LOCUS       NM_001282659            9994 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 47 (USP47), transcript
            variant 1, mRNA.
ACCESSION   NM_001282659 XM_005252998
VERSION     NM_001282659.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Lei,H., Xu,H., Yang,L., Wang,Y., Zhang,Y. and Wu,Y.
  TITLE     USP47 stabilizes YBX1 to promote the progression of acute myeloid
            leukemia
  JOURNAL   Oncogene 43 (7), 539-542 (2024)
   PUBMED   38104157
  REMARK    GeneRIF: USP47 stabilizes YBX1 to promote the progression of acute
            myeloid leukemia.
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   Wang,A., Huang,H., Shi,J.H., Yu,X., Ding,R., Zhang,Y., Han,Q.,
            Ni,Z.Y., Li,X., Zhao,R. and Zou,Q.
  TITLE     USP47 inhibits m6A-dependent c-Myc translation to maintain
            regulatory T cell metabolic and functional homeostasis
  JOURNAL   J Clin Invest 133 (23), e169365 (2023)
   PUBMED   37788092
  REMARK    GeneRIF: USP47 inhibits m6A-dependent c-Myc translation to maintain
            regulatory T cell metabolic and functional homeostasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9994)
  AUTHORS   Shin,S.C., Park,J., Kim,K.H., Yoon,J.M., Cho,J., Ha,B.H., Oh,Y.,
            Choo,H., Song,E.J. and Kim,E.E.
  TITLE     Structural and functional characterization of USP47 reveals a hot
            spot for inhibitor design
  JOURNAL   Commun Biol 6 (1), 970 (2023)
   PUBMED   37740002
  REMARK    GeneRIF: Structural and functional characterization of USP47
            reveals a hot spot for inhibitor design.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9994)
  AUTHORS   Chen,H.Y., Tang,R.C., Liang,J.W., Zhao,W., Yu,S.S., Yao,R.R.,
            Xu,R., Zhang,A., Geng,S., Sun,X.Y., Ge,Q. and Zhang,J.
  TITLE     Deubiquitinase USP47 attenuates virus-induced type I interferon
            signaling
  JOURNAL   Int Immunopharmacol 118, 110040 (2023)
   PUBMED   37001379
  REMARK    GeneRIF: Deubiquitinase USP47 attenuates virus-induced type I
            interferon signaling.
REFERENCE   5  (bases 1 to 9994)
  AUTHORS   Kassel,S., Hanson,A.J., Benchabane,H., Saito-Diaz,K., Cabel,C.R.,
            Goldsmith,L., Taha,M., Kanuganti,A., Ng,V.H., Xu,G., Ye,F.,
            Picker,J., Port,F., Boutros,M., Weiss,V.L., Robbins,D.J.,
            Thorne,C.A., Ahmed,Y. and Lee,E.
  TITLE     USP47 deubiquitylates Groucho/TLE to promote Wnt-beta-catenin
            signaling
  JOURNAL   Sci Signal 16 (771), eabn8372 (2023)
   PUBMED   36749823
  REMARK    GeneRIF: USP47 deubiquitylates Groucho/TLE to promote
            Wnt-beta-catenin signaling.
REFERENCE   6  (bases 1 to 9994)
  AUTHORS   Yang,S.W., Oh,K.H., Park,E., Chang,H.M., Park,J.M., Seong,M.W.,
            Ka,S.H., Song,W.K., Park,D.E., Baas,P.W., Jeon,Y.J. and Chung,C.H.
  TITLE     USP47 and C terminus of Hsp70-interacting protein (CHIP)
            antagonistically regulate katanin-p60-mediated axonal growth
  JOURNAL   J Neurosci 33 (31), 12728-12738 (2013)
   PUBMED   23904609
  REMARK    GeneRIF: The results of this study indicated that USP47 plays a
            crucial role in the control of axonal growth during neuronal
            development by antagonizing CHIP-mediated katanin-p60 degradation.
