LOCUS       NM_201624               4327 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 33 (USP33), transcript
            variant 2, mRNA.
ACCESSION   NM_201624
VERSION     NM_201624.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Chen,J., Shan,W., Jia,Q., Chen,Y., Jiang,W., Tian,Y., Huang,X.,
            Li,X., Wang,Z. and Xia,B.
  TITLE     USP33 facilitates the ovarian cancer progression via
            deubiquitinating and stabilizing CBX2
  JOURNAL   Oncogene 43 (43), 3170-3183 (2024)
   PUBMED   39256572
  REMARK    GeneRIF: USP33 facilitates the ovarian cancer progression via
            deubiquitinating and stabilizing CBX2.
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Zhong,B., Zhu,Q., Wang,L., Wang,F., Zheng,Y., Lin,S. and Cheng,X.
  TITLE     USP33 enhances cell survival and stemness by deubiquitinating
            CTNNB1 in BXPC-3 and SW1990 cells
  JOURNAL   Cell Biol Int 47 (8), 1368-1380 (2023)
   PUBMED   37076992
  REMARK    GeneRIF: USP33 enhances cell survival and stemness by
            deubiquitinating CTNNB1 in BXPC-3 and SW1990 cells.
REFERENCE   3  (bases 1 to 4327)
  AUTHORS   Zhang,A., Huang,Z., Tao,W., Zhai,K., Wu,Q., Rich,J.N., Zhou,W. and
            Bao,S.
  TITLE     USP33 deubiquitinates and stabilizes HIF-2alpha to promote hypoxia
            response in glioma stem cells
  JOURNAL   EMBO J 41 (7), e109187 (2022)
   PUBMED   35191554
  REMARK    GeneRIF: USP33 deubiquitinates and stabilizes HIF-2alpha to promote
            hypoxia response in glioma stem cells.
REFERENCE   4  (bases 1 to 4327)
  AUTHORS   Wang,H., Liu,Z., Sun,Z., Zhou,D., Mao,H. and Deng,G.
  TITLE     Ubiquitin specific peptidase 33 promotes cell proliferation and
            reduces apoptosis through regulation of the SP1/PI3K/AKT pathway in
            retinoblastoma
  JOURNAL   Cell Cycle 20 (19), 2066-2076 (2021)
   PUBMED   34470581
  REMARK    GeneRIF: Ubiquitin specific peptidase 33 promotes cell
            proliferation and reduces apoptosis through regulation of the
            SP1/PI3K/AKT pathway in retinoblastoma.
REFERENCE   5  (bases 1 to 4327)
  AUTHORS   Mishra,R. and Banerjea,A.C.
  TITLE     SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal miR-148a to
            Activate Human Microglia
  JOURNAL   Front Immunol 12, 656700 (2021)
   PUBMED   33936086
  REMARK    GeneRIF: SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal
            miR-148a to Activate Human Microglia.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4327)
  AUTHORS   Thorne,C., Eccles,R.L., Coulson,J.M., Urbe,S. and Clague,M.J.
  TITLE     Isoform-specific localization of the deubiquitinase USP33 to the
            Golgi apparatus
  JOURNAL   Traffic 12 (11), 1563-1574 (2011)
   PUBMED   21801292
REFERENCE   7  (bases 1 to 4327)
  AUTHORS   Curcio-Morelli,C., Zavacki,A.M., Christofollete,M., Gereben,B., de
            Freitas,B.C., Harney,J.W., Li,Z., Wu,G. and Bianco,A.C.
  TITLE     Deubiquitination of type 2 iodothyronine deiodinase by von
            Hippel-Lindau protein-interacting deubiquitinating enzymes
            regulates thyroid hormone activation
  JOURNAL   J Clin Invest 112 (2), 189-196 (2003)
   PUBMED   12865408
  REMARK    GeneRIF: VDU1 has a role in amplifying the increase in type 2
            iodothyronine deiodinase activity that results from
            catecholamine-stimulated de novo synthesis
REFERENCE   8  (bases 1 to 4327)
  AUTHORS   Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C.
