LOCUS       NM_001098536            3162 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript
            variant 1, mRNA.
ACCESSION   NM_001098536
VERSION     NM_001098536.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ning,F., Du,L., Li,J., Wu,T., Zhou,J., Chen,Z., Hu,X., Zhang,Y.,
            Luan,X., Xin,H., Yuan,C. and Zhang,X.
  TITLE     The deubiquitinase USP5 promotes cholangiocarcinoma progression by
            stabilizing YBX1
  JOURNAL   Life Sci 348, 122674 (2024)
   PUBMED   38692507
  REMARK    GeneRIF: The deubiquitinase USP5 promotes cholangiocarcinoma
            progression by stabilizing YBX1.
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   He,J., Chen,Y., Xu,M. and Wu,S.
  TITLE     USP5 negatively regulates the activation of NLRP3 inflammasomes and
            participates in the pathological and physiological processes of
            Sjogren's syndrome
  JOURNAL   Int Immunopharmacol 135, 112274 (2024)
   PUBMED   38772301
  REMARK    GeneRIF: USP5 negatively regulates the activation of NLRP3
            inflammasomes and participates in the pathological and
            physiological processes of Sjogren's syndrome.
REFERENCE   3  (bases 1 to 3162)
  AUTHORS   Cai,H., Ke,Z.B., Chen,J.Y., Li,X.D., Zhu,J.M., Xue,Y.T., Ruan,Z.T.,
            Wang,Z., Lin,F., Zheng,Q.S., Wei,Y., Xue,X.Y. and Xu,N.
  TITLE     Ubiquitin-specific protease 5 promotes bladder cancer progression
            through stabilizing Twist1
  JOURNAL   Oncogene 43 (10), 703-713 (2024)
   PUBMED   38218898
  REMARK    GeneRIF: Ubiquitin-specific protease 5 promotes bladder cancer
            progression through stabilizing Twist1.
REFERENCE   4  (bases 1 to 3162)
  AUTHORS   Gao,S.T., Xin,X., Wang,Z.Y., Hu,Y.Y. and Feng,Q.
  TITLE     USP5: Comprehensive insights into structure, function, biological
            and disease-related implications, and emerging therapeutic
            opportunities
  JOURNAL   Mol Cell Probes 73, 101944 (2024)
   PUBMED   38049041
  REMARK    GeneRIF: USP5: Comprehensive insights into structure, function,
            biological and disease-related implications, and emerging
            therapeutic opportunities.
            Review article
REFERENCE   5  (bases 1 to 3162)
  AUTHORS   Peng,Z.M., Han,X.J., Wang,T., Li,J.J., Yang,C.X., Tou,F.F. and
            Zhang,Z.
  TITLE     PFKP deubiquitination and stabilization by USP5 activate aerobic
            glycolysis to promote triple-negative breast cancer progression
  JOURNAL   Breast Cancer Res 26 (1), 10 (2024)
   PUBMED   38217030
  REMARK    GeneRIF: PFKP deubiquitination and stabilization by USP5 activate
            aerobic glycolysis to promote triple-negative breast cancer
            progression.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3162)
  AUTHORS   Ansari-Lari,M.A., Shen,Y., Muzny,D.M., Lee,W. and Gibbs,R.A.
  TITLE     Large-scale sequencing in human chromosome 12p13: experimental and
            computational gene structure determination
  JOURNAL   Genome Res 7 (3), 268-280 (1997)
   PUBMED   9074930
REFERENCE   7  (bases 1 to 3162)
  AUTHORS   Ansari-Lari,M.A., Muzny,D.M., Lu,J., Lu,F., Lilley,C.E., Spanos,S.,
            Malley,T. and Gibbs,R.A.
  TITLE     A gene-rich cluster between the CD4 and triosephosphate isomerase
            genes at human chromosome 12p13
  JOURNAL   Genome Res 6 (4), 314-326 (1996)
   PUBMED   8723724
REFERENCE   8  (bases 1 to 3162)
  AUTHORS   Falquet,L., Paquet,N., Frutiger,S., Hughes,G.J., Hoang-Van,K. and
            Jaton,J.C.
  TITLE     cDNA cloning of a human 100 kDa de-ubiquitinating enzyme: the 100
            kDa human de-ubiquitinase belongs to the ubiquitin C-terminal
            hydrolase family 2 (UCH2)
  JOURNAL   FEBS Lett 376 (3), 233-237 (1995)
   PUBMED   7498549
REFERENCE   9  (bases 1 to 3162)
  AUTHORS   Wilkinson,K.D., Tashayev,V.L., O'Connor,L.B., Larsen,C.N.,
            Kasperek,E. and Pickart,C.M.
