LOCUS       NM_003470               5831 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens ubiquitin specific peptidase 7 (USP7), transcript
            variant 1, mRNA.
ACCESSION   NM_003470
VERSION     NM_003470.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Tang,K., Yin,T., Deng,B., Wang,M., Ren,Z., Wang,S., Liu,X., Li,H.,
            Wang,J., Du,Y., Zhou,J., Chen,Y. and Wang,Y.
  TITLE     USP7 deubiquitinates epigenetic reader ZMYND8 to promote breast
            cancer cell migration and invasion
  JOURNAL   J Biol Chem 300 (9), 107672 (2024)
   PUBMED   39128723
  REMARK    GeneRIF: USP7 deubiquitinates epigenetic reader ZMYND8 to promote
            breast cancer cell migration and invasion.
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Kliche,J., Simonetti,L., Krystkowiak,I., Kuss,H., Diallo,M.,
            Rask,E., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Proteome-scale characterisation of motif-based interactome rewiring
            by disease mutations
  JOURNAL   Mol Syst Biol 20 (9), 1025-1048 (2024)
   PUBMED   39009827
REFERENCE   3  (bases 1 to 5831)
  AUTHORS   Yang,Z. and Nicholas,J.
  TITLE     Biologically significant interaction of human herpesvirus 8 viral
            interferon regulatory factor 4 with ubiquitin-specific protease 7
  JOURNAL   J Virol 98 (6), e0025524 (2024)
   PUBMED   38752725
  REMARK    GeneRIF: Biologically significant interaction of human herpesvirus
            8 viral interferon regulatory factor 4 with ubiquitin-specific
            protease 7.
REFERENCE   4  (bases 1 to 5831)
  AUTHORS   Zhao,B., Xia,Z., Yang,B., Guo,Y., Zhou,R., Gu,M., Liu,M., Li,Q.,
            Bai,W., Huang,J., Zhang,X., Zhu,C., Leung,K.T., Chen,C. and Dong,J.
  TITLE     USP7 promotes IgA class switching through stabilizing RUNX3 for
            germline transcription activation
  JOURNAL   Cell Rep 43 (5), 114194 (2024)
   PUBMED   38735043
  REMARK    GeneRIF: USP7 promotes IgA class switching through stabilizing
            RUNX3 for germline transcription activation.
            Erratum:[Cell Rep. 2024 Jun 25;43(6):114315. doi:
            10.1016/j.celrep.2024.114315.. PMID: 38795349]
REFERENCE   5  (bases 1 to 5831)
  AUTHORS   Zhang,R., Zhang,D., Han,F., Song,X., Zhang,Y., Zhang,J., Zhu,Q. and
            Qin,Y.
  TITLE     The deubiquitinase USP7 and E3 ligase TRIM21 regulate vasculogenic
            mimicry and malignant progression of RMS by balancing SNAI2
            homeostasis
  JOURNAL   J Exp Clin Cancer Res 43 (1), 135 (2024)
   PUBMED   38702792
  REMARK    GeneRIF: The deubiquitinase USP7 and E3 ligase TRIM21 regulate
            vasculogenic mimicry and malignant progression of RMS by balancing
            SNAI2 homeostasis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5831)
  AUTHORS   Hao,Y.H., Fountain,M.D. Jr., Fon Tacer,K., Xia,F., Bi,W.,
            Kang,S.H., Patel,A., Rosenfeld,J.A., Le Caignec,C., Isidor,B.,
            Krantz,I.D., Noon,S.E., Pfotenhauer,J.P., Morgan,T.M., Moran,R.,
            Pedersen,R.C., Saenz,M.S., Schaaf,C.P. and Potts,P.R.
  TITLE     USP7 Acts as a Molecular Rheostat to Promote WASH-Dependent
            Endosomal Protein Recycling and Is Mutated in a Human
            Neurodevelopmental Disorder
  JOURNAL   Mol Cell 59 (6), 956-969 (2015)
   PUBMED   26365382
  REMARK    GeneRIF: The USP7 deubiquitinating enzyme is an integral component
            of the MAGE-L2-TRIM27 ligase and is essential for WASH-mediated
            endosomal actin assembly and protein recycling.
