LOCUS       NM_020718              10881 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 31 (USP31), transcript
            variant 1, mRNA.
ACCESSION   NM_020718 XM_049683
VERSION     NM_020718.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Yu,Y., Chen,G., Jiang,C., Guo,T., Tang,H., Yuan,Z., Wang,Y.,
            Tan,X., Chen,J., Zhang,E. and Wang,X.
  TITLE     USP31 serves as a potential biomarker for predicting prognosis and
            immune responses for clear cell renal cell carcinoma via
            single-cell and bulk RNA-sequencing
  JOURNAL   J Gene Med 26 (1), e3594 (2024)
   PUBMED   37699648
  REMARK    GeneRIF: USP31 serves as a potential biomarker for predicting
            prognosis and immune responses for clear cell renal cell carcinoma
            via single-cell and bulk RNA-sequencing.
REFERENCE   2  (bases 1 to 10881)
  AUTHORS   Hou,Y., Fan,Y., Xia,X., Pan,J., Hou,J., Liu,X. and Chen,X.
  TITLE     USP31 acetylation at Lys1264 is essential for its activity and
            cervical cancer cell growth
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 53 (8), 1037-1043 (2021)
   PUBMED   34184746
  REMARK    GeneRIF: USP31 acetylation at Lys1264 is essential for its activity
            and cervical cancer cell growth.
REFERENCE   3  (bases 1 to 10881)
  AUTHORS   Quesada,V., Diaz-Perales,A., Gutierrez-Fernandez,A., Garabaya,C.,
            Cal,S. and Lopez-Otin,C.
  TITLE     Cloning and enzymatic analysis of 22 novel human ubiquitin-specific
            proteases
  JOURNAL   Biochem Biophys Res Commun 314 (1), 54-62 (2004)
   PUBMED   14715245
REFERENCE   4  (bases 1 to 10881)
  AUTHORS   Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C.
  TITLE     Human and mouse proteases: a comparative genomic approach
  JOURNAL   Nat Rev Genet 4 (7), 544-558 (2003)
   PUBMED   12838346
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC099482.3 and AC127459.3.
            
            On Sep 25, 2020 this sequence version replaced NM_020718.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ586135.2, AK126752.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000219689.12/ ENSP00000219689.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-815               AC099482.3         98137-98951
            816-953             AC127459.3         34980-35117
            954-1042            AC127459.3         36676-36764
            1043-1135           AC127459.3         36858-36950
            1136-1271           AC127459.3         37587-37722
            1272-1416           AC127459.3         40700-40844
            1417-1597           AC127459.3         52359-52539
            1598-1709           AC127459.3         55328-55439
            1710-1804           AC127459.3         55977-56071
            1805-1882           AC127459.3         57501-57578
            1883-2012           AC127459.3         58174-58303
            2013-2132           AC127459.3         60606-60725
            2133-2358           AC127459.3         62992-63217
            2359-2517           AC127459.3         69283-69441
