LOCUS       NM_032236               4419 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 48 (USP48), transcript
            variant 1, mRNA.
ACCESSION   NM_032236 NM_018391 NM_032759
VERSION     NM_032236.8
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Zhang,G., Yu,J. and Wan,Y.
  TITLE     USP48 deubiquitination stabilizes SLC1A5 to inhibit retinal pigment
            epithelium cell inflammation, oxidative stress and ferroptosis in
            the progression of diabetic retinopathy
  JOURNAL   J Bioenerg Biomembr 56 (3), 311-321 (2024)
   PUBMED   38427128
  REMARK    GeneRIF: USP48 deubiquitination stabilizes SLC1A5 to inhibit
            retinal pigment epithelium cell inflammation, oxidative stress and
            ferroptosis in the progression of diabetic retinopathy.
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Chen,S., Xu,Y. and Yang,B.
  TITLE     CircUSP48 promotes malignant behavior by regulating CYR61 via
            miR-365 in osteosarcoma
  JOURNAL   Funct Integr Genomics 23 (3), 270 (2023)
   PUBMED   37553503
  REMARK    GeneRIF: CircUSP48 promotes malignant behavior by regulating CYR61
            via miR-365 in osteosarcoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4419)
  AUTHORS   Jantaree,P., Chaithongyot,S., Sokolova,O. and Naumann,M.
  TITLE     USP48 and A20 synergistically promote cell survival in Helicobacter
            pylori infection
  JOURNAL   Cell Mol Life Sci 79 (8), 461 (2022)
   PUBMED   35913642
  REMARK    GeneRIF: USP48 and A20 synergistically promote cell survival in
            Helicobacter pylori infection.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4419)
  AUTHORS   Zhang,P., Li,L., Wang,B., Ran,X., Yang,S., Luo,Y., Li,Y., Wang,Z.,
            Liu,Y. and Zhu,B.
  TITLE     miR-489-3p promotes malignant progression of non-small cell lung
            cancer through the inactivation of Wnt/beta-catenin signaling
            pathway via regulating USP48
  JOURNAL   Respir Res 23 (1), 93 (2022)
   PUBMED   35413838
  REMARK    GeneRIF: miR-489-3p promotes malignant progression of non-small
            cell lung cancer through the inactivation of Wnt/beta-catenin
            signaling pathway via regulating USP48.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4419)
  AUTHORS   Abraham,A.P., Pai,R., Beno,D.L., Chacko,G., Asha,H.S.,
            Rajaratnam,S., Kapoor,N., Thomas,N. and Chacko,A.G.
  TITLE     USP8, USP48, and BRAF mutations differ in their genotype-phenotype
            correlation in Asian Indian patients with Cushing's disease
  JOURNAL   Endocrine 75 (2), 549-559 (2022)
   PUBMED   34664215
  REMARK    GeneRIF: USP8, USP48, and BRAF mutations differ in their
            genotype-phenotype correlation in Asian Indian patients with
            Cushing's disease.
REFERENCE   6  (bases 1 to 4419)
  AUTHORS   Tzimas,C., Michailidou,G., Arsenakis,M., Kieff,E., Mosialos,G. and
            Hatzivassiliou,E.G.
  TITLE     Human ubiquitin specific protease 31 is a deubiquitinating enzyme
            implicated in activation of nuclear factor-kappaB
  JOURNAL   Cell Signal 18 (1), 83-92 (2006)
   PUBMED   16214042
  REMARK    GeneRIF: The Overexpression of USP31 in HEK 293T cells inhibited
            TNFalpha, CD40, LMP1, TRAF2, TRAF6 and IKKbeta-mediated NF-kappaB
            activation, but did not inhibit Smad-mediated transcription
            activation.
REFERENCE   7  (bases 1 to 4419)
  AUTHORS   Lockhart,P.J., Hulihan,M., Lincoln,S., Hussey,J., Skipper,L.,
            Bisceglio,G., Wilkes,K. and Farrer,M.J.
  TITLE     Identification of the human ubiquitin specific protease 31 (USP31)
            gene: structure, sequence and expression analysis
  JOURNAL   DNA Seq 15 (1), 9-14 (2004)
   PUBMED   15354349
  REMARK    GeneRIF: Northern blot analysis revealed a single USP31 transcript
            of approximately 4 kb, which was primarily expressed in the testis
            and lung.
REFERENCE   8  (bases 1 to 4419)
  AUTHORS   Quesada,V., Diaz-Perales,A., Gutierrez-Fernandez,A., Garabaya,C.,
            Cal,S. and Lopez-Otin,C.
  TITLE     Cloning and enzymatic analysis of 22 novel human ubiquitin-specific
            proteases
  JOURNAL   Biochem Biophys Res Commun 314 (1), 54-62 (2004)
   PUBMED   14715245
REFERENCE   9  (bases 1 to 4419)
  AUTHORS   Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C.