REFERENCE   7  (bases 1 to 9994)
  AUTHORS   Parsons,J.L., Dianova,I.I., Khoronenkova,S.V., Edelmann,M.J.,
            Kessler,B.M. and Dianov,G.L.
  TITLE     USP47 is a deubiquitylating enzyme that regulates base excision
            repair by controlling steady-state levels of DNA polymerase beta
  JOURNAL   Mol Cell 41 (5), 609-615 (2011)
   PUBMED   21362556
  REMARK    GeneRIF: USP47 has a role in regulating DNA repair and maintaining
            genome integrity
REFERENCE   8  (bases 1 to 9994)
  AUTHORS   Peschiaroli,A., Skaar,J.R., Pagano,M. and Melino,G.
  TITLE     The ubiquitin-specific protease USP47 is a novel beta-TRCP
            interactor regulating cell survival
  JOURNAL   Oncogene 29 (9), 1384-1393 (2010)
   PUBMED   19966869
  REMARK    GeneRIF: USP47, a novel beta-Trcp interactor, regulates cell growth
            and survival, potentially providing a novel target for anticancer
            therapies.
REFERENCE   9  (bases 1 to 9994)
  AUTHORS   Quesada,V., Diaz-Perales,A., Gutierrez-Fernandez,A., Garabaya,C.,
            Cal,S. and Lopez-Otin,C.
  TITLE     Cloning and enzymatic analysis of 22 novel human ubiquitin-specific
            proteases
  JOURNAL   Biochem Biophys Res Commun 314 (1), 54-62 (2004)
   PUBMED   14715245
REFERENCE   10 (bases 1 to 9994)
  AUTHORS   Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C.
  TITLE     Human and mouse proteases: a comparative genomic approach
  JOURNAL   Nat Rev Genet 4 (7), 544-558 (2003)
   PUBMED   12838346
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104383.4 and AC124276.5.
            
            On Aug 12, 2019 this sequence version replaced NM_001282659.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.34955.1, BC071559.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000527733.7/ ENSP00000433146.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-253               AC104383.4         64816-65068
            254-457             AC104383.4         103021-103224
            458-571             AC104383.4         107311-107424
            572-710             AC104383.4         114812-114950
            711-807             AC124276.5         194177-194273       c
            808-953             AC124276.5         189010-189155       c
            954-1033            AC124276.5         188528-188607       c
            1034-1183           AC124276.5         186322-186471       c
            1184-1279           AC124276.5         171619-171714       c
            1280-1432           AC124276.5         171376-171528       c
            1433-1600           AC124276.5         168979-169146       c
            1601-1732           AC124276.5         162305-162436       c
            1733-1809           AC124276.5         161750-161826       c
            1810-1865           AC124276.5         161119-161174       c
            1866-1978           AC124276.5         158754-158866       c
            1979-2083           AC124276.5         157935-158039       c
            2084-2291           AC124276.5         155360-155567       c
            2292-2407           AC124276.5         153498-153613       c
            2408-2527           AC124276.5         151322-151441       c
