LOCUS       NM_153676               3241 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens USH1 protein network component harmonin (USH1C),
            transcript variant b3, mRNA.
ACCESSION   NM_153676
VERSION     NM_153676.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Nagel-Wolfrum,K., Fadl,B.R., Becker,M.M., Wunderlich,K.A.,
            Schafer,J., Sturm,D., Fritze,J., Gur,B., Kaplan,L., Andreani,T.,
            Goldmann,T., Brooks,M., Starostik,M.R., Lokhande,A., Apel,M.,
            Fath,K.R., Stingl,K., Kohl,S., DeAngelis,M.M.,
            Schlotzer-Schrehardt,U., Kim,I.K., Owen,L.A., Vetter,J.M.,
            Pfeiffer,N., Andrade-Navarro,M.A., Grosche,A., Swaroop,A. and
            Wolfrum,U.
  TITLE     Expression and subcellular localization of USH1C/harmonin in human
            retina provides insights into pathomechanisms and therapy
  JOURNAL   Hum Mol Genet 32 (3), 431-449 (2023)
   PUBMED   35997788
  REMARK    GeneRIF: Expression and subcellular localization of USH1C/harmonin
            in human retina provides insights into pathomechanisms and therapy.
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3241)
  AUTHORS   Noman,M., Bukhari,S.A., Rehman,S., Qasim,M., Ali,M., Riazuddin,S.
            and Ahmed,Z.M.
  TITLE     Identification and computational analysis of USH1C, and SLC26A4
            variants in Pakistani families with prelingual hearing loss
  JOURNAL   Mol Biol Rep 47 (12), 9987-9993 (2020)
   PUBMED   33231815
  REMARK    GeneRIF: Identification and computational analysis of USH1C, and
            SLC26A4 variants in Pakistani families with prelingual hearing
            loss.
REFERENCE   4  (bases 1 to 3241)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 3241)
  AUTHORS   Baux,D., Faugere,V., Larrieu,L., Le Guedard-Mereuze,S., Hamroun,D.,
            Beroud,C., Malcolm,S., Claustres,M. and Roux,A.F.
  TITLE     UMD-USHbases: a comprehensive set of databases to record and
            analyse pathogenic mutations and unclassified variants in seven
            Usher syndrome causing genes
  JOURNAL   Hum Mutat 29 (8), E76-E87 (2008)
   PUBMED   18484607
REFERENCE   6  (bases 1 to 3241)
  AUTHORS   Saouda,M., Mansour,A., Bou Moglabey,Y., El Zir,E., Mustapha,M.,
            Chaib,H., Nehme,A., Megarbane,A., Loiselet,J., Petit,C. and Slim,R.
  TITLE     The Usher syndrome in the Lebanese population and further
            refinement of the USH2A candidate region
  JOURNAL   Hum Genet 103 (2), 193-198 (1998)
   PUBMED   9760205
REFERENCE   7  (bases 1 to 3241)
  AUTHORS   Jain,P.K., Lalwani,A.K., Li,X.C., Singleton,T.L., Smith,T.N.,
            Chen,A., Deshmukh,D., Verma,I.C., Smith,R.J. and Wilcox,E.R.
  TITLE     A gene for recessive nonsyndromic sensorineural deafness (DFNB18)
            maps to the chromosomal region 11p14-p15.1 containing the Usher
            syndrome type 1C gene
  JOURNAL   Genomics 50 (2), 290-292 (1998)
   PUBMED   9653658
REFERENCE   8  (bases 1 to 3241)
  AUTHORS   Scanlan,M.J., Chen,Y.T., Williamson,B., Gure,A.O., Stockert,E.,
            Gordan,J.D., Tureci,O., Sahin,U., Pfreundschuh,M. and Old,L.J.
  TITLE     Characterization of human colon cancer antigens recognized by
            autologous antibodies
  JOURNAL   Int J Cancer 76 (5), 652-658 (1998)
   PUBMED   9610721
REFERENCE   9  (bases 1 to 3241)
  AUTHORS   Shearer,A.E., Hildebrand,M.S., Odell,A.M. and Smith,R.J.H.
