LOCUS       NM_005715               4496 bp    mRNA    linear   PRI 16-FEB-2025
DEFINITION  Homo sapiens uronyl 2-sulfotransferase (UST), mRNA.
ACCESSION   NM_005715
VERSION     NM_005715.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Fang,J., Song,T., Lindahl,U. and Li,J.P.
  TITLE     Enzyme overexpression - an exercise toward understanding regulation
            of heparan sulfate biosynthesis
  JOURNAL   Sci Rep 6, 31242 (2016)
   PUBMED   27511124
  REMARK    GeneRIF: Results show that overexpression of 2OST alone in the
            HEK293 cells did not appreciably affect heparan sulfate (HS) chain
            length. Also, its simultaneous overexpression with Hsepi abolished
            the effect of Hsepi overexpression on the HS chain lengh.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Nievergelt,C.M., Maihofer,A.X., Mustapic,M., Yurgil,K.A.,
            Schork,N.J., Miller,M.W., Logue,M.W., Geyer,M.A., Risbrough,V.B.,
            O'Connor,D.T. and Baker,D.G.
  TITLE     Genomic predictors of combat stress vulnerability and resilience in
            U.S. Marines: A genome-wide association study across multiple
            ancestries implicates PRTFDC1 as a potential PTSD gene
  JOURNAL   Psychoneuroendocrinology 51, 459-471 (2015)
   PUBMED   25456346
REFERENCE   3  (bases 1 to 4496)
  AUTHORS   Sulkava,S., Ollila,H.M., Ahola,K., Partonen,T., Viitasalo,K.,
            Kettunen,J., Lappalainen,M., Kivimaki,M., Vahtera,J., Lindstrom,J.,
            Harma,M., Puttonen,S., Salomaa,V. and Paunio,T.
  TITLE     Genome-wide scan of job-related exhaustion with three replication
            studies implicate a susceptibility variant at the UST gene locus
  JOURNAL   Hum Mol Genet 22 (16), 3363-3372 (2013)
   PUBMED   23620144
  REMARK    GeneRIF: Statistical analysis showed the strongest evidence for a
            variant located in an intron of UST associated with job-related
            exhaustion.
REFERENCE   4  (bases 1 to 4496)
  AUTHORS   Uher,R., Perroud,N., Ng,M.Y., Hauser,J., Henigsberg,N., Maier,W.,
            Mors,O., Placentino,A., Rietschel,M., Souery,D., Zagar,T.,
            Czerski,P.M., Jerman,B., Larsen,E.R., Schulze,T.G., Zobel,A.,
            Cohen-Woods,S., Pirlo,K., Butler,A.W., Muglia,P., Barnes,M.R.,
            Lathrop,M., Farmer,A., Breen,G., Aitchison,K.J., Craig,I.,
            Lewis,C.M. and McGuffin,P.
  TITLE     Genome-wide pharmacogenetics of antidepressant response in the
            GENDEP project
  JOURNAL   Am J Psychiatry 167 (5), 555-564 (2010)
   PUBMED   20360315
  REMARK    GeneRIF: Clinical trial and genome-wide association study of
            gene-disease association, gene-environment interaction, and
            pharmacogenomic / toxicogenomic. (HuGE Navigator)
REFERENCE   5  (bases 1 to 4496)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 4496)
  AUTHORS   Xu,D., Song,D., Pedersen,L.C. and Liu,J.
  TITLE     Mutational study of heparan sulfate 2-O-sulfotransferase and
            chondroitin sulfate 2-O-sulfotransferase
  JOURNAL   J Biol Chem 282 (11), 8356-8367 (2007)
   PUBMED   17227754
  REMARK    GeneRIF: analysis of differences and similarities various residues
            play in the biological roles of the HS-2OST and CS-2OST enzymes
REFERENCE   7  (bases 1 to 4496)
  AUTHORS   Ohtake,S., Kimata,K. and Habuchi,O.
  TITLE     Recognition of sulfation pattern of chondroitin sulfate by uronosyl
            2-O-sulfotransferase
  JOURNAL   J Biol Chem 280 (47), 39115-39123 (2005)
   PUBMED   16192264
  REMARK    GeneRIF: 2OST transfers sulfate preferentially to the GlcA residue
            located in a unique sequence, -GalNAc(4SO(4))-GlcA-GalNAc(6SO(4))-.
