LOCUS       NM_002911               5321 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens UPF1 RNA helicase and ATPase (UPF1), transcript
            variant 2, mRNA.
ACCESSION   NM_002911
VERSION     NM_002911.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Langer,L.M., Kurscheidt,K., Basquin,J., Bonneau,F., Iermak,I.,
            Basquin,C. and Conti,E.
  TITLE     UPF1 helicase orchestrates mutually exclusive interactions with the
            SMG6 endonuclease and UPF2
  JOURNAL   Nucleic Acids Res 52 (10), 6036-6048 (2024)
   PUBMED   38709891
  REMARK    GeneRIF: UPF1 helicase orchestrates mutually exclusive interactions
            with the SMG6 endonuclease and UPF2.
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Chapman,J.H., Youle,A.M., Grimme,A.L., Neuman,K.C. and Hogg,J.R.
  TITLE     UPF1 ATPase autoinhibition and activation modulate RNA binding
            kinetics and NMD efficiency
  JOURNAL   Nucleic Acids Res 52 (9), 5376-5391 (2024)
   PUBMED   38412299
  REMARK    GeneRIF: UPF1 ATPase autoinhibition and activation modulate RNA
            binding kinetics and NMD efficiency.
REFERENCE   3  (bases 1 to 5321)
  AUTHORS   Musaev,D., Abdelmessih,M., Vejnar,C.E., Yartseva,V., Weiss,L.A.,
            Strayer,E.C., Takacs,C.M. and Giraldez,A.J.
  TITLE     UPF1 regulates mRNA stability by sensing poorly translated coding
            sequences
  JOURNAL   Cell Rep 43 (4), 114074 (2024)
   PUBMED   38625794
  REMARK    GeneRIF: UPF1 regulates mRNA stability by sensing poorly translated
            coding sequences.
REFERENCE   4  (bases 1 to 5321)
  AUTHORS   Machida,R., Ogawa,T., Min Soe,K. and Moriyama,K.
  TITLE     Nonsense-mediated mRNA decay affects hyperactive root formation in
            oculo-facio-cardio-dental syndrome via up-frameshift protein 1
  JOURNAL   J Oral Biosci 66 (1), 225-231 (2024)
   PUBMED   38244688
  REMARK    GeneRIF: Nonsense-mediated mRNA decay affects hyperactive root
            formation in oculo-facio-cardio-dental syndrome via up-frameshift
            protein 1.
            Erratum:[J Oral Biosci. 2024 Jun;66(2):483. doi:
            10.1016/j.job.2024.03.003.. PMID: 38871435]
REFERENCE   5  (bases 1 to 5321)
  AUTHORS   Jung,S., Ko,S.H., Ahn,N., Lee,J., Park,C.H. and Hwang,J.
  TITLE     Role of UPF1-LIN28A interaction during early differentiation of
            pluripotent stem cells
  JOURNAL   Nat Commun 15 (1), 158 (2024)
   PUBMED   38167913
  REMARK    GeneRIF: Role of UPF1-LIN28A interaction during early
            differentiation of pluripotent stem cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5321)
  AUTHORS   Page,M.F., Carr,B., Anders,K.R., Grimson,A. and Anderson,P.
  TITLE     SMG-2 is a phosphorylated protein required for mRNA surveillance in
            Caenorhabditis elegans and related to Upf1p of yeast
  JOURNAL   Mol Cell Biol 19 (9), 5943-5951 (1999)
   PUBMED   10454541
REFERENCE   7  (bases 1 to 5321)
  AUTHORS   Czaplinski,K., Ruiz-Echevarria,M.J., Paushkin,S.V., Han,X.,
            Weng,Y., Perlick,H.A., Dietz,H.C., Ter-Avanesyan,M.D. and
            Peltz,S.W.
  TITLE     The surveillance complex interacts with the translation release
            factors to enhance termination and degrade aberrant mRNAs
  JOURNAL   Genes Dev 12 (11), 1665-1677 (1998)
   PUBMED   9620853
REFERENCE   8  (bases 1 to 5321)
  AUTHORS   Applequist,S.E., Selg,M., Raman,C. and Jack,H.M.
  TITLE     Cloning and characterization of HUPF1, a human homolog of the
            Saccharomyces cerevisiae nonsense mRNA-reducing UPF1 protein
  JOURNAL   Nucleic Acids Res 25 (4), 814-821 (1997)
   PUBMED   9064659
REFERENCE   9  (bases 1 to 5321)
  AUTHORS   Perlick,H.A., Medghalchi,S.M., Spencer,F.A., Kendzior,R.J. Jr. and
            Dietz,H.C.
