LOCUS       NM_003565               5322 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1),
            mRNA.
ACCESSION   NM_003565 XM_942125
VERSION     NM_003565.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Zhang,Z., Liu,X., Chu,C., Zhang,Y., Li,W., Yu,X., Han,Q., Sun,H.,
            Zhang,Y., Zhu,X., Chen,L., Wei,R., Fan,N., Zhou,M. and Li,X.
  TITLE     MIR937 amplification potentiates ovarian cancer progression by
            attenuating FBXO16 inhibition on ULK1-mediated autophagy
  JOURNAL   Cell Death Dis 15 (10), 735 (2024)
   PUBMED   39384743
  REMARK    GeneRIF: MIR937 amplification potentiates ovarian cancer
            progression by attenuating FBXO16 inhibition on ULK1-mediated
            autophagy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Sankar,D.S., Kaeser-Pebernard,S., Vionnet,C., Favre,S., de Oliveira
            Marchioro,L., Pillet,B., Zhou,J., Stumpe,M., Kovacs,W.J.,
            Kressler,D., Antonioli,M., Fimia,G.M. and Dengjel,J.
  TITLE     The ULK1 effector BAG2 regulates autophagy initiation by modulating
            AMBRA1 localization
  JOURNAL   Cell Rep 43 (9), 114689 (2024)
   PUBMED   39207901
  REMARK    GeneRIF: The ULK1 effector BAG2 regulates autophagy initiation by
            modulating AMBRA1 localization.
REFERENCE   3  (bases 1 to 5322)
  AUTHORS   Yong,M., Zeng,Y., Yao,Y., Yang,M., Tang,F., Zhu,H. and Hu,J.
  TITLE     CircFAM188A Regulates Autophagy via miR-670-3p and ULK1 in
            Epithelial Ovarian Carcinoma
  JOURNAL   Cancer Rep (Hoboken) 7 (9), e2128 (2024)
   PUBMED   39229655
  REMARK    GeneRIF: CircFAM188A Regulates Autophagy via miR-670-3p and ULK1 in
            Epithelial Ovarian Carcinoma.
REFERENCE   4  (bases 1 to 5322)
  AUTHORS   Gubas,A., Attridge,E., Jefferies,H.B., Nishimura,T., Razi,M.,
            Kunzelmann,S., Gilad,Y., Mercer,T.J., Wilson,M.M., Kimchi,A. and
            Tooze,S.A.
  TITLE     WIPI2b recruitment to phagophores and ATG16L1 binding are regulated
            by ULK1 phosphorylation
  JOURNAL   EMBO Rep 25 (9), 3789-3811 (2024)
   PUBMED   39152217
  REMARK    GeneRIF: WIPI2b recruitment to phagophores and ATG16L1 binding are
            regulated by ULK1 phosphorylation.
REFERENCE   5  (bases 1 to 5322)
  AUTHORS   Ghazi,P.C., O'Toole,K.T., Srinivas Boggaram,S., Scherzer,M.T.,
            Silvis,M.R., Zhang,Y., Bogdan,M., Smith,B.D., Lozano,G.,
            Flynn,D.L., Snyder,E.L., Kinsey,C.G. and McMahon,M.
  TITLE     Inhibition of ULK1/2 and KRASG12C controls tumor growth in
            preclinical models of lung cancer
  JOURNAL   Elife 13, 96992 (2024)
   PUBMED   39213022
  REMARK    GeneRIF: Inhibition of ULK1/2 and KRAS[G12C] controls tumor growth
            in preclinical models of lung cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5322)
  AUTHORS   Zhou,X., Babu,J.R., da Silva,S., Shu,Q., Graef,I.A., Oliver,T.,
            Tomoda,T., Tani,T., Wooten,M.W. and Wang,F.