            2528-3305           AC124276.5         148758-149535       c
            3306-3481           AC124276.5         143750-143925       c
            3482-3562           AC124276.5         143312-143392       c
            3563-3678           AC124276.5         141866-141981       c
            3679-3797           AC124276.5         141388-141506       c
            3798-3928           AC124276.5         138999-139129       c
            3929-3976           AC124276.5         136926-136973       c
            3977-4107           AC124276.5         136706-136836       c
            4108-9994           AC124276.5         129983-135869       c
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.3"
     gene            1..9994
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /note="ubiquitin specific peptidase 47"
                     /db_xref="GeneID:55031"
                     /db_xref="HGNC:HGNC:20076"
                     /db_xref="MIM:614460"
     exon            1..253
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     CDS             215..4282
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /EC_number="3.4.19.12"
                     /note="isoform a is encoded by transcript variant 1;
                     ubiquitin carboxyl-terminal hydrolase 47; ubiquitin
                     thioesterase 47; deubiquitinating enzyme 47;
                     ubiquitin-specific-processing protease 47; ubiquitin
                     thiolesterase 47; Trf (TATA binding protein-related
                     factor)-proximal homolog"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 47 isoform
                     a"
                     /protein_id="NP_001269588.1"
                     /db_xref="CCDS:CCDS60725.1"
                     /db_xref="GeneID:55031"
                     /db_xref="HGNC:HGNC:20076"
                     /db_xref="MIM:614460"
                     /translation="MVPGEENQLVPKEIENAAEEPRVLCIIQDTTNSKTVNERITLNL
                     PASTPVRKLFEDVANKVGYINGTFDLVWGNGINTADMAPLDHTSDKSLLDANFEPGKK
                     NFLHLTDKDGEQPQILLEDSSAGEDSVHDRFIGPLPREGSGGSTSDYVSQSYSYSSIL
                     NKSETGYVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQR
                     LFVLLQTSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADL
                     INELYQGKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPE
                     ILDGPNQYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFP
                     EELDMSTFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEGICLETNS
                     GTEKISKSGLEKNSLIYELFSVMVHSGSAAGGHYYACIKSFSDEQWYSFNDQHVSRIT
                     QEDIKKTHGGSSGSRGYYSSAFASSTNAYMLIYRLKDPARNAKFLEVDEYPEHIKNLV
                     QKERELEEQEKRQREIERNTCKIKLFCLHPTKQVMMENKLEVHKDKTLKEAVEMAYKM
                     MDLEEVIPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKP
                     DQVFQSYKPGEVMVKVHVVDLKAESVAAPITVRAYLNQTVTEFKQLISKAIHLPAETM
                     RIVLERCYNDLRLLSVSSKTLKAEGFFRSNKVFVESSETLDYQMAFADSHLWKLLDRH
                     ANTIRLFVLLPEQSPVSYSKRTAYQKAGGDSGNVDDDCERVKGPVGSLKSVEAILEES
                     TEKLKSLSLQQQQDGDNGDSSKSTETSDFENIESPLNERDSSASVDNRELEQHIQTSD
                     PENFQSEERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADNAQIPLANGLDSHSITS
                     SRRTKANEGKKETWDTAEEDSGTDSEYDESGKSRGEMQYMYFKAEPYAADEGSGEGHK
                     WLMVHVDKRITLAAFKQHLEPFVGVLSSHFKVFRVYASNQEFESVRLNETLSSFSDDN
                     KITIRLGRALKKGEYRVKVYQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQ
                     CGLELSIDRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQ
                     LAVLSRRWKPSEMKLDPFQEVVLESSSVDELREKLSEISGIPLDDIEFAKGRGTFPCD
                     ISVLDIHQDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELMELTDEQRNELMKKES
                     SRLQKTGHRVTYSPRKEKALKIYLDGAPNKDLTQD"
     exon            254..457
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            458..571
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            572..710
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            711..807
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            808..953
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1033
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1183
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1279
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1432
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1600
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1732
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1809
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..1865
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1978
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2083
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2291
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2407
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2527
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            2528..3305
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            3306..3481
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            3482..3562
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            3563..3678
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            3679..3797
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            3798..3928
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            3929..3976
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            3977..4107
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     exon            4108..9994
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4656..4661
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /note="hexamer: AATAAA"
     polyA_site      4677
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /note="major polyA site"
     regulatory      5023..5028
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /note="hexamer: AATAAA"
     polyA_site      5052
                     /gene="USP47"
                     /gene_synonym="TRFP"
     regulatory      5603..5608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /note="hexamer: AATAAA"
     polyA_site      5622
                     /gene="USP47"
                     /gene_synonym="TRFP"
     regulatory      7238..7243
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /note="hexamer: AATAAA"
     polyA_site      7261
                     /gene="USP47"
