LOCUS       NM_001075               2757 bp    mRNA    linear   PRI 08-MAR-2024
DEFINITION  Homo sapiens UDP glucuronosyltransferase family 2 member B10
            (UGT2B10), transcript variant 1, mRNA.
ACCESSION   NM_001075
VERSION     NM_001075.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Sipe,C.J., Koopmeiners,J.S., Donny,E.C., Hatsukami,D.K. and
            Murphy,S.E.
  TITLE     UGT2B10 Genotype Influences Serum Cotinine Levels and Is a Primary
            Determinant of Higher Cotinine in African American Smokers
  JOURNAL   Cancer Epidemiol Biomarkers Prev 29 (8), 1673-1678 (2020)
   PUBMED   32532831
  REMARK    GeneRIF: UGT2B10 Genotype Influences Serum Cotinine Levels and Is a
            Primary Determinant of Higher Cotinine in African American Smokers.
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Lu,D., Dong,D., Xie,Q., Li,Z. and Wu,B.
  TITLE     Disposition of Mianserin and Cyclizine in UGT2B10-Overexpressing
            Human Embryonic Kidney 293 Cells: Identification of UGT2B10 as a
            Novel N-Glucosidation Enzyme and Breast Cancer Resistance Protein
            as an N-Glucoside Transporter
  JOURNAL   Drug Metab Dispos 46 (7), 970-979 (2018)
   PUBMED   29691239
  REMARK    GeneRIF: UGT2B10 was for the first time identified as an
            N-glucosidation enzyme. Generated N-glucosides were excreted
            primarily by the BCRP transporter.
REFERENCE   3  (bases 1 to 2757)
  AUTHORS   Murphy,S.E., von Weymarn,L.B., Parenteau,M., Stepanov,I.,
            Tiirikainen,M., LeMarchand,L. and Park,S.L.
  TITLE     Influence of UGT2B10 Genotype on Urinary Excretion of
            4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol- N-glucuronide by
            African American Smokers
  JOURNAL   Chem Res Toxicol 31 (3), 168-175 (2018)
   PUBMED   29460622
  REMARK    GeneRIF: The high prevalence of a UGT2B10 splice variant among
            African Americans results in lower
            4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), -
            N-glucuronidation but only a small decrease in total NNAL
            glucuronidation. Therefore, despite the significant contribution of
            UGT2B10 to NNAL- N-glucuronidation, the UGT2B10 genotype does not
            play a large role in NNAL detoxication.
REFERENCE   4  (bases 1 to 2757)
  AUTHORS   Chen,G., Dellinger,R.W., Gallagher,C.J., Sun,D. and Lazarus,P.
  TITLE     Identification of a prevalent functional missense polymorphism in
            the UGT2B10 gene and its association with UGT2B10 inactivation
            against tobacco-specific nitrosamines
  JOURNAL   Pharmacogenet Genomics 18 (3), 181-191 (2008)
   PUBMED   18300939
  REMARK    GeneRIF: The UGT2B10(67Tyr) variant corresponding to haplotype C is
            a functional single nucleotide polymorphism that may be responsible
            for inter individual variation in NNAL-N-glucuronidation activity
            and may increase susceptibility to smoking-related cancers.
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   5  (bases 1 to 2757)
  AUTHORS   Chen,G., Blevins-Primeau,A.S., Dellinger,R.W., Muscat,J.E. and
            Lazarus,P.
  TITLE     Glucuronidation of nicotine and cotinine by UGT2B10: loss of
            function by the UGT2B10 Codon 67 (Asp>Tyr) polymorphism
  JOURNAL   Cancer Res 67 (19), 9024-9029 (2007)
   PUBMED   17909004
  REMARK    GeneRIF: Data suggest that UGT2B10 is the major hepatic enzyme
            involved in nicotine/cotinine glucuronidation and that the
            UGT2B10*2 variant reduces nicotine- and cotinine-N-glucuronidation
            formation and plays a role in nicotine metabolism and elimination.
REFERENCE   6  (bases 1 to 2757)
  AUTHORS   Kaivosaari,S., Toivonen,P., Hesse,L.M., Koskinen,M., Court,M.H. and
            Finel,M.
  TITLE     Nicotine glucuronidation and the human UDP-glucuronosyltransferase
            UGT2B10
  JOURNAL   Mol Pharmacol 72 (3), 761-768 (2007)
   PUBMED   17576790
  REMARK    GeneRIF: Nicotine glucuronidation and UGT2B10 is reported.
REFERENCE   7  (bases 1 to 2757)
  AUTHORS   Jin,P., Fu,G.K., Wilson,A.D., Yang,J., Chien,D., Hawkins,P.R.,
            Au-Young,J. and Stuve,L.L.
