LOCUS       NM_001371395            6631 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens ubiquitin specific peptidase 53 (USP53), transcript
            variant 1, mRNA.
ACCESSION   NM_001371395 XM_017008312
VERSION     NM_001371395.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Wendrich,K., Gallant,K., Recknagel,S., Petroulia,S., Kazi,N.H.,
            Hane,J.A., Fuhrer,S., Bezstarosti,K., O'Dea,R., Demmers,J. and
            Gersch,M.
  TITLE     Discovery and mechanism of K63-linkage-directed deubiquitinase
            activity in USP53
  JOURNAL   Nat Chem Biol (2024) In press
   PUBMED   39587316
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Cheng,W., Tang,Y., Tong,X., Zhou,Q., Xie,J., Wang,J., Han,Y., Ta,N.
            and Ye,Z.
  TITLE     USP53 activated by H3K27 acetylation regulates cell viability,
            apoptosis and metabolism in esophageal carcinoma via the AMPK
            signaling pathway
  JOURNAL   Carcinogenesis 43 (4), 349-359 (2022)
   PUBMED   34919659
  REMARK    GeneRIF: USP53 activated by H3K27 acetylation regulates cell
            viability, apoptosis and metabolism in esophageal carcinoma via the
            AMPK signaling pathway.
REFERENCE   3  (bases 1 to 6631)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   4  (bases 1 to 6631)
  AUTHORS   Gui,D., Dong,Z., Peng,W., Jiang,W., Huang,G., Liu,G., Ye,Z.,
            Wang,Y., Xu,Z., Fu,J., Luo,S. and Zhao,Y.
  TITLE     Ubiquitin-specific peptidase 53 inhibits the occurrence and
            development of clear cell renal cell carcinoma through NF-kappaB
            pathway inactivation
  JOURNAL   Cancer Med 10 (11), 3674-3688 (2021)
   PUBMED   33973730
  REMARK    GeneRIF: Ubiquitin-specific peptidase 53 inhibits the occurrence
            and development of clear cell renal cell carcinoma through
            NF-kappaB pathway inactivation.
REFERENCE   5  (bases 1 to 6631)
  AUTHORS   Baek,D., Park,K.H., Lee,K.M., Jung,S., Joung,S., Kim,J. and
            Lee,J.W.
  TITLE     Ubiquitin-specific protease 53 promotes osteogenic differentiation
            of human bone marrow-derived mesenchymal stem cells
  JOURNAL   Cell Death Dis 12 (3), 238 (2021)
   PUBMED   33664230
  REMARK    GeneRIF: Ubiquitin-specific protease 53 promotes osteogenic
            differentiation of human bone marrow-derived mesenchymal stem
            cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6631)
  AUTHORS   Lopes,M.C., Hysi,P.G., Verhoeven,V.J., Macgregor,S., Hewitt,A.W.,
            Montgomery,G.W., Cumberland,P., Vingerling,J.R., Young,T.L., van
            Duijn,C.M., Oostra,B., Uitterlinden,A.G., Rahi,J.S., Mackey,D.A.,
            Klaver,C.C., Andrew,T. and Hammond,C.J.
  TITLE     Identification of a candidate gene for astigmatism
  JOURNAL   Invest Ophthalmol Vis Sci 54 (2), 1260-1267 (2013)
   PUBMED   23322567
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6631)
  AUTHORS   Kalsi,G., Kuo,P.H., Aliev,F., Alexander,J., McMichael,O.,
            Patterson,D.G., Walsh,D., Zhao,Z., Schuckit,M., Nurnberger,J. Jr.,
            Edenberg,H., Kramer,J., Hesselbrock,V., Tischfield,J.A.,
            Vladimirov,V., Prescott,C.A., Dick,D.M., Kendler,K.S. and
            Riley,B.P.
  TITLE     A systematic gene-based screen of chr4q22-q32 identifies
            association of a novel susceptibility gene, DKK2, with the
            quantitative trait of alcohol dependence symptom counts
  JOURNAL   Hum Mol Genet 19 (12), 2497-2506 (2010)
   PUBMED   20332099
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Erratum:[Hum Mol Genet. 2010 Oct 15;19(20):4121. Hesselbrock,
            Victor [added]; Tischfield, Jay A [added]]
REFERENCE   8  (bases 1 to 6631)
  AUTHORS   Ozyildirim,A.M., Wistow,G.J., Gao,J., Wang,J., Dickinson,D.P.,
            Frierson,H.F. Jr. and Laurie,G.W.
