LOCUS       NM_000373               6652 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens uridine monophosphate synthetase (UMPS), transcript
            variant 1, mRNA.
ACCESSION   NM_000373
VERSION     NM_000373.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Kim-Holzapfel,D.M., Dey,R., Richardson,B.C., Arachchige,D.,
            Reddy,K., De Vitto,H., Bhandari,J. and French,J.B.
  TITLE     Human uridine 5'-monophosphate synthase stores metabolic potential
            in inactive biomolecular condensates
  JOURNAL   J Biol Chem 299 (3), 102949 (2023)
   PUBMED   36708921
  REMARK    GeneRIF: Human uridine 5'-monophosphate synthase stores metabolic
            potential in inactive biomolecular condensates.
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 6652)
  AUTHORS   Luo,L., Zhang,J., Tang,H., Zhai,D., Huang,D., Ling,L., Wang,X.,
            Liu,T., Zhang,Q., Zhang,Z., He,Z. and Zheng,G.
  TITLE     LncRNA SNORD3A specifically sensitizes breast cancer cells to 5-FU
            by sponging miR-185-5p to enhance UMPS expression
  JOURNAL   Cell Death Dis 11 (5), 329 (2020)
   PUBMED   32382150
  REMARK    GeneRIF: LncRNA SNORD3A specifically sensitizes breast cancer cells
            to 5-FU by sponging miR-185-5p to enhance UMPS expression.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6652)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 6652)
  AUTHORS   Kim,S.Y., Baek,J.Y., Oh,J.H., Park,S.C., Sohn,D.K., Kim,M.J.,
            Chang,H.J., Kong,S.Y. and Kim,D.Y.
  TITLE     A phase II study of preoperative chemoradiation with tegafur-uracil
            plus leucovorin for locally advanced rectal cancer with
            pharmacogenetic analysis
  JOURNAL   Radiat Oncol 12 (1), 62 (2017)
   PUBMED   28347333
  REMARK    GeneRIF: The UMPS 638 CC genotype might be a candidate biomarker
            predicting toxicity in patients receiving
            tegafur-uracil/leucovorin-based preoperative chemoradiation for
            locally advanced rectal cancer
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6652)
  AUTHORS   Qumsiyeh,M.B., Valentine,M.B. and Suttle,D.P.
  TITLE     Localization of the gene for uridine monophosphate synthase to
            human chromosome region 3q13 by in situ hybridization
  JOURNAL   Genomics 5 (1), 160-162 (1989)
   PUBMED   2767686
REFERENCE   7  (bases 1 to 6652)
  AUTHORS   Suchi,M., Harada,N., Tsuboi,T., Asai,K., Okajima,K., Wada,Y. and
            Takagi,Y.
  TITLE     Molecular cloning of human UMP synthase
  JOURNAL   Adv Exp Med Biol 253A, 511-518 (1989)
   PUBMED   2624233
REFERENCE   8  (bases 1 to 6652)
  AUTHORS   Suttle,D.P., Bugg,B.Y., Winkler,J.K. and Kanalas,J.J.
  TITLE     Molecular cloning and nucleotide sequence for the complete coding
            region of human UMP synthase
  JOURNAL   Proc Natl Acad Sci U S A 85 (6), 1754-1758 (1988)
   PUBMED   3279416
REFERENCE   9  (bases 1 to 6652)
  AUTHORS   Patterson,D., Jones,C., Morse,H., Rumsby,P., Miller,Y. and Davis,R.
  TITLE     Structural gene coding for multifunctional protein carrying orotate
            phosphoribosyltransferase and OMP decarboxylase activity is located
            on long arm of human chromosome 3
  JOURNAL   Somatic Cell Genet 9 (3), 359-374 (1983)
   PUBMED   6574608
REFERENCE   10 (bases 1 to 6652)
  AUTHORS   McClard,R.W., Black,M.J., Livingstone,L.R. and Jones,M.E.
