LOCUS       NM_001014443            2315 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 21 (USP21), transcript
            variant 3, mRNA.
ACCESSION   NM_001014443
VERSION     NM_001014443.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Sun,X., Yu,J., Cui,X., Tang,Y. and Yu,Y.
  TITLE     Inhibition of USP21 leads to ovarian carcinoma cell death by
            suppressing MAPK signaling
  JOURNAL   Biotechnol Appl Biochem 71 (1), 232-239 (2024)
   PUBMED   37964466
  REMARK    GeneRIF: Inhibition of USP21 leads to ovarian carcinoma cell death
            by suppressing MAPK signaling.
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Liang,W., Liu,D. and Wu,J.
  TITLE     c-JUN-induced upregulation of LINC00174 contributes to colorectal
            cancer proliferation and invasion through accelerating USP21
            expression
  JOURNAL   Cell Biol Int 47 (11), 1782-1798 (2023)
   PUBMED   37434557
  REMARK    GeneRIF: c-JUN-induced upregulation of LINC00174 contributes to
            colorectal cancer proliferation and invasion through accelerating
            USP21 expression.
REFERENCE   3  (bases 1 to 2315)
  AUTHORS   Wang,Q.D., Liu,L.L., Li,D., Gao,L. and Zhang,M.J.
  TITLE     Salt-like transcription factor 4 promotes laryngeal cancer
            progression through transcriptional activation of
            ubiquitin-specific protease 21 to stabilize Yin Yang 1
  JOURNAL   Pathol Int 73 (3), 109-119 (2023)
   PUBMED   36285444
  REMARK    GeneRIF: Salt-like transcription factor 4 promotes laryngeal cancer
            progression through transcriptional activation of
            ubiquitin-specific protease 21 to stabilize Yin Yang 1.
REFERENCE   4  (bases 1 to 2315)
  AUTHORS   Wu,Y., Guo,Y. and Wang,Q.
  TITLE     USP21 accelerates the proliferation and glycolysis of esophageal
            cancer cells by regulating the STAT3/FOXO1 pathway
  JOURNAL   Tissue Cell 79, 101916 (2022)
   PUBMED   36095935
  REMARK    GeneRIF: USP21 accelerates the proliferation and glycolysis of
            esophageal cancer cells by regulating the STAT3/FOXO1 pathway.
REFERENCE   5  (bases 1 to 2315)
  AUTHORS   Zhang,Q., Chen,Z., Tang,Q., Wang,Z., Lu,J., You,Y. and Wang,H.
  TITLE     USP21 promotes self-renewal and tumorigenicity of mesenchymal
            glioblastoma stem cells by deubiquitinating and stabilizing FOXD1
  JOURNAL   Cell Death Dis 13 (8), 712 (2022)
   PUBMED   35974001
  REMARK    GeneRIF: USP21 promotes self-renewal and tumorigenicity of
            mesenchymal glioblastoma stem cells by deubiquitinating and
            stabilizing FOXD1.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2315)
  AUTHORS   Ye,Y., Akutsu,M., Reyes-Turcu,F., Enchev,R.I., Wilkinson,K.D. and
            Komander,D.
  TITLE     Polyubiquitin binding and cross-reactivity in the USP domain
            deubiquitinase USP21
  JOURNAL   EMBO Rep 12 (4), 350-357 (2011)
   PUBMED   21399617
  REMARK    GeneRIF: Study report that USP21 cleaves Ub polymers, and with
            reduced activity also targets ISG15, but is inactive against NEDD8.
REFERENCE   7  (bases 1 to 2315)
  AUTHORS   Xu,G., Tan,X., Wang,H., Sun,W., Shi,Y., Burlingame,S., Gu,X.,
            Cao,G., Zhang,T., Qin,J. and Yang,J.
  TITLE     Ubiquitin-specific peptidase 21 inhibits tumor necrosis factor
            alpha-induced nuclear factor kappaB activation via binding to and
            deubiquitinating receptor-interacting protein 1
  JOURNAL   J Biol Chem 285 (2), 969-978 (2010)
   PUBMED   19910467
  REMARK    GeneRIF: USP21 plays an important role in the down-regulation of
            TNFalpha-induced NF-kappaB activation through deubiquitinating
            RIP1.
REFERENCE   8  (bases 1 to 2315)
  AUTHORS   Sowa,M.E., Bennett,E.J., Gygi,S.P. and Harper,J.W.
