LOCUS       NM_006537               5517 bp    mRNA    linear   PRI 23-JUL-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 3 (USP3), transcript
            variant 1, mRNA.
ACCESSION   NM_006537
VERSION     NM_006537.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Tao,R., Liu,Z., Zhang,Z. and Zhang,Z.
  TITLE     USP3 promotes cisplatin resistance in non-small cell lung cancer
            cells by suppressing ACOT7-regulated ferroptosis
  JOURNAL   Anticancer Drugs 35 (6), 483-491 (2024)
   PUBMED   38502867
  REMARK    GeneRIF: USP3 promotes cisplatin resistance in non-small cell lung
            cancer cells by suppressing ACOT7-regulated ferroptosis.
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Zhang,H., Liu,W., Wu,Y. and Chen,C.
  TITLE     USP3: Key deubiquitylation enzyme in human diseases
  JOURNAL   Cancer Sci 115 (7), 2094-2106 (2024)
   PUBMED   38651282
  REMARK    GeneRIF: USP3: Key deubiquitylation enzyme in human diseases.
            Review article
REFERENCE   3  (bases 1 to 5517)
  AUTHORS   Li,A., Wang,S., Nie,J., Xiao,S., Xie,X., Zhang,Y., Tong,W., Yao,G.,
            Liu,N., Dan,F., Shu,Z., Liu,J., Liu,Z. and Yang,F.
  TITLE     USP3 promotes osteosarcoma progression via deubiquitinating EPHA2
            and activating the PI3K/AKT signaling pathway
  JOURNAL   Cell Death Dis 15 (3), 235 (2024)
   PUBMED   38531846
  REMARK    GeneRIF: USP3 promotes osteosarcoma progression via
            deubiquitinating EPHA2 and activating the PI3K/AKT signaling
            pathway.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5517)
  AUTHORS   Xu,J., Gui,G.S.W., Yang,C., Zhu,S., Chen,Z., Ma,S.L.B., Yang,C.,
            Bu,C.R.L., Zhu,Y. and Xu,W.
  TITLE     USP3 inhibition is Active Against Chemo-resistant Hepatocellular
            Carcinoma Anchorage-independent Growth via Suppressing
            Wnt/beta-catenin
  JOURNAL   Curr Mol Med 24 (5), 667-675 (2024)
   PUBMED   37921189
  REMARK    GeneRIF: USP3 inhibition is Active Against Chemo-resistant
            Hepatocellular Carcinoma Anchorage-independent Growth via
            Suppressing Wnt/beta-catenin.
REFERENCE   5  (bases 1 to 5517)
  AUTHORS   Karapurkar,J.K., Kim,M.S., Colaco,J.C., Suresh,B., Sarodaya,N.,
            Kim,D.H., Park,C.H., Hong,S.H., Kim,K.S. and Ramakrishna,S.
  TITLE     CRISPR/Cas9-based genome-wide screening of the deubiquitinase
            subfamily identifies USP3 as a protein stabilizer of REST blocking
            neuronal differentiation and promotes neuroblastoma tumorigenesis
  JOURNAL   J Exp Clin Cancer Res 42 (1), 121 (2023)
   PUBMED   37170124
  REMARK    GeneRIF: CRISPR/Cas9-based genome-wide screening of the
            deubiquitinase subfamily identifies USP3 as a protein stabilizer of
            REST blocking neuronal differentiation and promotes neuroblastoma
            tumorigenesis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5517)
  AUTHORS   Dato,S., Soerensen,M., Montesanto,A., Lagani,V., Passarino,G.,
            Christensen,K. and Christiansen,L.
  TITLE     UCP3 polymorphisms, hand grip performance and survival at old age:
            association analysis in two Danish middle aged and elderly cohorts
  JOURNAL   Mech Ageing Dev 133 (8), 530-537 (2012)
   PUBMED   22743239
  REMARK    GeneRIF: the role of UCP3 gene in functional status and survival at
            old age.