  TITLE     Human and mouse proteases: a comparative genomic approach
  JOURNAL   Nat Rev Genet 4 (7), 544-558 (2003)
   PUBMED   12838346
  REMARK    Review article
REFERENCE   10 (bases 1 to 4419)
  AUTHORS   Wilkinson,K.D.
  TITLE     Regulation of ubiquitin-dependent processes by deubiquitinating
            enzymes
  JOURNAL   FASEB J 11 (14), 1245-1256 (1997)
   PUBMED   9409543
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB134378.1, AF502942.1,
            BQ027743.1 and AL359815.26.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_032236.7.
            
            Summary: This gene encodes a protein containing domains that
            associate it with the peptidase family C19, also known as family 2
            of ubiquitin carboxyl-terminal hydrolases. Family members function
            as deubiquitinating enzymes, recognizing and hydrolyzing the
            peptide bond at the C-terminal glycine of ubiquitin. Enzymes in
            peptidase family C19 are involved in the processing of
            poly-ubiquitin precursors as well as that of ubiquitinated
            proteins. Alternate transcriptional splice variants, encoding
            different isoforms, have been characterized. [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF502942.1, BC143349.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308271.14/ ENSP00000309262.9
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                DB134378.1         51-102
            53-4122             AF502942.1         1-4070
            4123-4138           BQ027743.1         16-31               c
            4139-4419           AL359815.26        103084-103364       c
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.12"
     gene            1..4419
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /note="ubiquitin specific peptidase 48"
                     /db_xref="GeneID:84196"
                     /db_xref="HGNC:HGNC:18533"
                     /db_xref="MIM:617445"
     exon            1..326
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..3300
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /EC_number="3.4.19.12"
                     /note="isoform a is encoded by transcript variant 1;
                     ubiquitin specific protease 31; ubiquitin
                     carboxyl-terminal hydrolase 48; ubiquitin thioesterase 48;
                     deubiquitinating enzyme 48; ubiquitin-specific-processing
                     protease 48; ubiquitin thiolesterase 48"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 48 isoform
                     a"
                     /protein_id="NP_115612.4"
                     /db_xref="CCDS:CCDS30623.1"
                     /db_xref="GeneID:84196"
                     /db_xref="HGNC:HGNC:18533"
                     /db_xref="MIM:617445"
                     /translation="MAPRLQLEKAAWRWAETVRPEEVSQEHIETAYRIWLEPCIRGVC
                     RRNCKGNPNCLVGIGEHIWLGEIDENSFHNIDDPNCERRKKNSFVGLTNLGATCYVNT
                     FLQVWFLNLELRQALYLCPSTCSDYMLGDGIQEEKDYEPQTICEHLQYLFALLQNSNR
                     RYIDPSGFVKALGLDTGQQQDAQEFSKLFMSLLEDTLSKQKNPDVRNIVQQQFCGEYA
                     YVTVCNQCGRESKLLSKFYELELNIQGHKQLTDCISEFLKEEKLEGDNRYFCENCQSK
                     QNATRKIRLLSLPCTLNLQLMRFVFDRQTGHKKKLNTYIGFSEILDMEPYVEHKGGSY
                     VYELSAVLIHRGVSAYSGHYIAHVKDPQSGEWYKFNDEDIEKMEGKKLQLGIEEDLAE
                     PSKSQTRKPKCGKGTHCSRNAYMLVYRLQTQEKPNTTVQVPAFLQELVDRDNSKFEEW
                     CIEMAEMRKQSVDKGKAKHEEVKELYQRLPAGAEPYEFVSLEWLQKWLDESTPTKPID
                     NHACLCSHDKLHPDKISIMKRISEYAADIFYSRYGGGPRLTVKALCKECVVERCRILR
                     LKNQLNEDYKTVNNLLKAAVKGSDGFWVGKSSLRSWRQLALEQLDEQDGDAEQSNGKM
                     NGSTLNKDESKEERKEEEELNFNEDILCPHGELCISENERRLVSKEAWSKLQQYFPKA
                     PEFPSYKECCSQCKILEREGEENEALHKMIANEQKTSLPNLFQDKNRPCLSNWPEDTD
                     VLYIVSQFFVEEWRKFVRKPTRCSPVSSVGNSALLCPHGGLMFTFASMTKEDSKLIAL
                     IWPSEWQMIQKLFVVDHVIKITRIEVGDVNPSETQYISEPKLCPECREGLLCQQQRDL
                     REYTQATIYVHKVVDNKKVMKDSAPELNVSSSETEEDKEEAKPDGEKDPDFNQSNGGT
                     KRQKISHQNYIAYQKQVIRRSMRHRKVRGEKALLVSANQTLKELKIQIMHAFSVAPFD
