LOCUS       NM_001346022            5878 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 45 (USP45), transcript
            variant 3, mRNA.
ACCESSION   NM_001346022 XM_005267169
VERSION     NM_001346022.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Liu,L., Chen,C., Liu,P., Li,J., Pang,Z., Zhu,J., Lin,Z., Zhou,H.,
            Xie,Y., Lan,T., Chen,Z.S., Zeng,Z. and Fang,W.
  TITLE     MYH10 Combines with MYH9 to Recruit USP45 by Deubiquitinating Snail
            and Promotes Serous Ovarian Cancer Carcinogenesis, Progression, and
            Cisplatin Resistance
  JOURNAL   Adv Sci (Weinh) 10 (14), e2203423 (2023)
   PUBMED   36929633
  REMARK    GeneRIF: MYH10 Combines with MYH9 to Recruit USP45 by
            Deubiquitinating Snail and Promotes Serous Ovarian Cancer
            Carcinogenesis, Progression, and Cisplatin Resistance.
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Yi,Z., Ouyang,J., Sun,W., Xiao,X., Li,S., Jia,X., Wang,P. and
            Zhang,Q.
  TITLE     Biallelic mutations in USP45, encoding a deubiquitinating enzyme,
            are associated with Leber congenital amaurosis
  JOURNAL   J Med Genet 56 (5), 325-331 (2019)
   PUBMED   30573563
  REMARK    GeneRIF: Our study implicates that biallelic mutations in USP45 are
            associated with the occurrence of LCA. Moreover, our results
            indicate that USP45 is indispensable to the maintenance of
            photoreceptor function.
REFERENCE   3  (bases 1 to 5878)
  AUTHORS   Conte,C., Griffis,E.R., Hickson,I. and Perez-Oliva,A.B.
  TITLE     USP45 and Spindly are part of the same complex implicated in cell
            migration
  JOURNAL   Sci Rep 8 (1), 14375 (2018)
   PUBMED   30258100
  REMARK    GeneRIF: Using mass spectrometry this study identified CCDC99 as a
            new target of USP45. The data showed that CCDC99 and USP45 are part
            of the same complex and that their interaction specifically depends
            on the catalytic activity of USP45.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5878)
  AUTHORS   Perez-Oliva,A.B., Lachaud,C., Szyniarowski,P., Munoz,I.,
            Macartney,T., Hickson,I., Rouse,J. and Alessi,D.R.
  TITLE     USP45 deubiquitylase controls ERCC1-XPF endonuclease-mediated DNA
            damage responses
  JOURNAL   EMBO J 34 (3), 326-343 (2015)
   PUBMED   25538220
  REMARK    GeneRIF: these results establish USP45 as a new regulator of
            XPF-ERCC1 crucial for efficient DNA repair
REFERENCE   5  (bases 1 to 5878)
  AUTHORS   Quesada,V., Diaz-Perales,A., Gutierrez-Fernandez,A., Garabaya,C.,
            Cal,S. and Lopez-Otin,C.
  TITLE     Cloning and enzymatic analysis of 22 novel human ubiquitin-specific
            proteases
  JOURNAL   Biochem Biophys Res Commun 314 (1), 54-62 (2004)
   PUBMED   14715245
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL137784.14 and AL513550.9.
            
            On Feb 3, 2020 this sequence version replaced NM_001346022.2.
            
            Summary: The protein encoded by this gene is a deubiquitylase that
            binds ERCC1, the catalytic subunit of the XPF-ERCC1 DNA repair
            endonuclease. This endonuclease is a critical regulator of DNA
            repair processes, and the deubiquitylase activity of the encoded
            protein is important for maintaining the DNA repair ability of
            XPF-ERCC1. [provided by RefSeq, Sep 2016].