REFERENCE   7  (bases 1 to 5517)
  AUTHORS   Smith,N.L., Felix,J.F., Morrison,A.C., Demissie,S., Glazer,N.L.,
            Loehr,L.R., Cupples,L.A., Dehghan,A., Lumley,T., Rosamond,W.D.,
            Lieb,W., Rivadeneira,F., Bis,J.C., Folsom,A.R., Benjamin,E.,
            Aulchenko,Y.S., Haritunians,T., Couper,D., Murabito,J., Wang,Y.A.,
            Stricker,B.H., Gottdiener,J.S., Chang,P.P., Wang,T.J., Rice,K.M.,
            Hofman,A., Heckbert,S.R., Fox,E.R., O'Donnell,C.J.,
            Uitterlinden,A.G., Rotter,J.I., Willerson,J.T., Levy,D., van
            Duijn,C.M., Psaty,B.M., Witteman,J.C., Boerwinkle,E. and Vasan,R.S.
  TITLE     Association of genome-wide variation with the risk of incident
            heart failure in adults of European and African ancestry: a
            prospective meta-analysis from the cohorts for heart and aging
            research in genomic epidemiology (CHARGE) consortium
  JOURNAL   Circ Cardiovasc Genet 3 (3), 256-266 (2010)
   PUBMED   20445134
  REMARK    GeneRIF: Observational study, meta-analysis, and genome-wide
            association study of gene-disease association. (HuGE Navigator)
REFERENCE   8  (bases 1 to 5517)
  AUTHORS   Nicassio,F., Corrado,N., Vissers,J.H., Areces,L.B., Bergink,S.,
            Marteijn,J.A., Geverts,B., Houtsmuller,A.B., Vermeulen,W., Di
            Fiore,P.P. and Citterio,E.
  TITLE     Human USP3 is a chromatin modifier required for S phase progression
            and genome stability
  JOURNAL   Curr Biol 17 (22), 1972-1977 (2007)
   PUBMED   17980597
  REMARK    GeneRIF: Ubiquitin-specific protease 3 (USP3) is a deubiquitinating
            enzyme for monoubiquitinated histones H2A and H2B; USP3 dynamically
            associates with chromatin and deubiquitinates H2A/H2B in vivo.
REFERENCE   9  (bases 1 to 5517)
  AUTHORS   Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C.
  TITLE     Human and mouse proteases: a comparative genomic approach
  JOURNAL   Nat Rev Genet 4 (7), 544-558 (2003)
   PUBMED   12838346
  REMARK    Review article
REFERENCE   10 (bases 1 to 5517)
  AUTHORS   Sloper-Mould,K.E., Eyre,H.J., Wang,X.W., Sutherland,G.R. and
            Baker,R.T.
  TITLE     Characterization and chromosomal localization of USP3, a novel
            human ubiquitin-specific protease
  JOURNAL   J Biol Chem 274 (38), 26878-26884 (1999)
   PUBMED   10480896
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK314223.1 and AC118274.9.