  TITLE     Unc-51-like kinase 1/2-mediated endocytic processes regulate
            filopodia extension and branching of sensory axons
  JOURNAL   Proc Natl Acad Sci U S A 104 (14), 5842-5847 (2007)
   PUBMED   17389358
REFERENCE   7  (bases 1 to 5322)
  AUTHORS   Young,A.R., Chan,E.Y., Hu,X.W., Kochl,R., Crawshaw,S.G., High,S.,
            Hailey,D.W., Lippincott-Schwartz,J. and Tooze,S.A.
  TITLE     Starvation and ULK1-dependent cycling of mammalian Atg9 between the
            TGN and endosomes
  JOURNAL   J Cell Sci 119 (Pt 18), 3888-3900 (2006)
   PUBMED   16940348
  REMARK    GeneRIF: Starvation-induced autophagy, which requires mAtg9, may
            rely on an alteration of the steady-state trafficking of mAtg9, in
            a Atg1-dependent manner.
REFERENCE   8  (bases 1 to 5322)
  AUTHORS   Tomoda,T., Kim,J.H., Zhan,C. and Hatten,M.E.
  TITLE     Role of Unc51.1 and its binding partners in CNS axon outgrowth
  JOURNAL   Genes Dev 18 (5), 541-558 (2004)
   PUBMED   15014045
REFERENCE   9  (bases 1 to 5322)
  AUTHORS   Okazaki,N., Yan,J., Yuasa,S., Ueno,T., Kominami,E., Masuho,Y.,
            Koga,H. and Muramatsu,M.
  TITLE     Interaction of the Unc-51-like kinase and microtubule-associated
            protein light chain 3 related proteins in the brain: possible role
            of vesicular transport in axonal elongation
  JOURNAL   Brain Res Mol Brain Res 85 (1-2), 1-12 (2000)
   PUBMED   11146101
REFERENCE   10 (bases 1 to 5322)
  AUTHORS   Kuroyanagi,H., Yan,J., Seki,N., Yamanouchi,Y., Suzuki,Y.,
            Takano,T., Muramatsu,M. and Shirasawa,T.
  TITLE     Human ULK1, a novel serine/threonine kinase related to UNC-51
            kinase of Caenorhabditis elegans: cDNA cloning, expression, and
            chromosomal assignment
  JOURNAL   Genomics 51 (1), 76-85 (1998)
   PUBMED   9693035
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131009.7.
            
            On Sep 5, 2019 this sequence version replaced NM_003565.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF045458.1, AB018265.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000321867.6/ ENSP00000324560.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-491               AC131009.7         112273-112763
            492-584             AC131009.7         113252-113344
            585-626             AC131009.7         113434-113475
            627-659             AC131009.7         124543-124575
            660-696             AC131009.7         125146-125182
            697-870             AC131009.7         126295-126468
            871-944             AC131009.7         126549-126622
            945-1046            AC131009.7         126787-126888
            1047-1105           AC131009.7         127426-127484
            1106-1188           AC131009.7         127570-127652
            1189-1239           AC131009.7         127905-127955
            1240-1328           AC131009.7         128363-128451
            1329-1476           AC131009.7         129593-129740
            1477-1537           AC131009.7         130849-130909
            1538-1627           AC131009.7         131398-131487
            1628-1753           AC131009.7         132003-132128
            1754-1902           AC131009.7         132734-132882
            1903-1989           AC131009.7         132986-133072
            1990-2258           AC131009.7         133542-133810
            2259-2452           AC131009.7         134049-134242
            2453-2562           AC131009.7         134604-134713
            2563-2706           AC131009.7         135062-135205
            2707-2891           AC131009.7         136148-136332
            2892-3064           AC131009.7         136863-137035
            3065-3183           AC131009.7         137123-137241