            
            Transcript Variant: This variant (3), as well as variants 1 and 2,
            encodes isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.3258052.1,
                                           SRR14038195.537705.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000500704.7/ ENSP00000424372.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                AL137784.14        32909-32940         c
            33-142              AL137784.14        27638-27747         c
            143-315             AL137784.14        26127-26299         c
            316-419             AL137784.14        24945-25048         c
            420-520             AL137784.14        21282-21382         c
            521-660             AL137784.14        6198-6337           c
            661-756             AL137784.14        5717-5812           c
            757-887             AL137784.14        270-400             c
            888-975             AL513550.9         121465-121552       c
            976-1057            AL513550.9         113859-113940       c
            1058-1149           AL513550.9         111994-112085       c
            1150-1206           AL513550.9         110402-110458       c
            1207-1350           AL513550.9         109926-110069       c
            1351-2017           AL513550.9         91119-91785         c
            2018-2115           AL513550.9         88887-88984         c
            2116-2202           AL513550.9         85091-85177         c
            2203-2356           AL513550.9         82568-82721         c
            2357-5878           AL513550.9         77647-81168         c
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q16.2"
     gene            1..5878
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /note="ubiquitin specific peptidase 45"
                     /db_xref="GeneID:85015"
                     /db_xref="HGNC:HGNC:20080"
                     /db_xref="MIM:618439"
     exon            1..32
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            33..142
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..2487
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /EC_number="3.4.19.12"
                     /note="isoform a is encoded by transcript variant 3;
                     ubiquitin specific protease 45; ubiquitin
                     carboxyl-terminal hydrolase 45; ubiquitin thioesterase 45;
                     deubiquitinating enzyme 45; ubiquitin-specific-processing
                     protease 45; ubiquitin thiolesterase 45"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 45 isoform
                     a"
                     /protein_id="NP_001332951.1"
                     /db_xref="CCDS:CCDS34501.1"
                     /db_xref="GeneID:85015"
                     /db_xref="HGNC:HGNC:20080"
                     /db_xref="MIM:618439"
                     /translation="MRVKDPTKALPEKAKRSKRPTVPHDEDSSDDIAVGLTCQHVSHA
                     ISVNHVKRAIAENLWSVCSECLKERRFYDGQLVLTSDIWLCLKCGFQGCGKNSESQHS
                     LKHFKSSRTEPHCIIINLSTWIIWCYECDEKLSTHCNKKVLAQIVDFLQKHASKTQTS
                     AFSRIMKLCEEKCETDEIQKGGKCRNLSVRGITNLGNTCFFNAVMQNLAQTYTLTDLM
                     NEIKESSTKLKIFPSSDSQLDPLVVELSRPGPLTSALFLFLHSMKETEKGPLSPKVLF