  TITLE     Isolation and initial characterization of the single polypeptide
            that synthesizes uridine 5'-monophosphate from orotate in Ehrlich
            ascites carcinoma. Purification by tandem affinity chromatography
            of uridine-5'-monophosphate synthase
  JOURNAL   Biochemistry 19 (20), 4699-4706 (1980)
   PUBMED   6893554
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC007511.2 and AC022336.12.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000373.3.
            
            Summary: This gene encodes a uridine 5'-monophosphate synthase. The
            encoded protein is a bifunctional enzyme that catalyzes the final
            two steps of the de novo pyrimidine biosynthetic pathway. The first
            reaction is carried out by the N-terminal enzyme orotate
            phosphoribosyltransferase which converts orotic acid to
            orotidine-5'-monophosphate. The terminal reaction is carried out by
            the C-terminal enzyme OMP decarboxylase which converts
            orotidine-5'-monophosphate to uridine monophosphate. Defects in
            this gene are the cause of hereditary orotic aciduria. Alternate
            splicing results in multiple transcript variants. [provided by
            RefSeq, Mar 2010].
            
            Transcript Variant: This variant (1) encodes the functional
            protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU921886.1, SRR1660807.20671.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000232607.7/ ENSP00000232607.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1678              BC007511.2         4-1681
            1679-6652           AC022336.12        35360-40333
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.2"
     gene            1..6652
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /note="uridine monophosphate synthetase"
                     /db_xref="GeneID:7372"
                     /db_xref="HGNC:HGNC:12563"
                     /db_xref="MIM:613891"
     exon            1..176
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..1463
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /EC_number="2.4.2.10"
                     /EC_number="4.1.1.23"
                     /note="orotate phosphoribosyltransferase; OPRTase;
                     orotidine 5'-phosphate decarboxylase; UMP synthase;
                     OMPdecase"
                     /codon_start=1
                     /product="uridine 5'-monophosphate synthase"
                     /protein_id="NP_000364.1"
                     /db_xref="CCDS:CCDS3029.1"
                     /db_xref="GeneID:7372"
                     /db_xref="HGNC:HGNC:12563"
                     /db_xref="MIM:613891"
                     /translation="MAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIV
                     SRPRLLSQVADILFQTAQNAGISFDTVCGVPYTALPLATVICSTNQIPMLIRRKETKD
                     YGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQKEGLKVTDAIVLLDREQGGK
                     DKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRFIQENVFVAANHNGSPLSI
                     KEAPKELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPS
                     ICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIA
                     SWADLVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEE
                     HSEFVVGFISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVG
                     RGIISAADRLEAAEMYRKAAWEAYLSRLGV"
     exon            177..330
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /inference="alignment:Splign:2.1.0"
     exon            331..1002
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1178
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1293
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..6652
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1657..1662
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /note="hexamer: AATAAG"
     polyA_site      1673
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /note="major polyA site"
     regulatory      5651..5656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /note="hexamer: ATTAAA"
     polyA_site      5677
                     /gene="UMPS"
                     /gene_synonym="OPRT"
     regulatory      6630..6635
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /note="hexamer: AATAGA"
     polyA_site      6652
                     /gene="UMPS"
                     /gene_synonym="OPRT"
ORIGIN      
        1 caaacaggca gcgcgcgaca atggcggtcg ctcgtgcagc tttggggcca ttggtgacgg
       61 gtctgtacga cgtgcaggct ttcaagtttg gggacttcgt gctgaagagc gggctttcct
      121 cccccatcta catcgatctg cggggcatcg tgtctcgacc gcgtcttctg agtcaggttg
      181 cagatatttt attccaaact gcccaaaatg caggcatcag ttttgacacc gtgtgtggag
      241 tgccttatac agctttgcca ttggctacag ttatctgttc aaccaatcaa attccaatgc
      301 ttattagaag gaaagaaaca aaggattatg gaactaagcg tcttgtagaa ggaactatta
      361 atccaggaga aacctgttta atcattgaag atgttgtcac cagtggatct agtgttttgg
      421 aaactgttga ggttcttcag aaggagggct tgaaggtcac tgatgccata gtgctgttgg
      481 acagagagca gggaggcaag gacaagttgc aggcgcacgg gatccgcctc cactcagtgt
      541 gtacattgtc caaaatgctg gagattctcg agcagcagaa aaaagttgat gctgagacag
      601 ttgggagagt gaagaggttt attcaggaga atgtctttgt ggcagcgaat cataatggtt
      661 ctcccctttc tataaaggaa gcacccaaag aactcagctt cggtgcacgt gcagagctgc
      721 ccaggatcca cccagttgca tcgaagcttc tcaggcttat gcaaaagaag gagaccaatc
      781 tgtgtctatc tgctgatgtt tcactggcca gagagctgtt gcagctagca gatgctttag
      841 gacctagtat ctgcatgctg aagactcatg tagatatttt gaatgatttt actctggatg
      901 tgatgaagga gttgataact ctggcaaaat gccatgagtt cttgatattt gaagaccgga
      961 agtttgcaga tataggaaac acagtgaaaa agcagtatga aggaggtatc tttaaaatag
     1021 cttcctgggc agatctagta aatgctcacg tggtgccagg ctcaggagtt gtgaaaggcc
     1081 tgcaagaagt gggcctgcct ttgcatcggg ggtgcctcct tattgcggaa atgagctcca
     1141 ccggctccct ggccactggg gactacacta gagcagcggt tagaatggct gaggagcact
     1201 ctgaatttgt tgttggtttt atttctggct cccgagtaag catgaaacca gaatttcttc
     1261 acttgactcc aggagttcag ttggaagcag gaggagataa tcttggccaa cagtacaata
     1321 gcccacaaga agttattggc aaacgaggtt ccgatatcat cattgtaggt cgtggcataa
     1381 tctcagcagc tgatcgtctg gaagcagcag agatgtacag aaaagctgct tgggaagcgt
     1441 atttgagtag acttggtgtt tgagtgcttc agatacattt ttcagataca atgtgaagac
     1501 attgaagata tgtggtcctc ctgaaagtca ctggctggaa ataatccaat tattcctgct
