LOCUS       NM_015542               5168 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens UPF2 regulator of nonsense mediated mRNA decay (UPF2),
            transcript variant 2, mRNA.
ACCESSION   NM_015542
VERSION     NM_015542.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Langer,L.M., Kurscheidt,K., Basquin,J., Bonneau,F., Iermak,I.,
            Basquin,C. and Conti,E.
  TITLE     UPF1 helicase orchestrates mutually exclusive interactions with the
            SMG6 endonuclease and UPF2
  JOURNAL   Nucleic Acids Res 52 (10), 6036-6048 (2024)
   PUBMED   38709891
  REMARK    GeneRIF: UPF1 helicase orchestrates mutually exclusive interactions
            with the SMG6 endonuclease and UPF2.
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Jiang,H., Zhang,Y., Hu,J., Wang,Z., Li,G. and Lu,Y.
  TITLE     An alternative spliced UPF2 transcript in pancreatic inflammatory
            myofibroblastic tumors
  JOURNAL   Biochem Biophys Res Commun 691, 149306 (2024)
   PUBMED   38056247
  REMARK    GeneRIF: An alternative spliced UPF2 transcript in pancreatic
            inflammatory myofibroblastic tumors.
REFERENCE   3  (bases 1 to 5168)
  AUTHORS   Xue,G., Maciej,V.D., Machado de Amorim,A., Pak,M., Jayachandran,U.
            and Chakrabarti,S.
  TITLE     Modulation of RNA-binding properties of the RNA helicase UPF1 by
            its activator UPF2
  JOURNAL   RNA 29 (2), 178-187 (2023)
   PUBMED   36456182
  REMARK    GeneRIF: Modulation of RNA-binding properties of the RNA helicase
            UPF1 by its activator UPF2.
REFERENCE   4  (bases 1 to 5168)
  AUTHORS   Bufton,J.C., Powers,K.T., Szeto,J.A., Toelzer,C., Berger,I. and
            Schaffitzel,C.
  TITLE     Structures of nonsense-mediated mRNA decay factors UPF3B and UPF3A
            in complex with UPF2 reveal molecular basis for competitive binding
            and for neurodevelopmental disorder-causing mutation
  JOURNAL   Nucleic Acids Res 50 (10), 5934-5947 (2022)
   PUBMED   35640974
  REMARK    GeneRIF: Structures of nonsense-mediated mRNA decay factors UPF3B
            and UPF3A in complex with UPF2 reveal molecular basis for
            competitive binding and for neurodevelopmental disorder-causing
            mutation.
REFERENCE   5  (bases 1 to 5168)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 5168)
  AUTHORS   Yamashita,A., Ohnishi,T., Kashima,I., Taya,Y. and Ohno,S.
  TITLE     Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein
            kinase, associates with components of the mRNA surveillance complex
            and is involved in the regulation of nonsense-mediated mRNA decay
  JOURNAL   Genes Dev 15 (17), 2215-2228 (2001)
   PUBMED   11544179
REFERENCE   7  (bases 1 to 5168)
  AUTHORS   Serin,G., Gersappe,A., Black,J.D., Aronoff,R. and Maquat,L.E.
  TITLE     Identification and characterization of human orthologues to
            Saccharomyces cerevisiae Upf2 protein and Upf3 protein
            (Caenorhabditis elegans SMG-4)
  JOURNAL   Mol Cell Biol 21 (1), 209-223 (2001)
   PUBMED   11113196
REFERENCE   8  (bases 1 to 5168)
  AUTHORS   Lykke-Andersen,J., Shu,M.D. and Steitz,J.A.
  TITLE     Human Upf proteins target an mRNA for nonsense-mediated decay when
            bound downstream of a termination codon
  JOURNAL   Cell 103 (7), 1121-1131 (2000)
   PUBMED   11163187
REFERENCE   9  (bases 1 to 5168)
  AUTHORS   Mendell,J.T., Medghalchi,S.M., Lake,R.G., Noensie,E.N. and
            Dietz,H.C.
  TITLE     Novel Upf2p orthologues suggest a functional link between
            translation initiation and nonsense surveillance complexes
  JOURNAL   Mol Cell Biol 20 (23), 8944-8957 (2000)
   PUBMED   11073994
REFERENCE   10 (bases 1 to 5168)
  AUTHORS   Wilson,K.F., Fortes,P., Singh,U.S., Ohno,M., Mattaj,I.W. and
            Cerione,R.A.
  TITLE     The nuclear cap-binding complex is a novel target of growth factor
            receptor-coupled signal transduction
  JOURNAL   J Biol Chem 274 (7), 4166-4173 (1999)
   PUBMED   9933612
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA188353.1, BC114964.1 and
            AF301013.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015542.3.