  TITLE     PCR isolation and cloning of novel splice variant mRNAs from known
            drug target genes
  JOURNAL   Genomics 83 (4), 566-571 (2004)
   PUBMED   15028279
REFERENCE   8  (bases 1 to 2757)
  AUTHORS   Strassburg,C.P., Kneip,S., Topp,J., Obermayer-Straub,P., Barut,A.,
            Tukey,R.H. and Manns,M.P.
  TITLE     Polymorphic gene regulation and interindividual variation of
            UDP-glucuronosyltransferase activity in human small intestine
  JOURNAL   J Biol Chem 275 (46), 36164-36171 (2000)
   PUBMED   10748067
REFERENCE   9  (bases 1 to 2757)
  AUTHORS   Strassburg,C.P., Strassburg,A., Nguyen,N., Li,Q., Manns,M.P. and
            Tukey,R.H.
  TITLE     Regulation and function of family 1 and family 2
            UDP-glucuronosyltransferase genes (UGT1A, UGT2B) in human
            oesophagus
  JOURNAL   Biochem J 338 (Pt 2) (Pt 2), 489-498 (1999)
   PUBMED   10024527
REFERENCE   10 (bases 1 to 2757)
  AUTHORS   Jin,C.J., Miners,J.O., Lillywhite,K.J. and Mackenzie,P.I.
  TITLE     cDNA cloning and expression of two new members of the human liver
            UDP-glucuronosyltransferase 2B subfamily
  JOURNAL   Biochem Biophys Res Commun 194 (1), 496-503 (1993)
   PUBMED   8333863
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC113649.1, AK292738.1 and AC021146.7.
            
            On Nov 23, 2018 this sequence version replaced NM_001075.5.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292738.1, BC113649.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145122, SAMEA2152798
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265403.12/ ENSP00000265403.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 BC113649.1         2-2
            2-1930              AK292738.1         9-1937
            1931-2757           AC021146.7         180435-181261       c
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q13.2"
     gene            1..2757
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /note="UDP glucuronosyltransferase family 2 member B10"
                     /db_xref="GeneID:7365"
                     /db_xref="HGNC:HGNC:12544"
                     /db_xref="MIM:600070"
     exon            1..744
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..1613
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /EC_number="2.4.1.17"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; UDP-glucuronosyltransferase 2B10; UDPGT 2B10;
                     UDP glucuronosyltransferase 2 family, polypeptide B10"
                     /codon_start=1
                     /product="UDP-glucuronosyltransferase 2B10 isoform 1
                     precursor"
                     /protein_id="NP_001066.1"
                     /db_xref="CCDS:CCDS75136.1"
                     /db_xref="GeneID:7365"
                     /db_xref="HGNC:HGNC:12544"
                     /db_xref="MIM:600070"
                     /translation="MALKWTTVLLIQLSFYFSSGSCGKVLVWAAEYSLWMNMKTILKE
                     LVQRGHEVTVLASSASILFDPNDSSTLKLEVYPTSLTKTEFENIIMQLVKRLSEIQKD
                     TFWLPFSQEQEILWAINDIIRNFCKDVVSNKKLMKKLQESRFDIVFADAYLPCGELLA
                     ELFNIPFVYSHSFSPGYSFERHSGGFIFPPSYVPVVMSKLSDQMTFMERVKNMLYVLY
                     FDFWFQIFNMKKWDQFYSEVLGRPTTLSETMRKADIWLMRNSWNFKFPHPFLPNVDFV
                     GGLHCKPAKPLPKEMEEFVQSSGENGVVVFSLGSMVSNMTEERANVIATALAKIPQKV
                     LWRFDGNKPDALGLNTRLYKWIPQNDLLGHPKTRAFITHGGANGIYEAIYHGIPMVGI
                     PLFFDQPDNIAHMKAKGAAVRVDFNTMSSTDLLNALKTVINDPSYKENIMKLSRIQHD
                     QPVKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWFQYHSLDVIGFLLACVATVLFIIT
                     KCCLFCFWKFARKGKKGKRD"
     exon            745..893
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1025
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1113
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1333
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..2757
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1827..1832
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /note="hexamer: AATAAA"
     polyA_site      1848
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /note="major polyA site"
     regulatory      2305..2310
