LOCUS       NM_005151               4972 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens ubiquitin specific peptidase 14 (USP14), transcript
            variant 1, mRNA.
ACCESSION   NM_005151
VERSION     NM_005151.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Li,M., Zhao,Y., Zhang,J., Jiang,W., Peng,S., Hu,J. and Shen,Y.
  TITLE     Deubiquitinase USP14 is upregulated in Crohn's disease and inhibits
            the NOD2 pathway mediated inflammatory response in vitro
  JOURNAL   Eur J Histochem 68 (3), 4101 (2024)
   PUBMED   39252535
  REMARK    GeneRIF: Deubiquitinase USP14 is upregulated in Crohn's disease and
            inhibits the NOD2 pathway mediated inflammatory response in vitro.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Xu,M.H., Zheng,Y.M., Liang,B.G., Xu,W.X., Cao,J., Wang,P.,
            Dong,Z.Y., Zhou,C.H., Sun,H.C., Ren,N., Ke,A.W. and Shen,Y.H.
  TITLE     Deubiquitination of CIB1 by USP14 promotes lenvatinib resistance
            via the PAK1-ERK1/2 axis in hepatocellular carcinoma
  JOURNAL   Int J Biol Sci 20 (9), 3269-3284 (2024)
   PUBMED   38993552
  REMARK    GeneRIF: Deubiquitination of CIB1 by USP14 promotes lenvatinib
            resistance via the PAK1-ERK1/2 axis in hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4972)
  AUTHORS   Dash,R., Tran,N.N., Lee,S.B. and Lee,B.H.
  TITLE     Structural Dynamics Analysis of USP14 Activation by AKT-Mediated
            Phosphorylation
  JOURNAL   Cells 13 (11), 955 (2024)
   PUBMED   38891087
  REMARK    GeneRIF: Structural Dynamics Analysis of USP14 Activation by
            AKT-Mediated Phosphorylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4972)
  AUTHORS   Ding,L., Lu,L., Zheng,S., Zhang,Z., Huang,X., Ma,R., Zhang,M.,
            Xu,Z., Chen,M., Guo,Z., Zhu,S., Gong,J., Mao,H., Zhang,W. and Xu,P.
  TITLE     Usp14 deficiency removes alpha-synuclein by regulating S100A8/A9 in
            Parkinson's disease
  JOURNAL   Cell Mol Life Sci 81 (1), 232 (2024)
   PUBMED   38780644
  REMARK    GeneRIF: Usp14 deficiency removes alpha-synuclein by regulating
            S100A8/A9 in Parkinson's disease.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4972)
  AUTHORS   Wei,D., Tian,X., Ren,Z., Liu,Z. and Sun,C.
  TITLE     Mechanistic insights into the role of USP14 in adipose tissue
            macrophage recruitment and insulin resistance in obesity
  JOURNAL   Int J Biol Macromol 267 (Pt 2), 131645 (2024)
   PUBMED   38631582
  REMARK    GeneRIF: Mechanistic insights into the role of USP14 in adipose
            tissue macrophage recruitment and insulin resistance in obesity.
REFERENCE   6  (bases 1 to 4972)
  AUTHORS   Hu,M., Li,P., Song,L., Jeffrey,P.D., Chenova,T.A., Wilkinson,K.D.,
            Cohen,R.E. and Shi,Y.
  TITLE     Structure and mechanisms of the proteasome-associated
            deubiquitinating enzyme USP14
  JOURNAL   EMBO J 24 (21), 3747-3756 (2005)
   PUBMED   16211010
  REMARK    GeneRIF: the catalytic cleft leading to the active site of USP14 is
            blocked by two surface loops. Binding by ubiquitin induces a
            significant conformational change thereby allowing access of the
            ubiquitin C-terminus to the active site.
REFERENCE   7  (bases 1 to 4972)
  AUTHORS   Dennehey,B.K., Gutches,D.G., McConkey,E.H. and Krauter,K.S.
  TITLE     Inversion, duplication, and changes in gene context are associated
            with human chromosome 18 evolution
  JOURNAL   Genomics 83 (3), 493-501 (2004)
   PUBMED   14962675
REFERENCE   8  (bases 1 to 4972)
  AUTHORS   Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C.
  TITLE     Human and mouse proteases: a comparative genomic approach
  JOURNAL   Nat Rev Genet 4 (7), 544-558 (2003)
   PUBMED   12838346
  REMARK    Review article
REFERENCE   9  (bases 1 to 4972)
  AUTHORS   D'Andrea,A. and Pellman,D.