     1561 tggattcttc cacagggcct gtgtaagaat gggttctgga gttctcatgg tctttaggaa
     1621 atattgagta atttgtaatc accgcattga tactataata agttcattct taagcttgct
     1681 ttttttgaga ctggtgtttg ttagacagcc acagtcctgt ctgggttagg gtcttccaca
     1741 tttgaggatc cttcctatct ctccatggga ctagactgct ttgttattct atttattttt
     1801 taattttttt cgagacagga tctcactctg ttgcccagga tggagtgcag tggtgagatc
     1861 acggctcatt gcagcctcga cctcccaggt gatcctccca cctcagcttc cagattagct
     1921 ggtgctatag gcatgcacca ccacgtccat ctaaatttct ttattatttg tagagatgag
     1981 gtcttgccat gttacccagg ctggtctcaa ctcctgggct caagcgatcc tcctgcctca
     2041 gtctctcaaa gtgctgggat tacaggtgtg agccactgtg cccagcctaa ttgcagtaag
     2101 acaaaaattc tagggcacca agaggctaaa gtcagcacag cttttcttgt gtcctgtatt
     2161 ctctgtctaa tgtgttgccc aaataatacc taattgttag ccattcccct ccatctctgg
     2221 cctaaaagtg atagtccagg tatccacatg ggctggttcc cagaactgcc attgctcact
     2281 ctccaaagag gggaaggtgg ggaaggggaa ggtgactata gctcagctcc tgagctagta
     2341 tctggctgtt atttcaacaa ccggagttgg ggtttgggct cattttttcc cctagccagc
     2401 aattatggac cagtagtaac acaagtgaca gcttcctgtg actgacttca caattaggag
     2461 gtctaagatt ccatttgggt atttgcttaa ggatcccaca taattgtccc aacggtcatt
     2521 agtagagggg aggtaagcct tcattaataa taaagagaaa gcccacattc aaggtggtgt
     2581 ttgagcaggg gcagggtgag ggctgtcccg gtgctcattg caccagcaca ctcacattcc
     2641 ttctcatttg gggcccacct gcaggaagtg gcacaggatc agccatttcc ccacccttgt
     2701 cagctgatgg cccactgttc tttaatgact cagaggaatg cctaggattt tttttttttt
     2761 ttgagacaga atctcactgt cgcccaggct ggagttcagt ggcacgatct cggctcactg
     2821 caacttctgc ctctctggtt caagcagttc tcctgcctca gcctcccgag tagctgggac
     2881 tacaagccta ggatttttaa ctcaggtttt tattatattc cctcctgaag tttttacttc
     2941 aagagcttct gctctaaagt ccaatttggg cttcatgtcc ccagtgctgc atctccaggg
     3001 aaatgctgtc tgtgggagag accaactctc aaggaagaag tggccacaga aggagcagga
     3061 agggagttgg ccctcagggc tactctgggg aagccaaaag tcatgaaggg gagaagaatt
     3121 ttctgacaaa aacttgcagg aatctcttag gtgtcttcag tgttggagtg atatgttgag
     3181 aggcctttgg agtgatgtgc tgaggtctca ggcgcccacc tccctggctg tcacttccat
     3241 gtgtcagtgg ttctcccact ttagcaggta tcagagtcac ctggagtctt gtcaaaacag
     3301 gtaccagccc cacccgcagc gtttctgact ctgggtagct ctgggatggg gcttgagaat
     3361 ttgcgtttcc aaaaaggtcc caggtgatgc tgcggttgcc tgcgcaggga ctggactttg
     3421 agaaccactt cactggttat tcacatttct gcctctgcag tgagacagcc ttgaggtctg
     3481 cctcctgcta agagtcacat gctcctgtcc tttagaaatg tgggctcctg ccatctccag
     3541 gacgcaggca ctgttcctgt tgatgaaccc tatttcacag gacccctgct aaggtgattt
     3601 gaggggaaat gagaggaggc tcaaataatc acccagcccc tgccacttac tgaaagtgta
     3661 ggtccttgtg ccccacacca tcagagtttc tgcgttagca gatttgtggt ttgcccagca
     3721 gcctgggcgt gtgcatttct aatgggtgcc tcaagtgatc tgtttctgat ttgtatttct
     3781 attgtgaaga gtcagcccag tactgcaggc ctcttaccta agcagaatcc cagtctggca
     3841 tcaaagcttt agaggacaag ttgattcagg cagagaagaa cttgggctat acaagcgctg
     3901 ttcttcagca ttgaagtatt ttggaggcat tagatagttt aaccctttct cagtcaagga
     3961 atatttacag aacatgatct ctgggcattg taactcctgg tcttagtggg gaatataggg
     4021 accccatgtc tccatggggt gcacagaatg tctgtgagac tgatggagtg gagaacgcca
     4081 tcccccagcc tctccagcta ctcgaggcat tctgtagaac ataagcccat agattgtgtg