REFERENCE   7  (bases 1 to 5831)
  AUTHORS   Robinson,P.A., Lomonte,P., Leek, Markham,A.F. and Everett,R.D.
  TITLE     Assignment1 of herpesvirus-associated ubiquitin-specific protease
            gene HAUSP to human chromosome band 16p13.3 by in situ
            hybridization
  JOURNAL   Cytogenet Cell Genet 83 (1-2), 100 (1998)
   PUBMED   9925944
REFERENCE   8  (bases 1 to 5831)
  AUTHORS   D'Andrea,A. and Pellman,D.
  TITLE     Deubiquitinating enzymes: a new class of biological regulators
  JOURNAL   Crit Rev Biochem Mol Biol 33 (5), 337-352 (1998)
   PUBMED   9827704
  REMARK    Review article
REFERENCE   9  (bases 1 to 5831)
  AUTHORS   Everett,R.D., Meredith,M., Orr,A., Cross,A., Kathoria,M. and
            Parkinson,J.
  TITLE     A novel ubiquitin-specific protease is dynamically associated with
            the PML nuclear domain and binds to a herpesvirus regulatory
            protein
  JOURNAL   EMBO J 16 (7), 1519-1530 (1997)
   PUBMED   9130697
REFERENCE   10 (bases 1 to 5831)
  AUTHORS   Everett,R.D., Meredith,M., Orr,A., Cross,A., Kathoria,M. and
            Parkinson,J.
  TITLE     A novel ubiquitin-specific protease is dynamically associated with
            the PML nuclear domain and binds to a herpesvirus regulatory
            protein
  JOURNAL   EMBO J 16 (3), 566-577 (1997)
   PUBMED   9034339
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022167.7, Z72499.1 and
            AY376241.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003470.2.
            
            Summary: The protein encoded by this gene belongs to the peptidase
            C19 family, which includes ubiquitinyl hydrolases. This protein
            deubiquitinates target proteins such as p53 (a tumor suppressor
            protein) and WASH (essential for endosomal protein recycling), and
            regulates their activities by counteracting the opposing ubiquitin
            ligase activity of proteins such as HDM2 and TRIM27, involved in
            the respective process. Mutations in this gene have been implicated
            in a neurodevelopmental disorder. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z72499.1, SRR14038192.3340351.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000344836.9/ ENSP00000343535.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-422               AC022167.7         63253-63674
            423-3754            Z72499.1           1-3332
            3755-4140           AY376241.1         3164-3549
            4141-5831           AC022167.7         133372-135062
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.2"
     gene            1..5831
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /note="ubiquitin specific peptidase 7"
                     /db_xref="GeneID:7874"
                     /db_xref="HGNC:HGNC:12630"
                     /db_xref="MIM:602519"
     exon            1..700
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             622..3930
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Herpes virus-associated ubiquitin-specific protease;
                     ubiquitin specific protease 7 (herpes virus-associated);
                     ubiquitin carboxyl-terminal hydrolase 7; ubiquitin
                     thioesterase 7; Chromosome 16p13.2 deletion syndrome;
                     deubiquitinating enzyme 7; ubiquitin-specific-processing
                     protease 7; ubiquitin specific peptidase 7 (herpes
                     virus-associated)"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 7 isoform
                     1"
                     /protein_id="NP_003461.2"
                     /db_xref="CCDS:CCDS32385.1"
                     /db_xref="GeneID:7874"
                     /db_xref="HGNC:HGNC:12630"
                     /db_xref="MIM:602519"
                     /translation="MNHQQQQQQQKAGEQQLSEPEDMEMEAGDTDDPPRITQNPVING
