LOCUS       NM_001346252            4856 bp    mRNA    linear   PRI 13-DEC-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript
            variant 3, mRNA.
ACCESSION   NM_001346252 XM_005271631
VERSION     NM_001346252.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Jin,C., Einig,E., Xu,W., Kollampally,R.B., Schlosser,A., Flentje,M.
            and Popov,N.
  TITLE     The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions
            to limit DNA damage
  JOURNAL   Nucleic Acids Res 52 (6), 3011-3030 (2024)
   PUBMED   38227944
  REMARK    GeneRIF: The dimeric deubiquitinase USP28 integrates 53BP1 and MYC
            functions to limit DNA damage.
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Meitinger,F., Belal,H., Davis,R.L., Martinez,M.B., Shiau,A.K.,
            Oegema,K. and Desai,A.
  TITLE     Control of cell proliferation by memories of mitosis
  JOURNAL   Science 383 (6690), 1441-1448 (2024)
   PUBMED   38547292
  REMARK    GeneRIF: Control of cell proliferation by memories of mitosis.
REFERENCE   3  (bases 1 to 4856)
  AUTHORS   Karapurkar,J.K., Colaco,J.C., Suresh,B., Tyagi,A., Woo,S.H.,
            Jo,W.J., Ko,N., Singh,V., Hong,S.H., Oh,S.J., Kim,K.S. and
            Ramakrishna,S.
  TITLE     USP28 promotes tumorigenesis and cisplatin resistance by
            deubiquitinating MAST1 protein in cancer cells
  JOURNAL   Cell Mol Life Sci 81 (1), 145 (2024)
   PUBMED   38498222
  REMARK    GeneRIF: USP28 promotes tumorigenesis and cisplatin resistance by
            deubiquitinating MAST1 protein in cancer cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4856)
  AUTHORS   Zhou,W., Chen,J. and Wang,J.
  TITLE     Comprehensive prognostic and immunological analysis of Ubiquitin
            Specific Peptidase 28 in pan-cancers and identification of its role
            in hepatocellular carcinoma cell lines
  JOURNAL   Aging (Albany NY) 15 (13), 6545-6576 (2023)
   PUBMED   37450415
  REMARK    GeneRIF: Comprehensive prognostic and immunological analysis of
            Ubiquitin Specific Peptidase 28 in pan-cancers and identification
            of its role in hepatocellular carcinoma cell lines.
REFERENCE   5  (bases 1 to 4856)
  AUTHORS   Li,J., Peng,J., Wu,L., Shen,X., Zhen,X., Zhang,Y., Ma,H., Xu,Y.,
            Xiong,Q., Zhu,Q. and Zhang,P.
  TITLE     The deubiquitinase USP28 maintains the expression of the
            transcription factor MYCN and is essential in neuroblastoma cells
  JOURNAL   J Biol Chem 299 (7), 104856 (2023)
   PUBMED   37230388
  REMARK    GeneRIF: The deubiquitinase USP28 maintains the expression of the
            transcription factor MYCN and is essential in neuroblastoma cells.
REFERENCE   6  (bases 1 to 4856)
  AUTHORS   Popov,N., Herold,S., Llamazares,M., Schulein,C. and Eilers,M.
  TITLE     Fbw7 and Usp28 regulate myc protein stability in response to DNA
            damage
  JOURNAL   Cell Cycle 6 (19), 2327-2331 (2007)
   PUBMED   17873522
  REMARK    GeneRIF: Usp28 dissociates from Fbw7alpha in response to UV
            irradiation, providing a mechanism how Fbw7-mediated degradation of
            Myc is enhanced upon DNA damage.
REFERENCE   7  (bases 1 to 4856)
  AUTHORS   Popov,N., Wanzel,M., Madiredjo,M., Zhang,D., Beijersbergen,R.,
            Bernards,R., Moll,R., Elledge,S.J. and Eilers,M.