            
            On Nov 23, 2018 this sequence version replaced NM_006537.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.181811.1,
                                           SRR1660809.210464.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380324.8/ ENSP00000369681.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1710              AK314223.1         2-1711
            1711-5517           AC118274.9         103340-107146
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.31"
     gene            1..5517
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /note="ubiquitin specific peptidase 3"
                     /db_xref="GeneID:9960"
                     /db_xref="HGNC:HGNC:12626"
                     /db_xref="MIM:604728"
     exon            1..238
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     CDS             148..1710
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /EC_number="3.4.19.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-specific-processing protease 3; deubiquitinating
                     enzyme 3; ubiquitin carboxyl-terminal hydrolase 3;
                     ubiquitin thioesterase 3; ubiquitin thiolesterase 3"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 3 isoform
                     1"
                     /protein_id="NP_006528.2"
                     /db_xref="CCDS:CCDS32265.1"
                     /db_xref="GeneID:9960"
                     /db_xref="HGNC:HGNC:12626"
                     /db_xref="MIM:604728"
                     /translation="MECPHLSSSVCIAPDSAKFPNGSPSSWCCSVCRSNKSPWVCLTC
                     SSVHCGRYVNGHAKKHYEDAQVPLTNHKKSEKQDKVQHTVCMDCSSYSTYCYRCDDFV
                     VNDTKLGLVQKVREHLQNLENSAFTADRHKKRKLLENSTLNSKLLKVNGSTTAICATG
                     LRNLGNTCFMNAILQSLSNIEQFCCYFKELPAVELRNGKTAGRRTYHTRSQGDNNVSL
                     VEEFRKTLCALWQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLEL
                     QGGFNGVSRSAILQENSTLSASNKCCINGASTVVTAIFGGILQNEVNCLICGTESRKF
                     DPFLDLSLDIPSQFRSKRSKNQENGPVCSLRDCLRSFTDLEELDETELYMCHKCKKKQ
                     KSTKKFWIQKLPKVLCLHLKRFHWTAYLRNKVDTYVEFPLRGLDMKCYLLEPENSGPE
                     SCLYDLAAVVVHHGSGVGSGHYTAYATHEGRWFHFNDSTVTLTDEETVVKAKAYILFY
                     VEHQAKAGSDKL"
     exon            239..299
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            300..431
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            432..515
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            516..597
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            598..680
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            681..794
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            795..908
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1055
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1162
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1243
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1362
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1476
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1544
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..5517
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2307..2312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /note="hexamer: AATAAA"
     polyA_site      2348
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /note="major polyA site"
     regulatory      5497..5502
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /note="hexamer: ATTAAA"
     polyA_site      5517
                     /gene="USP3"
