LOCUS       NM_003715               4123 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens USO1 vesicle transport factor (USO1), transcript
            variant 2, mRNA.
ACCESSION   NM_003715
VERSION     NM_003715.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Kim,D.K., Weller,B., Lin,C.W., Sheykhkarimli,D., Knapp,J.J.,
            Dugied,G., Zanzoni,A., Pons,C., Tofaute,M.J., Maseko,S.B.,
            Spirohn,K., Laval,F., Lambourne,L., Kishore,N., Rayhan,A.,
            Sauer,M., Young,V., Halder,H., la Rosa,N.M., Pogoutse,O.,
            Strobel,A., Schwehn,P., Li,R., Rothballer,S.T., Altmann,M.,
            Cassonnet,P., Cote,A.G., Vergara,L.E., Hazelwood,I., Liu,B.B.,
            Nguyen,M., Pandiarajan,R., Dohai,B., Coloma,P.A.R., Poirson,J.,
            Giuliana,P., Willems,L., Taipale,M., Jacob,Y., Hao,T., Hill,D.E.,
            Brun,C., Twizere,J.C., Krappmann,D., Heinig,M., Falter,C., Aloy,P.,
            Demeret,C., Vidal,M., Calderwood,M.A., Roth,F.P. and
            Falter-Braun,P.
  TITLE     A proteome-scale map of the SARS-CoV-2-human contactome
  JOURNAL   Nat Biotechnol 41 (1), 140-149 (2023)
   PUBMED   36217029
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Yoon,S., Choi,J.H., Shah,M., Kwon,S.M., Yang,J., Park,Y.N.,
            Wang,H.J. and Woo,H.G.
  TITLE     USO1 isoforms differentially promote liver cancer progression by
            dysregulating the ER-Golgi network
  JOURNAL   Carcinogenesis 42 (9), 1208-1220 (2021)
   PUBMED   34293111
  REMARK    GeneRIF: USO1 isoforms differentially promote liver cancer
            progression by dysregulating the ER-Golgi network.
REFERENCE   3  (bases 1 to 4123)
  AUTHORS   Jaiswal,A.K., Truong,H., Tran,T.M., Lin,T.L., Casero,D.,
            Alberti,M.O. and Rao,D.S.
  TITLE     Focused CRISPR-Cas9 genetic screening reveals USO1 as a
            vulnerability in B-cell acute lymphoblastic leukemia
  JOURNAL   Sci Rep 11 (1), 13158 (2021)
   PUBMED   34162911
  REMARK    GeneRIF: Focused CRISPR-Cas9 genetic screening reveals USO1 as a
            vulnerability in B-cell acute lymphoblastic leukemia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4123)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 4123)
  AUTHORS   Jin,Y. and Dai,Z.
  TITLE     USO1 promotes tumor progression via activating Erk pathway in
            multiple myeloma cells
  JOURNAL   Biomed Pharmacother 78, 264-271 (2016)
   PUBMED   26898451
  REMARK    GeneRIF: USO1 gene may be a promising target for the therapy of
            human multiple myeloma and its diagnosis marker.
REFERENCE   6  (bases 1 to 4123)
  AUTHORS   Nelson,D.S., Alvarez,C., Gao,Y.S., Garcia-Mata,R., Fialkowski,E.
            and Sztul,E.
  TITLE     The membrane transport factor TAP/p115 cycles between the Golgi and
            earlier secretory compartments and contains distinct domains
            required for its localization and function
  JOURNAL   J Cell Biol 143 (2), 319-331 (1998)
   PUBMED   9786945
REFERENCE   7  (bases 1 to 4123)
  AUTHORS   Barr,F.A., Nakamura,N. and Warren,G.
  TITLE     Mapping the interaction between GRASP65 and GM130, components of a
            protein complex involved in the stacking of Golgi cisternae
  JOURNAL   EMBO J 17 (12), 3258-3268 (1998)
   PUBMED   9628863
REFERENCE   8  (bases 1 to 4123)
  AUTHORS   Sohda,M., Misumi,Y., Yano,A., Takami,N. and Ikehara,Y.