  TITLE     Genetic Hearing Loss Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301607
REFERENCE   10 (bases 1 to 3241)
  AUTHORS   Koenekoop,R.K., Arriaga,M.A., Trzupek,K.M. and Lentz,J.J.
  TITLE     Usher Syndrome Type I
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301442
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124799.5 and KF455327.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_153676.3.
            
            Summary: This gene encodes a scaffold protein that functions in the
            assembly of Usher protein complexes. The protein contains PDZ
            domains, a coiled-coil region with a bipartite nuclear localization
            signal and a PEST degradation sequence. Defects in this gene are
            the cause of Usher syndrome type 1C and non-syndromic sensorineural
            deafness autosomal recessive type 18. Multiple transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Mar 2009].
            
            Transcript Variant: This variant (b3) encodes the longest isoform
            (b3).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BK000147.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000005226.12/ ENSP00000005226.7
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               AC124799.5         55148-55292         c
            146-213             AC124799.5         44131-44198         c
            214-357             AC124799.5         42275-42418         c
            358-496             AC124799.5         42030-42168         c
            497-605             KF455327.1         35-143
            606-630             AC124799.5         37892-37916         c
            631-688             AC124799.5         37629-37686         c
            689-783             AC124799.5         37223-37317         c
            784-868             AC124799.5         35327-35411         c
            869-928             AC124799.5         34295-34354         c
            929-985             AC124799.5         34087-34143         c
            986-1128            AC124799.5         33660-33802         c
            1129-1194           AC124799.5         32222-32287         c
            1195-1319           AC124799.5         31746-31870         c
            1320-1369           AC124799.5         27117-27166         c
            1370-1522           AC124799.5         22778-22930         c
            1523-1639           AC124799.5         21281-21397         c
            1640-2122           AC124799.5         20232-20714         c
            2123-2242           AC124799.5         16706-16825         c
            2243-2293           AC124799.5         15523-15573         c
            2294-2335           AC124799.5         12815-12856         c
            2336-2389           AC124799.5         12358-12411         c
            2390-2489           AC124799.5         11927-12026         c
            2490-2599           AC124799.5         9038-9147           c
            2600-2655           AC124799.5         7634-7689           c
            2656-2764           AC124799.5         6445-6553           c
            2765-3241           AC124799.5         4776-5252           c
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.1"
     gene            1..3241
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /note="USH1 protein network component harmonin"
                     /db_xref="GeneID:10083"
                     /db_xref="HGNC:HGNC:12597"
                     /db_xref="MIM:605242"
     exon            1..145
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..2809
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /note="isoform b3 is encoded by transcript variant b3;
                     harmonin; antigen NY-CO-38/NY-CO-37; usher syndrome
                     type-1C protein; renal carcinoma antigen NY-REN-3;
                     autoimmune enteropathy-related antigen AIE-75; Usher
                     syndrome 1C (autosomal recessive, severe)"