            
            Summary: This gene encodes a protein that is part of a
            post-splicing multiprotein complex involved in both mRNA nuclear
            export and mRNA surveillance. mRNA surveillance detects exported
            mRNAs with truncated open reading frames and initiates
            nonsense-mediated mRNA decay (NMD). When translation ends upstream
            from the last exon-exon junction, this triggers NMD to degrade
            mRNAs containing premature stop codons. This protein is located in
            the perinuclear area. It interacts with translation release factors
            and the proteins that are functional homologs of yeast Upf1p and
            Upf3p. Two splice variants have been found for this gene; both
            variants encode the same protein. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) contains a different 5' UTR
            than variant 1 and is the shorter transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF301013.1, SRR1660805.177487.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357604.10/ ENSP00000350221.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 DA188353.1         33-36
            5-4944              BC114964.1         1-4940
            4945-5168           AF301013.1         4969-5192
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p14"
     gene            1..5168
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /note="UPF2 regulator of nonsense mediated mRNA decay"
                     /db_xref="GeneID:26019"
                     /db_xref="HGNC:HGNC:17854"
                     /db_xref="MIM:605529"
     exon            1..55
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            56..438
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..3892
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /note="smg-3 homolog, nonsense mediated mRNA decay factor;
                     yeast Upf2p homolog; nonsense mRNA reducing factor 2;
                     up-frameshift suppressor 2 homolog; UPF2 regulator of
                     nonsense transcripts homolog; LEMD3/UPF2 fusion;
                     FRS2/UPF2/LEMD3 fusion"
                     /codon_start=1
                     /product="regulator of nonsense transcripts 2"
                     /protein_id="NP_056357.1"
                     /db_xref="CCDS:CCDS7086.1"
                     /db_xref="GeneID:26019"
                     /db_xref="HGNC:HGNC:17854"
                     /db_xref="MIM:605529"
                     /translation="MPAERKKPASMEEKDSLPNNKEKDCSERRTVSSKERPKDDIKLT
                     AKKEVSKAPEDKKKRLEDDKRKKEDKERKKKDEEKVKAEEESKKKEEEEKKKHQEEER
                     KKQEEQAKRQQEEEAAAQMKEKEESIQLHQEAWERHHLRKELRSKNQNAPDSRPEENF
                     FSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIVEAKLKISD
                     VNCAVHLCSLFHQRYADFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVG
                     IFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKF
                     NLSFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELS
                     EDRHKQYEEFAMSYQKLLANSQSLADLLDENMPDLPQDKPTPEEHGPGIDIFTPGKPG
                     EYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSCQNKESNKDDTKEAKESKE
                     NKEVSSPDDLELELENLEINDDTLELEGGDEAEDLTKKLLDEQEQEDEEASTGSHLKL
                     IVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYAR
                     LVATLHPCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKN
                     DTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHL
                     DARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYVRKLLYKDLSKVTTEKVLRQMRKL
                     PWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLG
                     MEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSLDPPE
                     HLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDI
                     DYMISDTLELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKDSKDSMTEGENLE
                     EDEEEEEGGAETEEQSGNESEVNEPEEEEGSDNDDDEGEEEEEENTDYLTDSNKENET
                     DEENTEVMIKGGGLKHVPCVEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHL
                     KSQLRKGPPLGGGEGEAESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQA
                     EQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNAD
                     LIFKTGGRRR"
     exon            439..1218
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1379
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1577
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1727
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1831
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1917
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2026
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2140
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2257
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..2443
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..2647
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2648..2923
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2924..3107
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3108..3247
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3248..3352
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3353..3451
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3452..3619
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3761
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3762..3882
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     exon            3883..5168
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5150..5155
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /note="hexamer: AATAAA"
     polyA_site      5168
                     /gene="UPF2"
                     /gene_synonym="HUPF2; RENT2; smg-3"
                     /note="major polyA site"
ORIGIN      
        1 gagcgctgga gttggtgctg ggaaacccgg ggctaatgtt gacaacaggc tcgagattgt
       61 cctgggtcac ataatgccag ctgagcgtaa aaagccagca agtatggaag aaaaagactc
      121 tttaccaaac aacaaggaaa aagactgcag tgaaaggcgg acagtgagca gcaaggagag
      181 gccaaaagac gatatcaagc tcactgccaa gaaggaggtc agcaaggccc ctgaagacaa
      241 gaagaagaga ctggaagatg ataagagaaa aaaggaagac aaggaacgca agaaaaaaga
      301 cgaagaaaag gtgaaggcag aggaagaatc aaagaaaaaa gaagaggaag aaaaaaagaa
      361 acatcaagag gaagagagaa agaagcaaga agagcaggcc aaacgtcagc aagaagaaga
      421 agcagctgct cagatgaaag aaaaagaaga atccattcag cttcatcagg aagcttggga
      481 acgacatcat ttaagaaagg aacttcgtag caaaaaccaa aatgctccgg acagccgacc
      541 agaggaaaac ttcttcagcc gcctcgactc aagtttgaag aaaaatactg cttttgtcaa
      601 gaaactaaaa actattacag aacaacagag agactccttg tcccatgatt ttaatggcct
      661 aaatttaagc aaatacattg cagaagctgt agcttccatc gtggaagcaa aactaaaaat
      721 ctctgatgtg aactgtgctg tgcacctctg ctctctcttt caccagcgtt atgctgactt
      781 tgccccatca cttcttcagg tctggaaaaa acattttgaa gcaaggaaag aggagaaaac
      841 acctaacatc accaagttaa gaactgattt gcgttttatt gcagaattga caatagttgg
      901 gattttcact gacaaggaag gtctttcctt aatctatgaa cagctaaaaa atattattaa
      961 tgctgatcgg gagtcccaca ctcatgtctc tgtagtgatt agtttctgtc gacattgtgg
     1021 agatgatatt gctggacttg taccaaggaa agtaaagagt gctgcagaga agtttaattt
     1081 gagttttcct cctagtgaga taattagtcc agagaaacaa cagcccttcc agaatctttt
     1141 aaaagagtac tttacgtctt tgaccaaaca cctgaaaagg gaccacaggg agctccagaa
     1201 tactgagaga caaaacaggc gcattctaca ttctaaaggg gagctcagtg aagatagaca
     1261 taaacagtat gaggaatttg ctatgtctta ccagaagctg ctggcaaatt ctcaatcctt
     1321 agcagacctt ttggatgaaa atatgccaga tcttcctcaa gacaaaccaa caccagaaga
     1381 acatgggcct ggaattgata tattcacacc tggtaaacct ggagaatatg acttggaagg
     1441 tggtatatgg gaagatgaag atgctcggaa tttttatgag aacctcattg atttgaaggc
     1501 ttttgtccca gccatcttgt ttaaagacaa tgaaaaaagt tgtcagaata aagagtccaa
     1561 caaagatgat accaaagagg caaaagaatc taaggagaat aaggaggtat caagtcccga
     1621 tgatttggaa cttgagttgg agaatctaga aattaatgat gacaccttag aattagaggg
     1681 tggagatgaa gctgaagatc ttacaaagaa acttcttgat gaacaagaac aagaagatga
     1741 ggaagccagc actggatctc atctcaagct catagtagat gctttcctac agcagttacc
     1801 caactgtgtc aaccgagatc tgatagacaa ggcagcaatg gatttttgca tgaacatgaa