  TITLE     Phosphorylation of the vesicle docking protein p115 regulates its
            association with the Golgi membrane
  JOURNAL   J Biol Chem 273 (9), 5385-5388 (1998)
   PUBMED   9478999
REFERENCE   9  (bases 1 to 4123)
  AUTHORS   Nakamura,N., Lowe,M., Levine,T.P., Rabouille,C. and Warren,G.
  TITLE     The vesicle docking protein p115 binds GM130, a cis-Golgi matrix
            protein, in a mitotically regulated manner
  JOURNAL   Cell 89 (3), 445-455 (1997)
   PUBMED   9150144
REFERENCE   10 (bases 1 to 4123)
  AUTHORS   Waters,M.G., Clary,D.O. and Rothman,J.E.
  TITLE     A novel 115-kD peripheral membrane protein is required for
            intercisternal transport in the Golgi stack
  JOURNAL   J Cell Biol 118 (5), 1015-1026 (1992)
   PUBMED   1512287
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC104828.4, AEKP01043041.1 and
            AC110615.3.
            
            On Feb 27, 2020 this sequence version replaced NM_003715.3.
            
            Summary: The protein encoded by this gene is a peripheral membrane
            protein which recycles between the cytosol and the Golgi apparatus
            during interphase. It is regulated by phosphorylation:
            dephosphorylated protein associates with the Golgi membrane and
            dissociates from the membrane upon phosphorylation. Ras-associated
            protein 1 recruits this protein to coat protein complex II (COPII)
            vesicles during budding from the endoplasmic reticulum, where it
            interacts with a set of COPII vesicle-associated SNAREs to form a
            cis-SNARE complex that promotes targeting to the Golgi apparatus.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Feb 2014].
            
            Transcript Variant: This variant (2) lacks two exons in the central
            coding region, but maintains the reading frame, compared to variant
            1. The encoded isoform (2) is shorter, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074968.3843213.1, D86326.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000514213.7/ ENSP00000444850.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-309               AC104828.4         135687-135995
            310-396             AEKP01043041.1     3629-3715           c
            397-461             AEKP01043041.1     3484-3548           c
            462-538             AC104828.4         164576-164652
            539-639             AC104828.4         177518-177618
            640-742             AC104828.4         177901-178003
            743-798             AC104828.4         178161-178216
            799-919             AC110615.3         2266-2386
            920-1098            AC110615.3         10270-10448
            1099-1239           AC110615.3         14652-14792
            1240-1328           AC110615.3         17740-17828
            1329-1483           AC110615.3         18233-18387
            1484-1695           AC110615.3         21280-21491
            1696-1806           AC110615.3         27212-27322
            1807-1925           AC110615.3         27941-28059
            1926-2107           AC110615.3         28208-28389
            2108-2229           AC110615.3         28669-28790
            2230-2368           AC110615.3         31724-31862
            2369-2532           AC110615.3         32730-32893
            2533-2619           AC110615.3         34076-34162
            2620-2718           AC110615.3         36543-36641
            2719-2826           AC110615.3         38022-38129
            2827-3042           AC110615.3         39750-39965
            3043-4123           AC110615.3         40796-41876
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q21.1"
     gene            1..4123
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /note="USO1 vesicle transport factor"
                     /db_xref="GeneID:8615"
                     /db_xref="HGNC:HGNC:30904"
                     /db_xref="MIM:603344"
     exon            1..309
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     CDS             244..3132
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /note="isoform 2 is encoded by transcript variant 2;
                     vesicle docking protein p115; transcytosis associated
                     protein; general vesicular transport factor p115; USO1
                     vesicle docking protein homolog"
                     /codon_start=1