            2518-2670           AC127459.3         70966-71118
            2671-10881          AC127459.3         73547-81757
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p12.2"
     gene            1..10881
                     /gene="USP31"
                     /note="ubiquitin specific peptidase 31"
                     /db_xref="GeneID:57478"
                     /db_xref="HGNC:HGNC:20060"
                     /db_xref="MIM:619536"
     exon            1..815
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..4241
                     /gene="USP31"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin specific proteinase 31; ubiquitin
                     carboxyl-terminal hydrolase 31; ubiquitin thioesterase 31;
                     deubiquitinating enzyme 31; ubiquitin-specific-processing
                     protease 31; ubiquitin thiolesterase 31"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 31 isoform
                     1"
                     /protein_id="NP_065769.3"
                     /db_xref="CCDS:CCDS10607.1"
                     /db_xref="GeneID:57478"
                     /db_xref="HGNC:HGNC:20060"
                     /db_xref="MIM:619536"
                     /translation="MSKVTAPGSGPPAAASGKEKRSFSKRLFRSGRAGGGGAGGPGAS
                     GPAAPSSPSSPSSARSVGSFMSRVLKTLSTLSHLSSEGAAPDRGGLRSCFPPGPAAAP
                     TPPPCPPPPASPAPPACAAEPVPGVAGLRNHGNTCFMNATLQCLSNTELFAEYLALGQ
                     YRAGRPEPSPDPEQPAGRGAQGQGEVTEQLAHLVRALWTLEYTPQHSRDFKTIVSKNA
                     LQYRGNSQHDAQEFLLWLLDRVHEDLNHSVKQSGQPPLKPPSETDMMPEGPSFPVCST
                     FVQELFQAQYRSSLTCPHCQKQSNTFDPFLCISLPIPLPHTRPLYVTVVYQGKCSHCM
                     RIGVAVPLSGTVARLREAVSMETKIPTDQIVLTEMYYDGFHRSFCDTDDLETVHESDC
                     IFAFETPEIFRPEGILSQRGIHLNNNLNHLKFGLDYHRLSSPTQTAAKQGKMDSPTSR
                     AGSDKIVLLVCNRACTGQQGKRFGLPFVLHLEKTIAWDLLQKEILEKMKYFLRPTVCI
                     QVCPFSLRVVSVVGITYLLPQEEQPLCHPIVERALKSCGPGGTAHVKLVVEWDKETRD
                     FLFVNTEDEYIPDAESVRLQRERHHQPQTCTLSQCFQLYTKEERLAPDDAWRCPHCKQ
                     LQQGSITLSLWTLPDVLIIHLKRFRQEGDRRMKLQNMVKFPLTGLDMTPHVVKRSQSS
                     WSLPSHWSPWRRPYGLGRDPEDYIYDLYAVCNHHGTMQGGHYTAYCKNSVDGLWYCFD
                     DSDVQQLSEDEVCTQTAYILFYQRRTAIPSWSANSSVAGSTSSSLCEHWVSRLPGSKP
                     ASVTSAASSRRTSLASLSESVEMTGERSEDDGGFSTRPFVRSVQRQSLSSRSSVTSPL
                     AVNENCMRPSWSLSAKLQMRSNSPSRFSGDSPIHSSASTLEKIGEAADDKVSISCFGS
                     LRNLSSSYQEPSDSHSRREHKAVGRAPLAVMEGVFKDESDTRRLNSSVVDTQSKHSAQ
                     GDRLPPLSGPFDNNNQIAYVDQSDSVDSSPVKEVKAPSHPGSLAKKPESTTKRSPSSK
                     GTSEPEKSLRKGRPALASQESSLSSTSPSSPLPVKVSLKPSRSRSKADSSSRGSGRHS
                     SPAPAQPKKESSPKSQDSVSSPSPQKQKSASALTYTASSTSAKKASGPATRSPFPPGK
                     SRTSDHSLSREGSRQSLGSDRASATSTSKPNSPRVSQARAGEGRGAGKHVRSSSMASL
                     RSPSTSIKSGLKRDSKSEDKGLSFFKSALRQKETRRSTDLGKTALLSKKAGGSSVKSV
                     CKNTGDDEAERGHQPPASQQPNANTTGKEQLVTKDPASAKHSLLSARKSKSSQLDSGV
                     PSSPGGRQSAEKSSKKLSSSMQTSARPSQKPQ"