                     NVALSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVLSPPCFVRNLPWKIMVM
                     PRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFH
                     KENDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGYVGLKN
                     QGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGT
                     KKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQC
                     KEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQEAEKG
                     VKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYIL
                     HAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVR
                     HCTNAYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQ
                     VQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWP
                     MQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKD
                     HDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKP
                     NLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDNSELPTAKEYFRDLYHRVDVIF
                     CDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHN
                     YEGTLRDLLQFFKPRQPKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDK
                     HGCVRDLLEECKKAVELGEKASGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRI
                     EEIPLDQVDIDKENEMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSLLDI
                     QEKEFEKFKFAIVMMGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAPKR
                     SRYTYLEKAIKIHN"
     exon            701..805
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            806..1004
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1143
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1232
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1341
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1472
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1527
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1608
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1699
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1782
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1783..1892
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..2049
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2194
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2325
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..2460
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2461..2562
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2563..2668
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2669..2761
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2762..2829
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2830..2930
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2931..3084
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3085..3152
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3153..3261
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3262..3339
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3340..3440
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3441..3540
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3541..3660
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3661..3732
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3733..3823
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3824..5831
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4116..4121
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /note="hexamer: AATGAA"
     polyA_site      4135
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /note="major polyA site"
     regulatory      4414..4419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /note="hexamer: AATAAA"
     polyA_site      4436
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /note="major polyA site"
     regulatory      5131..5136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /note="hexamer: AATAAA"
