LOCUS       NM_014232               2126 bp    mRNA    linear   PRI 03-MAY-2024
DEFINITION  Homo sapiens vesicle associated membrane protein 2 (VAMP2),
            transcript variant 1, mRNA.
ACCESSION   NM_014232
VERSION     NM_014232.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Lee,C.Y., Hubrich,D., Varga,J.K., Schafer,C., Welzel,M.,
            Schumbera,E., Djokic,M., Strom,J.M., Schonfeld,J., Geist,J.L.,
            Polat,F., Gibson,T.J., Keller Valsecchi,C.I., Kumar,M.,
            Schueler-Furman,O. and Luck,K.
  TITLE     Systematic discovery of protein interaction interfaces using
            AlphaFold and experimental validation
  JOURNAL   Mol Syst Biol 20 (2), 75-97 (2024)
   PUBMED   38225382
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Goossens,J., Cervantes Gonzalez,A., Dewit,N., Lidon,L., Fortea,J.,
            Alcolea,D., Lleo,A., Belbin,O. and Vanmechelen,E.
  TITLE     Evaluation of cerebrospinal fluid levels of synaptic vesicle
            protein, VAMP-2, across the sporadic Alzheimer's disease continuum
  JOURNAL   Alzheimers Res Ther 15 (1), 186 (2023)
   PUBMED   37898760
  REMARK    GeneRIF: Evaluation of cerebrospinal fluid levels of synaptic
            vesicle protein, VAMP-2, across the sporadic Alzheimer's disease
            continuum.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2126)
  AUTHORS   Felicio,D., Dias,A., Martins,S., Carvalho,E., Lopes,A.M., Pinto,N.,
            Lemos,C., Santos,M. and Alves-Ferreira,M.
  TITLE     Non-coding variants in VAMP2 and SNAP25 affect gene expression:
            potential implications in migraine susceptibility
  JOURNAL   J Headache Pain 24 (1), 78 (2023)
   PUBMED   37380951
  REMARK    GeneRIF: Non-coding variants in VAMP2 and SNAP25 affect gene
            expression: potential implications in migraine susceptibility.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2126)
  AUTHORS   Yoo,G., An,H.J., Yeou,S. and Lee,N.K.
  TITLE     alpha-Synuclein Disrupts Vesicle Fusion by Two Mutant-Specific
            Mechanisms
  JOURNAL   Mol Cells 45 (11), 806-819 (2022)
   PUBMED   36380732
  REMARK    GeneRIF: alpha-Synuclein Disrupts Vesicle Fusion by Two
            Mutant-Specific Mechanisms.
REFERENCE   5  (bases 1 to 2126)
  AUTHORS   Wei,X.C., Xia,Y.R., Zhou,P., Xue,X., Ding,S., Liu,L.J. and Zhu,F.
  TITLE     Hepatitis B core antigen modulates exosomal miR-135a to target
            vesicle-associated membrane protein 2 promoting chemoresistance in
            hepatocellular carcinoma
  JOURNAL   World J Gastroenterol 27 (48), 8302-8322 (2021)
   PUBMED   35068871
  REMARK    GeneRIF: Hepatitis B core antigen modulates exosomal miR-135a to
            target vesicle-associated membrane protein 2 promoting
            chemoresistance in hepatocellular carcinoma.
REFERENCE   6  (bases 1 to 2126)
  AUTHORS   Brumell,J.H., Volchuk,A., Sengelov,H., Borregaard,N., Cieutat,A.M.,
            Bainton,D.F., Grinstein,S. and Klip,A.
  TITLE     Subcellular distribution of docking/fusion proteins in neutrophils,
            secretory cells with multiple exocytic compartments
  JOURNAL   J Immunol 155 (12), 5750-5759 (1995)
   PUBMED   7499863
REFERENCE   7  (bases 1 to 2126)
  AUTHORS   Kutay,U., Ahnert-Hilger,G., Hartmann,E., Wiedenmann,B. and
            Rapoport,T.A.
  TITLE     Transport route for synaptobrevin via a novel pathway of insertion
            into the endoplasmic reticulum membrane
  JOURNAL   EMBO J 14 (2), 217-223 (1995)
   PUBMED   7835332
REFERENCE   8  (bases 1 to 2126)
  AUTHORS   Chapman,E.R., An,S., Barton,N. and Jahn,R.