  TITLE     Metabolism of the polyubiquitin degradation signal: structure,
            mechanism, and role of isopeptidase T
  JOURNAL   Biochemistry 34 (44), 14535-14546 (1995)
   PUBMED   7578059
REFERENCE   10 (bases 1 to 3162)
  AUTHORS   Falquet,L., Paquet,N., Frutiger,S., Hughes,G.J., Hoang-Van,K. and
            Jaton,J.C.
  TITLE     A human de-ubiquitinating enzyme with both isopeptidase and
            peptidase activities in vitro
  JOURNAL   FEBS Lett 359 (1), 73-77 (1995)
   PUBMED   7851534
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            U47927.1 and BC005139.2.
            
            On Jun 2, 2019 this sequence version replaced NM_001098536.1.
            
            Summary: Ubiquitin (see MIM 191339)-dependent proteolysis is a
            complex pathway of protein metabolism implicated in such diverse
            cellular functions as maintenance of chromatin structure, receptor
            function, and degradation of abnormal proteins. A late step of the
            process involves disassembly of the polyubiquitin chains on
            degraded proteins into ubiquitin monomers. USP5 disassembles
            branched polyubiquitin chains by a sequential exo mechanism,
            starting at the proximal end of the chain (Wilkinson et al., 1995
            [PubMed 7578059]).[supplied by OMIM, Mar 2010].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC005139.2, SRR1660805.55860.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000229268.13/ ENSP00000229268.8
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3080              U47927.1           23-3102
            3081-3162           BC005139.2         3081-3162
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..3162
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /note="ubiquitin specific peptidase 5"
                     /db_xref="GeneID:8078"
                     /db_xref="HGNC:HGNC:12628"
                     /db_xref="MIM:601447"
     exon            1..141
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..2607
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin specific protease 5 (isopeptidase T); ubiquitin
                     carboxyl-terminal hydrolase 5; ubiquitin-specific
                     protease-5 (ubiquitin isopeptidase T); testicular tissue
                     protein Li 218; ubiquitin thioesterase 5; deubiquitinating
                     enzyme 5; ubiquitin-specific-processing protease 5;
                     ubiquitin thiolesterase 5"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 5 isoform
                     1"
                     /protein_id="NP_001092006.1"
                     /db_xref="CCDS:CCDS41743.1"
                     /db_xref="GeneID:8078"
                     /db_xref="HGNC:HGNC:12628"
                     /db_xref="MIM:601447"
                     /translation="MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLY
                     ICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIG
                     VEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSA
                     SRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSIL
                     CGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHL
                     SHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNS
                     CYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKP
                     VPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERN
                     CRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEK
                     KRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFP
                     DYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTP
                     DEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGN
                     SGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQ
                     ALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGP
                     GKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRV
                     AS"
     exon            142..267
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            268..334
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            335..468
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            469..614
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            615..799
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            800..894
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            895..1088
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1160
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1248
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1374
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1528
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1703