     exon            816..953
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1042
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1135
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1271
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1416
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1597
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1709
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..1804
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1882
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..2012
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2013..2132
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2358
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2517
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2518..2670
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     exon            2671..10881
                     /gene="USP31"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4306
                     /gene="USP31"
                     /note="major polyA site"
     regulatory      8151..8156
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP31"
                     /note="hexamer: AATAAA"
     polyA_site      8170
                     /gene="USP31"
                     /note="major polyA site"
     regulatory      10852..10857
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP31"
                     /note="hexamer: AATAAA"
     regulatory      10861..10866
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP31"
                     /note="hexamer: ATTACA"
     polyA_site      10869
                     /gene="USP31"
     polyA_site      10881
                     /gene="USP31"
ORIGIN      
        1 gcgcgcgcgc ccgctggccc cgccggccgc cgctcgctcg ctggctcggc tgcgctttga
       61 ggtgtgtggg gcgtcgagcc cccgggcccg gtgaggcgcg gcgctgcggg atgcggcggc
      121 ggccgtggcg gggccgcggg ccgggcgggc gggcgcggtg atgagtgtct gcggccgccg
      181 ccatgtccaa ggtaacggcg cctgggtccg ggccgccggc ggcggcgagc gggaaggaga
      241 agcgctcctt cagcaagcgg ctgtttcgga gcggccgcgc tggcggcggc ggcgcggggg
      301 gccccggggc gtccgggccg gccgcgcctt cctcgccctc ctcgccctcc tctgcacgct
      361 cggtgggcag cttcatgagc cgcgttctca agacgctgtc cacgctctcg caccttagct
      421 ctgagggcgc cgccccagac cgcggcggcc tccgcagctg cttcccgccc gggccggccg
      481 ccgcgcccac gccgccgccg tgcccgccgc cgcccgcctc tcccgcgccg cccgcttgcg
      541 ccgccgagcc ggtgcccggc gtggcggggc tccgcaacca cggcaacacg tgcttcatga
      601 acgccacgct gcagtgcctc agcaacaccg agctcttcgc cgagtacctg gcgctgggcc
      661 agtaccgggc ggggcggccc gagccctcgc ctgacccgga gcagcctgcg ggccgcggcg
      721 cgcagggcca gggcgaggtc actgagcagc tggcgcacct ggtgcgggcc ctctggaccc
      781 tggagtacac cccgcagcac agccgcgact tcaagactat tgtgtcaaag aatgcactgc
      841 agtaccgggg aaattcccaa catgatgccc aggagtttct gctgtggctt ttggaccgag
      901 ttcatgaaga cctcaaccat tcagtgaagc agagtggcca gcctcctctg aagccaccat
      961 cagagactga tatgatgcct gagggaccat ctttccctgt ctgtagcact tttgtacaag
     1021 aactctttca agcgcaatac agatcttctt tgacgtgtcc tcattgtcag aaacagagca