                     /gene_synonym="TRFP"
     regulatory      7412..7417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP47"
                     /gene_synonym="TRFP"
                     /note="hexamer: AATAAA"
     polyA_site      7432
                     /gene="USP47"
                     /gene_synonym="TRFP"
ORIGIN      
        1 gacgaaggcg gctgtggtag cggcggcggc ggcggcggag ccctgggtcg gtgtctgcgc
       61 gctggtgtct gaggcccagg ctgaggcctc cgctattgct ggagcgcagg cggcggagag
      121 gatgactgcc gctgccattc tctcttgagc tagcgagccg ccgccaccct ccaccctccc
      181 ccggcagggc ggagaggagc ggccggagtc agcgatggtg cccggcgagg agaaccaact
      241 ggtcccgaaa gagatagaaa atgctgctga agaacctaga gtcttatgta ttatacaaga
      301 tactactaat tcaaagacag tgaatgaacg gatcacttta aatttaccag catctactcc
      361 agtcagaaag ctctttgaag atgtggccaa caaagtaggc tacataaatg gaacctttga
      421 cttggtgtgg ggaaatggaa tcaatactgc tgatatggca ccactggatc ataccagtga
      481 caagtcactt ctcgacgcta attttgagcc aggaaagaag aactttctgc atttgacaga
      541 taaagatggt gaacaacctc aaatactgct ggaggattcc agtgctgggg aagacagtgt
      601 tcatgacagg tttataggtc cgcttccaag agaaggttct gggggttcta ccagtgatta
      661 tgtcagccaa agctactcct actcatctat tttgaataaa tcagaaactg gatatgtggg
      721 actagtaaac caagcaatga cttgctattt gaatagcctt ttgcaaacac tttttatgac
      781 tcctgaattt aggaatgcat tatataagtg ggaatttgaa gaatctgaag aagatccagt
      841 gacaagtatt ccataccaac ttcaaaggct ttttgttttg ttacaaacca gcaaaaagag
      901 agcaattgaa accacagatg ttacaaggag ctttggatgg gatagtagtg aggcttggca
      961 gcagcatgat gtacaagaac tatgcagagt catgtttgat gctttggaac agaaatggaa
     1021 gcaaacagaa caggctgatc ttataaatga gctatatcaa ggcaagctga aggactacgt
     1081 gagatgtctg gaatgtggtt atgagggctg gcgaatcgac acatatcttg atattccatt
     1141 ggtcatccga ccttatgggt ccagccaagc atttgctagt gtggaagaag cattgcatgc
     1201 atttattcag ccagagattc tggatggccc aaatcagtat ttttgtgaac gttgtaagaa
     1261 gaagtgtgat gcacggaagg gccttcggtt tttgcatttt ccttatctgc tgaccttaca
     1321 gctgaaaaga ttcgattttg attatacaac catgcatagg attaaactga atgatcgaat
     1381 gacatttccc gaggaactag atatgagtac ttttattgat gttgaagatg agaaatctcc
     1441 tcagactgaa agttgcactg acagtggagc agaaaatgaa ggtagttgtc acagtgatca
     1501 gatgagcaac gatttctcca atgatgatgg tgttgatgaa ggaatctgtc ttgaaaccaa
     1561 tagtggaact gaaaagatct caaaatctgg acttgaaaag aattccttga tctatgaact
     1621 tttctctgtt atggttcatt ctgggagcgc tgctggtggt cattattatg catgtataaa
     1681 gtcattcagt gatgagcagt ggtacagctt caatgatcaa catgtcagca ggataacaca
     1741 agaggacatt aagaaaacac atggtggatc ttcaggaagc agaggatatt attctagtgc
     1801 tttcgcaagt tccacaaatg catatatgct gatctataga ctgaaggatc cagccagaaa
     1861 tgcaaaattt ctagaagtgg atgaataccc agaacatatt aaaaacttgg tgcagaaaga
     1921 gagagagttg gaagaacaag aaaagagaca acgagaaatt gagcgcaata catgcaagat
     1981 aaaattattc tgtttgcatc ctacaaaaca agtaatgatg gaaaataaat tggaggttca
     2041 taaggataag acattaaagg aagcagtaga aatggcttat aagatgatgg atttagaaga
     2101 ggtaataccc ctggattgct gtcgccttgt taaatatgat gagtttcatg attatctaga
     2161 acggtcatat gaaggagaag aagatacacc aatggggctt ctactaggtg gcgtcaagtc
     2221 aacatatatg tttgatctgc tgttggagac gagaaagcct gatcaggttt tccaatctta
     2281 taaacctgga gaagtgatgg tgaaagttca tgttgttgat ctaaaggcag aatctgtagc
