LOCUS       NM_032147               4008 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 44 (USP44), transcript
            variant 1, mRNA.
ACCESSION   NM_032147
VERSION     NM_032147.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ekstrom,T.L., Hussain,S., Bedekovics,T., Ali,A., Paolini,L.,
            Mahmood,H., Rosok,R.M., Koster,J., Johnsen,S.A. and Galardy,P.J.
  TITLE     USP44 Overexpression Drives a MYC-Like Gene Expression Program in
            Neuroblastoma through Epigenetic Reprogramming
  JOURNAL   Mol Cancer Res 22 (9), 812-825 (2024)
   PUBMED   38775808
  REMARK    GeneRIF: USP44 Overexpression Drives a MYC-Like Gene Expression
            Program in Neuroblastoma through Epigenetic Reprogramming.
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Zhang,B., Sun,R., Gu,M., Jiang,Z., Wang,Y., Zhang,L., Liu,X. and
            Chi,Z.
  TITLE     RNA-binding protein NOVA1 promotes acute T-lymphocyte leukemia
            progression by stabilizing USP44 mRNA
  JOURNAL   Biochem Cell Biol 102 (1), 60-72 (2024)
   PUBMED   37816258
  REMARK    GeneRIF: RNA-binding protein NOVA1 promotes acute T-lymphocyte
            leukemia progression by stabilizing USP44 mRNA.
REFERENCE   3  (bases 1 to 4008)
  AUTHORS   Jiang,W., Yang,X., Shi,K., Zhang,Y., Shi,X., Wang,J., Wang,Y.,
            Chenyan,A., Shan,J., Wang,Y., Chang,J., Chen,R., Zhou,T., Zhu,Y.,
            Yu,Y., Li,C. and Li,X.
  TITLE     MAD2 activates IGF1R/PI3K/AKT pathway and promotes
            cholangiocarcinoma progression by interfering USP44/LIMA1 complex
  JOURNAL   Oncogene 42 (45), 3344-3357 (2023)
   PUBMED   37752233
  REMARK    GeneRIF: MAD2 activates IGF1R/PI3K/AKT pathway and promotes
            cholangiocarcinoma progression by interfering USP44/LIMA1 complex.
REFERENCE   4  (bases 1 to 4008)
  AUTHORS   Zhou,H., Yang,L., Lin,X., Chan,T.F., Lee,N.P., Tse,W.K.F.,
            Zhang,X., Li,R. and Lai,K.P.
  TITLE     Integrated network findings reveal ubiquitin-specific protease 44
            overexpression suppresses tumorigenicity of liver cancer
  JOURNAL   Aging (Albany NY) 15 (10), 4304-4318 (2023)
   PUBMED   37204480
  REMARK    GeneRIF: Integrated network findings reveal ubiquitin-specific
            protease 44 overexpression suppresses tumorigenicity of liver
            cancer.
REFERENCE   5  (bases 1 to 4008)
  AUTHORS   Chi,Z., Zhang,B., Sun,R., Wang,Y., Zhang,L. and Xu,G.
  TITLE     USP44 accelerates the growth of T-cell acute lymphoblastic leukemia
            through interacting with WDR5 and repressing its ubiquitination
  JOURNAL   Int J Med Sci 19 (14), 2022-2032 (2022)
   PUBMED   36483601
  REMARK    GeneRIF: USP44 accelerates the growth of T-cell acute lymphoblastic
            leukemia through interacting with WDR5 and repressing its
            ubiquitination.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4008)
  AUTHORS   Suresh,B., Ramakrishna,S., Lee,H.J., Choi,J.H., Kim,J.Y., Ahn,W.S.
            and Baek,K.H.
  TITLE     K48- and K63-linked polyubiquitination of deubiquitinating enzyme
            USP44
  JOURNAL   Cell Biol Int 34 (8), 799-808 (2010)
   PUBMED   20402667
REFERENCE   7  (bases 1 to 4008)
  AUTHORS   Sowa,M.E., Bennett,E.J., Gygi,S.P. and Harper,J.W.