     1081 acacttttga tcctttcctt tgcatttctt tgccaattcc tctgccccac acaaggcctc
     1141 tctatgtcac tgtagtgtat caaggcaaat gttctcactg catgaggatt ggtgtggccg
     1201 tacctctgtc tgggactgtc gccagacttc gggaagcagt gtctatggaa acaaagatcc
     1261 ccactgatca gattgtgtta acagaaatgt actatgatgg gttccatcgt tccttttgtg
     1321 atacagacga cctggaaaca gtccatgaaa gcgactgcat ttttgccttt gagactcccg
     1381 aaatatttag gcctgaagga attctcagtc aaagaggaat tcatttaaac aacaacctaa
     1441 accacttgaa atttggcttg gattatcata gactgtcttc tcctacacaa acagcagcaa
     1501 agcaggggaa aatggattct cccacatcaa gagcaggcag cgacaagatt gtcctgttgg
     1561 tgtgtaaccg agcctgcact gggcaacaag ggaaaagatt tggactgcct tttgtgctgc
     1621 acttagagaa gacaatagct tgggaccttc tgcagaagga aatcttggag aagatgaagt
     1681 atttcttgag gcccacggtt tgcattcagg tgtgtccatt cagcttgcgt gtggtcagtg
     1741 ttgttggaat aacatatttg ctgccccagg aggagcagcc cttgtgccac ccaatagtag
     1801 aaagggcatt aaaatcttgt ggaccaggtg gcactgctca tgtgaaatta gtagtcgagt
     1861 gggacaagga gacaagagat ttcttatttg taaatactga ggatgagtat attcctgatg
     1921 cagaaagtgt tcgtctgcaa agggagcgtc atcatcagcc tcaaacctgc actttatccc
     1981 agtgtttcca actgtacacc aaagaggagc ggcttgcccc cgatgatgcc tggcgttgcc
     2041 cacactgtaa gcagctgcag cagggaagca ttacgttaag cctctggact ctgcctgatg
     2101 tgcttattat acatctaaag agatttcggc aggaaggaga caggcgcatg aaacttcaga
     2161 acatggtcaa attccccttg actggcctgg acatgacacc tcacgtggtt aagaggagcc
     2221 agagcagctg gagtttgcca tcgcattggt ccccgtggag acggccctat ggactcggga
     2281 gggaccctga ggactacatc tatgacctgt atgctgtgtg caatcaccat ggcaccatgc
     2341 aaggggggca ctacacagcg tactgtaaga actctgtgga cggcctctgg tactgcttcg
     2401 atgacagcga tgtgcagcag ctgtcagaag atgaggtctg cacgcagaca gcatacatcc
     2461 tcttctacca gaggcggaca gccatcccgt catggtcagc caacagctcg gtggcaggct
     2521 ccacaagttc ttccctgtgt gaacactggg tgagccggct cccgggcagc aagccagcca
     2581 gcgtgacctc tgcagcttcc tccagacgca cctccctggc gtcgctctct gagtccgtgg
     2641 agatgactgg agaaaggagt gaagatgatg gaggcttttc aactcgacca tttgtgagaa
     2701 gtgtccagcg tcagagtttg tcatccagat cttctgtcac cagccccttg gccgtcaatg
     2761 aaaattgcat gagaccttca tggtccctgt ctgctaagct gcagatgcgc tccaattctc
     2821 catcccgatt ttcaggggat tcgccaattc acagctctgc ttccaccttg gagaagattg
     2881 gggaggcagc agatgacaag gtctccatct cttgctttgg tagcttgcgg aacctttcta
     2941 gcagttacca ggaaccaagc gacagtcata gtcgccgtga gcacaaggct gtgggccggg
     3001 cccctctggc tgtcatggaa ggcgtgttca aagacgaatc ggacacccgc agattgaact
     3061 ccagtgtcgt agatacacag agcaaacatt cagcacaagg ggaccgcctg cccccgctct
     3121 ctggtccatt tgataacaat aatcagatcg cttatgtgga tcagagcgac tccgtagaca
     3181 gctctccagt caaagaggtg aaagccccca gccacccagg ctcactcgca aagaaaccag
     3241 agagcacaac taagagatcc cccagttcca aaggcacttc tgagccagag aaaagcttgc
     3301 ggaaggggag accagccttg gcaagccagg agtcatccct ttcaagtaca tccccttctt
     3361 ctcctcttcc tgtaaaagtc tctctaaagc cctcccgctc ccgcagcaaa gcagattctt
     3421 cttccagggg cagtggacgg cattcatccc ctgcccctgc ccaacccaaa aaggagtcat
     3481 ccccgaaatc tcaggactcc gtgtcatctc cttcgccaca gaagcagaag tcagcctcgg