     2341 tgctcctata actgttcgtg cttacttaaa tcagacagtt acagaattca aacaactgat
     2401 ttcaaaggcc atccatttac ctgctgaaac aatgagaata gtgctggaac gctgctacaa
     2461 tgatttgcgt cttctcagtg tctccagtaa aaccctgaaa gctgaaggat tttttagaag
     2521 taacaaggtg tttgttgaaa gctccgagac tttggattac cagatggcct ttgcagactc
     2581 tcatttatgg aaactcctgg atcggcatgc aaatacaatc agattatttg ttttgctacc
     2641 tgaacaatcc ccagtatctt attccaaaag gacagcatac cagaaagctg gaggcgattc
     2701 tggtaatgtg gatgatgact gtgaaagagt caaaggacct gtaggaagcc taaagtctgt
     2761 ggaagctatt ctagaagaaa gcactgaaaa actcaaaagc ttgtcactgc agcaacagca
     2821 ggatggagat aatggggaca gcagcaaaag tactgagaca agtgactttg aaaacatcga
     2881 atcacctctc aatgagaggg actcttcagc atcagtggat aatagagaac ttgaacagca
     2941 tattcagact tctgatccag aaaattttca gtctgaagaa cgatcagact cagatgtgaa
     3001 taatgacagg agtacaagtt cagtggacag tgatattctt agctccagtc atagcagtga
     3061 tactttgtgc aatgcagaca atgctcagat ccctttggct aatggacttg actctcacag
     3121 tatcacaagt agtagaagaa cgaaagcaaa tgaagggaaa aaagaaacat gggatacagc
     3181 agaagaagac tctggaactg atagtgaata tgatgagagt ggcaagagta ggggagaaat
     3241 gcagtacatg tatttcaaag ctgaacctta tgctgcagat gaaggttctg gggaaggaca
     3301 taaatggttg atggtgcatg ttgataaaag aattactctg gcagctttca aacaacattt
     3361 agagcccttt gttggagttt tgtcctctca cttcaaggtc tttcgagtgt atgccagcaa
     3421 tcaagagttt gagagcgtcc ggctgaatga gacactttca tcattttctg atgacaataa
     3481 gattacaatt agactgggga gagcacttaa aaaaggagaa tacagagtta aagtatacca
     3541 gcttttggtc aatgaacaag agccatgcaa gtttctgcta gatgctgtgt ttgctaaagg
     3601 aatgactgta cggcaatcaa aagaggaatt aattcctcag ctcagggagc aatgtggttt
     3661 agagctcagt attgacaggt ttcgtctaag gaaaaaaaca tggaagaatc ctggcactgt
     3721 ctttttggat tatcatattt atgaagaaga tattaatatt tccagcaact gggaggtttt
     3781 ccttgaagtt cttgatgggg tagagaagat gaagtccatg tcacagcttg cagttttgtc
     3841 aagacggtgg aagccttcag agatgaagtt ggatcccttc caggaggttg tattggaaag
     3901 cagtagtgtg gacgaattgc gagagaagct tagtgaaatc agtgggattc ctttggatga
     3961 tattgaattt gctaagggta gaggaacatt tccctgtgat atttctgtcc ttgatattca
     4021 tcaggattta gactggaatc ctaaagtttc taccctgaat gtctggcctc tttatatctg
     4081 tgatgatggt gcggtcatat tttataggga taaaacagaa gaattaatgg aattgacaga
     4141 tgagcaaaga aatgaactga tgaaaaaaga aagcagtcga ctccagaaga ctggacatcg
     4201 tgtaacatac tcacctcgta aagagaaagc actaaaaata tatctggatg gagcaccaaa
     4261 taaagatctg actcaagact gactctgata gtgtagcatt ttccctgggg gagttttggt
     4321 tttaattaga tggttcacta ccactgggta gtgccatttt ggccggacat ggttggggta
     4381 acccagtgac accagcactg attggactgc cctacaccaa tcagaagctc agtgcccaat
     4441 gggccactgt tttgactcgg aatcatgttg tgcactatag tcaaatgtac tgtaaagtga
     4501 aaagggatgt gcaaaaaaat aaaaaaaaac aacaaaaaaa gctaaccttc tattagaaaa
     4561 ggggacaggg gaatgagtaa acttctttta ttgcggacaa atgtgcacat agccgctagt
     4621 aaaactagcc tcaaacagga tgctcatagc ttaataataa aagctgtgca aaggccatga
     4681 atgaatgaat tttctgttta tttcactgat gcacacatta cctcattgac aattcagaag
     4741 taaatccaac gtgtgttgac tcttggaaag cagcaaaaac aggagctgaa gaaaagaaat
     4801 tcttggaacc agccgtaacc cagtaaggaa ttgtgaagtt gtgtttttat tttgtttcat