  TITLE     Mammalian orthologues of a yeast regulator of nonsense transcript
            stability
  JOURNAL   Proc Natl Acad Sci U S A 93 (20), 10928-10932 (1996)
   PUBMED   8855285
REFERENCE   10 (bases 1 to 5321)
  AUTHORS   Horikoshi,M., Sekimizu,K. and Natori,S.
  TITLE     Analysis of the stimulatory factor of RNA polymerase II in the
            initiation and elongation complex
  JOURNAL   J Biol Chem 259 (1), 608-611 (1984)
   PUBMED   6200476
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB960545.1, BC039817.1 and
            BQ950083.1.
            
            On Jun 3, 2019 this sequence version replaced NM_002911.3.
            
            Summary: This gene encodes a protein that is part of a
            post-splicing multiprotein complex involved in both mRNA nuclear
            export and mRNA surveillance. mRNA surveillance detects exported
            mRNAs with truncated open reading frames and initiates
            nonsense-mediated mRNA decay (NMD). When translation ends upstream
            from the last exon-exon junction, this triggers NMD to degrade
            mRNAs containing premature stop codons. This protein is located
            only in the cytoplasm. When translation ends, it interacts with the
            protein that is a functional homolog of yeast Upf2p to trigger mRNA
            decapping. Use of multiple polyadenylation sites has been noted for
            this gene. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. It
            encodes isoform 2, which is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC039817.1, AF074016.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262803.10/ ENSP00000262803.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-535               CB960545.1         57-591
            536-4218            BC039817.1         557-4239
            4219-4928           BQ950083.1         9-718
            4929-5321           BC039817.1         4950-5342
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.11"
     gene            1..5321
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /note="UPF1 RNA helicase and ATPase"
                     /db_xref="GeneID:5976"
                     /db_xref="HGNC:HGNC:9962"
                     /db_xref="MIM:601430"
     exon            1..482
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     CDS             252..3608
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /EC_number="3.6.4.13"
                     /EC_number="3.6.4.12"
                     /note="isoform 2 is encoded by transcript variant 2; delta
                     helicase; smg-2 homolog, nonsense mediated mRNA decay
                     factor; regulator of nonsense transcripts 1; nonsense mRNA
                     reducing factor 1; yeast Upf1p homolog; ATP-dependent
                     helicase RENT1; up-frameshift suppressor 1 homolog;
                     up-frameshift mutation 1 homolog; UPF1 regulator of
                     nonsense transcripts homolog"
                     /codon_start=1
                     /product="regulator of nonsense transcripts 1 isoform 2"
                     /protein_id="NP_002902.2"
                     /db_xref="CCDS:CCDS12386.1"
                     /db_xref="GeneID:5976"
                     /db_xref="HGNC:HGNC:9962"
                     /db_xref="MIM:601430"
                     /translation="MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQT
                     QTPPGGPGGPGGGGAGGPGGAGAGAAAGQLDAQVGPEGILQNGAVDDSVAKTSQLLAE
                     LNFEEDEEDTYYTKDLPIHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHL
                     VRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSS
                     LKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSAT
                     LEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWD
                     LGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIA
                     IELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEV
                     EDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYH
                     LARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIR
                     NMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPR
                     LAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQ
                     SLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWP
                     QPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEG
                     QRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDP
                     RRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFS
                     KPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSSMYFQTHDQIGMISAG
                     PSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGP
                     SQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFK
                     SQIDVALSQDSTYQGERAYQHGGVTGLSQY"
     exon            483..622
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            623..712
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            713..880
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1061
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1223
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            1224..1308
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1407
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1516
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1676
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            1677..1795
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1960
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2075
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..2219
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2220..2433
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2434..2551
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2708
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2709..2851
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2852..3026
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            3027..3108
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            3109..3270
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            3271..3488
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            3489..3611
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     exon            3612..5321
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5302..5307
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /note="hexamer: AATAAA"
     polyA_site      5321
                     /gene="UPF1"
                     /gene_synonym="HUPF1; NORF1; pNORF1; RENT1; smg-2; UTF"
                     /note="major polyA site"
ORIGIN      
        1 agttcctgct ctaggctgcg agcggctggc ggcttcgagg ggagctgagg cgcggagggg
       61 ctcggcggca gcggcggcgg ctcggcactg ttacctctcg gtccggctgg cgccggggcg
      121 cgcggtttgg tcctttccgg gcgcgcgggg gcgacagcgg cagcgacccg aggcctgcgg
      181 cctaggcctc agcgcggcgg cgggctcgag tgcagcgcgg aaccggcccg agggccctac
      241 ccggaggcac catgagcgtg gaggcgtacg ggcccagctc gcagactctc actttcctgg
      301 acacggagga ggccgagctg cttggcgccg acacacaggg ctccgagttc gagttcaccg
      361 actttactct tcctagccag acgcagacgc cccccggcgg ccccggcggc ccgggcggtg
      421 gcggcgcggg aggcccgggc ggcgcgggcg cgggcgctgc ggcgggacag ctcgacgcgc
      481 aggttgggcc cgaaggcatc ctgcagaacg gggctgtgga cgacagtgta gccaagacca
      541 gccagttgtt ggctgagttg aacttcgagg aagatgaaga agacacctat tacacgaagg
      601 acctccccat acacgcctgc agttactgtg gaatacacga tcctgcctgc gtggtttact
      661 gtaataccag caagaagtgg ttctgcaacg gacgtggaaa tacttctggc agccacattg
      721 taaatcacct tgtgagggca aaatgcaaag aggtgaccct gcacaaggac gggcccctgg
      781 gggagacagt cctggagtgc tacaactgcg gctgtcgcaa cgtcttcctc ctcggcttca
      841 tcccggccaa agctgactca gtggtggtgc tgctgtgcag gcagccctgt gccagccaga
      901 gcagcctcaa ggacatcaac tgggacagct cgcagtggca gccgctgatc caggaccgct
      961 gcttcctgtc ctggctggtc aagatcccct ccgagcagga gcagctgcgg gcacgccaga
     1021 tcacggcaca gcagatcaac aagctggagg agctgtggaa ggaaaaccct tctgccacgc
     1081 tggaggacct ggagaagccg ggggtggacg aggagccgca gcatgtcctc ctgcggtacg
     1141 aggacgccta ccagtaccag aacatattcg ggcccctggt caagctggag gccgactacg
     1201 acaagaagct gaaggagtcc cagactcaag ataacatcac tgtcaggtgg gacctgggcc
     1261 ttaacaagaa gagaatcgcc tacttcactt tgcccaagac tgactctgac atgcggctca
     1321 tgcaggggga tgagatatgc ctgcggtaca aaggggacct tgcgcccctg tggaaaggga
     1381 tcggccacgt catcaaggtc cctgataatt atggcgatga gatcgccatt gagctgcgga
     1441 gcagcgtggg tgcacctgtg gaggtgactc acaacttcca ggtggatttt gtgtggaagt
     1501 cgacctcctt tgacaggatg cagagcgcat tgaaaacgtt tgccgtggat gagacctcgg
     1561 tgtctggcta catctaccac aagctgttgg gccacgaggt ggaggacgta atcatcaagt
     1621 gccagctgcc caagcgcttc acggcgcagg gcctccccga cctcaaccac tcccaggttt
     1681 atgccgtgaa gactgtgctg caaagaccac tgagcctgat ccagggcccg ccaggcacgg