                     NQLCQKAPRFKDFQQQDSQELLHYLLDAVRTEETKRIQASILKAFNNPTTKTADDETR
                     KKVKAYGKEGVKMNFIDRIFIGELTSTVMCEECANISTVKDPFIDISLPIIEERVSKP
                     LLWGRMNKYRSLRETDHDRYSGNVTIENIHQPRAAKKHSSSKDKSQLIHDRKCIRKLS
                     SGETVTYQKNENLEMNGDSLMFASLMNSESRLNESPTDDSEKEASHSESNVDADSEPS
                     ESESASKQTGLFRSSSGSGVQPDGPLYPLSAGKLLYTKETDSGDKEMAEAISELRLSS
                     TVTGDQDFDRENQPLNISNNLCFLEGKHLRSYSPQNAFQTLSQSYITTSKECSIQSCL
                     YQFTSMELLMGNNKLLCENCTKNKQKYQEETSFAEKKVEGVYTNARKQLLISAVPAVL
                     ILHLKRFHQAGLSLRKVNRHVDFPLMLDLAPFCSATCKNASVGDKVLYGLYGIVEHSG
                     SMREGHYTAYVKVRTPSRKLSEHNTKKKNVPGLKAADNESAGQWVHVSDTYLQVVPES
                     RALSAQAYLLFYERVL"
     exon            143..315
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            316..419
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            420..520
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            521..660
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            661..756
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            757..887
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            888..975
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1057
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1149
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1206
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1350
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..2017
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2115
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2202
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2356
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     exon            2357..5878
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5737..5742
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /note="hexamer: AATAAA"
     polyA_site      5764
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /note="major polyA site"
     regulatory      5860..5865
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP45"
                     /gene_synonym="LCA19"
                     /note="hexamer: AATAAA"
     polyA_site      5878
                     /gene="USP45"
                     /gene_synonym="LCA19"
ORIGIN      
        1 aggttgtaga cgctgcggcc cggcccggcg ggtaaataac agatgcgggt gaaagatcca
       61 actaaagctt tacctgagaa agccaaaaga agtaaaaggc ctactgtacc tcatgatgaa
      121 gactcttcag atgatattgc tgtaggttta acttgccaac atgtaagtca tgctatcagc
      181 gtgaatcatg taaagagagc aatagctgag aatctgtggt cagtttgctc agaatgttta
      241 aaagaaagaa gattctatga tgggcagcta gtacttactt ctgatatttg gttgtgcctc
      301 aagtgtggct tccagggatg tggtaaaaac tcagaaagcc aacattcatt gaagcacttt
      361 aagagttcca gaacagagcc ccattgtatt ataattaatc tgagcacatg gattatatgg
      421 tgttatgaat gtgatgaaaa attatcaacg cattgtaata agaaggtttt ggctcagata
      481 gttgattttc tccagaaaca tgcttctaaa acacaaacaa gtgcattttc tagaatcatg
      541 aaactttgtg aagaaaaatg tgaaacagat gaaatacaga agggaggaaa atgcagaaat
      601 ttatctgtaa gaggaattac aaatttagga aatacttgct tttttaatgc agtcatgcag
      661 aacttggcac agacttatac tcttactgat ctgatgaatg agatcaaaga aagtagtaca
