LOCUS       NM_145052               2474 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens uracil phosphoribosyltransferase homolog (UPRT),
            transcript variant 1, mRNA.
ACCESSION   NM_145052
VERSION     NM_145052.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2474)
  AUTHORS   Ghosh,A.C., Shimell,M., Leof,E.R., Haley,M.J. and O'Connor,M.B.
  TITLE     UPRT, a suicide-gene therapy candidate in higher eukaryotes, is
            required for Drosophila larval growth and normal adult lifespan
  JOURNAL   Sci Rep 5, 13176 (2015)
   PUBMED   26271729
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2474)
  AUTHORS   Zhao,F.J., Zhang,S., Yu,Z.M., Xia,S.J. and Li,H.
  TITLE     Specific targeting of prostate cancer cells in vitro by the suicide
            gene/prodrug system, uracil
            phosphoribosyltransferase/5-fluorouracil, under the control of
            prostate-specific membrane antigen promoter/enhancer
  JOURNAL   Prostate Cancer Prostatic Dis 12 (2), 166-171 (2009)
   PUBMED   18626508
  REMARK    GeneRIF: the efficacy of 5-FU-based chemotherapy in prostate
            cancers can be significantly improved by targeted expression of the
            suicide gene UPRT under the control of prostate-specific membrane
            antigen promoter/enhancer
REFERENCE   5  (bases 1 to 2474)
  AUTHORS   Li,J., Huang,S., Chen,J., Yang,Z., Fei,X., Zheng,M., Ji,C., Xie,Y.
            and Mao,Y.
  TITLE     Identification and characterization of human uracil
            phosphoribosyltransferase (UPRTase)
  JOURNAL   J Hum Genet 52 (5), 415-422 (2007)
   PUBMED   17384901
  REMARK    GeneRIF: isolation, expression, purification, and characterization
            of human uracil phosphoribosyltransferase
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA512889.1, BC015116.1,
            AL590234.7, AL137013.4 and AA974609.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_145052.3.
            
            Summary: This gene encodes uracil phosphoribosyltransferase, which
            catalyzes the conversion of uracil and
            5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (UMP).
            This reaction is an important part of nucleotide metabolism,
            specifically the pyrimidine salvage pathway. The enzyme localizes
            to the nucleus and cytoplasm. The protein is a potential target for
            rational design of drugs to treat parasitic infections and cancer.
            [provided by RefSeq, Nov 2009].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.66979.1,
                                           SRR1803613.111607.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000373383.9/ ENSP00000362481.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                DA512889.1         27-67
            42-546              BC015116.1         3-507
            547-555             AL590234.7         24464-24472
            556-1116            BC015116.1         517-1077
            1117-2038           AL137013.4         125309-126230       c
            2039-2186           BC015116.1         2000-2147
            2187-2474           AA974609.1         13-300              c
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq13.3"
     gene            1..2474
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /note="uracil phosphoribosyltransferase homolog"
                     /db_xref="GeneID:139596"
                     /db_xref="HGNC:HGNC:28334"
                     /db_xref="MIM:300656"
     exon            1..556
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..1100
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /EC_number="2.4.2.9"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UPRTase; UMP pyrophosphorylase; RP11-311P8.3; uracil
                     phosphoribosyltransferase (FUR1) homolog"
                     /codon_start=1
                     /product="uracil phosphoribosyltransferase homolog isoform
                     1"
                     /protein_id="NP_659489.1"
                     /db_xref="CCDS:CCDS14429.1"
                     /db_xref="GeneID:139596"
                     /db_xref="HGNC:HGNC:28334"
                     /db_xref="MIM:300656"
                     /translation="MATELQCPDSMPCHNQQVNSASTPSPEQLRPGDLILDHAGGNRA
                     SRAKVILLTGYAHSSLPAELDSGACGGSSLNSEGNSGSGDSSSYDAPAGNSFLEDCEL
                     SRQIGAQLKLLPMNDQIRELQTIIRDKTASRGDFMFSADRLIRLVVEEGLNQLPYKEC
                     MVTTPTGYKYEGVKFEKGNCGVSIMRSGEAMEQGLRDCCRSIRIGKILIQSDEETQRA