REFERENCE   8  (bases 1 to 4496)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   9  (bases 1 to 4496)
  AUTHORS   Kobayashi,M., Sugumaran,G., Liu,J., Shworak,N.W., Silbert,J.E. and
            Rosenberg,R.D.
  TITLE     Molecular cloning and characterization of a human uronyl
            2-sulfotransferase that sulfates iduronyl and glucuronyl residues
            in dermatan/chondroitin sulfate
  JOURNAL   J Biol Chem 274 (15), 10474-10480 (1999)
   PUBMED   10187838
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL807246.11, AI570697.1, DB496757.1, BC093668.1, AB020316.1 and
            CA842761.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005715.2.
            
            Summary: Uronyl 2-sulfotransferase transfers sulfate to the
            2-position of uronyl residues, such as iduronyl residues in
            dermatan sulfate and glucuronyl residues in chondroitin sulfate
            (Kobayashi et al., 1999 [PubMed 10187838]).[supplied by OMIM, Mar
            2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB020316.1, SRR1803615.39716.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367463.5/ ENSP00000356433.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-105               AL807246.11        121089-121193
            106-145             AI570697.1         1-40
            146-353             DB496757.1         18-225
            354-1642            BC093668.1         1-1289
            1643-4120           AB020316.1         1345-3822
            4121-4496           CA842761.1         6-381               c
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q25.1"
     gene            1..4496
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /note="uronyl 2-sulfotransferase"
                     /db_xref="GeneID:10090"
                     /db_xref="HGNC:HGNC:17223"
                     /db_xref="MIM:610752"
     exon            1..648
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /inference="alignment:Splign:2.1.0"
     CDS             402..1622
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /note="dermatan/chondroitin sulfate 2-sulfotransferase;
                     chondroitin sulfate 2-O-sulfotransferase"
                     /codon_start=1
                     /product="uronyl 2-sulfotransferase"
                     /protein_id="NP_005706.1"
                     /db_xref="CCDS:CCDS5213.1"
                     /db_xref="GeneID:10090"
                     /db_xref="HGNC:HGNC:17223"
                     /db_xref="MIM:610752"
                     /translation="MKKKQQHPGGGADPWPHGAPMGGAPPGLGSWKRRVPLLPFLRFS
                     LRDYGFCMATLLVFCLGSLLYQLSGGPPRFLLDLRQYLGNSTYLDDHGPPPSKVLPFP
                     SQVVYNRVGKCGSRTVVLLLRILSEKHGFNLVTSDIHNKTRLTKNEQMELIKNISTAE
                     QPYLFTRHVHFLNFSRFGGDQPVYINIIRDPVNRFLSNYFFRRFGDWRGEQNHMIRTP
                     SMRQEERYLDINECILENYPECSNPRLFYIIPYFCGQHPRCREPGEWALERAKLNVNE
                     NFLLVGILEELEDVLLLLERFLPHYFKGVLSIYKDPEHRKLGNMTVTVKKTVPSPEAV
                     QILYQRMRYEYEFYHYVKEQFHLLKRKFGLKSHVSKPPLRPHFFIPTPLETEEPIDDE
                     EQDDEKWLEDIYKR"
     exon            649..692
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            693..848