     4861 tttttgcaga gtattaagaa cattattctg gaacatcaga acgtttccct tagaccgatc
     4921 ccagcaggtg gcagctcaga ttgctgcagt gttgtaatta taactgattg tacttaagtt
     4981 atggatgtag agaatatgtt tcattcattt attcagcatg taaataaaat tgatcctgtt
     5041 gagttatcat aattgcagtt caactatctg ccatgattat tcttttcacg tatcattcat
     5101 tctgtacatt tgtgtacatt gagaagtata gcaatctatg taaatgtaat cctcagtgag
     5161 gttcctcagt gctaggtccc ataggattgt cgttgccctt gttaatgagg tttctctgtt
     5221 cagcggcttc aatttttttc tctttgtaca tctagttttg aagatttact tcaagtttga
     5281 atcttctaga atgcttgtaa gtccagtttt aatttttaga gtcaatttgt agttacatgt
     5341 agtttaactt ttgggaaacg tcttaacatt gttctgaata aacttgctaa tgaggtcagg
     5401 tcatggtaca gactgatgca gtcaacatga tttcattgca gagtttatta gtatcagcaa
     5461 gtttttgctt tgctaaataa aagtactcaa tgaacacaat tctacataaa ttttgacata
     5521 ccatctaatt tataaaaatc aataaaaaag gttttggtaa aactttttca tgccagatgc
     5581 tgtttacaac aatgaacatg ccaataaaac atttgttcat tctgttgtgt tattttagtc
     5641 attaaacttc tgtggatgaa gaatctgggt taagaataga tttgtcatct ttaaatatga
     5701 cattttgtaa tgtgtattgg atatctcatt tctatgataa aggtatattt acagtaaagt
     5761 tctcataaga gaaatgaaaa gctgtgttaa tatctaactt tggggaaccc tgtcagtatt
     5821 tcagatccga tttttaccct ttttttctta taagaaagat aaaattagaa aatactgtta
     5881 gcaaatgtgg ctctgccatt tgaatataat caccgagaat tccatgtctt aaaagtctcc
     5941 tggaatccac aatgaaaaaa aaaatctttt ctaaggtatt tttctggcta atttttattt
     6001 gaagaaagct atagcattta gcgaaatttg actgaagtaa tgttctgagt ttgcattagt
     6061 gggattggtg atgttctcag aagaaaattg gaaacacttg tgatgaattg tctttcagat
     6121 cacttagatt ttctgatgta agaggacagc tgtttggttc tgatacaggc ctgcttactt
     6181 gggatgtagg gttagtaaat ggggtttctg ctttaaagga ctgacttgct atcacacaaa
     6241 agaggcagac ttgtaaacac aatgggcttt ggagtttggt ctgattgggt ttggtttagt
     6301 attcctatga gcgtaaatgg taaaattctt ctgataccca ctctttagac tgtgccttct
     6361 gctctgttct ttgttttatg tttaactgct gtttctaatt gcaggtgtat tacagataca
     6421 aataagagta aagaaaatat atttcattat agaaaagaaa aaattaaaag cttcttgctt
     6481 ttcagtgcct gatagagtga aaacacaaag ttgcacttta ataatttcaa taaaagctaa
     6541 tctgtgtcag cctccctctg cttcagagag tcaggtgagc atccataacc taacaggcag
     6601 agccctagcg atgtggatca agtttcctga gcccgggggc ggtggagcct catgatctct
     6661 tatcttttga ggctgaggca ggtcacatgc aacaaattgt gaccctgctc cccacaagtc
     6721 atgcaaaggt tttgaagagc ttttaccgtg gggcagatga acttgtgtca accatgcaca
     6781 ccctgtgaga accaagtacc tgtgtttcta aggcgggcac tcaaggtgag gggtgcattc
     6841 tggccaaaga aacaaaagct gtggtttcag gaccatgccg tgtgtagctg atctgtacgg
     6901 gacgtgtatg taaggaagag caatcatgat agataagaac agtgtgtgaa gcagccttca
     6961 cactagagtg tttggtcatc tcttataatg taagggaagg tactttaaaa ttctgggaag
     7021 atgcgatgaa ctcatgtccc agtcagaaaa taatccaatg aaataagcat tggttgccag
     7081 gccacagtta ggaattgtat tgtgatacat ctagaggcca agagagcagg agagagctac
     7141 caacttacac tgtggtttaa gctaaatgac cgcacagcat catagcattg cagtgttgtt
     7201 actaaatctg gaagtgacct gtgaatgtat ggaatacaat aaagtctttt attctggttc
     7261 atttgctagt acttcctttt tgattggata ctgtagttct tcctctggat tttattttgt
     7321 tcagcgtcaa ggccctaatt ttgcaaatgt agtctaaacc acattacgtg gactagagga
     7381 tactctgaat tagcaagttt tttgtttgct gaataaaact attccatctt aacaattctg