     3541 ccctcaccta cactgcttcc tccacatctg ccaaaaaggc ctcgggccct gccacaagga
     3601 gccctttccc acctgggaag agcaggactt cagaccacag cttgagtaga gagggctcca
     3661 gacaaagctt gggttctgac agagccagcg ccacctccac ctccaaaccc aattcccctc
     3721 gggtgagcca ggcccgagca ggggagggca ggggggccgg gaagcacgtg cggagctcct
     3781 ccatggccag cctgcgctcc cccagcacaa gcatcaagtc tggtttgaag agggacagca
     3841 agtctgagga caaggggctg tccttcttca aatcagcctt gagacagaag gaaacccggc
     3901 gctcgacgga tcttggcaag acagccttgc tctctaaaaa ggctggtggg agctctgtta
     3961 agtctgtctg taagaacacc ggggacgacg aggcagagag aggccaccag cctccagctt
     4021 cccagcagcc aaatgcaaat acaacgggaa aagagcagct tgtcaccaag gaccctgctt
     4081 ctgccaaaca ttccctgctg tccgctcgca aatccaagtc ttcccaacta gactctggag
     4141 ttccctcgtc tccgggtggc aggcagtctg cagagaaatc ctcaaaaaag ttatcttcta
     4201 gcatgcaaac ctctgcacgg ccttctcaaa aacctcagtg atatttctgc aatcaaagtg
     4261 ttttatctgt aaagatgttt atttatttag aacccctgcc ctcccaccaa agcctcctgt
     4321 gcttttgttt tgtacttttt tgagtcacgt gcccgactgt gtgtgtgtgt gtgagtgtat
     4381 gcgtgtgtgt gtctaattca attgcaaatt gttcaaagcg tcgccttatt ctgccattga
     4441 accaaataac agccataggc aaaacattga caccaagcta actggaataa ttgtagacga
     4501 tacccaggac ggtcccttta gcaattgtac atatttcttt ctagagaact ctaatgactt
     4561 tctttggata tgagggtttt gaaacctgtg aaccagaaaa gctaccatta gtgcgtagaa
     4621 ggaggaagga gctgactcgt gtttcttctg agcaggactt gtccttactg tggattgtgg
     4681 gtggcaccag gagctctaaa aactgtgact ctaacaacga aacaaatcga gggaaaaact
     4741 cttgctttct gcactttcgc cccatctacc agtctttgta aagggcaata ttttaacact
     4801 aatgtttctc aggtatatac tcagctagtc taggatggtt gcacaccagt aaatggatta
     4861 aagaggaagg tgccatgtgg gtgactgtgt catttctcct actgccacag atagacattt
     4921 tcgaggtaga aatgaagcct ttcaccacct tcctatctgt ggtgacatgt gcaccaaaac
     4981 ctgtcttgac tcttgggata ggtcctagga agtggcagat tggatagcca gaaagccagt
     5041 cactcgttta attttttttc cttctgaaat tactagctaa ggctcttgga attttgcact
     5101 gtagtagagc agttaacacc tttgacagat tcctggaaaa aacttctaga ttctaaactg
     5161 ggtgaaagac tggacatcat tttccttgtc aggtgttgca tttttccgtt agaggtggga
     5221 agctccacga tgccctgtgt ctgtgggctc atgttccctc atcttgtagc ttggagagga
     5281 aagaagttgc cttctgccaa aagccaatgg tggtatgttg aagcgttgtg tgatcaagtg
     5341 ttacatatgc acttcagatc ctgtctgtca gtctctgtta gggctgcctt tcagtaactc
     5401 atttattatt tcttctttgt tcttaagatt tcatctcatg cccagaactc acaagcaggt
     5461 tttggagtgt gttccatctg gccgtgtcag tgcaaattgc atttcttaca aaggagaacc
     5521 tcaccaaaaa aaacctcaca gataagcaaa gtatatagtt ttgcaagctt tttcaaggtc
     5581 tcctggcaat actttcctgc tcaattttta gcttttcttt tttttttaaa tgtcaagaga
     5641 gtgaaggtct tgattctctc tgaatagcat ttgtcacttt gccagtaaat acagcatgct
     5701 aagtttatgt gcttttaaat attagcgttt ttcgtcagac tcttcaagtt cttcttgaac
     5761 ccttggtgca cagatccata tataacctcc cttttcagta ttgctgtgtg tgaattaact
     5821 acaacacata tgcatgcata gctgtgaatt ctgcactact ttttttttct tttttccccc
     5881 caagaatatc tcttgggaaa agtttttagt acgttactta actttatttt gctgctaatt
     5941 tgcgcattag ccggtaactt gcaagtctgg agaccactta ctgttgaggg tagctggaat