                     /gene_synonym="SIH003; UBP"
                     /note="major polyA site"
ORIGIN      
        1 ctttctttga cgcaagggct cgagacgcag ccgccgtcgg ccgagcgccc ggctagaagc
       61 gacaccagac ggagcctccg gagttcctcc gcccccacct cgccgggtcc tggagccgca
      121 gtcctcccag ctgccctcct cgtggccatg gagtgtccac acctgagctc cagcgtctgc
      181 attgctccgg actcagccaa gttccccaac ggctccccgt cgtcctggtg ctgcagcgtg
      241 tgccggtcca acaaaagccc ttgggtctgt ttgacttgtt caagtgtcca ctgtggaagg
      301 tatgtgaatg gccatgcaaa aaaacattat gaagatgcac aagtaccttt aaccaaccat
      361 aagaaatcag aaaagcaaga taaagttcag cacacagtat gtatggattg cagtagctac
      421 agtacatact gttatcgctg tgatgatttt gtggttaatg acaccaagct gggactggta
      481 cagaaagtca gagaacactt acagaacttg gaaaactcag ctttcacagc tgacaggcat
      541 aagaaaagaa aacttttgga aaactcaaca ctaaacagca agttattaaa agtaaatgga
      601 agcaccactg ccatttgtgc cacaggcctt cggaatttgg ggaacacatg tttcatgaat
      661 gccatccttc agtcactcag taacattgag cagttttgct gttatttcaa agaactgccc
      721 gccgtggagt taaggaatgg gaaaacagca ggaaggcgga cataccacac caggagccaa
      781 ggggataaca atgtgtcttt ggtagaagag tttagaaaga cactctgtgc tttatggcaa
      841 ggcagccaga ctgcatttag cccagagtcc ttattttatg ttgtttggaa gattatgcca
      901 aactttaggg gctatcaaca gcaggacgcc catgaattca tgcgctacct tttggaccac
      961 ctacacttgg aacttcaggg cggtttcaac ggtgtttccc gctcagcaat tctgcaggag
     1021 aattctactc tgtctgcaag taacaagtgt tgcataaatg gagcatctac tgttgtcacg
     1081 gctatattcg gaggcattct ccaaaatgag gttaactgcc tcatatgtgg gacagaatct
     1141 agaaagtttg atccattcct agacctttca ttagatattc caagtcagtt cagaagtaag
     1201 cgctctaaga atcaagaaaa tggaccagtt tgttcgttac gagattgtct tcgcagtttt
     1261 accgacttag aagaacttga tgagacagag ttatatatgt gccataaatg caaaaagaaa
     1321 caaaagtcca caaaaaagtt ttggattcaa aaactaccca aggtgctatg cttacatttg
     1381 aaaagatttc attggacagc atatttaaga aataaagttg atacatacgt agaatttcca
     1441 ctgagaggcc tagacatgaa atgctactta ctagagcctg agaacagtgg cccggagagc
     1501 tgcctgtatg acctcgccgc tgtggtggtg caccatggtt ccggggttgg ttctggacat
     1561 tacacagcat acgcaactca cgaaggccgc tggttccact tcaatgacag tactgtaaca
     1621 ctgactgacg aggagactgt ggtgaaggcg aaggcctaca tccttttcta cgtggaacac
     1681 caggccaaag ctggatcgga taaactttaa tacctcctcc aaatcatcat tcaccaacca
     1741 taccagagaa acatttccag ttttccacaa atacttgata caagatttaa tttcattatg
     1801 cacttttcaa tttcctattt tggatttagt tttgtcaatg gtagtgactt actgaacatg
     1861 ggcaccaact aattttgttg ttgttctacc agaaaacctc agcagatgtt ttgatttgct
     1921 gctttagttg taataattca atttttatag gtagttgtaa gaacttagtc ttatttgact
     1981 tttttatttt atgttaatgt tttcagttct cactttgagg cacatttaca tcaatgcttt
     2041 tgttcctctc acatgctgaa agcaagatgt gttccttatt gtgaagagcg acacaactgc
     2101 ctgctgcctt tccacagcta taatggacat caggttgact ctaaatcaag gatcatgtgt
     2161 gcacaatact tgtggcccac aaaatttcac aatgactgct gaggaatcat tctttttgcc
     2221 tgtaaaatat aacaaagggc atcattaagt agaccaggta attactgctt gtctctcaag
     2281 gctgctgtct ttatcagcac taactaaata aatttgttgg ttcagttgta cttgtcctgc
     2341 aaatacaaga attactctct ttgttggttt ttttggtttt ggggcatact tgtttgcggg
     2401 gaggtaagat gggagtaaag accaaataca tgtaatgttt aaaaaaaatg ctgtgactca
     2461 ctgacatggt ataggtgtta ccagtgaggt aaagccatgg cgtctgcctc cttttgaatc
     2521 agtatctcat tcccccttag ttgtaccact ggaagtcacg acatgtccat tatagactta
     2581 cttactgctt gtgtttggca tctgagtctg aggtgtgacc ctctgggatt tacttggatc
     2641 atcacccgca cttcagttta tcactggggg gtgtgcttcc acaggaggaa atttagaatc