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /note="hexamer: AATGAA"
     polyA_site      2329
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
     regulatory      2363..2368
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /note="hexamer: AGTAAA"
     regulatory      2372..2377
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /note="hexamer: ATTAAA"
     polyA_site      2385
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
     polyA_site      2400
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
     regulatory      2735..2740
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
                     /note="hexamer: AATAAA"
     polyA_site      2757
                     /gene="UGT2B10"
                     /gene_synonym="UDPGT2B10"
ORIGIN      
        1 gaaaagaatt atcacattgc acaaggatgg ctctgaaatg gactacagtt ctgctgatac
       61 aactcagttt ttactttagc tctgggagtt gtggaaaggt gctggtatgg gccgcagaat
      121 acagcctttg gatgaatatg aagacaatcc tgaaagaact tgttcagaga ggtcatgagg
      181 tgactgtact ggcatcttca gcttccattc tttttgatcc caacgactca tccactctta
      241 aacttgaagt ttatcctaca tctttaacta aaactgaatt tgagaatatc atcatgcaat
      301 tggttaagag attgtcagaa attcaaaaag atacattttg gttacctttt tcacaagaac
      361 aagaaatcct gtgggcaatt aatgacataa ttagaaactt ctgtaaagat gtagtttcaa
      421 ataagaaact tatgaaaaaa ctacaagagt caagatttga catcgttttt gcagatgctt
      481 atttaccctg tggtgagctg ctggctgagc tatttaacat accctttgtg tacagtcaca
      541 gcttcagtcc tggctactca tttgaaaggc acagtggagg atttattttc cctccttcct
      601 acgtacctgt tgttatgtca aaattaagtg atcaaatgac tttcatggag agggtaaaaa
      661 atatgctcta tgtgctttat tttgactttt ggttccaaat atttaatatg aagaagtggg
      721 atcagtttta cagtgaagtt ttaggaagac ccactacatt atctgagaca atgaggaaag
      781 ctgacatatg gcttatgcga aactcctgga attttaaatt tcctcatcca ttcttaccaa
      841 atgttgattt tgttggagga ctccactgca aacctgccaa acccctacct aaggaaatgg
      901 aggagtttgt acagagctct ggagaaaatg gtgttgtggt gttttctctg gggtcaatgg
      961 tcagtaacat gacagaagaa agggccaacg taattgcaac agcccttgcc aagatcccac
     1021 aaaaggttct ttggagattt gatgggaata aaccagatgc cttaggtctc aatactcgac
     1081 tgtacaagtg gataccccag aatgaccttc taggtcatcc aaaaaccaga gcttttataa
     1141 ctcatggtgg agccaatggc atctatgagg caatctacca tgggatccct atggtgggca
     1201 ttccattgtt ttttgatcaa cctgataata ttgctcacat gaaggccaag ggagcagctg
     1261 ttagagtgga cttcaacaca atgtcgagta cagacctgct gaatgcactg aagacagtaa
     1321 ttaatgatcc ttcatataaa gagaatatta tgaaattatc aagaattcaa catgatcaac
     1381 cagtgaagcc cctggatcga gcagtcttct ggattgaatt tgtcatgcgc cacaaaggag
     1441 ccaaacatct tcgagttgca gcccacaacc tcacctggtt ccagtaccac tctttggatg
     1501 tgattgggtt cctgctggct tgtgtggcaa ccgtgctatt tatcatcaca aagtgttgtc
     1561 tgttttgttt ctggaagttt gctagaaaag gaaagaaggg aaaaagggat tagttatatc
     1621 tgagatttga agctggaaaa cctgatagat aggaatactt cagttgattc cagcaataaa
     1681 tattgtgatg caagatttct ttcttcctgt gacaaaaaaa aatcctttcg aagtctacct
     1741 tgtcaagtaa aaatttgttt ttcagagatt taccacccag ttaatggtta gaaatattct
     1801 gtggcaatga agaaaacact aggggaaata aaaaataata taaagccata tgagcttgta
     1861 ttgaaatttg ttgcacttat attgaaatgt gatcatggct cacttcagcc tcaacttact
     1921 aagctcaaga ggttctctca cctcagcccc ccaagtagct gggaccatag gtgcatgtca
     1981 ccatgtccaa ctaatttttt attttttgta gtgatgagat ctcattgtgt tctccatgct
     2041 gatttcaaac tcctgggctc aaacaatcct cccattttag catcccaaag ggatgagatt
     2101 acaggtatgt accaccataa ctttacaaaa tgagattttt atataagaat gattcaaatg
     2161 ttcagggatg aaagagtcac taacataaaa gaagaatggg atgaggtgag aaggatgaat
     2221 acaaaaataa ttagatattc ttgaaatcag aaatgtgctc cctaattata tgaaatgttg
     2281 tttgattaca taaaataaag tggaaatgaa tgattgactg aacagcccac aagaagaatc
     2341 acttaatgct ctgaaaatta ccagtaaact gattaaaatc taaaattgct ttctgttaaa
     2401 gctttactga ttagtttttc ttccaaagct ctcttgtttc tagttgtttt cttggtctta
     2461 actacccatt atatgctttg ttaaagtgtt tatgccctga ttcaatgtga ttatctcaat
     2521 ttttatttca ttctgtccta actcttgcaa cctgcatgtc ctctttatta ttgatcaatc
     2581 caactgcaaa gttcacctta cctgactaag gattattcat taagttttac ttgtttatct
     2641 gacatttatt attttgtctc tttgctagtc actctgagcc atggtcatga tgacttagga
     2701 ttctggatct cttatgaata acaaatttat ccttaataaa gtctctatac taaagaa
//