     4141 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgcatg cgcgcgcgtg cgcactggag
     4201 gaacctaaga aactatttgg tgcacttcct cttattttag agctcccaaa gtgtagctcc
     4261 agaatcgtaa agggatatgc tcagtctcac agccagcctg tggatctcag tcccaacact
     4321 cacccttgtg ctactgagtc agctctaaga aaatctgcca aaagtaggcc gagggctggt
     4381 tttttgtttt gttttgtttg tttgatacag ggtcttcact ctgttgccca ggctggagta
     4441 tatcatggct cactgcaacc ttgacttggg ctcaagcgat ccgctcaagt agctggaact
     4501 actctcaagt agctctcaag agcctctcga gtggctggaa ctacaggcgt gcaccaccac
     4561 agctggttaa tttttaaaat tttttgtaga gacggtggag gaggttctca ctgtgactca
     4621 gtgtgtgccc gacagcagag cccacaccac tccagttgca gtggttgcca tctgggtcat
     4681 cagacctggc tgtcaggggt gcagccacag gagagccaac agcagagggt gctggccgct
     4741 gagctagctg ctaatgctgg cctgggtgca gttctcatcc aaagtacccg gtgggtggga
     4801 gtcactcagt accagttccg agcctgaacc caaactctcg tgtttctgct cacccctctc
     4861 tggcttctgc caccacatgg gaagaatatg ccctggttag cccatggctt ctgaagagca
     4921 agagaaagta gagcagagcc tactccagcc tcccccgtcc aatgtatgaa agccccagct
     4981 gatctgtaag cctgggagcg tgataaatgc ttagtagtgc atgccatgga gttccagggt
     5041 ggtttattac acggcaatat ctagctaaat acatttaact tgctgcagct ctctggatcc
     5101 agcctggtta ccaggaagac aaaaactggg ctccaccagg aaccagtctt ctgccttccc
     5161 aaccatcacc tctggctgca tcagcgatct ctcccagcga aatagctgct tggtcttgtg
     5221 tgaatcctgt actttaacac agtggaccaa gtgtcagtca ttgaaaatga ccatgagtaa
     5281 ccctgtggac tctctgcagc ttggttcctt tgccccttaa caggtgggta tgaatcgtgt
     5341 cttcagtgcc agggctgaat gagaaagggc attccttttt gaaggaatct gatactaaac
     5401 acaaagcatg agaaaaatca ggacttgttg gagttatatt tttaaaatat atattttaac
     5461 agttatatat attagatata atatataata gtatatataa ataatactat attgcccagg
     5521 ctggtctcga actccttagc tcaagtgatc ctcctgcctt ggcttcccaa agtgctagga
     5581 ttacaggtgt gagccactgc tcccggcctg ttggagttct ttacatttat tttataatca
     5641 atgctgtttt attaaatgcg gattttattt tggattacag gatgtagaat gccatatttt
     5701 tcttagatca tagggccttt cacatttgta atttggcctt gtatgagtta ccctgcaatc
     5761 cctttgtttt ccccataacc cttccaaagg aaggccgcaa tagaaataca aagagaaaca
     5821 aaataattag aatatttttt aacttctaaa gttcaaggtt ttggcataag tctggtttag
     5881 aagcacattt gcctagccct ttccttccca ccaaggggga aagtcttcct ctagacaaga
     5941 ggcagagggc tcctcagagt cagatcctgg tgtgggctct cacgtgctgc tgctgaatcc
     6001 cagggaagga gggaggaagg gcagttgaca cccaaaataa gggtggggaa ctgtcagcag
     6061 aggaggtctg tgtcatgttt ttcagcgctg gggttggggg gagcccagga gagcaggaag
     6121 atccagagat ccctcgcccc agctcggcca tgtgtgtctg ggacagagcc tgaggtggcc
     6181 tgagcttcct gtggctccag agtaacatta tagagaagct gaattctcct gtttttctga
     6241 aaagggcatg ggagttagct gagaagcaga cctggtgggc ctgagagtct caatcgtcag
     6301 gtaaggacag tcagtgggaa gtggacgggc cgcacaacca aggttctcat gaggacaacc
     6361 atgtcttcgg gggtgccctt gtgcacagac agctccatag tcctgcctcc aatgtcccaa
     6421 cactgcattg tctccctgca cttagcagcc ctgcagggtg agacttgggg aggatcctga
     6481 aatgattgta tttaacaaga catgctgtcc ttgtttacct ggaacctagc aatgttgttt
     6541 tctgccacaa cttgaataga tacttgaagc agagatgatg ttgagttaaa aaaaatatat
     6601 acataaaaat atgggttctt ttcaacctga atagatggcc taaaaattca aa
//