  TITLE     Human and mouse proteases: a comparative genomic approach
  JOURNAL   Nat Rev Genet 4 (7), 544-558 (2003)
   PUBMED   12838346
  REMARK    Review article
REFERENCE   9  (bases 1 to 4327)
  AUTHORS   Li,Z., Wang,D., Na,X., Schoen,S.R., Messing,E.M. and Wu,G.
  TITLE     Identification of a deubiquitinating enzyme subfamily as substrates
            of the von Hippel-Lindau tumor suppressor
  JOURNAL   Biochem Biophys Res Commun 294 (3), 700-709 (2002)
   PUBMED   12056827
REFERENCE   10 (bases 1 to 4327)
  AUTHORS   Li,Z., Na,X., Wang,D., Schoen,S.R., Messing,E.M. and Wu,G.
  TITLE     Ubiquitination of a novel deubiquitinating enzyme requires direct
            binding to von Hippel-Lindau tumor suppressor protein
  JOURNAL   J Biol Chem 277 (7), 4656-4662 (2002)
   PUBMED   11739384
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF383173.1, BC016663.1 and
            AI569971.1.
            
            On Jan 10, 2020 this sequence version replaced NM_201624.2.
            
            Summary: This gene encodes a deubiquinating enzyme important in a
            variety of processes, including Slit-dependent cell migration and
            beta-2 adrenergic receptor signaling. The protein is negatively
            regulated through ubiquitination by von Hippel-Lindau tumor protein
            (VHL). Alternative splicing results in multiple transcript variants
            and protein isoforms. [provided by RefSeq, Jun 2012].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 5' coding region and uses a downstream start codon
            compared to variant 1. The encoded isoform (2) has a shorter
            N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF383173.1, AB029020.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000370794.7/ ENSP00000359830.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-888               AF383173.1         1-888
            889-1823            BC016663.1         949-1883
            1824-4323           AF383173.1         1824-4323
            4324-4327           AI569971.1         19-22               c
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p31.1"
     gene            1..4327
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /note="ubiquitin specific peptidase 33"
                     /db_xref="GeneID:23032"
                     /db_xref="HGNC:HGNC:20059"
                     /db_xref="MIM:615146"
     exon            1..210
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            211..342
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     CDS             262..2997
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /EC_number="3.4.19.12"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ubiquitin carboxyl-terminal hydrolase 33; pVHL-interacting
                     deubiquitinating enzyme 1; ubiquitin thioesterase 33;
                     deubiquitinating enzyme 33; VHL-interacting
                     deubiquitinating enzyme 1; ubiquitin-specific-processing
                     protease 33; ubiquitin thiolesterase 33"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 33 isoform
                     2"
                     /protein_id="NP_963918.1"
                     /db_xref="CCDS:CCDS679.1"
                     /db_xref="GeneID:23032"
                     /db_xref="HGNC:HGNC:20059"
                     /db_xref="MIM:615146"
                     /translation="MSAFRNHCPHLDSVGEITKEDLIQKSLGTCQDCKVQGPNLWACL
                     ENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTQPSL
                     PHVRQPHQIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIG
                     NTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVV
                     PTTLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEVEEDPQTITTEETM
                     EEDKSQSDVDFQSCESCSNSDRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQK
                     EKMCNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDLSTP
                     QILPSNEGVNPRLSASPPKSGNLWPGLAPPHKKAQSASPKRKKQHKKYRSVISDIFDG
                     TIISSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTSIVKAGSCGEAYAP
                     QGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLR
                     NGVKFCKVQNFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIV
                     TYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYR
                     KSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGV
                     PPRKAGYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTELE
                     IFIRLNRAFQKEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGNVM
                     LRQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDILQAEEKIEVETRSL"
     exon            343..396
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            397..459
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            460..612
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            613..715
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            716..785
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            786..899
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            900..978
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1396
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1537
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1650
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1823
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1918
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..1952
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..1998
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            1999..2179
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            2180..2306
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            2307..2476
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2558
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..2667
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            2668..2770
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            2771..2839
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..4327
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4302..4307
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /note="hexamer: AATAAA"
     polyA_site      4327
                     /gene="USP33"
                     /gene_synonym="VDU1"
                     /note="major polyA site"
ORIGIN      
        1 ggagacaccg gaaggagccg cggctgctgc gggaagtggc cagttcagga ggcggacccc
       61 ccgagggcag cgctgcgggg ccgttttccg gccctcctga cgcgacactg cccctctccg
      121 agagctgaga aggaaaagag gagcttgcgg aggtgcggct gcaggccgtt gttggtcgag
      181 ctggcgggtc ccgcgggcca ggccgtggag gtgttacctc attttgaaag tcttgggaaa
      241 caggaaaaaa ttcctaacaa aatgtcagct tttcgaaatc attgtccaca tttggattca
      301 gttggtgaaa taacaaaaga agatttgata caaaaatccc ttggtacttg tcaggattgt
      361 aaagtccaag gaccaaatct ttgggcatgt ctggagaata gatgttcata tgttggctgt
      421 ggtgaatcac aagtagatca cagcaccata cattctcagg agacaaagca ttatctaact
      481 gtgaacctta ccactcttcg agtatggtgt tatgcttgca gcaaagaagt atttttggat
      541 aggaaattag gaactcagcc ttcattgcct catgtaagac aacctcacca aatacaagaa
      601 aacagtgtcc aggattttaa aatacccagt aatacaacat taaaaactcc tctggttgcc
      661 gtatttgatg atctggatat agaagcggat gaagaagatg aacttagggc cagaggtctt
      721 acaggtttga aaaatattgg aaatacttgt tacatgaatg cagctttgca ggctctttct
      781 aattgcccac ctttgacaca gttttttctt gattgtggag gactagctcg aacagataag
      841 aaacctgcca tttgtaaaag ttatctcaaa ctaatgacag agctgtggca taaaagcagg
      901 ccaggatctg ttgtgcctac tactctgttt caaggaatta aaactgtaaa tccaacattt
      961 cgggggtatt ctcagcagga tgctcaagaa ttccttcgat gtttaatgga tttgcttcat
     1021 gaagaattga aagagcaagt catggaagta gaagaagatc cgcaaaccat aaccactgag
     1081 gagacaatgg aagaagacaa gagccagtcg gatgtagatt ttcagtcttg tgaatcttgt
     1141 agcaacagtg atagagcaga aaatgaaaat ggctctagat gcttttctga agataataat