  TITLE     The ubiquitin-specific protease USP28 is required for MYC stability
  JOURNAL   Nat Cell Biol 9 (7), 765-774 (2007)
   PUBMED   17558397
  REMARK    GeneRIF: High expression levels of USP28 are found in colon and
            breast carcinomas, and stabilization of MYC by USP28 is essential
            for tumour-cell proliferation.
REFERENCE   8  (bases 1 to 4856)
  AUTHORS   Zhang,D., Zaugg,K., Mak,T.W. and Elledge,S.J.
  TITLE     A role for the deubiquitinating enzyme USP28 in control of the
            DNA-damage response
  JOURNAL   Cell 126 (3), 529-542 (2006)
   PUBMED   16901786
  REMARK    GeneRIF: Using a human cell line that faithfully recapitulated the
            Chk2-p53-PUMA pathway, we show that USP28 is required to stabilize
            Chk2 and 53BP1 in response to DNA damage.
REFERENCE   9  (bases 1 to 4856)
  AUTHORS   Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C.
  TITLE     Human and mouse proteases: a comparative genomic approach
  JOURNAL   Nat Rev Genet 4 (7), 544-558 (2003)
   PUBMED   12838346
  REMARK    Review article
REFERENCE   10 (bases 1 to 4856)
  AUTHORS   Valero,R., Bayes,M., Francisca Sanchez-Font,M., Gonzalez-Angulo,O.,
            Gonzalez-Duarte,R. and Marfany,G.
  TITLE     Characterization of alternatively spliced products and
            tissue-specific isoforms of USP28 and USP25
  JOURNAL   Genome Biol 2 (10), RESEARCH0043 (2001)
   PUBMED   11597335
  REMARK    GeneRIF: molecular cloning of USP28 & characterization of
            alternatively spliced products and tissue-specific isoforms
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP001874.4, AP003170.4 and
            KF459544.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 3, 2021 this sequence version replaced NM_001346252.3.
            
            Summary: The protein encoded by this gene is a deubiquitinase
            involved in the DNA damage pathway and DNA damage-induced
            apoptosis. Overexpression of this gene is seen in several cancers.
            [provided by RefSeq, Oct 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.988446.1,
                                           SRR18074967.2842996.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000696973.1/ ENSP00000513009.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               AP001874.4         7291-7418           c
            129-206             AP003170.4         110545-110622       c
            207-339             AP003170.4         108788-108920       c
            340-445             AP003170.4         97950-98055         c
            446-605             AP003170.4         96885-97044         c
            606-692             AP003170.4         90536-90622         c
            693-830             AP003170.4         89707-89844         c
            831-904             AP003170.4         88207-88280         c
            905-981             AP003170.4         87154-87230         c
            982-1130            AP003170.4         85484-85632         c
            1131-1258           AP003170.4         83520-83647         c
            1259-1354           AP003170.4         79892-79987         c
            1355-1534           AP003170.4         73945-74124         c
            1535-1743           AP003170.4         71461-71669         c
            1744-1814           AP003170.4         70172-70242         c
            1815-2043           AP003170.4         68563-68791         c
            2044-2235           AP003170.4         65350-65541         c
            2236-2375           AP003170.4         64585-64724         c
            2376-2561           AP003170.4         64238-64423         c
            2562-2657           AP003170.4         62776-62871         c