      721 aaactcaaga tttttccttc ctcagactct cagctggacc cattggtggt ggaactttca
      781 aggcctggac cactgacctc agccttgttc ctgtttcttc acagcatgaa ggagactgaa
      841 aaaggaccac tttctcctaa agttcttttt aatcagcttt gtcagaaggc acctcgattt
      901 aaagatttcc agcaacagga cagtcaggag cttcttcatt atcttctgga tgcagtgagg
      961 acagaagaaa caaagcgaat acaagctagc attctaaaag catttaacaa cccaactact
     1021 aaaactgctg atgatgaaac tagaaaaaaa gtcaaagcat atggaaaaga aggtgtgaaa
     1081 atgaacttca tagatcggat ctttattggt gaattaacta gcacggtcat gtgtgaagaa
     1141 tgtgcaaata tctccacggt gaaagatcca ttcattgata tttcacttcc tataatagaa
     1201 gaaagggttt caaaaccttt actttgggga agaatgaata aatatagaag tttacgggag
     1261 acagatcatg atcgatacag tggcaatgtt actatagaaa atattcatca acctagagct
     1321 gccaagaagc attcttcatc taaagataag agtcaactaa ttcatgaccg aaaatgtatt
     1381 agaaaattgt catctggaga aactgtcaca taccagaaaa atgaaaacct tgaaatgaat
     1441 ggggattctt taatgtttgc cagcctcatg aattctgagt cacgtctgaa tgaaagccct
     1501 actgatgaca gtgaaaaaga agccagccat tctgaaagca atgttgatgc tgacagtgag
     1561 ccttcagaat ctgaaagtgc ttcaaagcag actgggctgt tcagatccag tagtggatcc
     1621 ggtgtgcagc cagatggacc cctttaccct ctgtcagcag gtaaactgct gtacaccaag
     1681 gagactgaca gtggtgataa ggaaatggca gaagctattt ctgaacttcg tttgagcagc
     1741 actgtaactg gagatcaaga ttttgacaga gaaaatcagc cactaaatat ttcaaataat
     1801 ttatgttttt tagaggggaa gcatttgagg tcttatagtc cccaaaatgc ttttcagacc
     1861 ctttctcaga gctatataac tacttctaaa gaatgttcaa ttcagtcctg tctctaccag
     1921 tttacatcta tggaattact aatggggaat aataagcttc tatgtgagaa ttgtactaaa
     1981 aacaaacaga agtaccaaga agaaaccagt tttgcagaaa agaaagtaga aggagtttat
     2041 actaatgcca ggaagcaatt gctcatttct gctgttccag ctgtcctaat tctccacctg
     2101 aaaagatttc atcaggctgg cttgagtctt cgtaaagtaa acagacatgt agattttcca
     2161 cttatgctcg atttagcacc attctgctct gctacttgta agaatgcaag tgtgggagat
     2221 aaagttctct acggtctcta tggcatagtg gaacatagtg gctcgatgag agaaggccac
     2281 tacactgctt atgtgaaagt gagaacaccc tccaggaaat tatcggaaca taacactaaa
     2341 aagaaaaatg tgcctggttt gaaagcggct gataatgaat cagcaggcca gtgggtccat
     2401 gttagtgaca cttacttaca ggtggttcca gaatcaagag cacttagtgc acaagcctac
     2461 cttcttttct atgaaagagt attataacta ttaatggtaa tgattattta ggtcatttgt
     2521 ttttgaatgc cacagtgata actataatat ataatgtgcc tttctagtct tccctcttct
     2581 gtaggaatag catgttcctc aaatggtcct gaactttttc accattttgg tgaacccttt
     2641 taaagtaaat ttactcaatg ctttaaaatt catagtctta aaataaatgt gaattttgtt
     2701 tccaggtatt tattctgggg tacaaaaact tcccagaatt tacagtagga aaggaaaccc
     2761 ctttatgatg tggcttatta ttacaagcat tcagaaatga tgctggctaa gtcaaatcat
     2821 tccttgagac agtgattcct aaatgtaatg ccaccttcct gaactctcac atattctata
     2881 tcatggttat tttaaaaaat atatttttag ccttttgtaa ccttagtctt gttttgagca
     2941 atcatattcc gtgttttatg tgcttttata tttttataat aaatattaaa gtaaaactat
     3001 taaaaattgt ttatccacgt accacttaac ttaaaatcat ctcagggcca tctagtggta
     3061 tatatgtgta ctaccattta ggaaactgct ataacacata atttcatgaa gtaacaccta
     3121 atacggtgta gttccctggt catattttat acaattcaac catataaaag ggtgtcactg
     3181 taatttcagt agtgtgggtt tacaaataat ctgctggtta gcttattacc ttgaggtttt
     3241 gaaaaactag aattatattg aggcatttca taaacatatc tcttgcaccc tcttcatggt