     6001 tttagaccct tggcagtatt taagatttag acatttagcc ttgtgaaagt taccacagtg
     6061 ctccatgtga ttcattgact gtgagcctgt tgtccattgc actcagccct gtactcactt
     6121 cttcactcgc tgtcctggtt ttactgttca gattttggtg ggttctcaaa gcagtaccac
     6181 tctcatctgc tcagtacatc tgcggcaact ggctgccttc gatgctgtca ttgcgttcag
     6241 cccagcacat cacagcccca tcagtgtgta gccaccgctc tcgtttttct gtatcgcttt
     6301 tatctcctga aaaagatgca ccaaataaga gatcatctat atcaggaatt gaatgtctaa
     6361 ctcaaacctc tcatttttgt ttaataggta gatctcattt gaagtctcct atctgaatct
     6421 ctgaaacaat tatagtttga ttagacaaag gtttttaata acacttcttt tttgctgtag
     6481 tcaagcatta atagaaacta gtttaatttt cacagtggta aatattttta ttcttgagtt
     6541 atctaggaat ggattttcca cgatgtctag tgacttaaaa aaaaaaagga aaaacgtttc
     6601 tacaaatccc tttggttttc catgggatct ggaataaaat gtcagtgtct ataaactgta
     6661 tgtcatggag tttagtcctt gtttcaggtt taatagaaaa aatcaggaag tatcatggca
     6721 ttgtctagac atcatgaagc tattttattt ccatagttgg taaacagtga aaatttcatt
     6781 atctccaact actaggtaca actttaggcc acaggaaagg ttatttggcc cgaaagtttg
     6841 gaggtgctcc ttgaggtggg aagtatcttg tgggaaagta gttacttgat tcaatttagt
     6901 tcattggtag ggttggggac ttggtgttgg tttctttctt tcttttcttt tttttttttt
     6961 ttttaatttt cccagcaata tttagcacaa tgagcgtgta tgtgctttgg ggttaaaaat
     7021 tatctgctag ggatgatagg cctgggtttc tcgttgacat ctagatgggc ctgattagag
     7081 cagtcatctc ctggtaggca caaaataatc accaacagta atgttgtatc tgtaatgttt
     7141 gtttgctttt taaagtttta ttcttgattt cttgtaaaca tccattgcac tgaggccgta
     7201 tcattcctta agacagccct acatcttgcc ttggtaatcc cttagataat gtggaaactc
     7261 aaaccgttag gctccagtca tcttagacca tttcccatcc gttttgtatt ttctcgtcct
     7321 gaaagcagct gaactgaaag taagtaataa tatgctggac cagtgggaac ggatgtggga
     7381 gtatttgtgg tgtgctaaaa tactgtaatt atctgtgagg ctgcctgaca cgcttttgca
     7441 atcttgtttt catcattcat tctgcaaacg tttatggagg gccttctttg agcacagagg
     7501 caaaactgaa agcttcaggg atttgctgta gctgaggagc cctgggagca cagcccatca
     7561 agcaagggat gtgatggtgt cctaaatgga gtgacagttc tcctgggaaa agagatcacg
     7621 tggattccgg tcaaatcggc taaggcttgt ggttctcttg agcaagtctg ttccttctgg
     7681 aaaccaaaag tgcccttcat ctttagagac attttactct tccatccacc ttttcagact
     7741 gagctgtctc tactatttag gggttgaaaa tccattacac agtcacttta cgttaacatt
     7801 gggtcatctt atgtttgtat gagacggatg tgggatttga ggggaacatt gtcattccct
     7861 taaaataact atcatgaaaa aaatagcaac agtatgtaaa gggaccaaga gtggcagcct
     7921 ttagggaaaa tggagcagag aagagctgtg aacaaagctc atttatttga aataagcgtg
     7981 ctttatttca catagagggg catcagacgt tacatttcat tcggacaaat tactgaacag
     8041 ccgaagtgat tgtttccaat gtaggttatt ataattattg gatacaaaga tttaactagt
     8101 gtttctgggt tggatttttg aaatagtatg caagtcataa gcacagtttc aataaaacac
     8161 gtattctgga ggtgcgtgga actttgtatt atttgtagtt gagaaaggca aactttgctg
     8221 acaaaccagt acctatcaca aacgaacaca aaaagtagct gttactgtgc aggctgtctt
     8281 taactttcat ttcataaagg gtacattatt gttaaactct actacatggt ttggggagaa
     8341 atccagtggt tttatttaaa tgaacactct cacactgcct tttgatcaac tttcaaagta
     8401 ttttgcagat gcattttgag catgaccatt tttttcccca agtaaaaaca attgtgcgtg
