LOCUS       NM_001371398            6381 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript
            variant 5, mRNA.
ACCESSION   NM_001371398 XM_017008318
VERSION     NM_001371398.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Wendrich,K., Gallant,K., Recknagel,S., Petroulia,S., Kazi,N.H.,
            Hane,J.A., Fuhrer,S., Bezstarosti,K., O'Dea,R., Demmers,J. and
            Gersch,M.
  TITLE     Discovery and mechanism of K63-linkage-directed deubiquitinase
            activity in USP53
  JOURNAL   Nat Chem Biol (2024) In press
   PUBMED   39587316
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Cheng,W., Tang,Y., Tong,X., Zhou,Q., Xie,J., Wang,J., Han,Y., Ta,N.
            and Ye,Z.
  TITLE     USP53 activated by H3K27 acetylation regulates cell viability,
            apoptosis and metabolism in esophageal carcinoma via the AMPK
            signaling pathway
  JOURNAL   Carcinogenesis 43 (4), 349-359 (2022)
   PUBMED   34919659
  REMARK    GeneRIF: USP53 activated by H3K27 acetylation regulates cell
            viability, apoptosis and metabolism in esophageal carcinoma via the
            AMPK signaling pathway.
REFERENCE   3  (bases 1 to 6381)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   4  (bases 1 to 6381)
  AUTHORS   Gui,D., Dong,Z., Peng,W., Jiang,W., Huang,G., Liu,G., Ye,Z.,
            Wang,Y., Xu,Z., Fu,J., Luo,S. and Zhao,Y.
  TITLE     Ubiquitin-specific peptidase 53 inhibits the occurrence and
            development of clear cell renal cell carcinoma through NF-kappaB
            pathway inactivation
  JOURNAL   Cancer Med 10 (11), 3674-3688 (2021)
   PUBMED   33973730
  REMARK    GeneRIF: Ubiquitin-specific peptidase 53 inhibits the occurrence
            and development of clear cell renal cell carcinoma through
            NF-kappaB pathway inactivation.
REFERENCE   5  (bases 1 to 6381)
  AUTHORS   Baek,D., Park,K.H., Lee,K.M., Jung,S., Joung,S., Kim,J. and
            Lee,J.W.
  TITLE     Ubiquitin-specific protease 53 promotes osteogenic differentiation
            of human bone marrow-derived mesenchymal stem cells
  JOURNAL   Cell Death Dis 12 (3), 238 (2021)
   PUBMED   33664230
  REMARK    GeneRIF: Ubiquitin-specific protease 53 promotes osteogenic
            differentiation of human bone marrow-derived mesenchymal stem
            cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6381)
  AUTHORS   Lopes,M.C., Hysi,P.G., Verhoeven,V.J., Macgregor,S., Hewitt,A.W.,
            Montgomery,G.W., Cumberland,P., Vingerling,J.R., Young,T.L., van
            Duijn,C.M., Oostra,B., Uitterlinden,A.G., Rahi,J.S., Mackey,D.A.,
            Klaver,C.C., Andrew,T. and Hammond,C.J.
  TITLE     Identification of a candidate gene for astigmatism
  JOURNAL   Invest Ophthalmol Vis Sci 54 (2), 1260-1267 (2013)
   PUBMED   23322567
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6381)
  AUTHORS   Kalsi,G., Kuo,P.H., Aliev,F., Alexander,J., McMichael,O.,
            Patterson,D.G., Walsh,D., Zhao,Z., Schuckit,M., Nurnberger,J. Jr.,
            Edenberg,H., Kramer,J., Hesselbrock,V., Tischfield,J.A.,
            Vladimirov,V., Prescott,C.A., Dick,D.M., Kendler,K.S. and
            Riley,B.P.