            2658-2836           AP003170.4         61155-61333         c
            2837-2915           AP003170.4         60960-61038         c
            2916-2995           AP003170.4         60085-60164         c
            2996-3119           AP003170.4         59445-59568         c
            3120-3315           AP003170.4         57770-57965         c
            3316-3599           KF459544.1         112-395
            3600-4856           AP003170.4         54162-55418         c
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.2"
     gene            1..4856
                     /gene="USP28"
                     /note="ubiquitin specific peptidase 28"
                     /db_xref="GeneID:57646"
                     /db_xref="HGNC:HGNC:12625"
                     /db_xref="MIM:610748"
     exon            1..128
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..3491
                     /gene="USP28"
                     /EC_number="3.4.19.12"
                     /note="isoform c is encoded by transcript variant 3;
                     ubiquitin thioesterase 28; deubiquitinating enzyme 28;
                     ubiquitin-specific-processing protease 28; ubiquitin
                     thiolesterase 28"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 28 isoform
                     c"
                     /protein_id="NP_001333181.1"
                     /db_xref="CCDS:CCDS91596.1"
                     /db_xref="GeneID:57646"
                     /db_xref="HGNC:HGNC:12625"
                     /db_xref="MIM:610748"
                     /translation="MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEAL
                     KASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDL
                     QAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGW
                     PVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQEL
                     QYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVN
                     VNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAM
                     VEGDVELLPSDHSVKYGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQII
                     YMDRYMYRSKELIRNKRECIRKLKEEIKILQQKLERYVKYGSGPARFPLPDMLKYVIE
                     FASTKPASESCPPESDTHMTLPLSSVHCSVSDQTSKESTSTESSSQDVESTFSSPEDS
                     LPKSKPLTSSRSSMEMPSQPAPRTVTDEEINFVKTCLQRWRSEIEQDIQDLKTCIAST
                     TQTIEQMYCDPLLRQVPYRLHAVLVHEGQANAGHYWAYIYNQPRQSWLKYNDISVTES
                     SWEEVERDSYGGLRNVSAYCLMYINDKLPYFNAEAAPTESDQMSEVEALSVELKHYIQ
                     EDNWRFEQEVEEWEEEQSCKIPQMESSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAV
                     IVKEQTAQAIANTARAYEKSGVEAALSELKEAEPKKPMPQETNLAEQSEQPPKANDAE
                     STAQPNSEVSEVEIPSVGRILVRSDADGYDEEVMLSPAMQGVILAIAKARQTFDRDGS
                     EAGLIKAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFAD
                     KNLSYDERSISIMKVAQAKLKEIGPDDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQ
                     KGKYQEALSYLVYAYQSNAALLMKGPRRGVKESVIALYRRKCLLELNAKAASLFETND
                     DHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVMRNHWCSYLGQDIAENLQLCL
                     GEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCSRFAAVMESIQGVSTVTVK"
     exon            129..206
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            207..339
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            340..445
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            446..605
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            606..692
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            693..830
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            831..904
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            905..981