  TITLE     The lacrimal gland transcriptome is an unusually rich source of
            rare and poorly characterized gene transcripts
  JOURNAL   Invest Ophthalmol Vis Sci 46 (5), 1572-1580 (2005)
   PUBMED   15851553
REFERENCE   9  (bases 1 to 6631)
  AUTHORS   Quesada,V., Diaz-Perales,A., Gutierrez-Fernandez,A., Garabaya,C.,
            Cal,S. and Lopez-Otin,C.
  TITLE     Cloning and enzymatic analysis of 22 novel human ubiquitin-specific
            proteases
  JOURNAL   Biochem Biophys Res Commun 314 (1), 54-62 (2004)
   PUBMED   14715245
REFERENCE   10 (bases 1 to 6631)
  AUTHORS   Amendola,M. and Squires,J.E.
  TITLE     Pediatric Genetic Cholestatic Liver Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   36108118
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC092656.1.
            
            On Jul 11, 2019 this sequence version replaced XM_017008312.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CR749490.1, SRR14038197.283008.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000692078.1/ ENSP00000509606.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-161               AC092656.1         146709-146869       c
            162-314             AC092656.1         145360-145512       c
            315-438             AC092656.1         141909-142032       c
            439-560             AC092656.1         124171-124292       c
            561-1246            AC092656.1         119679-120364       c
            1247-1339           AC092656.1         114153-114245       c
            1340-1474           AC092656.1         110700-110834       c
            1475-1588           AC092656.1         103223-103336       c
            1589-1671           AC092656.1         103059-103141       c
            1672-1777           AC092656.1         99657-99762         c
            1778-1924           AC092656.1         98929-99075         c
            1925-2074           AC092656.1         97718-97867         c
            2075-2237           AC092656.1         92100-92262         c
            2238-2390           AC092656.1         91162-91314         c
            2391-2537           AC092656.1         89745-89891         c
            2538-3276           AC092656.1         87548-88286         c
            3277-3353           AC092656.1         85874-85950         c
            3354-3450           AC092656.1         68321-68417         c
            3451-6631           AC092656.1         64064-67244         c
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q26"
     gene            1..6631
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="ubiquitin specific peptidase 53"
                     /db_xref="GeneID:54532"
                     /db_xref="HGNC:HGNC:29255"
                     /db_xref="MIM:617431"
     exon            1..161
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            162..314
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            315..438
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            439..560
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            561..1246
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     CDS             1103..4324
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin specific protease 53; ubiquitin specific
                     proteinase 53; inactive ubiquitin carboxyl-terminal
                     hydrolase 53; inactive ubiquitin-specific peptidase 53"
                     /codon_start=1
                     /product="inactive ubiquitin carboxyl-terminal hydrolase
                     53 isoform 1"
                     /protein_id="NP_001358324.1"
                     /db_xref="CCDS:CCDS43265.1"
                     /db_xref="GeneID:54532"
                     /db_xref="HGNC:HGNC:29255"
                     /db_xref="MIM:617431"