     2701 ttcgctgctc tgggttttaa cacctctctc tggcctatcc tgattgatag gacaagttga
     2761 aaatactgtt ggagtaagta agtggagttt tcgttttgtt ttttgggggt ttttggagac
     2821 aagagtctct ctctgttgcc caggctggag tgcagtggca cgatctcagc tcattgcaac
     2881 ctcggcctcc cgagtagcaa ttctcctgcc tcaacctcct gagtagctgg gattacaggt
     2941 gcccaccact acacctggct aatttttttt ttgtattttt agtaaagacg ggggtttacc
     3001 atgttggcta gggtggtctc aacctccctc ctgacctcag gtgatccgcc tgcctcagcc
     3061 tcccaaagtg ctgagattac aggcgtgagc catcacgccc agacagtttt gtttttaaaa
     3121 aacagattta actcaaagat tcaaatttgg aaccagaatc ccatttaaag aaagcagttg
     3181 tggtttctac aagtgttcta agacagtcct gtggtggttt tttttttttc ttttttttgg
     3241 ttgggggcgg tgggggttgg tagacatagc ctaccaattc tccacaaggg acaaaatgta
     3301 tcttgtgtta agccaacgag actttctggc aatttttttt tttttttttt aatggagtct
     3361 tactctgtcc ctcaggctgg agtgcagtgt ggcatgatca cagctcactg tagcctcaac
     3421 ctcccaggct caagcgatcc tcccacttca gcctcgaagt agctggcacc accacaccca
     3481 gctaattttt aatattttta ctagagatgg ggtttcacca cgttgcccag gctggtcttg
     3541 cactcctggg ctcaagtgat ccacccacct tggcttccca aggtgctagc attacaggca
     3601 tgagccacca cgcccagcct gttctattaa aaacatcttg accttttttt ttttttaagt
     3661 ttatgatata aaaacagtct ggtggctttc cctcaggttg gatatctgaa gttgtgggag
     3721 cagatgtagc tccatttcca ttttaatatt aactggataa atcccacatc ttcccagaac
     3781 acatctgcta aacactttaa catgacaagg agcaaattac aattatgtgt aaacttatat
     3841 catccagtac aatggcccct agccacatgt gactgaaatt taaattagaa actcagtgcc
     3901 ttcagtcaca ctagccacat ttcaagtgct cagcacccac atacggcgta ggacaatgct
     3961 ggtctaaatt ttcactccaa agaataaaac tttgccatca tgtttaggtc ttacaacttt
     4021 gagtcctaaa agataatagg gagatgtgtg cttaaattaa tttacaatgc taaattatat
     4081 taggttaaac tgtatgaaac cctgctattt gatgagttct gatctacaaa aatgacagct
     4141 ccacctgatc tgatgtatca cacattaaca tcacatctaa ttcaacctga aatgtgagtc
     4201 tcctttgatc ttgcaaagta tctcctatga atccgggctc ttttgcttag aaggtagaca
     4261 gctgtaacct ctaacattga tacttcgatc ttcagtctct ctgaatgggc agctgttaaa
     4321 cagtaaaggc ctgggcagag ttgatggtga aaaaaagctg tatttttata ccaaatggaa
     4381 cttcagcaac acgttggact actttgtcgg gcagagtggt cttcagacag gtgatgctgt
     4441 tggcttaaga gatgaacacg tcttctctct gaatttgttt ttattggttg gttttacttg
     4501 aacagaaacg tttgaatggt gtgaagattc tttagaaaag tgattggatc catctcaatt
     4561 gtgcacttga acagcagtga tgtaaatgtg cttatttgtg ttttcatttt taactgtgca
     4621 gccttaccac acctattttg aaagctgatc ttttgtcctc ttcagggctt ttcccttgtg
     4681 ccatgttgtc tccagcaata tatgtggctt ctggctcaga gcttctgctg aggcagggcc
     4741 tcgcgctgag tcacatggct ccttggaaca agaaaacttt ctcagaagtc cccacacggg
     4801 tgggtgacag aaacagtttg tggcaacaca aatctctctc catggtggag aaacctttgc
     4861 ttatccactg tattctactc agtgtatagt gatgacagat gtactagaat taaaccagtc
     4921 aagtgtactg gcccggggtg agtgttaaat gtgtatctcg cctgtggaac ctactcagca
     4981 gcttggtttc tggggttggg ggcatattta tttcttaaag ccaaacctct ccttcattgg
     5041 tttagtgtac ataagtctat ttagatacac ttatcccact ttaatctcca ccagccccta
     5101 aactctctcc tgtaccctct gttgctgaag tggcctcttc acctgggccc tggaaaagcc
     5161 gatggaaaaa gtctgttcct gcagccaagg cctgggtccc aggtgactag gagtagccat
     5221 aggaaggtga ggaaggagaa gtgggcatgt ggtgtcatct gtagtccaag gtctctgcgt
     5281 ctcgtcagtg ggtgcagtgc cttccccctc aggtgtgagc gtgatggact ctagactgcc
     5341 ttccatgagt gttggtcatg ctatgatgtc atcatttgcc attaaaaccc agtttgtatg
     5401 atgccaagag gattaattgt gcaagtgact gattcattta aattgtaatc acatcccttt
     5461 tctgcttcac caacctgaac tgtttttaaa aatatcatta aaagtctgtt ctctttc
//