  TITLE     SNAP-25, a t-SNARE which binds to both syntaxin and synaptobrevin
            via domains that may form coiled coils
  JOURNAL   J Biol Chem 269 (44), 27427-27432 (1994)
   PUBMED   7961655
REFERENCE   9  (bases 1 to 2126)
  AUTHORS   Hunt,J.M., Bommert,K., Charlton,M.P., Kistner,A., Habermann,E.,
            Augustine,G.J. and Betz,H.
  TITLE     A post-docking role for synaptobrevin in synaptic vesicle fusion
  JOURNAL   Neuron 12 (6), 1269-1279 (1994)
   PUBMED   8011337
REFERENCE   10 (bases 1 to 2126)
  AUTHORS   Archer,B.T. 3rd, Ozcelik,T., Jahn,R., Francke,U. and Sudhof,T.C.
  TITLE     Structures and chromosomal localizations of two human genes
            encoding synaptobrevins 1 and 2
  JOURNAL   J Biol Chem 265 (28), 17267-17273 (1990)
   PUBMED   1976629
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK225777.1, AK021522.1,
            AC129492.6 and BU625107.1.
            
            On Nov 23, 2018 this sequence version replaced NM_014232.2.
            
            Summary: The protein encoded by this gene is a member of the
            vesicle-associated membrane protein (VAMP)/synaptobrevin family.
            Synaptobrevins/VAMPs, syntaxins, and the 25-kD
            synaptosomal-associated protein SNAP25 are the main components of a
            protein complex involved in the docking and/or fusion of synaptic
            vesicles with the presynaptic membrane. This gene is thought to
            participate in neurotransmitter release at a step between docking
            and fusion. The protein forms a stable complex with syntaxin,
            synaptosomal-associated protein, 25 kD, and synaptotagmin. It also
            forms a distinct complex with synaptophysin. It is a likely
            candidate gene for familial infantile myasthenia (FIMG) because of
            its map location and because it encodes a synaptic vesicle protein
            of the type that has been implicated in the pathogenesis of FIMG.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.329161.1,
                                           SRR3476690.844899.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2144333
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000316509.11/ ENSP00000314214.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-509               AK225777.1         28-536
            510-869             AK021522.1         1120-1479
            870-1956            AC129492.6         154195-155281       c
            1957-2126           BU625107.1         21-190              c
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..2126
                     /gene="VAMP2"
                     /gene_synonym="NEDHAHM; SYB2; VAMP-2"
                     /note="vesicle associated membrane protein 2"
                     /db_xref="GeneID:6844"
                     /db_xref="HGNC:HGNC:12643"
                     /db_xref="MIM:185881"
     exon            1..71
                     /gene="VAMP2"
                     /gene_synonym="NEDHAHM; SYB2; VAMP-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..420
                     /gene="VAMP2"
                     /gene_synonym="NEDHAHM; SYB2; VAMP-2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     synaptobrevin 2"
                     /codon_start=1
                     /product="vesicle-associated membrane protein 2 isoform 1"
                     /protein_id="NP_055047.2"
                     /db_xref="CCDS:CCDS32561.1"
                     /db_xref="GeneID:6844"
                     /db_xref="HGNC:HGNC:12643"
                     /db_xref="MIM:185881"
                     /translation="MSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVD
                     IMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVI
                     CAIILIIIIVYFST"
     exon            72..192
                     /gene="VAMP2"
                     /gene_synonym="NEDHAHM; SYB2; VAMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            193..351
                     /gene="VAMP2"
                     /gene_synonym="NEDHAHM; SYB2; VAMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            352..403
                     /gene="VAMP2"
                     /gene_synonym="NEDHAHM; SYB2; VAMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            404..2126
                     /gene="VAMP2"
                     /gene_synonym="NEDHAHM; SYB2; VAMP-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2104..2109
                     /regulatory_class="polyA_signal_sequence"
                     /gene="VAMP2"
                     /gene_synonym="NEDHAHM; SYB2; VAMP-2"
                     /note="hexamer: AATAAA"
     polyA_site      2126
                     /gene="VAMP2"
                     /gene_synonym="NEDHAHM; SYB2; VAMP-2"
                     /note="major polyA site"
ORIGIN      
        1 agtcggagcc agcgcgagcc gccgccgcca tcactgccgc tgccaagtcc tccacccgct
       61 gcccccgcca tgtctgctac cgctgccacg gccccccctg ctgccccggc tggggagggt
      121 ggtccccctg caccccctcc aaacctcacc agtaacagga gactgcagca gacccaggcc
      181 caggtggatg aggtggtgga catcatgagg gtgaacgtgg acaaggtcct ggagcgagac
      241 cagaagctgt cggagctgga cgaccgtgca gatgcactcc aggcgggggc ctcccagttt
      301 gaaacaagcg cagccaagct caagcgcaaa tactggtgga aaaacctcaa gatgatgatc
      361 atcttgggag tgatttgcgc catcatcctc atcatcatca tagtttactt cagcacttaa
      421 atccccgagg agtctgccct gcctagagaa gggcctctcc cccaaccctc agccgttcct
      481 ccacctctca gccatatctt tcagccccca ctcccctgga tccgtgtgtg tgtgtgtccg
      541 tgtgtgtgtc cccctgtaaa tagccagctg ttatttatac atatataata ttatatatat
      601 ttggtctgtt tgtagtttta ttactagatg atttttccgg ttgtccttaa caccccttcc
      661 tgaggttccc ttcacccctc tctcttgcct tccttccctt tccctttctt cctgactagc
      721 cccaagtccc ttcatttgca tctgctatgc aatagtccct ctcctttcct tcttcttccc
      781 tcagatttag ctgatccttc ctcccaccct ggccttcctt tcctctttcc tcctcactct
      841 ccccgtcatg ctccctctgc cccgccctca aaaaaaaaaa aaaaaaaaaa aaaacaacag
      901 cacctgtcca ggcttcctta ggtacatctt ctttgtatcc attgggaggc tctgagactg
      961 gccccacttg gtcctaagaa tcccaaggtc tttgggagcg tccagcatgt taattagcgt
     1021 atcattacat actgctatcc ctttccattt ctttttgttc catcactctt ctctcaacct
     1081 gtgtttcttt ttttactgag gagttagtcc ccattagttc ttgtatcaca ttttcatttg
     1141 cacgacatta ctcgcaggtg gtggggagcc tgggcttttg gggaaccagg ctgctctggt
     1201 ccccagcatt gcctcctcct agcccctcta gtccagtttg cctcccttac cctcattttc
     1261 caaacctctt gtaccctcct ctccctcccc cagctggtat gtaagtgtct tgaagttcag
     1321 tatgttatga tggaccaata attctgccac ttcgggtttc tccctacatt cctgctcccc
     1381 agttttcatg tggggtactc aactgacatt cccatggggt ttccctccca tctgcctgat
     1441 ccacctcctc ctcccaccag gagagttggg ggttggccac aattgatctc ttgtgagagg
     1501 ggtggctacc agtgtgtgtg tgggggtcat cactgccttg gggaggagtg gggcagggca
     1561 gagaatcccc ccaattcctg cctgaaatct ctggcctcac ccctgctggg ggttggactg
     1621 aaaaccctcc tccccaattt ggggggtgtt gccccatcac tgcccagctc ctctgactgc
     1681 cccccctgaa tttagggtgg gggtactagt cactgccaat gtgtgtatgg gacttgctgg
     1741 aaaacgggga tgcttgcccc tctccaggac tattgagccc agagagagct gtcctctcat
     1801 tgggtgaact gattgaggaa gggtctattg tctttttaaa tggcacaatt ttaagggttt
     1861 gagggtacag tcccttaacc tgccacggga gggggccccc aaactttctt ccccccacac
     1921 ttctggtttt ctgtgtggag ggggagcagg gatatctaag ctgtggtgtg aaagggtagg
     1981 agagatgctg gaggtggggg tgctgtgttt tagacccccc atattatccc agtgtcccct
     2041 gcccccctct tcccccaccc catgccccca attctgtggc gcatccagat tgtgaaaatg
     2101 tacaataaat gtgtaatgag taacca
//