  TITLE     A systematic gene-based screen of chr4q22-q32 identifies
            association of a novel susceptibility gene, DKK2, with the
            quantitative trait of alcohol dependence symptom counts
  JOURNAL   Hum Mol Genet 19 (12), 2497-2506 (2010)
   PUBMED   20332099
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Hum Mol Genet. 2010 Oct 15;19(20):4121. Hesselbrock,
            Victor [added]; Tischfield, Jay A [added]]
REFERENCE   8  (bases 1 to 6381)
  AUTHORS   Ozyildirim,A.M., Wistow,G.J., Gao,J., Wang,J., Dickinson,D.P.,
            Frierson,H.F. Jr. and Laurie,G.W.
  TITLE     The lacrimal gland transcriptome is an unusually rich source of
            rare and poorly characterized gene transcripts
  JOURNAL   Invest Ophthalmol Vis Sci 46 (5), 1572-1580 (2005)
   PUBMED   15851553
REFERENCE   9  (bases 1 to 6381)
  AUTHORS   Quesada,V., Diaz-Perales,A., Gutierrez-Fernandez,A., Garabaya,C.,
            Cal,S. and Lopez-Otin,C.
  TITLE     Cloning and enzymatic analysis of 22 novel human ubiquitin-specific
            proteases
  JOURNAL   Biochem Biophys Res Commun 314 (1), 54-62 (2004)
   PUBMED   14715245
REFERENCE   10 (bases 1 to 6381)
  AUTHORS   Amendola,M. and Squires,J.E.
  TITLE     Pediatric Genetic Cholestatic Liver Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   36108118
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092656.1.
            
            On Jul 11, 2019 this sequence version replaced XM_017008318.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14372079.502892.1,
                                           SRR14372080.2282772.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-161               AC092656.1         146709-146869       c
            162-285             AC092656.1         141909-142032       c
            286-407             AC092656.1         124171-124292       c
            408-1093            AC092656.1         119679-120364       c
            1094-1186           AC092656.1         114153-114245       c
            1187-1321           AC092656.1         110700-110834       c
            1322-1435           AC092656.1         103223-103336       c
            1436-1518           AC092656.1         103059-103141       c
            1519-1624           AC092656.1         99657-99762         c
            1625-1771           AC092656.1         98929-99075         c
            1772-1921           AC092656.1         97718-97867         c
            1922-2084           AC092656.1         92100-92262         c
            2085-2237           AC092656.1         91162-91314         c
            2238-2384           AC092656.1         89745-89891         c
            2385-3123           AC092656.1         87548-88286         c
            3124-3200           AC092656.1         85874-85950         c
            3201-6381           AC092656.1         64064-67244         c
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q26"
     gene            1..6381
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="ubiquitin specific peptidase 53"
                     /db_xref="GeneID:54532"
                     /db_xref="HGNC:HGNC:29255"
                     /db_xref="MIM:617431"
     exon            1..161
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            162..285
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            286..407
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            408..1093
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     CDS             950..3352
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="isoform 3 is encoded by transcript variant 5;
                     ubiquitin specific protease 53; ubiquitin specific