                     /codon_start=1
                     /product="harmonin isoform b3"
                     /protein_id="NP_710142.1"
                     /db_xref="CCDS:CCDS7825.1"
                     /db_xref="GeneID:10083"
                     /db_xref="HGNC:HGNC:12597"
                     /db_xref="MIM:605242"
                     /translation="MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVG
                     DLKLVINEPSRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSV
                     RGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTV
                     SIKVRHIGLIPVKSSPDEPLTWQYVDQFVSESGGVRGSLGSPGNRENKEKKVFISLVG
                     SRGLGCSISSGPIQKPGIFISHVKPGSLSAEVGLEIGDQIVEVNGVDFSNLDHKEAVN
                     VLKSSRSLTISIVAAAGRELFMTDRERLAEARQRELQRQELLMQKRLAMESNKILQEQ
                     QEMERQRRKEIAQKAAEENERYRKEMEQIVEEEEKFKKQWEEDWGSKEQLLLPKTITA
                     EVHPVPLRKPKSFGWFYRYDGKFPTIRKKGKDKKKAKYGSLQDLRKNKKELEFEQKLY
                     KEKEEMLEKEKQLKINRLAQEVSETEREDLEESEKIQYWVERLCQTRLEQISSADNEI
                     SEMTTGPPPPPPSVSPLAPPLRRFAGGLHLHTTDLDDIPLDMFYYPPKTPSALPVMPH
                     PPPSNPPHKVPAPPVLPLSGHVSASSSPWVQRTPPPIPIPPPPSVPTQDLTPTRPLPS
                     ALEEALSNHPFRTGDTGNPVEDWEAKNHSGKPTNSPVPEQSFPPTPKTFCPSPQPPRG
                     PGVSTISKPVMVHQEPNFIYRPAVKSEVLPQEMLKRMVVYQTAFRQDFRKYEEGFDPY
                     SMFTPEQIMGKDVRLLRIKKEGSLDLALEGGVDSPIGKVVVSAVYERGAAERHGGIVK
                     GDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELASLPSSV
                     AESPQPVRKLLEDRAAVHRHGFLLQLEPTDLLLKSKRGNQIHR"
     exon            146..213
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            214..357
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            358..496
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            497..605
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            606..630
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            631..688
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            689..783
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            784..868
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            869..928
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            929..985
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1128
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1194
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1319
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1369
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1522
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1639
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            1640..2122
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            2123..2242
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2293
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2335
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            2336..2389
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            2390..2489
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2599
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            2600..2655
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            2656..2764
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     exon            2765..3241
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3220..3225
                     /regulatory_class="polyA_signal_sequence"
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /note="hexamer: AATAAA"
     polyA_site      3241
                     /gene="USH1C"
                     /gene_synonym="AIE-75; DFNB18; DFNB18A; NY-CO-37;
                     NY-CO-38; PDZ-45; PDZ-73; PDZ-73/NY-CO-38; PDZ73; PDZD7C;
                     ush1cpst"
                     /note="major polyA site"
ORIGIN      
        1 agctccgagg gcggctggcc cggtcgcggt cgcggctctt tccagctcct ggcagccggg
       61 cacccgaagg aacgggtcgt gcaacgacgc agctggacct ggcccagcca tggaccgaaa