     7441 tgtaattttt gaaaaacatc aacattttgg tcactagtag tcaaaatctt ataaggccat
     7501 atgtcctgaa aggcaaaact ctgtgtatgt gcgatttacc agggagatag taggacagtt
     7561 tttatgaggg tttcaaagga gttcatggcc caaatgaagc ttgtggccac ctaggtttag
     7621 tgtacatgaa attggaagat gcttcttgat tgtagttccc atggggaaga gggaggctgt
     7681 gatcatttgg ttagatacag cattaattgt cacaacttga ctttcagaaa caagaatact
     7741 gcacaaactc aagcatgcat tggagcatgt gttactattt agaaaagagt gcagccatca
     7801 gtaggtcaag ctaggagggc aaaagagagt tgagaggaat gctctgcccc ttccccatca
     7861 cagcactgcc cctttccacc tcctcaccag ctcacctgta caagagaatt gatctcaact
     7921 gagtgtcagc tcaccaggtc agaaattgtt attggaccct aaaaccttgc ctcacaggtg
     7981 ggcaccaagc tccccttccc tctgctttcc atgtttccca cctgccctct ccccacctgt
     8041 tttcagcctc ttttataatg cttaagtaac ctgcttgaag agtataattt atggagataa
     8101 aaatactcaa tgcttacatt tttttcataa ctgtgccaat tgttttgtca aatgcagtta
     8161 cccactgggc agtgggctta tcaagtgctc agtgcaatcc tgtgactttt aagtcatttt
     8221 gcattattaa gacaatctta tcttaaagtt cagtggtttc attcaaagct cccctgaacc
     8281 tccatagtgc ctccaagatg tgctgtccct gccaactcct gcaccagctt gactggtggt
     8341 ggaagaggcc aggaagagcg gggggaagac atgtgctaac cacttcttag caatgaggct
     8401 ggtaaggtta ctgtcccctt gtgcctggtc actagtaact tttttgtaga catgaaggta
     8461 gatgccctgc aggtcctatc agcaaaggac ccaactccta acagcattgg tgctccctca
     8521 tgaaatttga cagtgtcttg ccagcctcct gctggcagac acttctctgc agtgaatacc
     8581 actatcccat gctgcactag tccctaccaa gtcttcaacc ctcaatttga tcttccatga
     8641 gtgtctcaaa aagttcccct cagaagtttc agccaaggga ataaaatcta acctctcctc
     8701 cctcaacgga gtgtcaggga ggggaagaat ctgttttcca aacaccacaa tctcccaaaa
     8761 ggggatttca ccctcccttg gtcatcttta tacagggcaa ttgtgctgct gctgctgagg
     8821 tggccacttt cagcaagccc tgtgtgcagg ggcctagctg catttagaaa ccaagcatag
     8881 ctatcaaaaa gtaatctcca aatagttgaa ttgcatttct tgagtatctg gagaggatgt
     8941 gcagaccaca ggaataccta atgccttttt tctcttcctg tctttgtccc tcacactaca
     9001 gcaggcccct cccttccctc ttcaacctca tcctccctcc ccacaggccc agagaaccag
     9061 ttgggctttg ttctcctgca ggctatggtt catcatgcaa atagctcctg tgtcagaaat
     9121 gctttttggc ttcaaataac agaaaagcta acaccagctt tatcaataat aatatcggtg
     9181 gtttacttaa ggtgtccaga gatggtggag aacaggattg gtttcctcct caatgtcaag
     9241 gactcaaaga ctctttctgt ggtagggcca catcctaaac cctgtatcct gtgattattt
     9301 acctgacagg gcaaaagaga ttttgcagat gcaattaagg ttaaggacct tgacgtggga
     9361 agattgtgat tatttacctg acagggcaaa agagattttg cagatgcaat taaggttaag
     9421 gaccttgacg tgggaagatt attctggatt atctaggtgg gcgcaatttg atcacatggg
     9481 tccccagaag tggagaacct ttcccacctg tagaaagcca gagagctggc acctgagaag
     9541 gacagaactg tcattgcagg atttgaagat gaaggggccc atgagccaag gaatgccagt
     9601 gacctataga ggctaaaaaa cagcaaggaa atggactctc cccagagcct ccagaggaat
     9661 gcagccctgt tgatcacatg atcaccagat ggctgcccca gagccaaatg tcgcttcctg
     9721 agcaccatac tcaaaggcag gggaagtgga tggagggcag gagctccatt cttgtttgcc
     9781 actctccttt tgtcaattgg gaaaaaattc cagaaactct gggagccctc cccttacatt
     9841 tcctgggtca tggggccagc cctagctgct ggagggactg agaacagctg ttgagcagtt
     9901 tacctgacgg catctgccat ggcttggcag gaactctggc tttgggagag agcagcagca
     9961 aggtattcaa gcaccacctc cacccagccc ctcc
//