                     proteinase 53; inactive ubiquitin carboxyl-terminal
                     hydrolase 53; inactive ubiquitin-specific peptidase 53"
                     /codon_start=1
                     /product="inactive ubiquitin carboxyl-terminal hydrolase
                     53 isoform 3"
                     /protein_id="NP_001358327.1"
                     /db_xref="CCDS:CCDS93614.1"
                     /db_xref="GeneID:54532"
                     /db_xref="HGNC:HGNC:29255"
                     /db_xref="MIM:617431"
                     /translation="MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLN
                     SAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHAL
                     AESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLY
                     EQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTT
                     DDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLF
                     YRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVS
                     KCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLK
                     ENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECAL
                     KAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLY
                     HSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDS
                     RDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPS
                     FSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQ
                     RTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDC
                     TSLQSQHHLEDHMYMKTMESYFLHPVYKYPRTIMQENISTSQMNRNLKNRMNANFLSG
                     LI"
     exon            1094..1186
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1321
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1435
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1518
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1624
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1771
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1921
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1922..2084
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2237
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            2238..2384
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            2385..3123
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            3124..3200
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            3201..6381
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5475..5480
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="hexamer: AATAAA"
     polyA_site      5492
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="major polyA site"
     regulatory      5584..5589
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="hexamer: TATAAA"
     polyA_site      5605
                     /gene="USP53"
                     /gene_synonym="PFIC7"
     regulatory      6360..6365
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="hexamer: GATAAA"
     polyA_site      6381
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="major polyA site"
ORIGIN      
        1 agttcccggt gagcctcggt actgtggcag cagtcagtgt gtctggcggg tgtcggcgtg
       61 aagcggggct gggccagcgg gaggtagctc tgtgggagtg gaaggcctgt atttctctac
      121 ctggaccgca cgctcctcgc gcaaggaggg tagagctcaa gatgcttggg atttgcttta
      181 cattctaagt ggatttggag aagagacaat aaagaataag cagtcaattc tataatatat
      241 ttagaaggag aaaagtgcca aggaggaatg aaaaagtaga acaagagaca gcatcttgct
      301 gccacccagg ctggagtgca gtggtgcagt cttagcttac tgcagcctca aacttctggg
      361 ctcaagtaat cctcccgcct cagcttcctg agtagctgag attacagatt tgctttgagg
      421 tcacattgat atgaaatatg gagtcacctt ttataacttc acctgggaaa gagagagaaa