            3184-3341           AC131009.7         137630-137787
            3342-3477           AC131009.7         138751-138886
            3478-5322           AC131009.7         138957-140801
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.33"
     gene            1..5322
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /note="unc-51 like autophagy activating kinase 1"
                     /db_xref="GeneID:8408"
                     /db_xref="HGNC:HGNC:12558"
                     /db_xref="MIM:603168"
     exon            1..491
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     CDS             381..3533
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /EC_number="2.7.11.1"
                     /note="ATG1 autophagy related 1 homolog; autophagy-related
                     protein 1 homolog"
                     /codon_start=1
                     /product="serine/threonine-protein kinase ULK1"
                     /protein_id="NP_003556.2"
                     /db_xref="CCDS:CCDS9274.1"
                     /db_xref="GeneID:8408"
                     /db_xref="HGNC:HGNC:12558"
                     /db_xref="MIM:603168"
                     /translation="MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVA
                     VKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLAD
                     YLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIR
                     VKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGK
                     APFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHP
                     FLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADT
                     AGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVAS
                     AGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQS
                     PTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQV
                     GTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVV
                     RPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSP
                     TKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPG
                     EDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPG
                     ARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAP
                     APELPAPGHGCSFADPITANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQH
                     VLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGL
                     QSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIH
                     SITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENV
                     TKCKLCIERRLSALLTGICA"
     exon            492..584
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            585..626
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            627..659
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            660..696
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            697..870
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            871..944
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1046
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1105
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1188
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1239
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1328
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1476
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1477..1537
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1627
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1753
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1902
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1903..1989
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2258
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2452