  TITLE     Defining the human deubiquitinating enzyme interaction landscape
  JOURNAL   Cell 138 (2), 389-403 (2009)
   PUBMED   19615732
REFERENCE   8  (bases 1 to 4008)
  AUTHORS   Stegmeier,F., Rape,M., Draviam,V.M., Nalepa,G., Sowa,M.E.,
            Ang,X.L., McDonald,E.R. 3rd, Li,M.Z., Hannon,G.J., Sorger,P.K.,
            Kirschner,M.W., Harper,J.W. and Elledge,S.J.
  TITLE     Anaphase initiation is regulated by antagonistic ubiquitination and
            deubiquitination activities
  JOURNAL   Nature 446 (7138), 876-881 (2007)
   PUBMED   17443180
  REMARK    GeneRIF: a dynamic balance of ubiquitination by the APC and
            deubiquitination by USP44 contributes to the generation of the
            switch-like transition controlling anaphase entry
REFERENCE   9  (bases 1 to 4008)
  AUTHORS   Quesada,V., Diaz-Perales,A., Gutierrez-Fernandez,A., Garabaya,C.,
            Cal,S. and Lopez-Otin,C.
  TITLE     Cloning and enzymatic analysis of 22 novel human ubiquitin-specific
            proteases
  JOURNAL   Biochem Biophys Res Commun 314 (1), 54-62 (2004)
   PUBMED   14715245
REFERENCE   10 (bases 1 to 4008)
  AUTHORS   Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C.
  TITLE     Human and mouse proteases: a comparative genomic approach
  JOURNAL   Nat Rev Genet 4 (7), 544-558 (2003)
   PUBMED   12838346
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB443014.1, DB456039.1,
            DA092275.1, AK315697.1, AC018475.32 and BM023146.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_032147.4.
            
            Summary: The protein encoded by this gene is a protease that
            functions as a deubiquitinating enzyme. The encoded protein is
            thought to help regulate the spindle assembly checkpoint by
            preventing early anaphase onset. This protein specifically
            deubiquitinates CDC20, which stabilizes the anaphase promoting
            complex/cyclosome. [provided by RefSeq, Dec 2016].
            
            Transcript Variant: This variant (1) encodes the same protein as
            variants 2, 3, 5, and 6.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL136825.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2151119, SAMEA2161674
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000258499.8/ ENSP00000258499.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-62                DB443014.1         12-73
            63-402              DB456039.1         30-369
            403-748             DA092275.1         229-574
            749-2414            AK315697.1         766-2431
            2415-3739           AC018475.32        47137-48461
            3740-4008           BM023146.1         14-282              c
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q22"
     gene            1..4008
                     /gene="USP44"
                     /note="ubiquitin specific peptidase 44"
                     /db_xref="GeneID:84101"
                     /db_xref="HGNC:HGNC:20064"
                     /db_xref="MIM:610993"
     exon            1..205
                     /gene="USP44"
                     /inference="alignment:Splign:2.1.0"
     exon            206..1703
                     /gene="USP44"
                     /inference="alignment:Splign:2.1.0"
     CDS             276..2414
                     /gene="USP44"
                     /EC_number="3.4.19.12"
                     /note="ubiquitin specific protease 44;
                     ubiquitin-specific-processing protease 44;