      481 actttcttgt taaaaaaaca taaggaaaac aaacaaccaa aggaatcatg ccaaatgcca
      541 actctacatc tttttgtatt attaacatat tttatgcatc aagacaaatg gcttatgcat
      601 taaaggggat gattcgttca tcagtatttt tagccattaa aaaaaatcgt tttcatatta
      661 accttataca gctcccataa aatttaacac atataaacat ctttaacgcc ttgtttaaaa
      721 tagctttctt ttttagtgct tagaacttca atgtttatgc accctattgt tacttgtcag
      781 agtctttaag agtttataac atcaggaaag atatggactt ggaaacttac attattaaaa
      841 tagactgcag tggatttaat tggacaattc aagacatcca ttttattgtc caaaatatta
      901 cataaaagtg tacagttttt agcctaaatg caaacaaagt tgcttgaaaa tggcatgggt
      961 aaaattctta cggaaacctg gtggcaatct tggaaaagtt tatcagcctg gaagtatgct
     1021 atcactagcc cctaccaaag gcttgttaaa tgaaccagga caaaacagct gctttcttaa
     1081 tagcgctgta caggttttat ggcaattgga tatattccga cgaagcttgc gggttttgac
     1141 tggacatgtt tgtcagggag atgcctgtat attttgtgca ttgaagacga tatttgcaca
     1201 gttccaacac agtcgagaaa aagcacttcc ctcagataac ataaggcatg ctcttgcaga
     1261 aagtttcaaa gatgagcagc gatttcaact tggccttatg gatgatgctg cggagtgctt
     1321 tgaaaatatg ttggagagga ttcattttca catagtgcca agcagagatg cagacatgtg
     1381 tacctctaaa tcttgtatca ctcaccagaa gtttgctatg actctgtatg aacagtgtgt
     1441 gtgtcgtagc tgtggagcat cgtcagatcc tctacctttt acagaatttg tgcggtacat
     1501 ttctacaaca gccttatgca atgaggttga aagaatgttg gaaaggcatg aacgctttaa
     1561 acctgaaatg tttgcagaat tgctacaagc agcaaataca acagatgact ataggaaatg
     1621 tcctagtaac tgtggccaaa aaataaaaat tcgccgtgtt ttaatgaatt gcccagagat
     1681 tgttacaatt ggtttagtct gggactccga gcattctgac ttgaccgaag ctgttgttcg
     1741 gaatctagca acacatcttt atcttcctgg gcttttttat agagttactg atgaaaatgc
     1801 caaaaatagt gaacttaacc ttgttggtat gatctgctac accagccaac attattgtgc
     1861 ctttgcattt cacaccaaaa gttccaaatg ggtatttttt gatgatgcaa atgtgaaaga
     1921 gattggaact agatggaaag atgttgtctc caaatgcatt cgatgccact ttcagccact
     1981 acttttgttt tatgcaaacc cagatggcac agcagtttct actgaggatg cactcaggca
     2041 ggtcatcagc tggtcacatt acaaatctgt tgcagaaaat atgggatgtg aaaagcctgt
     2101 aattcataag tcagataatt taaaagaaaa tggatttggt gatcaggcaa agcagagaga
     2161 aaatcagaaa tttccaactg ataatatttc atcatctaat cggagccaca gtcacacagg
     2221 tgtagggaaa ggaccagcta agttaagtca cattgatcaa agggaaaaga taaaagacat
     2281 ttccagagaa tgtgctctga aagctattga acagaaaaac ttactttctt cacaaaggaa
     2341 agatttagag aagggacaaa gaaaagattt aggacgacat agagatttgg ttgatgaaga
     2401 cctttcacat ttccaatctg gatcacctcc tgccccaaat ggttttaaac aacatgggaa
     2461 tccacatcta tatcatagtc aaggaaaagg atcatataaa catgaccgag ttgtacctca
     2521 gagtcgagct tctgcacaaa taataagttc aagtaaatcc cagattcttg ctccaggaga
     2581 gaaaataact ggcaaagtta agagtgacaa tggcactgga tatgacacag acagcagcca
     2641 agattctagg gatagaggaa acagctgtga tagcagcagt aaaagccgga accgaggttg
     2701 gaaacctatg agagaaacat taaatgttga tagtattttt agtgaaagtg aaaaaagaca
     2761 gcatagtcca agacataaac caaatatcag taataagcct aaatctagca aggatccgag
     2821 ttttagtaat tggccaaaag agaatccaaa gcaaaaaggt ttaatgacca tatatgaaga
     2881 tgaaatgaag caggaaatag gaagcagaag ttcccttgaa tctaatggaa aaggagcaga
     2941 gaaaaataaa ggccttgtag agggtaaagt gcatggtgat aattggcaga tgcaaaggac
     3001 tgagtctgga tatgaaagca gtgatcacat cagtaatggt tctactaatt tggactcacc
     3061 tgttatcgat ggaaatggta cagtaatgga tatcagtggt gttaaagaaa cagtatgctt
     3121 cagtgaccag attacgacaa gcaacctaaa taaagaacgt ggggactgta cctcccttca
     3181 gagccaacat cacttagaag atcacatgta catgaagaca atggaaagtt atttccttca
     3241 tccagtctac aaatacccaa ggaccataat gcaagagaac atatccacca gtcagatgaa
     3301 cagaaacttg aaaaaccgaa tgaatgcaaa ttttctgagt ggcttaatat agaaaattct
     3361 gagagaacag gtttgccttt tcacgttgat aactctgctt ctgggaagag agtgaacagt