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2562
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2563..2706
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2891
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            2892..3064
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3065..3183
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3341
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3342..3477
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     exon            3478..5322
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5303..5308
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /note="hexamer: AATAAA"
     polyA_site      5322
                     /gene="ULK1"
                     /gene_synonym="ATG1; ATG1A; hATG1; UNC51; Unc51.1"
                     /note="major polyA site"
ORIGIN      
        1 agtgcggctg cgcgggcgtc tcaggctctg aggcccgggc gccgcggctc ttttgtttct
       61 ccgttggggc cgagcccggg ccctagttgg agccggagtc ggagtcggag tcggatccgg
      121 attcggatta gcagcccggg aagagtgccg tggcacaggc gccggaggga gcgcgaccct
      181 cggaccccgc ctggcccgcg gggctgggac ccggccccgg cctgcccgat ggggcgcgcg
      241 gccccggaga tgcgccctcg cccggccccg cgcccccggc cccgcgcccc cggcccgccc
      301 gccccggccc gcgcctccgc ctgagtcccc cgcgccttgg cccgccaccc cccgccccgc
      361 gcccccggcc cgcctgcgcc atggagcccg gccgcggcgg cacagagacc gtgggcaagt
      421 tcgagttctc ccgcaaggac ctgatcggcc acggcgcctt cgcggtggtc ttcaagggcc
      481 gccaccgcga gaagcacgat ttggaggtcg ccgtcaagtg cattaacaag aagaacctcg
      541 ccaagtctca gacgctgctg gggaaggaaa tcaaaatcct gaaggaactg aaacatgaaa
      601 acatcgtggc cctgtacgac ttccaggaaa tggctaattc tgtctacctg gttatggagt
      661 actgcaacgg tggggacctg gccgactacc tgcacgccat gcgcacgctg agcgaggaca
      721 ccatcaggct cttcctgcag cagatcgcgg gcgccatgcg gcttctgcac agcaaaggca
      781 tcatccaccg cgacctgaaa ccgcagaaca tcctgctgtc caaccccgcc ggccgccgcg
      841 ccaaccccaa cagcatccgc gtcaagatcg ctgacttcgg cttcgcgcgg tacctccaga
      901 gcaacatgat ggcggccaca ctctgcggct cccccatgta catggccccc gaggtcatca
      961 tgtcccagca ctacgacggg aaggcggacc tgtggagcat cggcaccatc gtctaccagt
     1021 gcctgacggg gaaggcgccc ttccaggcca gcagccccca ggacctgcgc ctgttctacg
     1081 agaagaacaa gacgttggtc cccaccatcc cccgggagac ctcggccccg ctgcggcagc
     1141 tgctcctggc cctactgcaa cgcaaccaca aggaccgcat ggacttcgat gagttttttc
     1201 atcacccttt cctcgatgcc agcccctcgg tcaggaaatc cccacccgtg cctgtgccct
     1261 cgtacccaag ctcggggtcc ggcagcagct ccagcagcag ctccacctcc cacctggcct
     1321 ccccgccgtc cctgggcgag atgcagcagc tgcagaagac cctggcctcc ccggctgaca
     1381 ccgctggctt cctgcacagc tcccgggact ctggtggcag caaggactct tcctgtgaca
     1441 cagacgactt cgtcatggtc cccgcgcagt ttccaggtga cctggtggct gaggcgccca
     1501 gtgccaaacc cccgccagac agcctgatgt gcagtgggag ctcactggtg gcctctgcgg
     1561 gcttggagag ccacggccgg accccatctc catccccacc ctgcagcagc tcccccagtc
     1621 cctcaggccg ggctggcccg ttctccagca gcaggtgcgg cgcctctgtc cccatcccag
     1681 tccccacgca ggtgcagaac taccagcgca ttgagcgaaa cctgcagtca cccacccagt
     1741 tccaaacacc tcggtcctct gccatccgca ggtcaggcag caccagcccc ctgggctttg
     1801 caagggccag cccctcgccc cctgcccacg ctgagcatgg aggcgtcctg gccaggaaga
     1861 tgtctctggg tggaggccgg ccctacacgc catctcctca agttggaacc atccctgagc
     1921 ggccaggctg gagcgggacg ccctccccac agggagctga gatgcggggt ggcaggtccc
     1981 ctcgtccagg ctcctctgca cccgagcact ctccccgcac ttccgggctg ggctgccgcc
     2041 tgcacagcgc ccccaacctg tctgacttgc acgtcgtccg ccccaagctg cccaaacccc
     2101 ccacggaccc cctgggagct gtgttcagcc caccacaggc cagccctccc cagccgtccc
     2161 acggcctgca gtcctgccgg aacctgcggg gctcacccaa gctgcccgac ttcctgcagc
     2221 gaaaccccct gccccccatc ctgggctccc ccaccaaggc tgtgccctcc tttgacttcc
     2281 cgaagacccc cagctcccag aacctgctgg ccctcctagc ccggcagggc gtggtgatga