     1741 ggaagacggt gacgtcggcc accatcgtct accacctggc ccggcaaggc aacgggccgg
     1801 tgctggtgtg tgctccgagc aacatcgccg tggaccagct aacggagaag atccaccaga
     1861 cggggctaaa ggtcgtgcgc ctctgcgcca agagccgtga ggccatcgac tccccggtgt
     1921 cttttctggc cctgcacaac cagatcagga acatggacag catgcctgag ctgcagaagc
     1981 tgcagcagct gaaagacgag actggggagc tgtcgtctgc cgacgagaag cggtaccggg
     2041 ccttgaagcg caccgcagag agagagctgc tgatgaacgc agatgtcatc tgctgcacat
     2101 gtgtgggcgc cggtgacccg aggctggcca agatgcagtt ccgctccatt ttaatcgacg
     2161 aaagcaccca ggccaccgag ccggagtgca tggttcccgt ggtcctcggg gccaagcagc
     2221 tgatccttgt aggcgaccac tgccagctgg gcccagtggt gatgtgcaag aaggcggcca
     2281 aggccgggct gtcacagtcg ctcttcgagc gcctggtggt gctgggcatc cggcccatcc
     2341 gcctgcaggt ccagtaccgg atgcaccctg cactcagcgc cttcccatcc aacatcttct
     2401 acgagggctc cctccagaat ggtgtcactg cagcggatcg tgtgaagaag ggatttgact
     2461 tccagtggcc ccaacccgat aaaccgatgt tcttctacgt gacccagggc caagaggaga
     2521 ttgccagctc gggcacctcc tacctgaaca ggaccgaggc tgcgaacgtg gagaagatca
     2581 ccacgaagtt gctgaaggca ggcgccaagc cggaccagat tggcatcatc acgccctacg
     2641 agggccagcg ctcctacctg gtgcagtaca tgcagttcag cggctccctg cacaccaagc
     2701 tctaccagga ggtggagatc gccagtgtgg acgcctttca gggacgcgag aaggacttca
     2761 tcatcctgtc ctgtgtgcgg gccaacgagc accaaggcat tggcttttta aatgacccca
     2821 ggcgtctgaa cgtggccctg accagagcaa ggtatggcgt catcattgtg ggcaacccga
     2881 aggcactatc aaagcagccg ctctggaacc acctgctgaa ctactataag gagcagaagg
     2941 tgctggtgga ggggccgctc aacaacctgc gtgagagcct catgcagttc agcaagccac
     3001 ggaagctggt caacactatc aacccgggag cccgcttcat gaccacagcc atgtatgatg
     3061 cccgggaggc catcatccca ggctccgtct atgatcggag cagccagggc cggccttcca
     3121 gcatgtactt ccagacccat gaccagattg gcatgatcag tgccggccct agccacgtgg
     3181 ctgccatgaa cattcccatc cccttcaacc tggtcatgcc acccatgcca ccgcctggct
     3241 attttggaca agccaacggg cctgctgcag ggcgaggcac cccgaaaggc aagactggtc
     3301 gtgggggacg ccagaagaac cgctttgggc ttcctggacc cagccagact aacctcccca
     3361 acagccaagc cagccaggat gtggcgtcac agcccttctc tcagggcgcc ctgacgcagg
     3421 gctacatctc catgagccag ccttcccaga tgagccagcc cggcctctcc cagccggagc
     3481 tgtcccagga cagttacctt ggtgacgagt ttaaatcaca aatcgacgtg gcgctctcac
     3541 aggactccac gtaccaggga gagcgggctt accagcatgg cggggtgacg gggctgtccc
     3601 agtattaaaa ggtggcggcg gaagagctaa gcaacgtggc ttagtccatc agcatcttat
     3661 tctgggtaat aaaaaataaa aataaacgga tacctgtttt ccactgctaa aactgaagca
     3721 ccactgtgtg agcaacagga agggagagcg cacgagggag aggagccgag gccgagcgcc
     3781 ccctgctggc ccgcggcggc gaggagcaga gggagcggag gaggggccgg cccgcgggag
     3841 ccgcggccac caggaggccc cgctccgtcc catcggggct gcggccaggg cggagggagg
     3901 aagaccctca tctcagagta gccctttcct ctgttctttt atttcttttt ctctttgatt
     3961 gaaaggggac tacgtcttag caggaaaaaa aacttcgcat ttctgtgccc gagcaggctc
     4021 cttgcaaaga cagcagcgtg cggggcagag ccccgggagg gcgcgtctgt ccacgcctac
     4081 cggacgcgcc gaggtcgcgc tgcctgtgtt ctccgagggc cttcatttaa agaaaataag
     4141 ggtgttttgg gtttttctct ttgttttttt caagattctt ttaaaggagt actgaagaat
     4201 actttcctaa gtttgtctgt aaaatcttag cggtggacct gggagatttg agaagcttcc
     4261 agaaacagtt taaacaagcc agcgctactg gagaagagga gcaacacctg tgccgcggcc
     4321 ggaggagttt tgttgttggt tttagcttcc agtggcttct ttctgcgggg catcaggctg
     4381 ctggggtagc cgcccgccga gcctggaagc tgctcgttct ccgctggact cagaagccaa
     4441 gctgcttccc gcctagactc ggcgcagggc cccgcaccgg tgaggaaggt gcttttggcc
     4501 ccattgcgag gggccttggc caggactggc cctgtggcca ggaggcgaga aggtggctgt
     4561 tcccggattg acggcttttt cccgggggcc tttggaagat ttggtggaag gacaagaggg
     4621 cctgtccctg tccccgtccc caggaggtac cgacagtccc tgtgctggtt agacacggag
     4681 cgctgcacac cgaaagccca aattgggagc tctgcctgcc ggcaactttg ctgatggggt
     4741 gattgctgct tctggggggt aaggaaacaa gttacagaaa ttaccgcgtt ctgtgtgaag
     4801 ggactgaggg tgtggtgtca ttggcagagg gtcattttag gagagctgcc ccagcccctc
     4861 gaacgcctgg cttggggtgt cattctgcct ggcggccagg cctccagctt cccctgcccc
     4921 gggcctgggg ctgtcactgg ccctgatccg aacacctcca gattccggct tctacatggg
     4981 acagacgggg acgcacaggc caccttcctt ctggcaggga ctcttattta ttcccattgc
     5041 tctagggctt tcggtttccc cttcttccgg taggccgcgt agaggcatgc accgggtagg
     5101 tttccgcggt gaccccgcgg cggcctgagg gacgctccct gccccatccc ggctgttggg
     5161 ctgggccgct ttgcctctgc ttcgccctgt gctgtgttct ccagctttgt agcagcagcc
     5221 ttgacaaacc caggcgcact gtaccaaggc aatgtaactt ttgattttcg gtcaatttaa
     5281 gttcttttgt caccaaatat taataaacag ttttgacttc a
//