     3421 aatgaaccat cttcattatg gtcttcacac ctaagaactg ttgggttaaa gccagaaact
     3481 gctcctctca tccagcaaca aaatatcatg gatcaatgtt actttgagaa ctctctatcc
     3541 acagaatgta taattcggtc agccagcaga tctgatgggt gtcagatgcc aaaacttttt
     3601 tgccagaatc taccaccccc tttgccacca aagaaatatg ctataaccag tgtgccacag
     3661 tcagagaaaa gcgaatctac acctgatgtc aaacttacag aggtgtttaa agctacctct
     3721 catcttccga agcacagttt aagtacagct tcagaaccaa gtttagaagt gagtacacat
     3781 atgaatgatg aaagacataa agaaacattt caagtgagag aatgttttgg caacacacca
     3841 aactgtccat ccagctcctc aactaacgat tttcaggcaa actcaggtgc cattgatgca
     3901 ttttgccaac cagaactaga ctctatttct acctgtccaa atgagacagt ttcattaact
     3961 acctattttt cagttgatag ctgcatgacg gatacatata gattgaaata ccatcagagg
     4021 cccaagctct cttttccaga gagcagtggc ttttgtaata attcactatc ttagagtgaa
     4081 aaaggactag acctgtgtta cataataatc ttggttcaag ctgcccttct gaacaaagat
     4141 ataaacctag catacattgt aatagataac tggtaaaact gaccaacttt tacttctcag
     4201 aggccattta aatataatag gaacctactg accaaaccta gtgatacata aaattaaagc
     4261 ctgtggcatt tttaaagttg ttaatcacta tacatatgta tgtgtatatg tgtatacaca
     4321 tatataattt tatgatcaat atcttagata ttttagaaat tccctttgaa tagtcttggc
     4381 gtgccgtgaa aaatagaaaa tcagggagat ataataattc atttgtcata tgctacagtt
     4441 gaataaaaat taaatttgtc cccctatttt gtggccagta gactgggaag tattaaacta
     4501 accagtactc atgtatggag attataaact atgctaattg aaaagtacca ggtgatacat
     4561 agcctggtac ttttttgtca aagaattgct ttatgaagaa gcactttcta atgggtaacc
     4621 agaaaagggc ttcacccatt tgtttttgac ttctatgata gtcactgcat atgatccctt
     4681 taagtgtctt taaaaaaaat gacttatgaa tttgatagca tttggggaaa aaaagcctta
     4741 tatgattaac aatttgtaag ttttcaagat cggatggaat ttaattttgc tcctagaatt
     4801 tttgtcttca gaatatctag ttaagataaa ttaggccttt gactattata ggtattcata
     4861 aatgctactt tagccatgta agttaaaaag ttaaaatact taaactttag tgtatttatt
     4921 tatctcgtta ctttttttct aattttatat gaaatgtgaa aggtctttat tttggtttgg
     4981 tttactttgg gctgctaacc accagtgtta ggaattaatg tagggcaccc tcttattggg
     5041 aatgtcactg taattgccta cattttcttt gctgtaatat tacctgctta taggttgtca
     5101 acccatttta tctgaatttg aaaatctatc taggtttcca gattaaattt ctacctttca
     5161 tatagagagt ttaattttaa taaaatgttt aattagagca tccttccctt ttattctact
     5221 ttgaacaaat agtaatttta ccccaactac ttttcatgaa gagtgctttg aaaattaaag
     5281 gaatttttat tattatggta accttatgag taaaaataag ttactttaat tgctttattt
     5341 cgttagttgt cattttattt tgtactatgc tggtaaacta attgtggttt agaatagttt
     5401 tatgttcact cctgataact gagaaacaga actaaagttt tccttacatt gctaaatgga
     5461 taaacctcat attcaataaa ctagtaaatg tagccacttg actgattgcg taaatcgtgt
     5521 taaaacattc taaaatgttg taactaattt aatgtgaata ctgttaaaca gaaaataatt
     5581 gcttataaaa gtctaacttc acccagttac caagtcttgg ccaaatttta tcttctgcct
     5641 atagttttta ctagcctttt taaactataa tagtgagtga tcattacttt atattctcgt
     5701 aagttagaaa tacttgaatg ccaaattaat tttgttttaa gaaagcttat ttttaacata
     5761 attttttagt gtggagggtt tgtaaataat gtaaagtttg taaacctgta tttaaaatac
     5821 caaagttttt cttcttttat aacattgtaa gatactagaa aaatgggctt caagtttttt
     5881 aatagctttc acagtttgtg tagttttttt tttctatttg gttaagatgt gagttggaaa
     5941 tgtattattt gaaacattta aactaatttt tgtatttaac agcagtctca gacatttgtc
     6001 ttttcaactc agttgagttg tagatatcaa atagaaatgt ccagttttgc ctcttttaag
     6061 tactgtccag ttgaaacctc ctgactcatg tactactaca caaactagaa aatgcccatt
     6121 tatttttgtt gattcaacac tccagataca gattgataca tatatactaa tcattttatt
     6181 tcatgtaaaa gttttacatc tagggtgcct atttattttt atagatgctt tcatccagtt
     6241 ataagcttat tataactttg taaatatttc agccaacagt tgtaatgttt aaaatactta
     6301 ccttcagaat gttatatttt catgtgaaat caatttcttc taattcaaaa aaattttaag
     6361 ataaactgat ttttaagttt a
//