                     QNLSIDGKILSDDCATLGTLGVIPESVILLKADEPIADYAAMDDVMQVCMPEEGFKGT
                     GLLGH"
     exon            327..447
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            448..604
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            605..732
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            733..857
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            858..966
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1100
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1183
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1363
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1492
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1642
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1840
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1955
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2086
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2155
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2156..2280
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2403
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..2465
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2466..2576
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2577..2707
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2708..2814
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2815..2919
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2920..3075
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            3076..3201
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            3202..3250
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            3251..3277
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            3278..4419
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3463..3468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /note="hexamer: AATAAA"
     polyA_site      3493
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /note="major polyA site"
     regulatory      3789..3794
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /note="hexamer: AATAAA"
     polyA_site      3815
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /note="major polyA site"
     regulatory      4100..4105
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /note="hexamer: AATAAA"
     polyA_site      4123
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
     regulatory      4128..4133
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /note="hexamer: AGTAAA"
     polyA_site      4157
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
     regulatory      4169..4174
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
                     /note="hexamer: TATAAA"
     polyA_site      4190
                     /gene="USP48"
                     /gene_synonym="DFNA85; RAP1GA1; USP31"
ORIGIN      
        1 gcggccggtc gtgcgggtcg ggcgcgggcg ggcgcggcgg cagtggcgcg cacaggtgat
       61 tgactggcca gctgcctgaa ggagcgccag gtcctccttg ctggcaggtg gcgaagccca
      121 ttggggcggc ggtgcagacc gcggcggcgg ctgcggcggt ctggctcggg aggcgttcct
      181 ggggccaagg ccatggcccc gcggctgcag ctggagaagg cggcctggcg ctgggcggag
      241 acggtgcggc ccgaggaggt gtcgcaggag cacatcgaga ccgcttaccg catctggctg
      301 gagccctgca ttcgcggcgt gtgcagacga aactgcaaag gaaatccgaa ttgcttggtt
      361 ggtattggtg agcatatttg gttaggagaa atagatgaaa atagttttca taacatcgat
      421 gatcccaact gtgagaggag aaaaaagaac tcatttgtgg gcctgactaa ccttggagcc
      481 acttgttatg tcaacacatt tcttcaagtg tggtttctca acttggagct tcggcaggca
      541 ctctacttat gtccaagcac ttgtagtgac tacatgctgg gagacggcat ccaagaagaa
      601 aaagattatg agcctcaaac aatttgtgag catctccagt acttgtttgc cttgttgcaa
      661 aacagtaata ggcgatacat tgatccatca ggatttgtta aagccttggg cctggacact
      721 ggacaacagc aggatgctca agaattttca aagctcttta tgtctctatt ggaagatact
      781 ttgtctaaac aaaagaatcc agatgtgcgc aatattgttc aacagcagtt ctgtggagaa
      841 tatgcctatg taactgtttg caaccagtgt ggcagagagt ctaagctttt gtcaaaattt
      901 tatgagctgg agttaaatat ccaaggccac aaacagttaa cagattgtat ctcggaattt
      961 ttgaaggaag aaaaattaga aggagacaat cgctattttt gcgagaactg tcaaagcaaa
     1021 cagaatgcaa caagaaagat tcgacttctt agccttcctt gcactctgaa cttgcagcta