  TITLE     Defining the human deubiquitinating enzyme interaction landscape
  JOURNAL   Cell 138 (2), 389-403 (2009)
   PUBMED   19615732
REFERENCE   9  (bases 1 to 2315)
  AUTHORS   Gong,L., Kamitani,T., Millas,S. and Yeh,E.T.
  TITLE     Identification of a novel isopeptidase with dual specificity for
            ubiquitin- and NEDD8-conjugated proteins
  JOURNAL   J Biol Chem 275 (19), 14212-14216 (2000)
   PUBMED   10799498
REFERENCE   10 (bases 1 to 2315)
  AUTHORS   Smith,T.S. and Southan,C.
  TITLE     Sequencing, tissue distribution and chromosomal assignment of a
            novel ubiquitin-specific protease USP23
  JOURNAL   Biochim Biophys Acta 1490 (1-2), 184-188 (2000)
   PUBMED   10786635
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI755746.1, BC090946.1,
            AF177758.1 and AI038040.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001014443.2.
            
            Summary: This gene encodes a member of the C19 peptidase family,
            also known as family 2 of ubiquitin carboxy-terminal hydrolases.
            The encoded protein cleaves ubiquitin from ubiquitinated proteins
            for recycling in intracellular protein degradation. The encoded
            protein is also able to release NEDD8, a ubiquitin-like protein,
            from NEDD8-conjugated proteins. This gene has been referred to as
            USP16 and USP23 but is now known as USP21. Alternatively spliced
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            coding sequence compared to variant 4. The resulting isoform (c) is
            shorter at the N-terminus compared to isoform a. Variants 1 and 3
            both encode the same isoform (c).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.822388.1,
                                           SRR1163657.189286.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368002.8/ ENSP00000356981.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                BI755746.1         51-66
            17-465              BC090946.1         1-449
            466-2122            AF177758.1         212-1868
            2123-2315           AI038040.1         8-200               c
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.3"
     gene            1..2315
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /note="ubiquitin specific peptidase 21"
                     /db_xref="GeneID:27005"
                     /db_xref="HGNC:HGNC:12620"
                     /db_xref="MIM:604729"
     exon            1..179
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            180..320
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            321..941
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     CDS             342..2039
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /EC_number="3.4.19.12"
                     /note="isoform c is encoded by transcript variant 3;
                     ubiquitin specific protease 21; ubiquitin thiolesterase
                     21; ubiquitin-specific processing protease 21;
                     deubiquitinating enzyme 21; ubiquitin carboxyl-terminal
                     hydrolase 21; ubiquitin-specific protease 16;
                     NEDD8-specific protease; ubiquitin thioesterase 21"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 21 isoform
                     c"
                     /protein_id="NP_001014443.1"
                     /db_xref="CCDS:CCDS30920.1"
                     /db_xref="GeneID:27005"
                     /db_xref="HGNC:HGNC:12620"
                     /db_xref="MIM:604729"
                     /translation="MPQASEHRLGRTREPPVNIQPRVGSKLPFAPRARSKERRNPASG
                     PNPMLRPLPPRPGLPDERLKKLELGRGRTSGPRPRGPLRADHGVPLPGSPPPTVALPL
                     PSRTNLARSKSVSSGDLRPMGIALGGHRGTGELGAALSRLALRPEPPTLRRSTSLRRL
                     GGFPGPPTLFSIRTEPPASHGSFHMISARSSEPFYSDDKMAHHTLLLGSGHVGLRNLG
                     NTCFLNAVLQCLSSTRPLRDFCLRRDFRQEVPGGGRAQELTEAFADVIGALWHPDSCE
                     AVNPTRFRAVFQKYVPSFSGYSQQDAQEFLKLLMERLHLEINRRGRRAPPILANGPVP
                     SPPRRGGALLEEPELSDDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRST
                     TFEVFCDLSLPIPKKGFAGGKVSLRDCFNLFTKEEELESENAPVCDRCRQKTRSTKKL
                     TVQRFPRILVLHLNRFSASRGSIKKSSVGVDFPLQRLSLGDFASDKAGSPVYQLYALC
                     NHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASSEGYVLFYQLMQEPPRCL"
     exon            942..1001
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1122
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1234
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1390
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            1391..1455
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1559
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1646
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1725
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1833
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1948
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2315
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2295..2300
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /note="hexamer: AATAAA"
     polyA_site      2315
                     /gene="USP21"
                     /gene_synonym="USP16; USP23"
                     /note="major polyA site"
ORIGIN      
        1 ggtggccggg cgggaccaac aaagatggcg gcggcccctg cggcgggagc gatctgggca
       61 acggctgcgg ctaaagctgc agccgggccc acgggggggc tgcacggggg tagtaggggg
      121 tggccctgaa ctggggcctg gccctggctg gcctctcccg ccgcctcact gggggacagg
      181 tactggggat acgatggctg attcgataga tctggttcct gccctcagtg cgtatactgc
      241 tccccacttt cgttgatgtg gtagtggtga tgactgagcc aaccacctaa tgtggaaatg
      301 agaggacagc aagattgtgg gtccagcctg tggtgtccac aatgccccag gcctctgagc
      361 accgcctggg ccgtacccga gagccacctg ttaatatcca gccccgagtg ggatccaagc
      421 taccatttgc ccccagggcc cgcagcaagg agcgcagaaa cccagcctct gggccaaacc
      481 ccatgttacg acctctgcct ccccggccag gtctgcctga tgaacggctc aagaaactgg
      541 agctgggacg gggacggacc tcaggccctc gtcccagagg cccccttcga gcagatcatg
      601 gggttcccct gcctggctca ccacccccaa cagtggcttt gcctctccca tctcggacca
      661 acttagcccg ttccaagtct gtgagcagtg gggacttgcg tccaatgggg attgccttgg
      721 gagggcaccg tggcaccgga gagcttgggg ctgcactgag ccgcttggcc ctccggcctg
      781 agccacccac tttgagacgt agcacttctc tccgccgcct agggggcttt cctggacccc
      841 ctaccctgtt cagcatacgg acagagcccc ctgcttccca tggctccttc cacatgatat
      901 ccgcccggtc ctctgagcct ttctactctg atgacaagat ggctcatcac acactccttc
      961 tgggctctgg tcatgttggc cttcgaaacc tgggaaacac gtgcttcctg aatgctgtgc
     1021 tgcagtgtct gagcagcact cgacctcttc gggacttctg tctgagaagg gacttccggc
     1081 aagaggtgcc tggaggaggc cgagcccaag agctcactga agcctttgca gatgtgattg
     1141 gtgccctctg gcaccctgac tcctgcgaag ctgtgaatcc tactcgattc cgagctgtct
     1201 tccagaaata tgttccctcc ttctctggat acagccagca ggatgcccaa gagttcctga
     1261 agctcctcat ggagcggcta caccttgaaa tcaaccgccg aggccgccgg gctccaccga
     1321 tacttgccaa tggtccagtt ccctctccac cccgccgagg aggggctctg ctagaagaac
     1381 ctgagttaag tgatgatgac cgagccaacc taatgtggaa acgttacctg gagcgagagg
     1441 acagcaagat tgtggacctg tttgtgggcc agttgaaaag ttgtctcaag tgccaggcct
     1501 gtgggtatcg ctccacgacc ttcgaggttt tttgtgacct gtccctgccc atccccaaga
     1561 aaggatttgc tgggggcaag gtgtctctgc gggattgttt caaccttttc actaaggaag
     1621 aagagctaga gtcggagaat gccccagtgt gtgaccgatg tcggcagaaa actcgaagta
     1681 ccaaaaagtt gacagtacaa agattccctc gaatcctcgt gctccatctg aatcgatttt
     1741 ctgcctcccg aggctccatc aaaaaaagtt cagtaggtgt agactttcca ctgcagcgac
     1801 tgagcctagg ggactttgcc agtgacaaag ccggaagtcc tgtataccag ctgtatgccc
     1861 tttgcaacca ctcaggcagc gtccactatg gccactacac agccctgtgc cggtgccaga
     1921 ctggttggca tgtctacaat gactctcgtg tctcccctgt cagtgaaaac caggtggcat
     1981 ccagcgaggg ctacgtgctg ttctaccaac tgatgcagga gccaccccgg tgcctgtgac
     2041 acctctaagc tctggcacct gtgaagccct ttaaacaccc ttaagcccca ggctccccgt
     2101 ttacctcaga gacgtctatt tttgtgtctt tttaatcggg gaggggggag ggggtggttg
     2161 tagctccatt atttttttta ttaaaaaata cccttccacc tggaggctcc cttgtctccc
     2221 agccccatgt acaaagctca ccaagcccct gcccatgtac agcccccaga ccctctgcaa
     2281 tatcactttt tgtgaataaa tttattaaga aaaaa
//