     2341 cgccccctcg aaaccggacg ctgcccgacc tctcggaggt gggacccttc catggtcagc
     2401 cgttgggccc tggcctgcgg ccaggcgagg accccaaggg cccctttggc cggtctttca
     2461 gcaccagccg cctcactgac ctgctcctta aggcggcgtt tgggacacaa gccccggacc
     2521 cgggcagcac ggagagcctg caggagaagc ccatggagat cgcaccctca gctggctttg
     2581 gagggagcct gcacccagga gcccgtgctg ggggcaccag cagcccttcc ccggtggtct
     2641 tcaccgtggg ctctcccccg agcgggagca cgccccccca gggcccccgc accaggatgt
     2701 tctcagcggg ccccactggc tctgccagct cttctgcccg ccacctggtg cctgggccct
     2761 gcagcgaggc cccagcccct gagctccctg ctccaggaca cggctgcagc tttgccgacc
     2821 ccattactgc gaacctggag ggggctgtga ccttcgaggc ccccgacctc cctgaggaga
     2881 ccctcatgga gcaagagcac acggagatcc tgcgtggcct gcgcttcacg ctgctgttcg
     2941 tgcagcacgt cctggagatc gcagccctga agggcagcgc cagtgaggcg gcggggggcc
     3001 ctgagtacca gctgcaggag agtgtggtgg ccgaccagat cagcctgctg agccgagaat
     3061 ggggcttcgc ggaacagctg gtgctgtacc tgaaggtggc cgagctactg tcctccggcc
     3121 tgcaaagtgc catcgaccag atccgggccg gcaagctctg cctgtcgtcc actgtgaagc
     3181 aggtggtgcg caggctgaat gagctgtaca aggccagcgt ggtgtcctgc cagggcctga
     3241 gcctgcggct gcagcgcttc ttcctggaca agcagcggct cctggaccgc attcacagca
     3301 tcactgccga gaggctcatc ttcagccacg ctgtgcagat ggtgcagtcg gctgccctgg
     3361 acgagatgtt ccagcaccgt gagggctgcg tcccacgcta ccacaaggcc ctgctgctcc
     3421 tggaggggct gcagcacatg ctctcggacc aggccgacat cgagaacgtc accaagtgca
     3481 agctgtgcat tgagcggaga ctctcggcgc tgctgactgg catctgtgcc tgacctttct
     3541 ggcctggctg ggccccccgt cctgccgagc cctgcagagt gggctctgtg tgctggctgg
     3601 actcctcggg acaagcccat ggcgctgatc gctggtgctg agccctgccc tgggccccac
     3661 ggacagtcag cctgccggcc tccctgcagc tcacggggca gaaccagcac atctggagcc
     3721 acacagcttg gggggtgtct cccatctttt acaggtgggg atcacagaat ttctgcccct
     3781 ccagctgcct ggctcagcag gcgtgggtgc caccaccctc tagccccagg gcagccccgg
     3841 aggacaggca agggcctgag accactgccg actcaaagcc aaagcgagct cctgcttagg
     3901 gaaggtcagc aggcactgtg cccaggaaga gcctgcggcc tcggcgtccc ccagtctcca
     3961 ggagcctctc cctccgagat acccacccag ctttgtcaat cacccaagca ctttatgcat
     4021 atagagacag aacctggacc tcaccaggga ctgctgggca gcgattcctg gcagtggcct
     4081 ggtgtttgta catacacata tgcagacaca tgccagggcc ccccaagccc gagcaccgga
     4141 ccacgttgct gcccaggtct ggacctcagc gggagaactg gctccggggg gagtggggcc
     4201 ctgcgctaga ggcagaggca gttctttgtt caagcgttcc tctggggacc ggcagcagag
     4261 gcaccgtgtt ctctcagccc tggatacgtc ttgtaatctt tcacacttta ttcctaaaac
     4321 gtgtcttatt tttatgcagc tcattttttc tttaaaggag aaaacttgta ggtgtttaag
     4381 aattggtttt gggagggcga ggactgggcc aggttagagg cagatggcac aggggcgtgt
     4441 ggcgggcggg tgaggctgct ttgcacacct gtgttggtgg ctgtcccctg ccgcccctcc
     4501 ctgtggcagc agcaggacag gtgtgtgccc agcaccctcc ctacctgggc ctggaagcag
     4561 atgaggggaa tacttcatgc aaagaaaaaa gtaacatgtg caaaagctcc ccgtccagct
     4621 ttgacagtca gttttgatgt cagctcctcg gcagggtagg cctgatgaca gccctgtccc
     4681 tccctgcctc tgccttgccc aaggccacgg agggcatctg cagagaggcc tgccttccgg
     4741 attccaggcg ggcatgccct gcaaaccccg cctgggcctc ccttggtctg cccagccctc
     4801 gattagccct gcctgaatca gtagatactt gaacgagtcc ccagtctgcg ggaggcagtg
     4861 gtggggccat ggacccatgc ggggggttcc agggtcacac gccacataac agacaaaaat
     4921 acacacacgt gtgtttttct ttgcaatact tgaaatattg ccactgtgct tggacttaga
     4981 agaagaaaat ccccgtgact tcttcctcat caccttgatg gctttattct caccttgtgg
     5041 ggcatgtttg tatttattgc ttcatggccg actggaatcc tgagtcctgg gaagctggca
     5101 ctgcggggat cttgcccggt gtcctggtcc tcttgcttcc gtcgcggccg catgtgcgtg
     5161 tgtccaagca ggtcctgggc gcctcaactg ctgcccctgg ttgaatgttc tcttgatagt
     5221 gctggaccct ttgtctattt taaagcgaat tttgtgtgat ttcctgccct ttgcgttata
     5281 ttgtataata ccaacgtaag gaaataaacc tttggaattg tt
//