  TITLE     Deubiquitinating enzymes: a new class of biological regulators
  JOURNAL   Crit Rev Biochem Mol Biol 33 (5), 337-352 (1998)
   PUBMED   9827704
  REMARK    Review article
REFERENCE   10 (bases 1 to 4972)
  AUTHORS   Deshpande,K.L., Seubert,P.H., Tillman,D.M., Farkas,W.R. and
            Katze,J.R.
  TITLE     Cloning and characterization of cDNA encoding the rabbit
            tRNA-guanine transglycosylase 60-kilodalton subunit
  JOURNAL   Arch Biochem Biophys 326 (1), 1-7 (1996)
   PUBMED   8579355
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC003556.1, CA438203.1,
            BU161860.1, AK098497.1 and AP000845.4.
            
            On Nov 22, 2018 this sequence version replaced NM_005151.3.
            
            Summary: This gene encodes a member of the ubiquitin-specific
            processing (UBP) family of proteases that is a deubiquitinating
            enzyme (DUB) with His and Cys domains. This protein is located in
            the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused
            precursors and ubiquitinylated proteins. Mice with a mutation that
            results in reduced expression of the ortholog of this protein are
            retarded for growth, develop severe tremors by 2 to 3 weeks of age
            followed by hindlimb paralysis and death by 6 to 10 weeks of age.
            Alternate transcriptional splice variants, encoding different
            isoforms, have been characterized. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.225761.1,
                                           SRR1803613.242108.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261601.8/ ENSP00000261601.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2955              BC003556.1         5-2959
            2956-3157           CA438203.1         326-527
            3158-3388           BU161860.1         107-337
            3389-4082           AK098497.1         420-1113
            4083-4972           AP000845.4         63453-64342
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.32"
     gene            1..4972
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /note="ubiquitin specific peptidase 14"
                     /db_xref="GeneID:9097"
                     /db_xref="HGNC:HGNC:12612"
                     /db_xref="MIM:607274"
     exon            1..158
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..1627
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /EC_number="3.4.19.12"
                     /note="isoform a is encoded by transcript variant 1;
                     ubiquitin specific protease 14 (tRNA-guanine
                     transglycosylase); tRNA-guanine transglycosylase, 60-kD
                     subunit; ubiquitin carboxyl-terminal hydrolase 14;
                     ubiquitin thiolesterase 14; ubiquitin-specific processing
                     protease 14; deubiquitinating enzyme 14; ubiquitin
                     thioesterase 14; ubiquitin specific peptidase 14
                     (tRNA-guanine transglycosylase)"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 14 isoform
                     a"
                     /protein_id="NP_005142.1"
                     /db_xref="CCDS:CCDS32780.1"
                     /db_xref="GeneID:9097"
                     /db_xref="HGNC:HGNC:12612"
                     /db_xref="MIM:607274"
                     /translation="MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQ
                     KVMVKGGTLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAME
                     LPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLF
                     DSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAI
                     EDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLS
                     CFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYK
                     EKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKK
                     SSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKF
                     DDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ"