                     KVYYAKFPPDIYRRKVLLMYPILSTGNTVIEAVKVLIEHGVQPSVIILLSLFSTPHGA
                     KSIIQEFPEITILTTEVHPVAPTHFGQKYFGTD"
     exon            557..599
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /inference="alignment:Splign:2.1.0"
     exon            600..669
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /inference="alignment:Splign:2.1.0"
     exon            670..732
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /inference="alignment:Splign:2.1.0"
     exon            733..894
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /inference="alignment:Splign:2.1.0"
     exon            895..993
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /inference="alignment:Splign:2.1.0"
     exon            994..2474
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2170..2175
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /note="hexamer: ATTAAA"
     polyA_site      2189
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /note="major polyA site"
     regulatory      2438..2443
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /note="hexamer: AATAAA"
     regulatory      2452..2457
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
                     /note="hexamer: AATAAA"
     polyA_site      2460
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
     polyA_site      2474
                     /gene="UPRT"
                     /gene_synonym="FUR1; UPP"
ORIGIN      
        1 aaagtgtgct aaaggaaacc aacagcggcc taggggtgaa aggacagcca gggttagatg
       61 ttctgaggag gcgggagcaa ccgagagagc acgtgagcat ctgtcctttc tacccgttcc
      121 tctttatctt tagtgttcag tagcagcggg gatagcccgg ggcccggtgt atggccacgg
      181 agttacagtg tccggactcc atgccctgtc acaaccagca agtaaactct gcctcaaccc
      241 caagtcccga gcagctgcga cctggcgatc tgatcctgga ccacgcaggg ggaaacagag
      301 cctccagggc caaggtgatt ctcctcacgg ggtacgccca ttctagcctg ccggccgagc
      361 tggactctgg ggcctgcggc ggctccagcc tcaactcaga gggcaacagt ggtagtggtg
      421 acagtagcag ctatgacgca ccagctggca actccttcct agaggactgc gaactctccc
      481 ggcagatcgg ggcgcagctt aagctgctgc ctatgaatga tcagatacgg gagctacaga
      541 ccatcatccg tgacaagaca gccagtagag gtgacttcat gttttctgcg gatcgtttga
      601 tcagacttgt tgtggaagag ggattgaatc agctgccata taaagaatgc atggtgacca
      661 ctccaacagg gtacaagtat gaaggagtga aatttgagaa gggaaattgt ggggtcagca
      721 taatgagaag cggtgaggca atggaacaag gtttacgaga ctgctgtcga tccatacgaa
      781 ttggaaagat cctgattcag agtgatgagg agacacaaag agccaaagta tattatgcca
      841 aattcccccc agacatttac cggagaaaag tccttctgat gtatccaatt ctcagcactg
      901 gaaatactgt aattgaagct gtaaaggttc ttatagaaca tggagttcaa cccagtgtta
      961 tcatcctact cagtctgttc tccactcctc atggtgccaa atcaatcatt caggagtttc
     1021 cagagatcac aattttaact actgaagttc atcctgttgc acctacacat tttggacaga
     1081 aatactttgg aacagactaa gttatttaag taaaataatt atcttatgta atattacaat
     1141 catgttttga ttttctattt gttttactga ttcacttgag ggtggcagag aaaaatgtgt
     1201 taaaatgctt tttagttttg gaagtgggta tatttgaggt tatatctcat ttagttattt
     1261 gtttactgtt ggcaccgaat tcaacaatga agtatatgca actcttacaa aacataaatt
     1321 ttaataatat tctaatgcaa attactgaac cctcagtgca ttaaaattat ttcctaattt
     1381 atgagcctct tagtttggag gttgcttgaa gccacaaata aaactcaaat gagagtcagt
     1441 tctttgtccc actgtagttt gcattttgtt tagtttggtt ttagctctac taaggtttca
     1501 catatgtggc tattgccaca ttcctctact gttttctttc tgatactgga catgctagta
     1561 acttggagag gagaataaaa gaatgtctcc tggaatttta cattcccatt aatgtcctca
     1621 ttcagttggc agacctagga tgtatggtta actctcaggc cattatgttt ttcatggctc
     1681 atttcccaaa ctgcattgtt ggtaggtgcc ttgctaagct gtaatgcagt gaagaagatg
     1741 gtggggcatt tttagtaatt ggaactgtaa cattaattta caagattgaa ccggggatgt
     1801 ctggatgctg acaagggaga gccaagtact gttaatgcca tcaatcctct aggaaggaat
     1861 tggtggtttt tctgaagtgt gggggaaagg gtggtttctt gctggatttc atggccagta
     1921 agtaaattta aatgcttcat ttttaatttg gatgtttctg ctagagacct ttgatttgga
     1981 aattagttat tttgcatgtt tgtcaactgt atatgtatga aaatagaaaa aaaccatgct
     2041 ttttgtaaaa acccctaaga tttgtatatg ttcatcttta ggcttctgaa agggagagaa
     2101 atcatattgg aaatcagtgt tggtgttctg aaatctgctt gtaaaactct catctcattt
     2161 tacattgata ttaaaacatt ttaacagtgt tggctagctg attacttgat taattatact
     2221 cccatggccc cataggaagc tgttgtgggc ctctgttcaa aagcacagaa aagagcagaa
     2281 ggaaatgtct tagaaagaat caaagctgat gaaatcagca aagattgggg gaaaggaagg
     2341 aagcctggtg tagattggat tatatttttc ctttggggac tagaatacta gaagaacttc
     2401 agtatatttt tccctttatt tttgaatact gtcatataat aaattgagat taataaatta
     2461 ctcatattac agtt
//