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            849..928
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1082
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1180
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1338
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /inference="alignment:Splign:2.1.0"
     exon            1339..4496
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3986..3991
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /note="hexamer: ATTATA"
     polyA_site      4005
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
     regulatory      4475..4480
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /note="hexamer: AATAAA"
     polyA_site      4496
                     /gene="UST"
                     /gene_synonym="2OST; CS-2OST"
                     /note="major polyA site"
ORIGIN      
        1 agagaagcct gaaccgggac aaaacacgcc gagacctaca gacacaaagc cggggggtcc
       61 acgctggcgc tggaggcgag ccccggcggc cgcaagcgag cccgaggcgc ggcggggcgc
      121 ggggcgtggg gacgctagcg ggcgccggac gggcgcggcg ccccgtcacg ggcagcgccc
      181 cgaaccgggg ccggacacct cggccgctcg ggccgcggcg gcggggacca tgccgaagaa
      241 agtctcctga gcccggcaac ttcggcccct ccccgccccc acccggctgc cctccgcgcg
      301 gccctcccca tgtgcagccg gccagccggg ctctcctcct cgcggcggat gggtgacctt
      361 ttcctggcac gggcaggctg tgggaggcag cggagcaggc gatgaagaag aagcagcagc
      421 atcccggcgg cggcgcggat ccctggcccc atggggcccc tatggggggc gcccctccgg
      481 gcctgggcag ctggaagcgt cgggtgcccc tgctgccttt cctgcgcttc tccctccggg
      541 actacggctt ctgcatggcc accctgctgg tcttctgcct gggctccctc ctctatcagc
      601 tcagcggggg accccctcgc ttcctgctcg acctgcggca gtacttggga aattccactt
      661 acttggatga ccatggacca cctcctagta aggtactacc tttcccaagc caggtggtgt
      721 acaacagggt aggcaagtgt gggagccgta ctgtggtctt gcttctgaga atcttgtcgg
      781 agaagcacgg atttaatttg gtcacatcag acattcacaa caaaaccagg cttactaaaa
      841 atgaacaaat ggaactgatt aaaaatataa gtactgccga acaaccctat ttattcactc
      901 gacatgttca tttcctcaac ttctcaaggt ttggaggaga ccagcctgtc tacatcaaca
      961 tcattagaga ccccgtcaac cggttcttat ccaactattt tttccgtcgc tttggagact
     1021 ggagagggga acaaaatcac atgatccgca cccccagcat gaggcaggag gagcgctacc
     1081 tggatatcaa tgagtgtatt cttgaaaact atcccgagtg ctccaacccc aggttatttt
     1141 acatcattcc gtacttttgt ggacagcatc ccagatgcag ggagcctggt gaatgggccc
     1201 ttgagagagc aaagctgaac gtgaatgaaa acttcctgct cgtggggatt cttgaagagt
     1261 tggaagatgt gctgctgtta ctggaaagat ttttacctca ttacttcaag ggcgtgctca
     1321 gtatctacaa agacccagag cacaggaagc ttggaaacat gactgtgacg gtgaagaaga
     1381 ctgtcccctc tcctgaggct gtgcagatcc tctaccagcg gatgagatac gagtacgagt
     1441 tttaccacta cgtcaaagag cagttccacc tgctgaagcg caagtttgga cttaagtctc
     1501 acgtcagcaa gccccccctg aggccacact tctttatccc aactccactg gaaaccgagg
     1561 agccaatcga cgatgaagaa caggatgatg aaaagtggct ggaagatatt tataagaggt
     1621 gatgtgactg tgttgcctct atggctttat ctcccttttc cagaaagttc tttgtttggg
     1681 gaagtaaaat ccttaaggga ctaaattaat gcttgggtgc attaaaaaga acaaaacatt
     1741 cccacatgtt ggggtcattg ggagatgccc ggttttgcgg gttttatttg tttaatttta
     1801 ttctgtgttt tctcttggct ctttgggtct ttcccgggta cactagatgg ctccatccca
     1861 aggcatcttg tcataaaaca gctttccccc accccatatc atgggaaaag ggggagaaat