     1201 gaaacaacaa tgttaattca ggatgatgaa aacaattcag aaatgtcaaa ggattggcaa
     1261 aaagagaaga tgtgcaataa gattaataaa gtaaattctg aaggcgaatt tgataaagat
     1321 agagactcta tatctgaaac agtcgactta aacaaccagg aaactgtcaa agtgcaaata
     1381 cacagcagag cttcagaata tatcactgat gtccattcga atgacctgtc tacaccacag
     1441 atccttccat caaatgaagg tgttaatcca cgtttatcgg caagccctcc taaatcaggc
     1501 aatttgtggc caggattggc accaccacac aaaaaagctc agtctgcatc tccaaagaga
     1561 aaaaaacagc acaagaaata cagaagtgtt atttcagaca tatttgatgg aacaatcatt
     1621 agttcagtgc agtgtctgac ttgtgacagg gtgtctgtaa ccctcgagac ctttcaagat
     1681 ctgtccttgc caattcctgg caaggaagac cttgctaagc tgcattcatc aagtcatcca
     1741 acttctatag tcaaagcagg atcatgtggc gaagcatatg ctccacaagg gtggatagct
     1801 tttttcatgg aatatgtgaa gaggtttgtt gtctcatgtg tccctagctg gttttggggt
     1861 ccagtagtaa ccttgcaaga ttgtcttgct gccttctttg ccagagatga actaaaaggt
     1921 gacaatatgt acagttgtga aaaatgcaaa aagttgagaa atggagtgaa gttttgtaaa
     1981 gtacaaaact ttcctgagat tttgtgcatc caccttaaaa gattcagaca tgaactaatg
     2041 ttttccacca aaatcagtac ccatgtttca tttccgctag aaggcttgga tcttcagcca
     2101 tttcttgcta aggatagtcc agctcaaatt gtgacatatg atcttctgtc agtcatttgc
     2161 catcatggaa ctgcaagtag tggacactat atagcctact gccgaaacaa tctaaataat
     2221 ctctggtatg aatttgatga tcagagtgtc actgaagttt cagaatctac tgtacaaaat
     2281 gcagaagctt acgttctttt ctataggaag agcagcgaag aggcacaaaa agagaggaga
     2341 aggatatcaa atttattgaa cataatggaa ccaagcctcc ttcagtttta tatttctcga
     2401 cagtggctta ataaatttaa gacctttgcc gaacctggcc ctatttcaaa taatgacttt
     2461 ctttgtattc atggaggtgt tcctccaaga aaagctggtt atattgaaga cctggttttg
     2521 atgctgcctc agaacatttg ggataaccta tatagcaggt atggtggagg accagctgtc
     2581 aaccatctgt acatttgtca tacttgccaa attgaggcgg agaaaattga aaaaagaaga
     2641 aaaactgaat tggaaatttt tattcggctt aacagagcgt tccaaaaaga ggactctcca
     2701 gctacttttt attgcatcag tatgcagtgg tttagagaat gggaaagttt tgtgaagggt
     2761 aaagatggag atcctccagg tcctattgac aatactaaga ttgcagtcac taaatgtggt
     2821 aatgtgatgc ttaggcaagg agcagattct ggccagattt ctgaagaaac atggaatttt
     2881 ctgcagtcta tttatggtgg agggcctgaa gttatcctgc gacctccggt tgttcatgtt
     2941 gatccagata tacttcaagc agaagaaaaa attgaagtag aaactcggtc tttgtaattt
     3001 ttaggatgta gagagttcta atgaggaatc attttcatgt gccctgacat gtacacatgc
     3061 gaaaacattc ctaaaagcgt gtttatttgc tttatttttt ttcatcattt atcccattta
     3121 tttcttctta gtgggcatta tggaagaata tattaaaatg tgtaatatac cacaggttgg
     3181 tatatttagt tttaaatact taccataaag tctttcagtg taattttttt ttgagacaga
     3241 gtcttgcttt gtcacccagg ctggagtgct gtggtgttac ctcagctcac tgcagcctcc
     3301 acctcctggg ttcaagcgat tctcctgcct cagcctctcg agtagctggg attacaggca
     3361 cctgccacca tgcccggcta atttttgtat tttagtagag atggggtttc accatgttgg
     3421 ccaggctagt ctcaaactcc tgacctcagg tgatccaccc acctcggcct cccaaagtgc
     3481 tgggattaca ggtgtgagcc acagcgcctg gcccagtgta atatttttga aagaggaggg
     3541 acaattgtga aatcagtagg ttatctttaa tctttacact acatgcagat ccatagtatc
     3601 ctttgtagtg ttgtaaatac ttttgctttg aaaacttttt cattgtccta aatcaccctg
     3661 actctgacca gtctttcagt tctccaaaag cccaatttaa ttgtatagtt ttgtcatggc
     3721 ttcatataat aaagagccta ttttaagttg aaagtagtag tcagaaaatt gttaatttcc
     3781 taaagctcag gaaactaggg tgtcactttt tttgcactgc agcatataca ctaactagct
     3841 tattaaaatt tacaaaatgt ctttttgaat gtatcaagga tatatttagt ttgagtggaa
     3901 tttgtcagca gatatcagta acttattgcc gcttatattg tacaatgtta aacttcaatt
     3961 cctgtaacct ggttagtatt aatgtcagtg actaaaaaac ttagagttag ttttagggca
     4021 ctttttattt tgagagcatg aagtgtggaa tgtgtcacta cgattgttga taaagctgag
     4081 gccacttgca acttgatttt ttaaatgaaa tagataaagt ctttttgaat aatatagtat
     4141 gcactgctat ttgcttgatt atgtaatgtc aaaagtttaa ctatattcca agtacaaaaa
     4201 catactggat tacattgagg atgttgaata gcattcatga tggctttgtt ttggtttggg
     4261 gcagctgtca ccagctaaag caatgttgtt aaaattagct caataaaaat gtctttaaaa
     4321 tgtaaaa
//