     exon            159..304
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            305..337
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            338..442
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            443..546
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            547..605
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            606..736
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            737..817
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            818..903
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1018
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1084
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1177
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1306
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1367
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1475
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     exon            1476..4972
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2198..2203
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /note="hexamer: AATAAA"
     polyA_site      2218
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /note="major polyA site"
     regulatory      2370..2375
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /note="hexamer: AATAAA"
     polyA_site      2391
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
     regulatory      3166..3171
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /note="hexamer: AATAAA"
     polyA_site      3196
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
     regulatory      4952..4957
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
                     /note="hexamer: AAGAAA"
     polyA_site      4972
                     /gene="USP14"
                     /gene_synonym="TGT; Ubp6"
ORIGIN      
        1 gaatgagact cgtcgcaccg aagccgccgc caccaccgcg cctccgcctc ggccgccgcc
       61 gcagctgctc ctggtccccg tccctttgcc gccctcgtca ggcccagctc tcctgcgccg
      121 ccgcctcccg ccgcgccccg ccatgccgct ctactccgtt actgtaaaat ggggaaagga
      181 gaaatttgaa ggtgtagaat tgaatacaga tgaacctcca atggtattca aggctcagct
      241 gtttgcgttg actggagtcc agcctgccag acagaaagtt atggtgaaag gaggaacgct
      301 aaaggatgat gattggggaa acatcaaaat aaaaaatgga atgactctac taatgatggg
      361 gtcagcagat gctcttccag aagaaccctc agccaaaact gtcttcgtag aagacatgac
      421 agaagaacag ttagcatctg ctatggagtt accatgtgga ttgacaaacc ttggtaacac
      481 ttgttacatg aatgccacag ttcagtgtat tcgttctgtg cctgaactca aagatgccct
      541 taaaaggtat gcaggtgcct tgagagcttc aggggaaatg gcttcagcgc agtatattac
      601 tgcagccctt agagatttgt ttgattccat ggataaaact tcttccagta ttccacctat
      661 tattctactg cagtttttgc acatggcttt cccacagttt gccgagaaag gtgaacaagg
      721 acagtatctt caacaggatg ctaatgaatg ttggatacaa atgatgcgag tattgcaaca
      781 gaaattggaa gcaatagagg atgattctgt taaagagaca gactcctcat ctgcatcggc
      841 agcgacacct tctaaaaaga aaagtttaat cgatcagttc ttcggtgttg agtttgaaac
      901 taccatgaaa tgtacagaat ctgaagaaga agaagtcacc aaaggaaagg aaaatcaact
      961 tcagcttagc tgttttatca atcaggaagt caagtatctt tttacaggac ttaaattgcg
     1021 acttcaggaa gaaatcacca aacagtctcc aacgttgcaa agaaatgcct tgtatatcaa
     1081 atcttccaag atcagccggc tgcctgctta cttgaccatt cagatggttc gattttttta
     1141 taaagagaag gaatctgtga atgccaaagt tcttaaggat gttaaatttc ctcttatgtt
     1201 ggatatgtat gaactgtgta caccagaact tcaagagaaa atggtgtctt ttcgatccaa
     1261 attcaaggat ctagaagata aaaaagtgaa tcagcagcca aatacaagtg acaaaaagag
     1321 tagtccccag aaagaagtta agtatgaacc cttttctttt gctgatgata ttggctccaa
     1381 taattgtgga tactatgact tacaagcagt actaacacac cagggaaggt ctagttcttc
     1441 aggtcattat gtatcatggg tgaaaaggaa acaagatgaa tggattaagt ttgatgatga
     1501 caaagtcagc atcgtaacac cagaagatat cttacggctt tctggtggtg gagactggca
     1561 tatcgcttac gttctactct atgggcctcg cagagttgaa ataatggaag aggaaagtga
     1621 acagtaatct tcattttagt atttatgctt agatgtgaaa ataaatgtta tttgttgatc
     1681 atttctataa tccagagctt tagaggaaga cacataggtg ggtttatgtt tcacctcatt