     3301 ggagttaagg ataacttgca ggtggttggc caaggcccaa tatagatgat tataacattt
     3361 agaattggca attagaagtt gataatccat ataggaccat aggatagctt tgaaatataa
     3421 attaccaaat tatatttgta aatgatagag aattatattc ttaacagaaa aatatatact
     3481 ttttctactt cttgtaaata ctaaacacag ttttggggtt ttgaaatcac tttgtcttca
     3541 gcaagcatca ttaataagtt caggtgaatt tagcttttaa tggggcatta agctggcatt
     3601 tttgattttc tttttaaata atagcaaaaa tatcattttt cccctctaga atacaaatta
     3661 ttgagctgtc aaaacttggc atgaagaata tactattact taggcttaaa tatcaaaaga
     3721 taatggctac ttcagtgtca tattgttgaa atagaaacaa aactgcatgg ttcaagcatc
     3781 ctaatcatta gtgccaatga atgatagtgc aggcaagtgc caggtaaatg cctagtccca
     3841 cttcattctc ctcctgggaa gaaaggataa gaatagtggc tgcagataag gtatggaaat
     3901 atttaatcaa gactgtctta aacacataag catatataat gttatcaagg ttaccagtgc
     3961 tgccttttga tatatggatg aacataactg taattaaaga cggtaatagt atacaaaatg
     4021 cagagcttaa aaatgtcatt gcataaattc tatttttaag atgttgaaat gttagtagtg
     4081 tttttcttga tttttaacag tgaaataatc aagaaatata tctttaagtt aatctgcaaa
     4141 gtcaggctgt gttctgtaaa aggtatttca cagcattttc tgtttaactg taatctgtta
     4201 tcatggagat ctcagtacca gagcagtgaa ttttttgaaa aatattgact gtctttattc
     4261 aagtcagcca tcttggtaag agcagttttc atccccccac tgcccaaatg taaatataga
     4321 tcacctaatt tttcttacag aaagatactg ttaagaggca tgcacttttc atcggatggt
     4381 cacttgtttc ttgtttcaaa gacaaatatt tggatagata cccatacttg ctatgagtca
     4441 gtcacagtgc tgacactatt ttaacatcat ttaaaatgga aagatctaca tagatttttg
     4501 acattattcc agaagtaaca tgcaagtaag atatttttat tgtcattttc ttcatgttaa
     4561 aggagctact tatgaaacct atttttttat ggcctagagt ttatgagtgc tttttccttg
     4621 tcttctgaaa atggaataaa ttggattttt gatacttaag aatttcctaa agatagcttt
     4681 tgtaacagag aaagcatgtt aaattacaca tgggtctacc ttaaaatttt tgttattatt
     4741 tccaaagcac atttgcaggg atagtaaatg tatatagtag caagtctact ataataacag
     4801 tagtttctgg atcacctcag gaaacagtat aaataaataa taatgtttac ttatcatcaa
     4861 cataactacc atagtgtttg aatgaattaa agtttcatta gctttattta gaaactggtt
     4921 taccccagct tcccgttttt ttccaaatat aaaatgataa aatattgatt atgtgccaat
     4981 attgtcattt aatgttctta aaacttgtga agaccacctt tttaggtagc tgagaagagt
     5041 gatagttggt atcaatttgt tagccaggca tggtggctca tgctgttggg aggctgagtg
     5101 gggaagatgc ttgagcccag gaggtcaagg tgcagtgaac catgatcaca ccactgcatt
     5161 cctgcagagt gacggagtga gaccctgtct caaaaataaa taaaaagaag acagttggta
     5221 tcaattcaaa gaatagttga tgcctgttct cttattttaa atgtaccaac agagctattt
     5281 tactgtattt aatagttctt agcttccatg tgtgatgatt ttaatcagtg attttatgca
     5341 atagctgaag aaaattttct gaagattaag ctgtgaaatg tatcaaccct catctaattt
     5401 ttgtagtaca aaatgacatc ctgattcctt ttaaaaattc acgtttgtaa tttatttttc
     5461 tgcatgtatt gatcaaataa gcctaaacca aatatgtgca ataaaattga gcctttagat
     5521 agtatctagt gcattgttaa aaaagattat ttctagatat ttatgatgtt tttgtttacg
     5581 tagaaatcat gaattcaaat gtttaaaaga tgaccatatt ttcaattttg agtaatacat
     5641 attattccta ctgttttttt tcttttgcca tgtttctaga aaacagctgt gtaacttatt
     5701 gagcgttatt ttggtgttac tgtataaaac atgcataata aatattctta tttatctcat
     5761 gttacaagat tggtcctgga attttcacag tttacgttat tgacagaatg ttttatcaac
     5821 aatgcatttg cttatgtctt agtccactca ggctgctata ataaaatacc atcaacta
//