      121 agtggcccga gaattccggc ataaggtgga ttttctgatt gaaaatgatg cagagaagga
      181 ctatctctat gatgtgctgc gaatgtacca ccagaccatg gacgtggccg tgctcgtggg
      241 agacctgaag ctggtcatca atgaacccag ccgtctgcct ctgtttgatg ccattcggcc
      301 gctgatccca ctgaagcacc aggtggaata tgatcagctg accccccggc gctccaggaa
      361 gctgaaggag gtgcgtctgg accgtctgca ccccgaaggc ctcggcctga gtgtgcgtgg
      421 tggcctggag tttggctgtg ggctcttcat ctcccacctc atcaaaggcg gtcaggcaga
      481 cagcgtcggg ctccaggtag gggacgagat cgtccggatc aatggatatt ccatctcctc
      541 ctgtacccat gaggaggtca tcaacctcat tcgaaccaag aaaactgtgt ccatcaaagt
      601 gagacacatc ggcctgatcc ccgtgaaaag ctctcctgat gagcccctca cttggcagta
      661 tgtggatcag tttgtgtcgg aatctggggg cgtgcgaggc agcctgggct cccctggaaa
      721 tcgggaaaac aaggagaaga aggtcttcat cagcctggta ggctcccgag gccttggctg
      781 cagcatttcc agcggcccca tccagaagcc tggcatcttt atcagccatg tgaaacctgg
      841 ctccctgtct gctgaggtgg gattggagat aggggaccag attgtcgaag tcaatggcgt
      901 cgacttctct aacctggatc acaaggaggc tgtaaatgtg ctgaagagta gccgcagcct
      961 gaccatctcc attgtagctg cagctggccg ggagctgttc atgacagacc gggagcggct
     1021 ggcagaggcg cggcagcgtg agctgcagcg gcaggagctt ctcatgcaga agcggctggc
     1081 gatggagtcc aacaagatcc tccaggagca gcaggagatg gagcggcaaa ggagaaaaga
     1141 aattgcccag aaggcagcag aggaaaatga gagataccgg aaggagatgg aacagattgt
     1201 agaggaggaa gagaagttta agaagcaatg ggaagaagac tggggctcaa aggaacagct
     1261 actcttgcct aaaaccatca ctgctgaggt acacccagta ccccttcgca agccaaagtc
     1321 ttttggatgg ttttatcgtt acgatggcaa attcccaacc atccggaaga aaggaaaaga
     1381 taagaagaaa gccaagtatg gcagcctgca ggacttgaga aagaataaga aagaactgga
     1441 gtttgagcaa aagctttaca aagagaaaga ggaaatgctg gagaaggaaa agcagctaaa
     1501 gatcaaccgg ctggcccagg aggtgtctga gacagagcgg gaagaccttg aagaatcgga
     1561 aaagattcaa tattgggtgg agaggctctg tcaaacgcgc ctcgagcaga tttcctctgc
     1621 tgataatgag atttcagaga tgaccacagg gcccccgcct cccccgcctt ctgtgtctcc
     1681 cctggcccca cccttgagac gcttcgcagg cggactgcac ctgcacacca ctgacctgga
     1741 cgacatccct ttggacatgt tctactatcc ccccaagact ccctctgcct tgcctgtgat
     1801 gccccaccct ccaccctcca acccacccca caaggtcccg gcgccccctg tccttccctt
     1861 atctggccat gtgagcgcct catcctctcc atgggtgcag cgcactccac cccccattcc
     1921 catccctccc ccgccatccg ttcccaccca agacctcact cccacccgcc cactgccctc
     1981 ggcgctggaa gaagcactga gcaaccatcc cttccgcact ggggacacag gcaatccagt
     2041 ggaggactgg gaggcaaaga accacagtgg gaagcccact aactcccctg tccctgaaca
     2101 gagcttccca cccaccccaa agacattttg cccaagccca cagcctccac gaggccctgg
     2161 cgtgtccacc atctccaaac ctgtcatggt ccaccaggag cccaatttca tctacaggcc
     2221 agctgtgaaa tctgaagttc tgccacagga gatgttgaag aggatggtgg tttatcagac
     2281 agcattcaga caagatttcc ggaaatatga ggaaggcttt gacccctact ctatgttcac
     2341 cccagagcag atcatgggga aggatgtccg gctcctacgc atcaagaagg agggatcctt
     2401 agacctggcc ctggaaggcg gtgtggactc ccccattggg aaggtggtcg tttctgctgt
     2461 gtatgagcgg ggagctgctg agcggcatgg tggcattgtg aaaggggacg agatcatggc
     2521 aatcaacggc aagattgtga cagactacac cctggctgag gctgaggctg ccctgcagaa
     2581 ggcctggaat cagggcgggg actggatcga ccttgtggtt gccgtctgcc ccccaaagga
     2641 gtatgacgat gagctagctt ctcttccctc ctccgtagct gaaagccccc aaccggtccg
     2701 aaagctcctt gaagaccgtg ctgccgtgca cagacacggg ttcctcctgc agctggagcc
     2761 cacggacctt cttctgaagt ccaaaagggg aaaccaaatt caccgttagg aaacagtgag
     2821 ctccggcccc acctcgtgaa cacaaagcct cggatcagcc ttgagagagg ccacactaca
     2881 cacaccagat ggcatccttg ggacctgaat ctatcaccca ggaatctcaa actccctttg
     2941 gccctgaacc agggccagat aaggaacagc tcgggccact cttctgaagg ccaacgtgga
     3001 ggaaagggag cagccagcca tttgggagaa gatctcaagg atccagactc tcattccttt
     3061 cctctggccc agtgaatttg gtctctccca gctctggggg actccttcct tgaaccctaa
     3121 taagacccca ctggagtctc tctctctcca tccctctcct ctgccctctg ctctaattgc
     3181 tgccaggatt gtcactccaa accttactct gagctcatta ataaaataga tttattttcc
     3241 a
//
