,C., Li,Y., Wang,S., Fang,C.,
            Ma,C. and Rong,Y.
  TITLE     The STX17-SNAP47-VAMP7/VAMP8 complex is the default SNARE complex
            mediating autophagosome-lysosome fusion
  JOURNAL   Cell Res 34 (2), 151-168 (2024)
   PUBMED   38182888
  REMARK    GeneRIF: The STX17-SNAP47-VAMP7/VAMP8 complex is the default SNARE
            complex mediating autophagosome-lysosome fusion.
REFERENCE   3  (bases 1 to 679)
  AUTHORS   Yang,S., Zhou,P., Zhang,L., Xie,X., Zhang,Y., Bo,K., Xue,J.,
            Zhang,W., Liao,F., Xu,P., Hu,Y., Yan,R., Liu,D., Chang,J. and
            Zhou,K.
  TITLE     VAMP8 suppresses the metastasis via DDX5/beta-catenin signal
            pathway in osteosarcoma
  JOURNAL   Cancer Biol Ther 24 (1), 2230641 (2023)
   PUBMED   37405957
  REMARK    GeneRIF: VAMP8 suppresses the metastasis via DDX5/beta-catenin
            signal pathway in osteosarcoma.
REFERENCE   4  (bases 1 to 679)
  AUTHORS   Ohnishi,Y., Tsuji,D. and Itoh,K.
  TITLE     Oxidative Stress Impairs Autophagy via Inhibition of Lysosomal
            Transport of VAMP8
  JOURNAL   Biol Pharm Bull 45 (11), 1609-1615 (2022)
   PUBMED   36328496
  REMARK    GeneRIF: Oxidative Stress Impairs Autophagy via Inhibition of
            Lysosomal Transport of VAMP8.
REFERENCE   5  (bases 1 to 679)
  AUTHORS   Paumet,F., Le Mao,J., Martin,S., Galli,T., David,B., Blank,U. and
            Roa,M.
  TITLE     Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast
            cells: functional role of syntaxin 4 in exocytosis and
            identification of a vesicle-associated membrane protein
            8-containing secretory compartment
  JOURNAL   J Immunol 164 (11), 5850-5857 (2000)
   PUBMED   10820264
REFERENCE   6  (bases 1 to 679)
  AUTHORS   Kim,P.K., Hollerbach,C., Trimble,W.S., Leber,B. and Andrews,D.W.
  TITLE     Identification of the endoplasmic reticulum targeting signal in
            vesicle-associated membrane proteins
  JOURNAL   J Biol Chem 274 (52), 36876-36882 (1999)
   PUBMED   10601239
REFERENCE   7  (bases 1 to 679)
  AUTHORS   Fasshauer,D., Antonin,W., Margittai,M., Pabst,S. and Jahn,R.
  TITLE     Mixed and non-cognate SNARE complexes. Characterization of assembly
            and biophysical properties
  JOURNAL   J Biol Chem 274 (22), 15440-15446 (1999)
   PUBMED   10336434
REFERENCE   8  (bases 1 to 679)
  AUTHORS   Bui,T.D., Wong,S.H., Lu,L. and Hong,W.
  TITLE     Endobrevin maps to chromosome 2 in human and chromosome 6 in mouse
  JOURNAL   Genomics 54 (3), 579-580 (1998)
   PUBMED   9878266
REFERENCE   9  (bases 1 to 679)
  AUTHORS   Wong,S.H., Zhang,T., Xu,Y., Subramaniam,V.N., Griffiths,G. and
            Hong,W.
  TITLE     Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially
            associated with the early endosome
  JOURNAL   Mol Biol Cell 9 (6), 1549-1563 (1998)
   PUBMED   9614193
REFERENCE   10 (bases 1 to 679)
  AUTHORS   Bock,J.B. and Scheller,R.H.
  TITLE     Protein transport. A fusion of new ideas
  JOURNAL   Nature 387 (6629), 133-135 (1997)
   PUBMED   9144278
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM702226.1, BC001634.1 and
            BX092149.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003761.4.
            
            Summary: This gene encodes an integral membrane protein that
            belongs to the synaptobrevin/vesicle-associated membrane protein
            subfamily of soluble N-ethylmaleimide-sensitive factor attachment
            protein receptors (SNAREs). The encoded protein is involved in the
            fusion of synaptic vesicles with the presynaptic membrane.[provided
            by RefSeq, Jun 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.194331.1,
                                           SRR1163657.202340.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263864.10/ ENSP00000263864.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 BM702226.1         96-103
            9-261               BC001634.1         1-253
            262-679             BX092149.1         248-665
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p11.2"
     gene            1..679
                     /gene="VAMP8"
                     /gene_synonym="EDB; VAMP-8"
                     /note="vesicle associated membrane protein 8"
                     /db_xref="GeneID:8673"
                     /db_xref="HGNC:HGNC:12647"
                     /db_xref="MIM:603177"
     exon            1..64
                     /gene="VAMP8"
                     /gene_synonym="EDB; VAMP-8"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..364
                     /gene="VAMP8"
                     /gene_synonym="EDB; VAMP-8"
                     /note="vesicle-associated membrane protein 8 (endobrevin)"
                     /codon_start=1
                     /product="vesicle-associated membrane protein 8"
                     /protein_id="NP_003752.2"
                     /db_xref="CCDS:CCDS1979.1"
                     /db_xref="GeneID:8673"
                     /db_xref="HGNC:HGNC:12647"
                     /db_xref="MIM:603177"
                     /translation="MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHL
                     RNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVLICVIVFIIILFIVLFATGAFS"
     exon            65..223
                     /gene="VAMP8"
                     /gene_synonym="EDB; VAMP-8"
                     /inference="alignment:Splign:2.1.0"
     exon            224..679
                     /gene="VAMP8"
                     /gene_synonym="EDB; VAMP-8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      656..661
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VAMP8"
                     /gene_synonym="EDB; VAMP-8"
                     /note="hexamer: AATAAA"
     polyA_site      679
                     /gene="VAMP8"
                     /gene_synonym="EDB; VAMP-8"
                     /note="major polyA site"
ORIGIN      
        1 accctgggag gaagccgact aggcgaattc acttactgac cggcctgggc tgctctgaga
       61 catggaggaa gccagtgaag gtggaggaaa tgatcgtgtg cggaacctgc aaagtgaggt
      121 ggagggagtt aagaatatta tgacccagaa tgtggagcgg atcctggccc ggggggaaaa
      181 cttggaacat ctccgcaaca agacagagga tctggaagcc acatctgagc acttcaagac
      241 gacatcgcag aaggtggctc gaaaattctg gtggaagaac gtgaagatga ttgtccttat
      301 ctgcgtgatt gtttttatca tcatcctctt cattgtgctc tttgccactg gtgccttctc
      361 ttaagtaaca gggaacctct cccacctgcc cttctcttca gggacaaccc tccataaatg
      421 tgtgccaaga gggtctcctt tcctgtcttc ctctacagag aatgctgctc ggtcctccta
      481 cccctcttcc cgaggccctg ctgccatgtt gtatgcccca gaaggtacct tggtcccccg
      541 gaaggagaga aaaaagagag atggactgtg gctgcatttc ttgggtcctt agagtgggct
      601 ggagagacct agagggccca gcatgtggct gggaaactgt tggtggccag tgggtaataa
      661 agacctttca gtatcccta
//