     polyA_site      5153
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
     regulatory      5810..5815
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /note="hexamer: ATTAAA"
     polyA_site      5831
                     /gene="USP7"
                     /gene_synonym="C16DELp13.2; DEL16P13.2; HAFOUS; HAUSP;
                     TEF1"
                     /note="major polyA site"
ORIGIN      
        1 gacatttcac gccgccgcca ttttgagagc gagccgagcc gagctgccgg gcgccgcgtc
       61 cgctgccgga gccccgacga cgacgccgag gaggcggagg ccgcggctct cggaacgcgg
      121 ccggcccgtc gcccgcccgc tccgccgctc ccgccggccc cagggcccgc aggcccgccg
      181 cggccggcca ggcctcccgt ccgccgcgcc cggcccgagg cggggctgac tccgcggccc
      241 ccgaccggcc gccctccgcc cccggccggc ccgcggcccc gcagccccgg ccccggcccc
      301 ggcggcggga ggcgggcccc gcggcggcgg cggcggcggc cgcagcgagc gacgaggccg
      361 cccggcccgg cccgccggcc gcccgcccgc ctcggcgccg agattgggcg aatcgcgagc
      421 aagtacgtgc gcgtctccct gccgccgccg ccgcccgccg cgggccgccc cggggccgcc
      481 gtcgccgacg acgcgcggga ggaggaggag gaggccgccc cgccgccgcc gccgccgccg
      541 ccgccccggc tcgccgccgc ccgcccgccg ggctcgcagc cccggccccc ggccgcaggc
      601 gaggcccagg ccgcggccga catgaaccac cagcagcagc agcagcagca gaaagcgggc
      661 gagcagcagt tgagcgagcc cgaggacatg gagatggaag cgggagatac agatgaccca
      721 ccaagaatta ctcagaaccc tgtgatcaat gggaatgtgg ccctgagtga tggacacaac
      781 accgcggagg aggacatgga ggatgacacc agttggcgct ccgaggcaac ctttcagttc
      841 actgtggagc gcttcagcag actgagtgag tcggtcctta gccctccgtg ttttgtgcga
      901 aatctgccat ggaagattat ggtgatgcca cgcttttatc cagacagacc acaccaaaaa
      961 agcgtaggat tctttctcca gtgcaatgct gaatctgatt ccacgtcatg gtcttgccat
     1021 gcacaagcag tgctgaagat aataaattac agagatgatg aaaagtcgtt cagtcgtcgt
     1081 attagtcatt tgttcttcca taaagaaaat gattggggat tttccaattt tatggcctgg
     1141 agtgaagtga ccgatcctga gaaaggattt atagatgatg acaaagttac ctttgaagtc
     1201 tttgtacagg cggatgctcc ccatggagtt gcgtgggatt caaagaagca cacaggctac
     1261 gtcggcttaa agaatcaggg agcgacttgt tacatgaaca gcctgctaca gacgttattt
     1321 ttcacgaatc agctacgaaa ggctgtgtac atgatgccaa ccgaggggga tgattcgtct
     1381 aaaagcgtcc ctttagcatt acaaagagtg ttctatgaat tacagcatag tgataaacct
     1441 gtaggaacaa aaaagttaac aaagtcattt gggtgggaaa ctttagatag cttcatgcaa
     1501 catgatgttc aggagctttg tcgagtgttg ctcgataatg tggaaaataa gatgaaaggc
     1561 acctgtgtag agggcaccat acccaaatta ttccgcggca aaatggtgtc ctatatccag
     1621 tgtaaagaag tagactatcg gtctgataga agagaagatt attatgatat ccagctaagt
     1681 atcaaaggaa agaaaaatat atttgaatca tttgtggatt atgtggcagt agaacagctc
     1741 gatggggaca ataaatacga cgctggggaa catggcttac aggaagcaga gaaaggtgtg
     1801 aaattcctaa cattgccacc agtgttacat ctacaactga tgagatttat gtatgaccct
     1861 cagacggacc aaaatatcaa gatcaatgat aggtttgaat tcccagagca gttaccactt
     1921 gatgaatttt tgcaaaaaac agatcctaag gaccctgcaa attatattct tcatgcagtc
     1981 ctggttcata gtggagataa tcatggtgga cattatgtgg tttatctaaa ccccaaaggg
     2041 gatggcaaat ggtgtaaatt tgatgacgac gtggtgtcaa ggtgtactaa agaggaagca
     2101 attgagcaca attatggggg tcacgatgac gacctgtctg ttcgacactg cactaatgct
     2161 tacatgttag tctacatcag ggaatcaaaa ctgagtgaag ttttacaggc ggtcaccgac
     2221 catgatattc ctcagcagtt ggtggagcga ttacaagaag agaaaaggat cgaggctcag
     2281 aagcggaagg agcggcagga agcccatctc tatatgcaag tgcagatagt cgcagaggac
     2341 cagttttgtg gccaccaagg gaatgacatg tacgatgaag aaaaagtgaa atacactgtg
     2401 ttcaaagtat tgaagaactc ctcgcttgct gagtttgttc agagcctctc tcagaccatg
     2461 ggatttccac aagatcaaat tcgattgtgg cccatgcaag caaggagtaa tggaacaaaa
     2521 cgaccagcaa tgttagataa tgaagccgac ggcaataaaa caatgattga gctcagtgat
     2581 aatgaaaacc cttggacaat attcctggaa acagttgatc ccgagctggc tgctagtgga
     2641 gcgaccttac ccaagtttga taaagatcat gatgtaatgt tatttttgaa gatgtatgat
     2701 cccaaaacgc ggagcttgaa ttactgtggg catatctaca caccaatatc ctgtaaaata