     1861 cacaaaagca aacaggaaga agttggtacg ggcactcttc atagttccta gacaaaggtt
     1921 ggatttgcta ccattttatg caagattggt tgctacattg catccctgca tgtctgatgt
     1981 agcagaggat ctttgttcca tgctgagggg ggatttcaga tttcatgtac ggaaaaagga
     2041 ccagatcaat attgaaacaa agaataaaac tgttcgtttt ataggagaac taactaagtt
     2101 taagatgttc accaaaaatg acacactgca ttgtttaaag atgcttctgt cagacttctc
     2161 tcatcaccat attgaaatgg catgcaccct gctggagaca tgtggacggt ttcttttcag
     2221 atctccagaa tctcacctga ggaccagtgt acttttggag caaatgatga gaaagaagca
     2281 agcaatgcat cttgatgcga gatacgtcac aatggtagag aatgcatatt actactgcaa
     2341 cccacctcca gctgaaaaaa ccgtgaaaaa gaaacgtcct cctctccagg aatatgtccg
     2401 gaaacttttg tacaaggatc tctctaaggt taccaccgag aaggttttga gacagatgcg
     2461 aaagctgccc tggcaggacc aagaagtgaa agactatgtt atttgttgta tgataaacat
     2521 ctggaatgtg aaatataata gtattcattg tgtagccaac ctcttagcag gactagtgct
     2581 ctaccaagag gatgttggga tccacgttgt ggatggagtg ttagaagata ttcgattagg
     2641 aatggaggtt aatcaaccta aatttaatca gaggcgcatc agcagtgcca agttcttagg
     2701 agaactttac aattaccgaa tggtggaatc agctgttatt ttcagaactc tgtattcttt
     2761 tacctcattt ggtgttaatc ctgatggctc tccaagttcc ctggacccac ctgagcatct
     2821 tttcagaatt agactcgtat gcactattct ggacacatgt ggccagtact ttgacagagg
     2881 ttccagtaaa cgaaaacttg attgtttcct tgtatatttt cagcgttatg tttggtggaa
     2941 gaaaagtttg gaggtttgga caaaagacca tccatttcct attgatatag attacatgat
     3001 cagtgataca ctagaactgc taagaccaaa gatcaaactc tgtaattctc tggaagaatc
     3061 catcaggcag gtacaagact tggaacgaga attcttaata aaactaggcc tagtaaatga
     3121 caaagactca aaagattcta tgacagaagg agaaaatctt gaagaggatg aagaagaaga
     3181 agaaggtggg gctgaaacag aagaacaatc tggaaatgaa agtgaagtaa atgagccaga
     3241 agaagaggag ggttctgata atgatgatga tgagggagaa gaagaggagg aagagaatac
     3301 agattacctt acagattcca ataaggaaaa tgaaaccgat gaagagaata ctgaggtaat
     3361 gattaaaggc ggtggactta agcatgtacc ttgtgtagaa gatgaggact tcattcaagc
     3421 tctggataaa atgatgctag aaaatctaca gcaacgaagt ggtgaatctg ttaaagtgca
     3481 ccaactagat gttgccattc ctttgcatct caaaagccag ctgaggaaag ggcccccact
     3541 gggaggtggg gaaggagagg ctgagtctgc agacacaatg ccgtttgtca tgttaacaag
     3601 aaaaggcaat aaacagcagt ttaagatcct taatgtaccc atgtcctctc aacttgctgc
     3661 aaatcactgg aaccagcaac aggcagaaca agaagagagg atgagaatga aaaagctcac
     3721 actagatatc aatgaacggc aagaacaaga agattatcaa gaaatgttgc agtctcttgc
     3781 acagcgccca gctccagcaa acaccaatcg tgagaggcgg cctcgctacc aacatccgaa
     3841 gggagcacct aatgcagatc taatctttaa gactggtggg aggagacgtt gatccagcag
     3901 cacgtgtcat ttcattaggt cctgtatctg atgttgtggt tagtggagtc ctccagcaat
     3961 tgaatgagag cagtggacac atctcagcag gtcggtctag agagttgcga atctaaacct
     4021 gggacaggct ggggccagga ggcagaaaca ccagcctctg ccaacaccgg aacaagccga
     4081 cgcttccaga caaggcggaa aaggcctttt gtaatggaaa tctcgcgagg gttaatcttc
     4141 tcttgagaat ggcagtcaag aaatgagatg gttcacttga ctactgagca gttacaccaa
     4201 ggagagcgtg aaggagatga ttgagccaga gaagaaacgg gttgtgatgg taatggtgtg
     4261 ggggaaatga acttgagctt taaacttgat ttgagtttca gtgtctctga attgaacatc
     4321 ccacgttgga agaagataca tttgggggct ccaggactac agtagaaaag tatagagcaa
     4381 gcaggaaaat cttctagtaa aacttacatg caggacaaca aaatgatgaa agatatccaa
     4441 ataccagata atccaccagg aaggcttttg tttaggaatt tgtttcaaga ggaacaaggg
     4501 atgagggaga aaaatccgtt ttatccatca gagtcagtgc tataaaattg cctattaagg
     4561 taaaagaaaa atgtggagac tattttacta tacagagagc attaattcag atggcttaga
     4621 aaagtgatac cagcccaaga acagggatct aggtgagccc attgtaagta tcattgaaaa
     4681 caaaacatgc ccgtcaacat gtcacagaaa acgaacgaag gacaacaaga agtggatgag
     4741 aatattttgt tgaccttcat gggtttacag cctctgtctc taaacaaagt atggaaacaa
     4801 gtagagcttt tattttgctt ttgtttttgt tttgtttttt tttttgtttt cccccactaa
     4861 atagaaatga gggtccttag tctgtttctg acaatctgtt aatttcttag gacagctgtc
     4921 tttggtttgc tttccagcag gcgtagtata tttagtcgga gagcacatct gtatgcgaca
     4981 acttgattac atcttttttt ctagctattt tgcatttttt cttttaccat gtttcagttt
     5041 ctgcatgtag atttaaataa aaaacaaaac ttgtaaagtt gtaacatttc acatggaaat
     5101 gctgcccaat cttcaccagc ttcagaaatc tgacctttgc cgatgctgca ataaagtgtt
     5161 gtaattta
//