                     deubiquitinating enzyme 44; ubiquitin thioesterase 44;
                     ubiquitin thiolesterase 44"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 44"
                     /protein_id="NP_115523.2"
                     /db_xref="CCDS:CCDS9053.1"
                     /db_xref="GeneID:84101"
                     /db_xref="HGNC:HGNC:20064"
                     /db_xref="MIM:610993"
                     /translation="MLAMDTCKHVGQLQLAQDHSSLNPQKWHCVDCNTTESIWACLSC
                     SHVACGRYIEEHALKHFQESSHPVALEVNEMYVFCYLCDDYVLNDNTTGDLKLLRRTL
                     SAIKSQNYHCTTRSGRFLRSMGTGDDSYFLHDGAQSLLQSEDQLYTALWHRRRILMGK
                     IFRTWFEQSPIGRKKQEEPFQEKIVVKREVKKRRQELEYQVKAELESMPPRKSLRLQG
                     LAQSTIIEIVSVQVPAQTPASPAKDKVLSTSENEISQKVSDSSVKRRPIVTPGVTGLR
                     NLGNTCYMNSVLQVLSHLLIFRQCFLKLDLNQWLAMTASEKTRSCKHPPVTDTVVYQM
                     NECQEKDTGFVCSRQSSLSSGLSGGASKGRKMELIQPKEPTSQYISLCHELHTLFQVM
                     WSGKWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQRELETTGTSLPALI
                     PTSQRKLIKQVLNVVNNIFHGQLLSQVTCLACDNKSNTIEPFWDLSLEFPERYQCSGK
                     DIASQPCLVTEMLAKFTETEALEGKIYVCDQCNSKRRRFSSKPVVLTEAQKQLMICHL
                     PQVLRLHLKRFRWSGRNNREKIGVHVGFEEILNMEPYCCRETLKSLRPECFIYDLSAV
                     VMHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMDEVCKAQAYILFYTQRVTEN
                     GHSKLLPPELLLGSQHPNEDADTSSNEILS"
     exon            1704..1899
                     /gene="USP44"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2008
                     /gene="USP44"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2214
                     /gene="USP44"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..4008
                     /gene="USP44"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3983..3988
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP44"
                     /note="hexamer: ATTAAA"
     polyA_site      4008
                     /gene="USP44"
                     /note="major polyA site"
ORIGIN      
        1 agtttccagg aggtgagagt ttaagccaaa cctgaaagag gttgatttgt aatgatttgc
       61 aggggggcac tggaggcagc cgccaggact tttcacttag gagatcagca tttgccctga
      121 tggaaactgg gcgatcctgc agggactgac ctctgagtta tccaaaggcc gacctgggga
      181 aagactgatt ttgaggtttt aatagttttc agatgcttca agtgttgtga acagagactt
      241 gtttggatta tgcatttctc agctagacta aataaatgct agcaatggat acgtgcaaac
      301 atgttgggca gctgcagctt gctcaagacc attccagcct caaccctcag aaatggcact
      361 gtgtggactg caacacgacc gagtccattt gggcttgcct tagctgctcc catgttgcct
      421 gtggaagata tattgaagag catgcactca agcactttca agaaagcagt catcctgttg
      481 cattggaggt gaatgagatg tacgtttttt gttacctttg tgatgattat gttctgaatg
      541 ataacacaac tggagacctg aagttactac gacgtacatt aagtgccatc aaaagtcaaa
      601 attatcactg cacaactcgt agtgggaggt ttttacggtc catgggtaca ggtgatgatt
      661 cttatttctt acatgacggt gcccaatctc tgcttcaaag tgaagatcaa ctgtatactg
      721 ctctttggca caggagaagg atactaatgg gtaaaatctt tcgaacatgg tttgaacaat
      781 cacccattgg aagaaaaaag caagaagaac catttcagga aaaaatagta gtaaaaagag
      841 aagtaaagaa aagacggcag gaattggagt atcaagttaa agcagaattg gaaagtatgc
      901 ctccaagaaa gagtttacgt ttacaagggc tcgctcagtc gaccataata gaaatagttt
      961 ctgttcaggt gccagcacaa acgccagcat caccagcaaa agataaagta ctctctacct
     1021 cagaaaatga aatatctcaa aaagtcagtg actcctcagt taaacgaagg ccaatagtaa
     1081 ctcctggtgt aacaggattg agaaatttgg gaaatacttg ctatatgaat tctgttcttc
     1141 aggtgttgag tcatttactt atttttcgac aatgtttttt aaagcttgat ctgaaccaat