                     /translation="MAWVKFLRKPGGNLGKVYQPGSMLSLAPTKGLLNEPGQNSCFLN
                     SAVQVLWQLDIFRRSLRVLTGHVCQGDACIFCALKTIFAQFQHSREKALPSDNIRHAL
                     AESFKDEQRFQLGLMDDAAECFENMLERIHFHIVPSRDADMCTSKSCITHQKFAMTLY
                     EQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMLERHERFKPEMFAELLQAANTT
                     DDYRKCPSNCGQKIKIRRVLMNCPEIVTIGLVWDSEHSDLTEAVVRNLATHLYLPGLF
                     YRVTDENAKNSELNLVGMICYTSQHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVS
                     KCIRCHFQPLLLFYANPDGTAVSTEDALRQVISWSHYKSVAENMGCEKPVIHKSDNLK
                     ENGFGDQAKQRENQKFPTDNISSSNRSHSHTGVGKGPAKLSHIDQREKIKDISRECAL
                     KAIEQKNLLSSQRKDLEKGQRKDLGRHRDLVDEDLSHFQSGSPPAPNGFKQHGNPHLY
                     HSQGKGSYKHDRVVPQSRASAQIISSSKSQILAPGEKITGKVKSDNGTGYDTDSSQDS
                     RDRGNSCDSSSKSRNRGWKPMRETLNVDSIFSESEKRQHSPRHKPNISNKPKSSKDPS
                     FSNWPKENPKQKGLMTIYEDEMKQEIGSRSSLESNGKGAEKNKGLVEGKVHGDNWQMQ
                     RTESGYESSDHISNGSTNLDSPVIDGNGTVMDISGVKETVCFSDQITTSNLNKERGDC
                     TSLQSQHHLEGFRKELRNLEAGYKSHEFHPESHLQIKNHLIKRSHVHEDNGKLFPSSS
                     LQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIENSERTGLPFHVDNSASGKRVNS
                     NEPSSLWSSHLRTVGLKPETAPLIQQQNIMDQCYFENSLSTECIIRSASRSDGCQMPK
                     LFCQNLPPPLPPKKYAITSVPQSEKSESTPDVKLTEVFKATSHLPKHSLSTASEPSLE
                     VSTHMNDERHKETFQVRECFGNTPNCPSSSSTNDFQANSGAIDAFCQPELDSISTCPN
                     ETVSLTTYFSVDSCMTDTYRLKYHQRPKLSFPESSGFCNNSLS"
     exon            1247..1339
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1474
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1588
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1671
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1777
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1924
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            1925..2074
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            2075..2237
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            2238..2390
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            2391..2537
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            2538..3276
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            3277..3353
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            3354..3450
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     exon            3451..6631
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5725..5730
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="hexamer: AATAAA"
     polyA_site      5742
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="major polyA site"
     regulatory      5834..5839
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="hexamer: TATAAA"
     polyA_site      5855
                     /gene="USP53"
                     /gene_synonym="PFIC7"
     regulatory      6610..6615
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="hexamer: GATAAA"
     polyA_site      6631
                     /gene="USP53"
                     /gene_synonym="PFIC7"
                     /note="major polyA site"
ORIGIN      
        1 agttcccggt gagcctcggt actgtggcag cagtcagtgt gtctggcggg tgtcggcgtg
       61 aagcggggct gggccagcgg gaggtagctc tgtgggagtg gaaggcctgt atttctctac
      121 ctggaccgca cgctcctcgc gcaaggaggg tagagctcaa gttccgtgat gatggtctaa
      181 gcaaagaccc ttcgtcctgt taaagataaa ttaaaaaaaa aaaaaaaaaa ccaaagggaa
      241 caaaacttac tctgcaaaag taatatatga tttacctgct gttgtcataa gaattccaaa
      301 tagacaaact cggtatgctt gggatttgct ttacattcta agtggatttg gagaagagac
      361 aataaagaat aagcagtcaa ttctataata tatttagaag gagaaaagtg ccaaggagga
      421 atgaaaaagt agaacaagag acagcatctt gctgccaccc aggctggagt gcagtggtgc
      481 agtcttagct tactgcagcc tcaaacttct gggctcaagt aatcctcccg cctcagcttc