     1921 atagccccta gcctaataac ttatcatttg taaaatgact tataaaaata ttacctcaat
     1981 ggtaggagac atccagactt gtatatttca gtggaaatac aaaaccactt cagagaccag
     2041 ggtatctcct ctggaaggat ctaagagaag gtaagacaga ttaggacatc gaaaaggagg
     2101 atggagccag gtgccatggc ttgagcctat aatccgaggc tgaggtggga ggatcacttg
     2161 agcccaggag tttgaggttg cagtgagctg tgatcacacc actgcactcc agcctgggtg
     2221 acagagtgag actctgtctc aattaatttt ttttttttaa aggaggagga tctccatggg
     2281 taagtggttt ctacccgcat gggtagagtt ctgcctctgg tccttctcag ggggcacttt
     2341 caccaagagc agtgtaatta tctctgaaag agcaagtcag cttgtgccgc atccccaacc
     2401 aatccacagc ctggagtacc tttcaaggtc aaagtgcatg gccagctcca ttgagacatt
     2461 ccatttcaaa gcaccgtgct gacagatatc aaagtactct agcagggaaa ataatttgtt
     2521 tgctgtgtaa ggaagaatgt agacaagaca gataaatctg aaggtcatgt ggcatcaggg
     2581 aaagggcatg gctgtgtctt ttgcacccaa tatgaaacat cttctcccaa cactgcttta
     2641 atggaagttc taggaaccaa tttagctcag gcatttgact cctacagcag aagttctgag
     2701 cctgaccaca gatggtgtgt aatctatcaa acacacccct ggccaagttg ggtcctatag
     2761 gacctggtac tatgtactat tgtaacttct agttccctaa gaggtacctg ttttcagtaa
     2821 aaaggggtcc tgagttctgt gcaggtggaa gagctacccg agaactacct gagttctgtg
     2881 caggtagagt cccatttctt atgggacctg tgtgctcctg agaactctta cttgagacat
     2941 caaaaagaag cagcaagagc ttctgggaca gagactgctt ggccagcttt gtaagtaagt
     3001 ggctgcctcc aatgtgatgt gagtacatgt tgggcagtct cactgtccta aggtatgtct
     3061 tctttccacc tcccactgcc cctcccctgc cacctatcaa tgatgccttg gttcagtcat
     3121 tagaaatctg ttgctttgag ttctgaaata ttttcacctt aaaaaaaatg ctgaaaatac
     3181 acattctcct gggaagacga taaacagcta gctaagaagc cgaggttcag tggtggcagc
     3241 aggaaggaca ctgccacaaa ttttgtctat ttcatatttg tcccctagag ccagccctag
     3301 caaatgtgtg agttgggagt agttaatagt aaataagact ctgactttac acaagctaca
     3361 cattttatac ttttcataaa ccacaaagtc tctctagaat tttttctgcc ttcactaaaa
     3421 ttggactgta gccaagatat aaagcaagtc atttggaacc tgccgagtga gcactgaagc
     3481 tactttatca tgagatgtgt gttaagaagg ctgcagccca caggagtcca gggaaggcgg
     3541 ggaccacaga ggcacagagt ccagcacttg gccgctcatg ggccttcttt ctgcctcaga
     3601 ggacgggggc agagaagtga tgaagggaaa tgttcttaga ggaggaaata tcctttgtcc
     3661 tgttcagaga gaccagggcc ctaccattag gcatactttc agaagcaacc tggagaacag
     3721 ctatcaatca tattcaaaac cagtacaaga actgctgcct ggtaccctgt gagtcatttc
     3781 tatgaaattc catataaaga atgatgataa gtttacacac tgtgcaatct cacaatctga
     3841 aaataaagtt gagttggctg tgttttctct gctcttgtca gaacattggg acaattggtc
     3901 gttcaaaaac attcatcctc ttactgcaag tttatctggg tacttttacc tgtgtgttca
     3961 aaggcatttc ttttcagcag tgatcattat aacttcacaa aaaaagatgc tgacggattt
     4021 acttacaggg ccttaatgtt attttgtccc agccaacacc ctctaggtcc taaaagtcaa
     4081 ggtacttcag tttatttggc aaacatgaca acattttttt tggccctggg cccaacagtt
     4141 tgtacttcat gaaacatatt gtacatttta catagtttaa tttaaaaaat accttttaag
     4201 ctagttgatc tttgactgtc ttatttatta taacctttca gcacattcca aggttttagt
     4261 tactcaggaa ggagttaatt aaaatgattt tattttggtc tgatggatgt tttttaaaag
     4321 gaaaattatt attatgaacc ttcagcctac tttcttgagt gccgtaaaag tgcttgtaaa
     4381 tctttttttt tttttaagaa gaaagaaaaa aatggtgttt gacgttgatg gaaattcaaa
     4441 aatatatatg gaactgaaac attaacttag ctaaaataaa agcaatctgt gtttga
//