     1201 ggctggctat gactgctagc gagaagacaa gatcttgtaa gcatccacca gtcacagata
     1261 cagtagtata tcaaatgaat gaatgtcagg aaaaagatac aggttttgtt tgctccagac
     1321 aatcaagtct gtcatcagga ctaagtggtg gagcatcaaa aggtagaaag atggaactta
     1381 ttcagccaaa ggagccaact tcacagtaca tttctctttg tcatgaattg catactttgt
     1441 tccaagtcat gtggtctgga aagtgggcgt tggtctcacc atttgctatg ctacactcag
     1501 tgtggagact cattcctgcc tttcgtggtt acgcccaaca agacgctcag gaatttcttt
     1561 gtgaactttt agataaaata caacgtgaat tagagacaac tggtaccagt ttaccagctc
     1621 ttatccccac ttctcaaagg aaactcatca aacaagttct gaatgttgta aataacattt
     1681 ttcatggaca acttcttagt caggttacat gtcttgcatg tgacaacaaa tcaaatacca
     1741 tagaaccttt ctgggacttg tcattggagt ttccagaaag gtatcaatgc agtggaaaag
     1801 atattgcttc ccagccatgt ctggttactg aaatgttggc caaatttaca gaaactgaag
     1861 ctttagaagg aaaaatctac gtatgtgacc agtgtaactc aaagcgtaga aggttttcct
     1921 ccaaaccagt tgtactcaca gaagcccaga aacaacttat gatatgccac ctacctcagg
     1981 ttctcagact gcacctcaaa cgattcaggt ggtcaggacg taataaccga gagaagattg
     2041 gtgttcatgt tggctttgag gaaatcttaa acatggagcc ctattgctgc agggagaccc
     2101 tgaaatccct cagaccagaa tgctttatct atgacttgtc cgcggtggtg atgcaccatg
     2161 ggaaaggatt tggctcaggg cactacactg cctactgcta taattctgaa ggagggttct
     2221 gggtacactg caatgattcc aaactaagca tgtgcactat ggatgaagta tgcaaggctc
     2281 aagcttatat cttgttttat acccaacgag ttactgagaa tggacattct aaacttttgc
     2341 ctccagagct cctgttgggg agccaacatc ccaatgaaga cgctgatacc tcgtctaatg
     2401 aaatccttag ctgatccaaa gacaatgggg ttttcttcct gtgatttata tatatacttt
     2461 ttaaaagact gatgtaccat tttaaacttc attttttctt gtgaatcagt gtatactaca
     2521 tttatacatt ttatatctaa caattttttt ttttacaaag tataaatgta tatatcaact
     2581 gaaggtaact acttttttca tatttggagt tttaaacttt tggtgtttac ctcagactga
     2641 tgttacctct tttatatttt tatgtcttaa ttggctcgga tgatgaactt gtgcaatctt
     2701 ctaccaacaa agttcaagtg gcatcatttt atatacatgt atctttttca ggtattttct
     2761 atacaaattc ttaatagatg gaaaattaga ctctactttg gtcactaata gtctttcatt
     2821 tgtatattga agttaccttg ccccttggag ttattgaagt gacatgtcaa ggtatcacct
     2881 aaatattctt cagtcacact cactggtatt tctgaggctt tgtgtgttaa caggccttgt
     2941 aattgacatt attttggtta atgtaacccc aaaattgctt tagtaattgc tctttggcat
     3001 agtcaaacta taaatgaaaa tggcagcttt acaaatagta tatttaagtg aactctggaa
     3061 ctatggacat gaaaaaaatg atggctggga tttatgattt ttgtctggca gcaaacaggt
     3121 ttgtccagaa gtctaataat taagcagtca taaaaagtct gaatttagta aaccagtgta
     3181 tgatgttatt caaatagttt accttgggta tgagttcatt ttataatgtc tgatgacatt
     3241 agatctctta aaactttatg tatttttttt agttcaaagg aatagagtct tgaagagaaa
     3301 aaattatagg gcagaaaaga taagtgttca aaattggcaa ctggactatt attatgtcta
     3361 gcatctcatt ctaaataact aaagcttgat ttactcttgc taggattatg tgactactag
     3421 gtaggagcct cttaaaacac tggccctgag cattaaaaaa aaaaaaaaaa actaaaagct
     3481 atctatctaa acttgcacaa aaaaaaatac agtgtgagtc actctttttc ttcttctgat
     3541 aatctcactg ggtttcttta aagtcctgtt gctgctaact ttatcttatt tggagagggt
     3601 agaatcaact aatctctgta gaccagctat gtatctaagc tctgttaacc acaggaagat
     3661 catgtatccc ttttttataa gtgggtttat ttgcaggttg aagtcttagt gaaagatagt
     3721 atcctgtaga atgctgttca ccactagtga tcatctgtta agtacaaact tgaaagaggt
     3781 ctctaccaat aaatttttct taaatttcac aaaacaagtc atctgggtta gctgatagtg
     3841 aggtgcagtg agacttttat cactgaatag acaaatcatt tgtgccgaaa atacatctgg
     3901 aatattaaat ggctatgttt aaaattgttc ataataccta tgcaagttgt tacctcaact
     3961 gttacacact tcttaatacc tcattaaaaa tttacatggt caagataa
//