     2761 cgtgacttgc tcccagttat gtgtgacaga gcaggattta ttcaagatac tagccttatc
     2821 ctctatgagg aagttaaacc gaatttaaca gagagaattc aggactatga cgtgtctctt
     2881 gataaagccc ttgatgaact aatggatggt gacatcatag tatttcagaa ggatgaccct
     2941 gaaaatgata acagtgaatt acccaccgca aaggagtatt tccgagatct ctaccaccgc
     3001 gttgatgtca ttttctgtga taaaacaatc cctaatgatc ctggatttgt ggttacgtta
     3061 tcaaatagaa tgaattattt tcaggttgca aagacagttg cacagaggct caacacagat
     3121 ccaatgttgc tgcagttttt caagtctcaa ggttataggg atggcccagg taatcctctt
     3181 agacataatt atgaaggtac tttaagagat cttctacagt tcttcaagcc tagacaacct
     3241 aagaaacttt actatcagca gcttaagatg aaaatcacag actttgagaa caggcgaagt
     3301 tttaaatgta tatggttaaa cagccaattt agggaagagg aaataacact atatccagac
     3361 aagcatgggt gtgtccggga cctgttagaa gaatgtaaaa aggccgtgga gcttggggag
     3421 aaagcatcag ggaaacttag gctgctagaa attgtaagct acaaaatcat tggtgttcat
     3481 caagaagatg aactattaga atgtttatct cctgcaacga gccggacgtt tcgaatagag
     3541 gaaatccctt tggaccaggt ggacatagac aaagagaatg agatgcttgt cacagtggcg
     3601 catttccaca aagaggtctt cggaacgttc ggaatcccgt ttttgctgag gatacaccag
     3661 ggcgagcatt ttcgagaagt gatgaagcga atccagagcc tgctggacat ccaggagaag
     3721 gagtttgaga agtttaaatt tgcaattgta atgatgggcc gacaccagta cataaatgaa
     3781 gacgagtatg aagtaaattt gaaagacttt gagccacagc ccggtaatat gtctcatcct
     3841 cggccttggc tagggctcga ccacttcaac aaagccccaa agaggagtcg ctacacttac
     3901 cttgaaaagg ccattaaaat ccataactga tttccaagct ggtgtgttca aggcgaggac
     3961 ggtgtgtggg tggcccctta acagcctaga actttggtgc acgtgccctc tagccgaagt
     4021 cttcagcaag aggattcgct gctggtgtta attttatttt attgaggctg ttcagtttgg
     4081 cttctctgta tctattgact gccctttttg agcaaaatga agatgttttt ataaagcttg
     4141 gatgccaatg agagttattt tatggtaacc acagtgcaag gcaactgtca gcgcaatggg
     4201 ggagaagagg ttagtggatc gggggtccct ggctcaaggt ctctgggctg tccctagtgg
     4261 gcacgagtgg ctcggctgcc ttcctggggt cccgtgcacc agccctgcag ctagcaagtc
     4321 ttgtgtttag gctcgtctga cctatttcct tcagttatac tttcaatgac cttttgtgca
     4381 tctgttaagg caaaacagag aaactcacaa cctaataaat agcgctcttc ccttcattgt
     4441 gtgcattgtc ggcccttcct cgggttctcc tcctccagct gcctgggggc tttttaataa
     4501 acttgtctca cctcgtcagc cactactgtc tgcagcccct ttgcaaagtg gatgcactga
     4561 atacagtccg gacagacatt gtgggggtct ttttattaaa tcaagaacat tgttaaattc
     4621 aattaaggtt tactctgctg ccttggcaga cttacgatct caacagttca tacgagcagg
     4681 tgaaaggatt ataaatagaa tttcgttaaa gtggaacaga cgacaagaaa gccttttagc
     4741 aagagggcat gctcactagt ggttagtaag ctgtcgactt tgtaaaaaag ttaaaaatga
     4801 aaaaaaaagg aaaaatgaat tgtatattta atgaatgaac atgtacaatt tgccactggg
     4861 aggaggttcc tttttgttgg gtgagtctgc aagtgaattt cactgatgtt gatattcatt
     4921 gtgtgtagtt ttatttcggt cccagccccg tttcctttta ttttggagct aatgccagct
     4981 gcgtgtctag ttttgagtgc agtaaaatag aatcagcaaa tcactcttat ttttcatcct
     5041 tttccggtat tttttgggtt gtttctgtgg gagcagtgta caccaactct tcctgtatat
     5101 tgcctttttg ctggaaaatg ttgtatgttg aataaaattt tctataaaaa ttataattca
     5161 gtgagttacg tggaagtgga ggaagatttc tactctccct ggaaacaggc ctgggaaacc
     5221 ttggcatttg taacaaggtt tcactgagat gtacttttcc ttctaattcc gttttgcggg
     5281 ggcagggtct cttgtttctt tttttttttt tttttttttt tagcctctaa ctagtcacat
     5341 ttactcttaa gaaatgaaag gttttccagg agagaactgt gtacaaataa ggtgactgga
     5401 gatgtgacct gatgtgtcac gaggcccttc ggggcggcag gcgctatcgt gggcgtggtc
     5461 cttgcaccgt cccatcggcc ttgccttcca gctccgtggc acggtttcct ggtctttggg
     5521 ccagtgtgta ccttggagtg acttcctttc tcaacttcca ctgcagtgtg tgtgccttct
     5581 gctctgagag ctgccttgtg acccgtgtga tagaaagcag ggagtgaggg tccccgcgga
     5641 cctggccctt ccctccttcc tcccccagaa agaggagtta gagcaggggt gcgagagccg
     5701 ttcgctgtgg gtttgtcttt gaacaaacat taaggtgtct tgtttttgtt ctgggctggg
     5761 ggttggctgt agtcttaggt aactgaaagt tcctactctc ccttaaggta ttaaatgact
     5821 ctttttccaa a
//