      541 ctgagtagct gagattacag atttgctttg aggtcacatt gatatgaaat atggagtcac
      601 cttttataac ttcacctggg aaagagagag aaaactttct tgttaaaaaa acataaggaa
      661 aacaaacaac caaaggaatc atgccaaatg ccaactctac atctttttgt attattaaca
      721 tattttatgc atcaagacaa atggcttatg cattaaaggg gatgattcgt tcatcagtat
      781 ttttagccat taaaaaaaat cgttttcata ttaaccttat acagctccca taaaatttaa
      841 cacatataaa catctttaac gccttgttta aaatagcttt cttttttagt gcttagaact
      901 tcaatgttta tgcaccctat tgttacttgt cagagtcttt aagagtttat aacatcagga
      961 aagatatgga cttggaaact tacattatta aaatagactg cagtggattt aattggacaa
     1021 ttcaagacat ccattttatt gtccaaaata ttacataaaa gtgtacagtt tttagcctaa
     1081 atgcaaacaa agttgcttga aaatggcatg ggtaaaattc ttacggaaac ctggtggcaa
     1141 tcttggaaaa gtttatcagc ctggaagtat gctatcacta gcccctacca aaggcttgtt
     1201 aaatgaacca ggacaaaaca gctgctttct taatagcgct gtacaggttt tatggcaatt
     1261 ggatatattc cgacgaagct tgcgggtttt gactggacat gtttgtcagg gagatgcctg
     1321 tatattttgt gcattgaaga cgatatttgc acagttccaa cacagtcgag aaaaagcact
     1381 tccctcagat aacataaggc atgctcttgc agaaagtttc aaagatgagc agcgatttca
     1441 acttggcctt atggatgatg ctgcggagtg ctttgaaaat atgttggaga ggattcattt
     1501 tcacatagtg ccaagcagag atgcagacat gtgtacctct aaatcttgta tcactcacca
     1561 gaagtttgct atgactctgt atgaacagtg tgtgtgtcgt agctgtggag catcgtcaga
     1621 tcctctacct tttacagaat ttgtgcggta catttctaca acagccttat gcaatgaggt
     1681 tgaaagaatg ttggaaaggc atgaacgctt taaacctgaa atgtttgcag aattgctaca
     1741 agcagcaaat acaacagatg actataggaa atgtcctagt aactgtggcc aaaaaataaa
     1801 aattcgccgt gttttaatga attgcccaga gattgttaca attggtttag tctgggactc
     1861 cgagcattct gacttgaccg aagctgttgt tcggaatcta gcaacacatc tttatcttcc
     1921 tgggcttttt tatagagtta ctgatgaaaa tgccaaaaat agtgaactta accttgttgg
     1981 tatgatctgc tacaccagcc aacattattg tgcctttgca tttcacacca aaagttccaa
     2041 atgggtattt tttgatgatg caaatgtgaa agagattgga actagatgga aagatgttgt
     2101 ctccaaatgc attcgatgcc actttcagcc actacttttg ttttatgcaa acccagatgg
     2161 cacagcagtt tctactgagg atgcactcag gcaggtcatc agctggtcac attacaaatc
     2221 tgttgcagaa aatatgggat gtgaaaagcc tgtaattcat aagtcagata atttaaaaga
     2281 aaatggattt ggtgatcagg caaagcagag agaaaatcag aaatttccaa ctgataatat
     2341 ttcatcatct aatcggagcc acagtcacac aggtgtaggg aaaggaccag ctaagttaag
     2401 tcacattgat caaagggaaa agataaaaga catttccaga gaatgtgctc tgaaagctat
     2461 tgaacagaaa aacttacttt cttcacaaag gaaagattta gagaagggac aaagaaaaga
     2521 tttaggacga catagagatt tggttgatga agacctttca catttccaat ctggatcacc
     2581 tcctgcccca aatggtttta aacaacatgg gaatccacat ctatatcata gtcaaggaaa
     2641 aggatcatat aaacatgacc gagttgtacc tcagagtcga gcttctgcac aaataataag
     2701 ttcaagtaaa tcccagattc ttgctccagg agagaaaata actggcaaag ttaagagtga
     2761 caatggcact ggatatgaca cagacagcag ccaagattct agggatagag gaaacagctg
     2821 tgatagcagc agtaaaagcc ggaaccgagg ttggaaacct atgagagaaa cattaaatgt
     2881 tgatagtatt tttagtgaaa gtgaaaaaag acagcatagt ccaagacata aaccaaatat
     2941 cagtaataag cctaaatcta gcaaggatcc gagttttagt aattggccaa aagagaatcc
     3001 aaagcaaaaa ggtttaatga ccatatatga agatgaaatg aagcaggaaa taggaagcag
     3061 aagttccctt gaatctaatg gaaaaggagc agagaaaaat aaaggccttg tagagggtaa
     3121 agtgcatggt gataattggc agatgcaaag gactgagtct ggatatgaaa gcagtgatca
     3181 catcagtaat ggttctacta atttggactc acctgttatc gatggaaatg gtacagtaat
     3241 ggatatcagt ggtgttaaag aaacagtatg cttcagtgac cagattacga caagcaacct
     3301 aaataaagaa cgtggggact gtacctccct tcagagccaa catcacttag aaggctttag
     3361 aaaagaactc aggaatttgg aagcaggcta taaatctcat gaattccacc cagaatcaca
     3421 tttacaaata aaaaatcatt tgataaaaag atcacatgta catgaagaca atggaaagtt
     3481 atttccttca tccagtctac aaatacccaa ggaccataat gcaagagaac atatccacca
     3541 gtcagatgaa cagaaacttg aaaaaccgaa tgaatgcaaa ttttctgagt ggcttaatat
