LOCUS       NM_001135570            1702 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ubiquinol-cytochrome c reductase complex assembly
            factor 6 (UQCC6), mRNA.
ACCESSION   NM_001135570 XR_042054 XR_042055 XR_042056
VERSION     NM_001135570.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Wang,Y., Shi,Y., Li,W., Han,X., Lin,X., Liu,D., Lin,Y. and Shen,L.
  TITLE     Knockdown of BRAWNIN minimally affect mitochondrial complex III
            assembly in human cells
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (1), 119601 (2024)
   PUBMED   37769950
  REMARK    GeneRIF: Knockdown of BRAWNIN minimally affect mitochondrial
            complex III assembly in human cells.
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Dennerlein,S., Poerschke,S., Oeljeklaus,S., Wang,C.,
            Richter-Dennerlein,R., Sattmann,J., Bauermeister,D., Hanitsch,E.,
            Stoldt,S., Langer,T., Jakobs,S., Warscheid,B. and Rehling,P.
  TITLE     Defining the interactome of the human mitochondrial ribosome
            identifies SMIM4 and TMEM223 as respiratory chain assembly factors
  JOURNAL   Elife 10, e68213 (2021)
   PUBMED   34969438
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1702)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 1702)
  AUTHORS   Zhang,S., Reljic,B., Liang,C., Kerouanton,B., Francisco,J.C.,
            Peh,J.H., Mary,C., Jagannathan,N.S., Olexiouk,V., Tang,C.,
            Fidelito,G., Nama,S., Cheng,R.K., Wee,C.L., Wang,L.C., Duek
            Roggli,P., Sampath,P., Lane,L., Petretto,E., Sobota,R.M.,
            Jesuthasan,S., Tucker-Kellogg,L., Reversade,B., Menschaert,G.,
            Sun,L., Stroud,D.A. and Ho,L.
  TITLE     Mitochondrial peptide BRAWNIN is essential for vertebrate
            respiratory complex III assembly
  JOURNAL   Nat Commun 11 (1), 1312 (2020)
   PUBMED   32161263
  REMARK    GeneRIF: Study demonstrate that BRAWNIN, a 71 a.a. peptide encoded
            by C12orf73, is essential for respiratory chain complex III (CIII)
            assembly. In human cells, BRAWNIN is induced by the energy-sensing
            AMPK pathway, and its depletion impairs mitochondrial ATP
            production. Findings demonstrate that BRAWNIN is essential for
            vertebrate oxidative phosphorylation.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC078819.24.
            
            On Nov 22, 2018 this sequence version replaced NM_001135570.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.75078.1,
                                           SRR1660807.146614.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA2144333,
                                           SAMN03267764 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378090.9/ ENSP00000367330.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-147               AC078819.24        33641-33787         c
            148-265             AC078819.24        33203-33320         c
            266-1702            AC078819.24        26766-28202         c
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q23.3"
     gene            1..1702
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
                     /note="ubiquinol-cytochrome c reductase complex assembly
                     factor 6"
                     /db_xref="GeneID:728568"
                     /db_xref="HGNC:HGNC:34450"
                     /db_xref="MIM:618812"
     exon            1..147
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
                     /inference="alignment:Splign:2.1.0"
     exon            148..265
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..373
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
                     /note="uncharacterized protein C12orf73; protein BRAWNIN"
                     /codon_start=1
                     /product="ubiquinol-cytochrome c reductase complex
                     assembly factor 6"
                     /protein_id="NP_001129042.1"
                     /db_xref="CCDS:CCDS44964.1"
                     /db_xref="GeneID:728568"
                     /db_xref="HGNC:HGNC:34450"
                     /db_xref="MIM:618812"
                     /translation="MPAGVPMSTYLKMFAASLLAMCAGAEVVHRYYRPDLTIPEIPPK
                     RGELKTELLGLKERKHKPQVSQQEELK"
     exon            266..1702
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
                     /inference="alignment:Splign:2.1.0"
     regulatory      611..616
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
                     /note="hexamer: AATATA"
     polyA_site      646
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
     regulatory      1132..1137
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
                     /note="hexamer: AGTAAA"
     polyA_site      1156
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
                     /note="major polyA site"
     regulatory      1674..1679
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
                     /note="hexamer: ATTAAA"
     polyA_site      1702
                     /gene="UQCC6"
                     /gene_synonym="BR; BRAWNIN; C12orf73"
ORIGIN      
        1 gtattttggg tccggcctgc tcgccgtccg ctccgtccgc ccttagacct gttgcccagc
       61 atccctgcag ttcgcggtac agtctctagt agagcgcgtg tatagaggca gagaggagtg
      121 aagtccacag ttcctctcct ccaagagcct gccgaccatg cccgcgggcg tgcccatgtc
      181 cacctacctg aaaatgttcg cagccagtct cctggccatg tgcgcagggg cagaagtggt
      241 gcacaggtac taccgaccgg acctgacaat acctgaaatt ccaccaaagc gtggagaact
      301 caaaacggag cttttgggac tgaaagaaag aaaacacaaa cctcaagttt ctcaacagga
      361 ggaacttaaa taactatgcc aagaattctg tgaataatat aagtcttaaa tatgtatttc
      421 ttaatttatt gcatcaaact acttgtcctt aagcacttag tctaatgcta actgcaagag
      481 gaggtgctca gtggatgttt agccgatacg ttgaaattta attacggttt gattgatatt
      541 tcttgaaaac tgccaaagca catatcatca aaccatttca tgaatatggt ttggaagatg
      601 tttagtcttg aatataacgc gaaatagaat atttgtaagt ctactatatg ggttgtcttt
      661 atttcatata aattaagaaa ttatttaaaa ctatgaacta gtttcattaa ttgaatttgc
      721 taagtggagt tatttctcat tgctaaactg ttttttttca aattctagat ctctgctaac
      781 tttagtaaat gaacactaaa gtggtgagct ttgataatgc attttgtgga tacaaaatca
      841 tcgcctcatc actttctaga atgaaaggct gggggcattg ctcacctttt gcgtgaagaa
      901 aagacctgtc ccttagagcc agtttttgct taaggggagg acagaccgtc gtcacccagt
      961 ggaccacgtg aatgaatgga tgcagtgcat gctttagaag caccgcatac ctccttttac
     1021 aggtacagag taagtggtct ggctactgag ggaaagagtc atgatttgaa tccgagtctt
     1081 ctgtaagaca atgacttcat gaaggtgaaa aatcagtttt atggttatta aagtaaaaat
     1141 ttaccttggg taaaaatgcc atctttattt cattaagtac tggttctaag tgacttccaa
     1201 taagatacat tttcttcctg ttttgaagtt gatccccatt gtgaaaaata tacattgtag
     1261 tggtcatgga aatgcgccat ccatactccc taaggcaggc ttgttgccct agtatcaagc
     1321 atgccagcag ctttcaatgg tcagcttcta gaaggattgc ctcggctatg gtggccttgc
     1381 tcaaagccat gcctcctgaa ggggcccaaa tccagtgact gttggaggtg agaatagaaa
     1441 ggcctggcca cttcagcacg tgagtggtgg gcaactctgc tgggcccacc acaactccct
     1501 atggggttta gggacactgt ctttggatct gcattgcaac tcaacttata cctctgctca
     1561 gtcctgtttc ctttctcagg tgttgatcga agggtgtgcc ttcatagacc ccctgctaaa
     1621 ctgcacctca gtgtttcttg gagaaccaaa tctgtaacat atatgcatta aaaattaaag
     1681 gcagtactat attatcccct aa
//