     1081 atgcgttttg tctttgacag gcaaactgga cataagaaaa agctgaatac ctacattggc
     1141 ttctcagaaa ttttggatat ggagccttat gtggaacata aaggtgggtc ctacgtgtat
     1201 gaactcagcg cagtcctcat acacagagga gtgagtgctt attctggcca ctacatcgcc
     1261 cacgtgaaag atccacagtc tggtgaatgg tataagttta atgatgaaga catagaaaag
     1321 atggagggga agaaattaca actagggatt gaggaagatc tagcagaacc ttctaagtct
     1381 cagacacgta aacccaagtg tggcaaagga actcattgct ctcgaaatgc atatatgttg
     1441 gtttatagac tgcaaactca agaaaagccc aacactactg ttcaagttcc agcctttctt
     1501 caagagctgg tagatcggga taattccaaa tttgaggagt ggtgtattga aatggctgag
     1561 atgcgtaagc aaagtgtgga taaaggaaaa gcaaaacacg aagaggttaa ggagctgtac
     1621 caaaggttac ctgctggagc tgagccctat gagtttgtct ctctggaatg gctgcaaaag
     1681 tggttggatg aatcaacacc taccaaacct attgataatc acgcttgcct gtgttcccat
     1741 gacaagcttc acccggataa aatatcaatt atgaagagga tatctgaata tgcagctgac
     1801 attttctata gtagatatgg aggaggtcca agactaactg tgaaagccct gtgtaaggaa
     1861 tgtgtagtag aacgttgtcg catattgcgt ctgaagaacc aactaaatga agattataaa
     1921 actgttaata atctgctgaa agcagcagta aagggcagcg atggattttg ggtggggaag
     1981 tcctccttgc ggagttggcg ccagctagct cttgaacagc tggatgagca agatggtgat
     2041 gcagaacaaa gcaacggaaa gatgaacggt agcaccttaa ataaagatga atcaaaggaa
     2101 gaaagaaaag aagaggagga attaaatttt aatgaagata ttctgtgtcc acatggtgag
     2161 ttatgcatat ctgaaaatga aagaaggctt gtttctaaag aggcttggag caaactgcag
     2221 cagtactttc caaaggctcc tgagtttcca agttacaaag agtgctgttc acagtgcaag
     2281 attttagaaa gagaagggga agaaaatgaa gccttacata agatgattgc aaacgagcaa
     2341 aagacttctc tcccaaattt gttccaggat aaaaacagac cgtgtctcag taactggcca
     2401 gaggatacgg atgtcctcta catcgtgtct cagttctttg tagaagagtg gcggaaattt
     2461 gttagaaagc ctacaagatg cagccctgtg tcatcagttg ggaacagtgc tcttttgtgt
     2521 ccccacgggg gcctcatgtt tacatttgct tccatgacca aagaagattc taaacttata
     2581 gctctcatat ggcccagtga gtggcaaatg atacaaaagc tctttgttgt ggatcatgta
     2641 attaaaatca cgagaattga agtgggagat gtaaaccctt cagaaacaca gtatatttct
     2701 gagcccaaac tctgtccaga atgcagagaa ggcttattgt gtcagcagca gagggacctg
     2761 cgtgaataca ctcaagccac catctatgtc cataaagttg tggataataa aaaggtgatg
     2821 aaggattcgg ctccggaact gaatgtgagt agttctgaaa cagaggagga caaggaagaa
     2881 gctaaaccag atggagaaaa agatccagat tttaatcaaa gcaatggtgg aacaaagcgg
     2941 caaaagatat cccatcaaaa ttatatagcc tatcaaaagc aagttattcg ccgaagtatg
     3001 cgacatagaa aagttcgtgg tgagaaagca cttctcgttt ctgctaatca gacgttaaaa
     3061 gaattgaaaa ttcagatcat gcatgcattt tcagttgctc cttttgacca gaatttgtca
     3121 attgatggaa agattttaag tgatgactgt gccaccctag gcacccttgg cgtcattcct
     3181 gaatctgtca ttttattgaa ggctgatgaa ccaattgcag attatgctgc aatggatgat
     3241 gtcatgcaag tttgtatgcc agaagaaggg tttaaaggta ctggtcttct tggacattaa
     3301 tctttgaata cttgctgact gctaagaaat gaccagaggg gaagaggagt ttgacatgtt
     3361 agggcattaa agcaaaggtg gatttaagaa ttaaaccatt acatgcccct tccaaaaggc
     3421 agaaatccat tcaaacgtga ctgtcccaaa tgccttatgt caaataaagc agattgcact
     3481 gatggacatc agacttgaag gaaatgtttc caattttata tttaaggggg gtggtgggtg
     3541 ggagggggca agtaaagacg gaacaagttt agtagcagta atagtaaatc atgtttacat
     3601 atgagattta tagtcgtggg aggggaataa agttctgtta tatttccttg ctcgagtttc
     3661 ataccagatg cgttggtcca taaaggattg tatcaagtag atgggacaac attctgctct
     3721 gaacgaaaag taattttaga gacataacct gcttaccaat gcctgtcttt gattcatatt
     3781 ctactttcaa taaagcatga aagtgaagaa cttgtcctaa gtgtggaaaa gtgtcttcag
     3841 atttagactc ttctccatgt cagctgcagc gccacccgcc ttacacctgc ccggccgtct
     3901 gtctcttggt attgggtaaa ggagggggca cctgcatgtc tcctgcaatg agcaaggaat
     3961 tatgtctcat gttttgactt cagaggcttt ttgctttggt gcatttcaga aaggatggag
     4021 aacatttatt atgtgtgaaa gcatcctctt ccggttttgc tgttattcaa aagtgggaaa
     4081 tgtacctggc acgtttgaaa ataaaaaatc tgactaccta tcagaagagt aaatcagact
     4141 gaagtacatt tggataacac aaggtttcta taaaatttgt tcttcctgtc ctccatgtca
     4201 ctgtttcttg gacctcagtt ctctttttga aagcattatt ccaaaatgcc ctgagagggt
     4261 ctcttagatc attgtttaaa aaaggaaaaa agtatatgga tgtgctgtcc atccaactca
     4321 ggattatcat tcttagcaac acgtaaccga agcaatattc ttaagaatat tgaaggggtt
     4381 tttttaattg aacttaagac tggagttttt cctttgaaa
//