     1741 tggaacaaaa gaggacagaa gcagaccact ctgtgcacca acctaaaaaa ttacagagaa
     1801 gagaaaatta tctttggatt gtgctgccct atataaaggt ggcagaaaga catttttaaa
     1861 aagcttatta tttcttgcat tattttaaaa agttcagagt tgaaatgcct ttcaaccatt
     1921 tccttctgtg gtcatttttc ttgctgcctt tttcacccaa gattcagcag tcagatgttt
     1981 actgcacacc tattacctat tatttgctgt tcttgcatgg ttcaaaccac cattctgtag
     2041 ccacccatcc tttgccttat ctaacaaaca tttttccagg aaggtggaaa aggaagtgtt
     2101 gctctcattg tgtgactcag tgctgctgtc catcccatgg aaacatgggc acaatcaagt
     2161 atttgtccag cctattgcag gcttttcctg actttaaaat aaattgtgat caataatagt
     2221 acctttgatt atacatttat tattgtgtct ctctctgatg tactgtggat tgtacattta
     2281 actttggaat ggctttgtaa taatcagtct taagaaaatg ttgacaagct ctggttgctt
     2341 atttttagaa aatgaggaca tttaataata ataaaaaaaa agggattaat agcttttgac
     2401 ctcaagtctt ttgtcttctg agtgttggag cttggctgaa gacatgttta atactgtaca
     2461 atttctgaag atggttatta acactgtgct gttaagcatc catttaaaaa tatttgttat
     2521 cttctttgcc tgcctgtatt ttaaaaagaa atacaaagtt gtaaaagtaa tggatttctt
     2581 tggatgctga atgcagtgat gttatcagtc agattctttc cttggctcag ttgtgtttgt
     2641 atttcctttc ttaattcccc aaatcttggc tgggcacggt ggctcacgcc tgtaatccca
     2701 gcactttggg aggctgaggc aggtggttca ctggaggttg agttcgagac cagcctggcc
     2761 acatggtgaa accctgtctc tactaaagat ataaaaatta gctggatgtg gtggtgcaca
     2821 cctgtgatcc cagctacttg ggaggctgag gtgggagaat tgcttgaacc tggaaggcag
     2881 aagttgcagt gagctgagat cacaccactg cactccagcc tgggtgacag agagactctg
     2941 tctcaaaaaa aaaaagaaaa aattcccaaa tcttaatgtc tgtttcagaa ttttagtgat
     3001 tttaaagtga tagtagaaaa taccaagcat atgaggtatt tcaatatgat aggcttctta
     3061 cattttaata gttactcatc tttgttccag gacccttgac tgatgctagg gaaaggataa
     3121 agcatagaat accagccagt ttggctctga ttttgagtta tttataataa agtttgggga
     3181 ttatcataac atctcatatc tttgtgtatg ttgttttaat gctcggcaga gcacaacact
     3241 gatgataact attaaaaata gaaaataaca gccattttac tttcaaatct gatgtgatgc
     3301 aatctgtgaa cactaggagg ggataaaata agcattgcag ggaaacttat ttgtaaatag
     3361 cttcctcacc taaactttga cttggcaatc aatactttct actttgtgga ggggattaag
     3421 aatagaatgg gcaggatgag accatgaaaa ttataatgat tcatgtatat ttgtatgatg
     3481 cttgtaactt tgcaaagtct tttttattca ttgtctcatt tggtcagaac aattttattg
     3541 aggacatgaa tttaacaatt agaatttaga ttttctgact gtagctacta aatgttgtag
     3601 ctactagacc aggtgagcta ggtttagagc aaagtatata agccttgtat ggactatatt
     3661 agaattttaa aaattgtaat taattcctta tcatcattat aaaaagcttg atttttttat
     3721 ttgatctaaa aaagcattat tccaaagaat tttttcaagt agaaatggga gttagttata
     3781 ccagtgttgt ttttaactaa taaggctatt ttagaattca gccttgcctg taaggtcttt
     3841 gagaagggag aggagtaggc caaaaaaaaa aaagtcttga ttcctgaatg tgccttatat
     3901 gctgtgccac tcacaccgag gccatcatct caacattaga gttgtgctag attactgctt
     3961 gactgactag ctaggttagg cctaagagtg tttaccgaat aatctgcact cactcagtag
     4021 cccttcttca ggattaaaaa cgatgatgac ctcatcttag agccctaaag ggaaatgctt
     4081 atgtgggaac aagaaaagtc ggtggtactg tcttctgcta ctactactta gtgctttgct
     4141 gtgtataggg tgtgttcaca tatatctcag ttgaatgtca caagaatccc tgaagtgatt
     4201 aaaagtcttg agaggtcagg caatttttgt aaggctagaa ggaaaagtga ggttttagac
     4261 ttcatttctt ttaaagttgg caaacaaaca ttttctgtaa tggacaagat agatagatta
     4321 gatagataga tagatagata gatgatgatt gattgatgat tgatagtaaa ttatttcagg
     4381 ctttgcaggc tataaagtgc tgcagctcct tagctttgct gttgttatag tgtagctgga
     4441 gccatagaca gttagttagt tagttatgag tgagcacagc tgggtgccag tacaatttta
     4501 atgatcaaga ctcgtggcag gcagaacaaa ccatcatttg ttgatcctct gcttgaaagc
     4561 acgaataagt gagaacagag cagtcatatg gtttcagaaa gtgttattct gggatttcag
     4621 gataaatttt gtaaaactac taacaaacaa cacacaggaa actaatttgg tataagaaac
     4681 gacatatccc taaatagtgc ttatttaact tcattataaa tacatctact taacaaaaaa
     4741 atatattttg tgatcttgag gtcacttcgt ttaggtcatt atctcaaccc aacctcccca
     4801 aactctggtt tgagccaata tgtgttctat tgttctcaga gcaccagccg actgtacaac
     4861 aattgttata aaaatgttta ttgtttacca aaaccagtgg acctcttatc aaatgctgct
     4921 tggtaacaaa atctatcaca gttttaataa aaagaaaaaa aaaagaagct aa
//