     8461 gcctccatat aactcagaaa gcatgctctt actacctatg caagcaaaca ataaaacata
     8521 tagacatgaa atgttaagtc ttaaaaaaaa aaggcaacaa ttatggagct ctatttttaa
     8581 atttgtgatt ttcaaaatat ggcttactca tcattgtgta gaagagcatg aatgcgattt
     8641 cttgttttgc tctgcatata tttctctgct gtttttctga tcattggcga gattgtaaag
     8701 tattttgcaa gcagaagtgg aattggatat gttgaacaca catcattggt gtacatactg
     8761 tatttagcct gaagactttc ctaaagtgtt ttgttctaaa aataacctgc cattttctat
     8821 agattttaat tttttatatc ttcaagggtt ctattttggt cacaaggaaa gaatgtggct
     8881 tctaatagtg ctaagaatta aaagatgctt ttatagagac ttagagctat tttgaaaagc
     8941 tttggggctt cccagccact gatcacaatt agactaagcc catggaattt ctgggagtgt
     9001 tgtgcagtgg attcggttct agatccatct tacatttgtt tagtgtttcc caatttttgg
     9061 gagaaggggt gcacagcaaa tttgagagac ttttttgaat ctcacacatg tattttcctc
     9121 aagattcttt taagcctctt ctgaatgact tgtctgagat agtgaagacc cacattaact
     9181 agaatcagct ggggaacttt aaaaaataca gtgcctgggt cccagtccct agcttttgat
     9241 ctaattcatg taggggtggg atccaggaat taccatgtat tttaaaagct tctgggtgtt
     9301 tctaatttgc agacagattg agaaccacta tgatacgtca tccctttact gagaattatg
     9361 gcgagaatca ctgccatttg aattactcag gagtgactga ttcctcagtt gtgtaggaga
     9421 agcagaaaaa ataagaataa aaagaattga gagatactga tatcaaactc ccatttgaaa
     9481 tacaagggcc cttctgattc aaggcccagt tggggagctt ggaccatgtt gtgaatggcc
     9541 tagcactcgc cagtctggtg gaaattggtg gtcttagttg ctggagcagg ctgagtgtcc
     9601 ccgcggggtt tgcatctgtc cccagaacct tttccccagg cccgtggggg aatagggaag
     9661 tcactctgag ctagaaattg tcgaagaaga aaagaaacaa ccttcctagc cttggaaagc
     9721 ctgtccctgc ttttgataga attattgcct ttttgtagga attacagtag caaaccccag
     9781 gctgccgctg aatttcctac ttttgctcat tctctaggat agttcttatc cagaatgtgg
     9841 tttcaggaat tctttttttt agtttgaaaa tgaatgtgaa acattttctt tggtgtcttt
     9901 tttttaaaaa aaaaaaaccc ttattgctat tttttcgtgt ttttgttttg ttttgttttt
     9961 tgtttgtttt accattaagt aatacacatc ccagaatttg gcactctgtg ttaactgtgg
    10021 aggcaaataa aatttagttt taatctgtgg tgagctgaat gcactacaac accttcgaag
    10081 taatctgatg gtttcattct gtcgagcctc atgctgccta caaaacacca caatttacct
    10141 ggtgttttca acattttaag gaatgaagat acaagtagac atgcaagttg gaaatgtttc
    10201 tttagaaagg ccatccagaa taatgatatc tgttgcagtc gtccaaatgc aatatgaaat
    10261 ttcctaggtg ctattataaa cggtgtggtg tcataccaaa tgcaaaagca gttacagtat
    10321 ttccgaagtt ggagttctct ttgtaggcct ttcaaatacg tgtttgcatt ccatagaggt
    10381 ctgctcacca actgtgcctc tcaccactgg ttaaaattga atttaagcct cattgacata
    10441 agtcgtttct tgattgaact cttcagtttt tacactggaa tttctgtagt tgtcttgaaa
    10501 gctgcatgtc acatcactgt acacaaagtg ctttggtatt actcatgcac cgtggatagt
    10561 gatttctgtg tcgttttcat tttcagttgt acagtaatat ggaaggcagg atgtctttcc
    10621 tagaggctgg aagaaaagtg tatcgtacat tttaaattta ttttagaaca agtgcatgag
    10681 atgtgtcact gatttctgtt atttatttgt atgttttatt attcaaagtg tatgcacttt
    10741 taagaagcag aatttatatt tttacgtttg aaacgtttta tttctgaaac agcgttgatt
    10801 atatagtata cagttggaat taagagaaaa gtggaaaatg taacaaaata caataaattt
    10861 attacaagat gtcctcttca a
//