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1130
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1258
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1354
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1355..1534
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1743
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1814
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            1815..2043
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2235
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..2375
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2561
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2562..2657
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2658..2836
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2837..2915
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2916..2995
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            2996..3119
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            3120..3315
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     exon            3316..4856
                     /gene="USP28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4838..4843
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP28"
                     /note="hexamer: AATAAA"
     polyA_site      4856
                     /gene="USP28"
                     /note="major polyA site"
ORIGIN      
        1 ctagtcctga gaggctgggc cggcggcggc tgcggcggga gaccggtgac ccgcggctgg
       61 gcgcctcggc catgactgcg gagctgcagc aggacgacgc ggccggcgcg gcagacggcc
      121 acggctcgag ctgccaaatg ctgttaaatc aactgagaga aatcacaggc attcaggacc
      181 cttcctttct ccatgaagct ctgaaggcca gtaatggtga cattactcag gcagtcagcc
      241 ttctcactga tgagagagtt aaggagccca gtcaagacac tgttgctaca gaaccatctg
      301 aagtagaggg gagtgctgcc aacaaggaag tattagcaaa agttatagac cttactcatg
      361 ataacaaaga tgatcttcag gctgccattg ctttgagtct actggagtct cccaaaattc
      421 aagctgatgg aagagatctt aacaggatgc atgaagcaac ctctgcagaa actaaacgct
      481 caaagagaaa acgctgtgaa gtctggggag aaaaccccaa tcccaatgac tggaggagag
      541 ttgatggttg gccagttggg ctgaaaaatg ttggcaatac atgttggttt agtgctgtta
      601 ttcagtctct ctttcaattg cctgaatttc gaagacttgt tctcagttat agtctgccac
      661 aaaatgtact tgaaaattgt cgaagtcata cagaaaagag aaatatcatg tttatgcaag
      721 agcttcagta tttgtttgct ctaatgatgg gatcaaatag aaaatttgta gacccgtctg
      781 cagccctgga tctattaaag ggagcattcc gatcatctga ggaacagcag caagatgtga
      841 gtgaattcac acacaagctc ctggattggc tagaggacgc attccagcta gctgttaatg
      901 ttaacagtcc caggaacaaa tctgaaaatc caatggtgca gctgttctat ggtactttcc
      961 tgactgaagg ggttcgtgaa ggaaaaccct tttgtaacaa tgagaccttc ggccagtatc
     1021 ctcttcaggt aaacggttat cgcaacttag acgagtgttt ggaaggggcc atggtggagg
     1081 gtgatgttga gcttcttccc tccgatcact cggtgaagta tggacaagag cgttggttta
     1141 caaagctacc tccagtgttg acctttgaac tctcaagatt tgagtttaat cagtcccttg
     1201 ggcagccaga gaaaattcac aataagctgg aatttcctca gattatttat atggacaggt
     1261 acatgtacag gagcaaggag cttattcgaa ataagagaga gtgtattcga aagttgaagg
     1321 aggaaataaa aattctgcag caaaaattgg aaaggtatgt gaaatatggc tcaggcccag
     1381 ctcggttccc gctcccggac atgctgaaat atgttattga atttgctagt acaaaacctg
     1441 cctcagaaag ctgtccacct gaaagtgaca cacatatgac attaccactt tcttcagtgc
     1501 actgctcggt ttctgaccag acatccaagg aaagtacaag tacagaaagc tcttctcagg
     1561 atgttgaaag taccttttct tctcctgaag attctttacc caagtctaaa ccactgacat
     1621 cttctcggtc ttccatggaa atgccttcac agccagctcc acgaacagtc acagatgagg
     1681 agataaattt tgttaagacc tgtcttcaga gatggaggag tgagattgaa caagatatac
     1741 aagatttaaa gacttgtatt gcaagtacta ctcagactat tgaacagatg tactgcgatc
     1801 ctctccttcg tcaggtgcct tatcgcttgc atgcagttct tgttcatgaa ggacaagcaa