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1792
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1793..1984
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2128
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            2129..2274
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            2275..2428
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            2429..2513
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     exon            2514..3162
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3142..3147
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /note="hexamer: AATAAA"
     polyA_site      3162
                     /gene="USP5"
                     /gene_synonym="ISOT"
                     /note="major polyA site"
ORIGIN      
        1 gccgtgtgtg gagaagctgc tgccggtgtc atggcggagc tgagtgagga ggcgctgctg
       61 tcagtattac cgacgatccg ggtccctaag gctggagacc gggtccacaa agacgagtgc
      121 gccttctcct tcgacacgcc ggagtctgag gggggcctct acatctgtat gaacacgttt
      181 ctgggctttg ggaaacagta tgtggagaga catttcaata agaccggcca gcgagtctac
      241 ttgcacctcc ggcggacccg gcgcccgaaa gaggaggacc ctgctacagg cactggagac
      301 ccaccccgga agaagcccac gcggctggct attggtgttg aaggcggatt tgaccttagc
      361 gaggagaagt ttgaattaga cgaggatgtg aagattgtca ttttgccaga ttacctggag
      421 attgcccggg atggactggg gggactgcct gacattgtca gagatcgggt gaccagtgca
      481 gtggaggccc tactgtcggc cgactcagcc tcccgcaagc aggaggtgca ggcatgggat
      541 ggggaagtac ggcaggtgtc taagcatgcc ttcagcctca agcagttgga caaccctgct
      601 cgaatccctc cctgtggctg gaagtgctcc aagtgtgaca tgagagagaa cctgtggctc
      661 aacctgactg atggctccat cctctgtggg cgacgctact tcgatggcag tgggggcaac
      721 aaccacgctg tggagcacta ccgagagaca ggctacccgt tagctgtcaa gctgggcacc
      781 atcacccctg atggagctga cgtgtactca tatgatgagg atgacatggt cctggacccc
      841 agcctggctg agcacctgtc ccacttcggc atcgacatgc tgaagatgca gaagacagac
      901 aagacgatga ctgagttgga gatagacatg aaccagcgga ttggtgaatg ggagctgatc
      961 caggagtcag gtgtgccact caagcccctg tttgggcctg gctacacagg catccggaac
     1021 ctgggtaaca gctgctacct caactctgtg gtccaggtgc tcttcagcat ccctgacttc
     1081 cagaggaagt atgtggataa gctggagaag atcttccaga atgccccgac ggaccctacc
     1141 caggatttca gcacccaggt ggccaagctg ggccatggcc ttctctccgg ggagtattcc
     1201 aagccagtac cggagtcggg cgatggggag cgggtgccag aacagaagga agttcaagat
     1261 ggcattgccc ctcggatgtt caaggccctc atcggcaagg gccaccctga attctccacc
     1321 aaccggcagc aggatgccca ggagttcttc cttcacctta tcaacatggt ggagaggaat
     1381 tgccggagct ctgaaaatcc taatgaagtg ttccgcttct tggtggagga aaagatcaag
     1441 tgcctggcca cagagaaggt gaagtacacc cagcgagttg actacatcat gcagctgcct
     1501 gtgcccatgg atgcagccct taacaaagag gagcttctgg agtacgagga gaagaagcgg
     1561 caagccgaag aggagaagat ggcactgcca gaactggttc gggcccaggt gcccttcagc
     1621 tcttgcctgg aggcctacgg ggcccctgag caggtcgatg acttctggag cacggccctg
     1681 caggccaagt cagtagctgt caagaccaca cgatttgcct cattccctga ctacctggtc
     1741 atccagatca agaagttcac cttcggctta gactgggtgc ccaagaaact ggatgtgtcc
     1801 atcgagatgc cagaggagct cgacatctcc cagttgaggg gcacagggct gcagcccgga
     1861 gaggaggagc tgccagacat tgccccaccc ctggtcactc cggatgagcc caaaggtagc
     1921 cttggtttct atggcaacga agacgaagac tccttctgct cccctcactt ctcctctccg
     1981 acatcgccca tgctggatga atcagtcatc atccagctgg tggagatggg attccctatg
     2041 gacgcctgcc gcaaagctgt ctactacacg ggcaacagcg gggctgaggc cgccatgaac
     2101 tgggtcatgt cacacatgga tgatccagat tttgcaaacc ccctcatcct gcctggctct
     2161 agtgggccgg gctccacaag cgcagcagcc gacccccctc ctgaggactg tgtgaccacc
     2221 attgtctcca tgggcttctc ccgggaccag gccttgaaag cgctgcgggc cacgaacaat
     2281 agtttagaac gggctgtgga ctggatcttc agtcacattg acgacctgga tgctgaagct
     2341 gccatggaca tctcagaggg ccgctcagct gccgactcca tctctgagtc tgtgccagtg
     2401 ggacctaaag tccgggatgg tcctggaaag tatcagctct ttgccttcat tagtcacatg
     2461 ggcacctcta ccatgtgtgg tcactacgtc tgccacatca agaaagaagg cagatgggtg
     2521 atctacaatg accagaaagt gtgtgcctcc gagaagccgc ccaaggacct gggctacatc
     2581 tacttctacc agagagtggc cagctaagag cctgcctcac cccttaccaa tgagggcagg
     2641 ggaagaccac ctggcatgag ggagaggggc tgagggatgg acttcagccc ctctgctctg
     2701 tacccttttt ccttttgtcc ccggcagcag ggaagaagct ggaggccgtg ggagaatggc
     2761 tgggcagagc agaggggcag cgatagactc tggggatgga gcaggacggg gacgggaggg
     2821 gccggccacc tgtctgtaag gagactttgt tgcttcccct gcccccggaa tccacagtgc
     2881 tctgcttctc tgtgtcgccc cgcccagccc cctggtgtgg agggaggggt ctcgtttgtg
     2941 cgcgtgggtg tagctttgtg catcctctcc cagtggagcg atcacctgtg cctcccctcc
     3001 ccctttgttt gcccctgtgt ggttggtcaa ggagggatgt gagggaaata gggacccccc
     3061 gacttgccct cctgcctcag tctttccccc accctgtctc ttccttgtcc ttctctggaa
     3121 aatgccaaaa tacacgatgt gaataaaagt acaacggcta aa
//