     3601 agaaaattct gagagaacag gtttgccttt tcacgttgat aactctgctt ctgggaagag
     3661 agtgaacagt aatgaaccat cttcattatg gtcttcacac ctaagaactg ttgggttaaa
     3721 gccagaaact gctcctctca tccagcaaca aaatatcatg gatcaatgtt actttgagaa
     3781 ctctctatcc acagaatgta taattcggtc agccagcaga tctgatgggt gtcagatgcc
     3841 aaaacttttt tgccagaatc taccaccccc tttgccacca aagaaatatg ctataaccag
     3901 tgtgccacag tcagagaaaa gcgaatctac acctgatgtc aaacttacag aggtgtttaa
     3961 agctacctct catcttccga agcacagttt aagtacagct tcagaaccaa gtttagaagt
     4021 gagtacacat atgaatgatg aaagacataa agaaacattt caagtgagag aatgttttgg
     4081 caacacacca aactgtccat ccagctcctc aactaacgat tttcaggcaa actcaggtgc
     4141 cattgatgca ttttgccaac cagaactaga ctctatttct acctgtccaa atgagacagt
     4201 ttcattaact acctattttt cagttgatag ctgcatgacg gatacatata gattgaaata
     4261 ccatcagagg cccaagctct cttttccaga gagcagtggc ttttgtaata attcactatc
     4321 ttagagtgaa aaaggactag acctgtgtta cataataatc ttggttcaag ctgcccttct
     4381 gaacaaagat ataaacctag catacattgt aatagataac tggtaaaact gaccaacttt
     4441 tacttctcag aggccattta aatataatag gaacctactg accaaaccta gtgatacata
     4501 aaattaaagc ctgtggcatt tttaaagttg ttaatcacta tacatatgta tgtgtatatg
     4561 tgtatacaca tatataattt tatgatcaat atcttagata ttttagaaat tccctttgaa
     4621 tagtcttggc gtgccgtgaa aaatagaaaa tcagggagat ataataattc atttgtcata
     4681 tgctacagtt gaataaaaat taaatttgtc cccctatttt gtggccagta gactgggaag
     4741 tattaaacta accagtactc atgtatggag attataaact atgctaattg aaaagtacca
     4801 ggtgatacat agcctggtac ttttttgtca aagaattgct ttatgaagaa gcactttcta
     4861 atgggtaacc agaaaagggc ttcacccatt tgtttttgac ttctatgata gtcactgcat
     4921 atgatccctt taagtgtctt taaaaaaaat gacttatgaa tttgatagca tttggggaaa
     4981 aaaagcctta tatgattaac aatttgtaag ttttcaagat cggatggaat ttaattttgc
     5041 tcctagaatt tttgtcttca gaatatctag ttaagataaa ttaggccttt gactattata
     5101 ggtattcata aatgctactt tagccatgta agttaaaaag ttaaaatact taaactttag
     5161 tgtatttatt tatctcgtta ctttttttct aattttatat gaaatgtgaa aggtctttat
     5221 tttggtttgg tttactttgg gctgctaacc accagtgtta ggaattaatg tagggcaccc
     5281 tcttattggg aatgtcactg taattgccta cattttcttt gctgtaatat tacctgctta
     5341 taggttgtca acccatttta tctgaatttg aaaatctatc taggtttcca gattaaattt
     5401 ctacctttca tatagagagt ttaattttaa taaaatgttt aattagagca tccttccctt
     5461 ttattctact ttgaacaaat agtaatttta ccccaactac ttttcatgaa gagtgctttg
     5521 aaaattaaag gaatttttat tattatggta accttatgag taaaaataag ttactttaat
     5581 tgctttattt cgttagttgt cattttattt tgtactatgc tggtaaacta attgtggttt
     5641 agaatagttt tatgttcact cctgataact gagaaacaga actaaagttt tccttacatt
     5701 gctaaatgga taaacctcat attcaataaa ctagtaaatg tagccacttg actgattgcg
     5761 taaatcgtgt taaaacattc taaaatgttg taactaattt aatgtgaata ctgttaaaca
     5821 gaaaataatt gcttataaaa gtctaacttc acccagttac caagtcttgg ccaaatttta
     5881 tcttctgcct atagttttta ctagcctttt taaactataa tagtgagtga tcattacttt
     5941 atattctcgt aagttagaaa tacttgaatg ccaaattaat tttgttttaa gaaagcttat
     6001 ttttaacata attttttagt gtggagggtt tgtaaataat gtaaagtttg taaacctgta
     6061 tttaaaatac caaagttttt cttcttttat aacattgtaa gatactagaa aaatgggctt
     6121 caagtttttt aatagctttc acagtttgtg tagttttttt tttctatttg gttaagatgt
     6181 gagttggaaa tgtattattt gaaacattta aactaatttt tgtatttaac agcagtctca
     6241 gacatttgtc ttttcaactc agttgagttg tagatatcaa atagaaatgt ccagttttgc
     6301 ctcttttaag tactgtccag ttgaaacctc ctgactcatg tactactaca caaactagaa
     6361 aatgcccatt tatttttgtt gattcaacac tccagataca gattgataca tatatactaa
     6421 tcattttatt tcatgtaaaa gttttacatc tagggtgcct atttattttt atagatgctt
     6481 tcatccagtt ataagcttat tataactttg taaatatttc agccaacagt tgtaatgttt
     6541 aaaatactta ccttcagaat gttatatttt catgtgaaat caatttcttc taattcaaaa
     6601 aaattttaag ataaactgat ttttaagttt a
//