                     /product="general vesicular transport factor p115 isoform
                     2"
                     /protein_id="NP_003706.2"
                     /db_xref="CCDS:CCDS75144.1"
                     /db_xref="GeneID:8615"
                     /db_xref="HGNC:HGNC:30904"
                     /db_xref="MIM:603344"
                     /translation="MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVR
                     ALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVEENS
                     TRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQI
                     ILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIIS
                     EEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQK
                     VTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETI
                     NTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCF
                     LYKNQKGQGEIVSTLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQE
                     NATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTRVGLLMLLCTWLSNCPIA
                     VTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQ
                     LIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVI
                     TKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQ
                     LQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIE
                     ELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLK
                     SQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQE
                     TKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLA
                     DQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI"
     exon            310..396
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            397..461
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            462..538
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            539..639
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            640..742
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            743..798
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            799..919
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1098
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1239
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1328
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1483
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1695
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1806
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1925
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2107
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2229
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            2230..2368
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..2532
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2619
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            2620..2718
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            2719..2826
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            2827..3042
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     exon            3043..4123
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4032..4037
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /note="hexamer: AATAGA"
     polyA_site      4050
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /note="major polyA site"
     regulatory      4100..4105
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
                     /note="hexamer: AATAAA"
     polyA_site      4123
                     /gene="USO1"
                     /gene_synonym="P115; TAP; VDP"
ORIGIN      
        1 cccttttgcc ttcaaccttc gagccgccac gtaatgccac gtccccgcgc atgcgcatct
       61 tggccgctgc tggcggctgt ttccgggctt agagggctgg agtggccgcc gagttggagg
      121 cggtggtggc agcagtagga gtgtgtagag tgcgggattg gggcccaggc cctgcggagg
      181 gcgggggaag ttgtcttctt ttttttccgg aggggccggt aaacctggtg gctgaacggc
      241 aagatgaatt tcctccgcgg ggtaatgggg ggtcagagtg ccggacccca gcacacagaa
      301 gccgagacga ttcaaaagct ttgtgacaga gtagcttcat ctactttatt ggatgatcga
      361 agaaatgctg ttcgtgctct caaatcatta tctaagaaat accgcttgga agtgggtata
      421 caagctatgg aacatcttat tcatgtttta caaacagatc gttcagattc tgaaataata
      481 ggttatgctt tggacacact atataatata atatctaatg aagaagaaga agaagtagaa
      541 gaaaattcca caagacagag tgaagatttg ggaagccaat ttacagaaat tttcattaag