     1861 atgctggaca ctattgggcc tatatctata atcaaccccg acagagctgg ctcaagtaca
     1921 atgacatctc tgttactgaa tcttcctggg aagaagttga aagagattcc tatggaggcc
     1981 tgagaaatgt tagtgcttac tgtctgatgt acattaatga caaactaccc tacttcaatg
     2041 cagaggcagc cccaactgaa tcagatcaaa tgtcagaagt ggaagcccta tctgtggaac
     2101 tcaagcatta cattcaggag gataactggc ggtttgagca ggaagtagag gagtgggaag
     2161 aagagcagtc ttgcaaaatc cctcaaatgg agtcctccac caactcctca tcacaggact
     2221 actctacatc acaagagcct tcagtagcct cttctcatgg ggttcgctgc ttgtcgtctg
     2281 agcatgctgt gattgtaaag gagcaaactg cccaggctat tgcaaacaca gcccgtgcct
     2341 atgagaagag cggtgtagaa gcggcactga gtgagcttaa agaagctgaa cccaagaagc
     2401 ccatgcccca ggaaacaaac cttgcagagc agtcagaaca gcccccaaag gctaatgatg
     2461 cagagtctac tgcccagcct aattctgagg tctctgaagt cgagattccc agtgtgggaa
     2521 ggattctggt tagatctgat gcagatggat atgatgagga ggtgatgctg agccctgcca
     2581 tgcaaggggt catcctggcc atagctaaag cccgtcagac ctttgaccga gatgggtctg
     2641 aagcagggct gattaaggca ttccatgaag aatactccag gctctatcag cttgccaaag
     2701 agacccccac ctctcacagt gatcctcgac ttcagcatgt ccttgtctac tttttccaaa
     2761 atgaagcacc caaaagggta gtagaacgaa cccttctgga acagtttgca gataaaaatc
     2821 ttagctatga tgaaagatca atcagcatta tgaaggtggc tcaagcgaaa ctgaaggaaa
     2881 ttggtccaga tgacatgaat atggaagagt acaagaagtg gcatgaagat tatagtttgt
     2941 tccgaaaagt gtctgtgtat ctcctaacag gcctagaact ctatcaaaaa ggaaagtacc
     3001 aagaggcact ttcctacctg gtatatgcct accagagcaa tgctgccctg ctgatgaagg
     3061 ggccccgccg gggggtcaaa gaatccgtga ttgctttata ccgaagaaaa tgccttctgg
     3121 agctgaatgc caaagcagct tctctttttg aaacaaatga tgatcactcc gtaactgagg
     3181 gcattaatgt gatgaatgaa ctgatcatcc cctgcattca ccttatcatt aataatgaca
     3241 tttccaagga tgatctggat gccattgagg tcatgagaaa ccattggtgc tcttaccttg
     3301 ggcaagatat tgcagaaaat ctgcagctgt gcctagggga gtttctaccc agacttctag
     3361 atccttctgc agaaatcatc gtcttgaaag agcctccaac tattcgaccc aattctccct
     3421 atgacctatg tagccgattt gcagctgtca tggagtcaat tcagggagtt tcaactgtga
     3481 cagtgaaata agctcccaca tgttcaaggc ccattctggt tcctggctgc ctgcctcttg
     3541 cacagaagtt cgttgtcata gtgctcacct tgggaaaagg attaggtggg cacataagat
     3601 tccgatcaga ccccaaccat gctgcatgtg taaagaagga ttgaaaataa aattgcactt
     3661 tttaggtaca aaatcataaa agctgtttca ctagaaaagg cagaaagcag tgtattaagg
     3721 tgttgaatta cgccagaaga cctgaaatgc cttgtaccta caacaatgct taggcttttc
     3781 taagcctctt gccactttta aaattatcct tcaggcataa atatttttga cagcagaata
     3841 gaagaatgat tcatgagaac ctgaaccaga tgaacagcta ctagttattt tatcaaatac
     3901 agatgacatt taaaaattct taactacaag agattagaaa tataaacctt gcctggctct
     3961 tgccaggaga taacaaaatg ggttgctgat gaactgcacc cttttacatg tgggtagaat
     4021 ataagctcac atggcagtga gatgttgaaa agtcaaaaga gacctgtctc tctcctttct
     4081 tttctatctt taaaccagaa aacctcatac tcagtcctca gtgaaagaaa gtaaagtatt
     4141 aaggacttta gacagaagag cattgtgtaa cttgactgaa gatcatccat taatagttat
     4201 taggcattta ggtaaaattt tctaatacct aaaaattgtc aaaaacagtc aatagggcta
     4261 ctgctggccc aaagaccatt taggtccacc tcctcttttt tgctcttttt ttttttctgt
     4321 gacagtttca ctgtgtcgcc caggctggcg ttcagtggtg caatctcagc tcactgcaaa
     4381 ctctgtctcc tgggctcaag tgattctcgt gcctcagcct cccgaatagc tggaattacg
     4441 ggcatgcacc accacacctg gctaattttt gtatttttaa tagagatggg gtttcaccat
     4501 attggccagg ctgatctcta actcctggcc tcaagtgatc tatctgcctc cctcagcctc
     4561 ccaaagtctg ggattgcaga caagtcatcg tacccggcct tcttttttgc ccttaaaagt
     4621 aagggatgtg ggtttgtaca aaaaaaaaaa aaaaaaaaaa aaaaccagca tacatatgca
     4681 aaactatata tatatgtata tgtagagaaa aatacttccc attgatcatt tttaaaaggc
     4741 ttctgattgg atattgtgtt ttaaccaaat tttaaagatt aatggaatca tgaaagggaa
     4801 aaaaattgat acaactatgc agattttata aatgtgcaat aaaagtattt gtttta
//