      601 cagcaggaaa atgtcactct tctgttatct ttattggagg agtttgattt ccatgtccgc
      661 tggcctggtg tgaagcttct tacttctctt ttaaaacaac tagggcctca ggtgcaacaa
      721 attattttag tcagtcctat gggtgtttca agattgatgg acttactagc ggattccagg
      781 gaagttatac gtaatgatgg cgtcttacta ctgcaggcac taacaagaag caatggtgca
      841 atccagaaaa ttgttgcttt tgaaaatgct ttcgagagac tactggacat tatttcagag
      901 gaggggaaca gtgatggagg tatagtagtt gaagattgtt tgattttgct ccaaaactta
      961 ttaaaaaaca acaactccaa tcaaaatttt tttaaagaag gctcatatat tcaacgtatg
     1021 aaaccttggt ttgaagttgg agatgaaaat tctggctggt ctgcacagaa agtgaccaat
     1081 ctacatctaa tgctacagct tgttcgtgta ttggtatctc ccaccaaccc tcctggtgct
     1141 accagtagct gccagaaggc tatgttccag tgtgggttat tgcagcagct ttgtactatc
     1201 ctaatggcta ctggggttcc tgctgatatc ctgactgaga ccataaatac tgtatcagaa
     1261 gttattcgag gctgccaagt aaaccaagac tactttgcat ctgtaaatgc accttcaaac
     1321 ccaccaagac cggcaattgt agtacttctc atgtccatgg ttaatgaaag gcagccattt
     1381 gttttgcgct gtgctgttct ctattgtttc cagtgtttct tgtataaaaa ccaaaaagga
     1441 caaggagaaa tcgtgtcaac acttttacct tctaccattg atgcaacagg taattcagtt
     1501 tcagctggcc agttattatg tggaggtttg ttttctactg attcactttc aaactggtgt
     1561 gctgctgtgg cccttgccca tgcgttgcaa gaaaatgcca cccagaaaga acagttgctc
     1621 agggttcaac ttgctacaag tattggcaac cctccagttt ctttacttca acagtgcacc
     1681 aatattcttt cacagggaag caaaatacaa acaagagttg gattattaat gttgctttgt
     1741 acctggctaa gcaattgtcc cattgcagta acgcattttc ttcacaattc agccaatgtt
     1801 ccatttctta caggacaaat tgcagaaaat cttggagaag aagagcagtt ggtccaaggc
     1861 ttatgtgccc ttttgttggg catttcgatt tatttcaatg ataactcact tgagagctac
     1921 atgaaagaga agctaaaaca actgattgag aagaggattg gcaaagagaa tttcatagag
     1981 aaactaggat ttattagcaa acatgagttg tattccagag catctcagaa accccagcca
     2041 aactttccca gtccagaata catgatattt gatcatgagt ttacgaagct ggtaaaagaa
     2101 cttgaaggtg ttataactaa ggctatttat aagtccagtg aagaagataa aaaagaagaa
     2161 gaggtgaaaa aaacattaga acagcatgac aatattgtga ctcactacaa aaatatgatt
     2221 cgagagcagg atctccaact tgaggaatta aggcagcagg tttctacatt aaaatgtcaa
     2281 aatgaacagc tccagacggc agtcacacag caagtatcac agatccagca gcacaaagac
     2341 cagtataatc ttcttaaaat acagctagga aaagacaatc agcatcaagg ttcttacagt
     2401 gagggggctc agatgaatgg cattcagcca gaagaaattg gtagattgcg agaagagata
     2461 gaagaattaa aacgtaatca ggaactttta caaagccagc tgactgaaaa ggactctatg
     2521 attgaaaata tgaaatcttc ccaaacatct ggcacaaatg aacagtcttc agcaatagtt
     2581 tcagctagag attctgaaca agttgcagaa ttaaaacagg aactggcaac tttaaagtct
     2641 cagttaaact cacaatctgt ggagatcacc aaactacaga cagaaaagca ggaactgtta
     2701 cagaaaacag aagcgtttgc aaaatcagtt gaggtacaag gagagaccga gactataata
     2761 gccaccaaaa ctactgatgt agaaggaaga ctgtcagcat tattacaaga gaccaaagag
     2821 ttaaagaatg aaattaaagc tctgtctgag gaaagaactg ccattaaaga gcagctggat
     2881 tcatctaata gtaccattgc cattttacaa actgagaaag acaaactaga gttggaaatt
     2941 acagattcta aaaaagaaca agatgatctc ttggtgctct tggccgatca agatcagaaa
     3001 atactgtcat tgaagaataa actcaaggat cttggtcatc cagttgaaga agaggatgaa
     3061 cttgaatctg gagaccaaga ggatgaggat gatgaaagtg aagatcctgg caaggatcta
     3121 gatcatatct agttttcaaa tctctaggaa caatagagat tatatcataa ctctgaagat
     3181 ggactctaaa ctttggttta cctccatgga aaataaagat ttagaaacca ggtggaaaac
     3241 attcactatt aagactattg atatattttt gtaatgttgc cacccatgtt gaaaacctta
     3301 aaactgaatt tatgtagaac acacatcttt tatttaaatc catctgaact gtcccaaaat
     3361 gtaatttgca aagagcttca aacaccaaaa atataccatt ttaaggcatg tggtgacaat
     3421 gctatttgcc ttagttcatt cgttgtttac tttgcaaaat ttgtgacttt taagtactaa
     3481 cacatttgat tgaatgtgta ttgaattgga attattattt ctaaattcag catttgagtc
     3541 cttagttgat gattttcttt taaagaaatt agtttattta aaagcaagat agcctgggtt
     3601 gggagttagt gcatatcatc agccctccat ctaatgtgat tgctaagatg tgcaggatag
     3661 gacagcttag aatgagtgcc ttgacaccta catatggctg cattccatac ctcagtgaag
     3721 aagccgaagg atgctgacac acagtgatgt ggagggcatt ccggagaaac ttttgcaaca
     3781 gtgtattaat gtgattggtt ttctttttgt tttaagctaa acatctaaaa caaatttcaa
     3841 gttaacagtt catacaactc agatttagta gtattgttta tcataaaaca tattatgttc
     3901 ctagttttaa aaagacccaa agtatgatta atataattta ttggcatttt tgctgaatag
     3961 gtttaagtca gataatagat tttaaaaaag caaatgaggc aatgtgtcaa aatttaatgt
     4021 ttcataataa aaatagatac gttgacttaa tccttggtca tggttccctt tgtttacatc
     4081 ttaaaagtat tttattctta ataaaagatg aggcaagtga aaa
//
