LOCUS       NM_020120              10761 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens UDP-glucose glycoprotein glucosyltransferase 1
            (UGGT1), transcript variant 1, mRNA.
ACCESSION   NM_020120
VERSION     NM_020120.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Tax,G., Guay,K.P., Pantalone,L., Ceci,M., Solda,T., Hitchman,C.J.,
            Hill,J.C., Vasiljevic,S., Lia,A., Modenutti,C.P., Straatman,K.R.,
            Santino,A., Molinari,M., Zitzmann,N., Hebert,D.N., Roversi,P. and
            Trerotola,M.
  TITLE     Rescue of secretion of rare-disease-associated misfolded mutant
            glycoproteins in UGGT1 knock-out mammalian cells
  JOURNAL   Traffic 25 (1), e12927 (2024)
   PUBMED   38272446
  REMARK    GeneRIF: Rescue of secretion of rare-disease-associated misfolded
            mutant glycoproteins in UGGT1 knock-out mammalian cells.
REFERENCE   2  (bases 1 to 10761)
  AUTHORS   Sagert,L., Winter,C., Ruppert,I., Zehetmaier,M., Thomas,C. and
            Tampe,R.
  TITLE     The ER folding sensor UGGT1 acts on TAPBPR-chaperoned peptide-free
            MHC I
  JOURNAL   Elife 12, e85432 (2023)
   PUBMED   37345806
  REMARK    GeneRIF: The ER folding sensor UGGT1 acts on TAPBPR-chaperoned
            peptide-free MHC I.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10761)
  AUTHORS   Jia,W., Zou,X., Xu,Z., Bai,L., Shan,A., Li,Y., Shi,J., Yang,F.,
            Ding,C., Narimatsu,H. and Zhang,Y.
  TITLE     Polypeptide N-acetylgalactosaminyltransferase 18 retains in
            endoplasmic reticulum depending on its luminal regions interacting
            with ER resident UGGT1, PLOD3 and LPCAT1
  JOURNAL   Glycobiology 31 (8), 947-958 (2021)
   PUBMED   33909026
  REMARK    GeneRIF: Polypeptide N-acetylgalactosaminyltransferase 18 retains
            in endoplasmic reticulum depending on its luminal regions
            interacting with ER resident UGGT1, PLOD3 and LPCAT1.
REFERENCE   4  (bases 1 to 10761)
  AUTHORS   Adams,B.M., Canniff,N.P., Guay,K.P., Larsen,I.S.B. and Hebert,D.N.
  TITLE     Quantitative glycoproteomics reveals cellular substrate selectivity
            of the ER protein quality control sensors UGGT1 and UGGT2
  JOURNAL   Elife 9, e63997 (2020)
   PUBMED   33320095
  REMARK    GeneRIF: Quantitative glycoproteomics reveals cellular substrate
            selectivity of the ER protein quality control sensors UGGT1 and
            UGGT2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10761)
  AUTHORS   Wang,N., Seko,A., Takeda,Y. and Ito,Y.
  TITLE     Glycan dependent refolding activity of ER glucosyltransferase
            (UGGT)
  JOURNAL   Biochim Biophys Acta Gen Subj 1864 (12), 129709 (2020)
   PUBMED   32858085
  REMARK    GeneRIF: Glycan dependent refolding activity of ER
            glucosyltransferase (UGGT).
REFERENCE   6  (bases 1 to 10761)
  AUTHORS   Caramelo,J.J. and Parodi,A.J.
  TITLE     Getting in and out from calnexin/calreticulin cycles
  JOURNAL   J Biol Chem 283 (16), 10221-10225 (2008)
   PUBMED   18303019
  REMARK    Review article
REFERENCE   7  (bases 1 to 10761)
  AUTHORS   Arnold,S.M. and Kaufman,R.J.
  TITLE     The noncatalytic portion of human UDP-glucose: glycoprotein
            glucosyltransferase I confers UDP-glucose binding and transferase
            function to the catalytic domain
  JOURNAL   J Biol Chem 278 (44), 43320-43328 (2003)
   PUBMED   12913004
  REMARK    GeneRIF: the amino-terminal 80% of HUGT1 is required for activation
            of the catalytic domain
REFERENCE   8  (bases 1 to 10761)
  AUTHORS   Aoki,K., Ishida,N. and Kawakita,M.
  TITLE     Substrate recognition by nucleotide sugar transporters: further
            characterization of substrate recognition regions by analyses of
            UDP-galactose/CMP-sialic acid transporter chimeras and biochemical
            analysis of the substrate specificity of parental and chimeric
            transporters
  JOURNAL   J Biol Chem 278 (25), 22887-22893 (2003)
   PUBMED   12682060
  REMARK    GeneRIF: The substrate binding specificity
REFERENCE   9  (bases 1 to 10761)
  AUTHORS   Korotkov,K.V., Kumaraswamy,E., Zhou,Y., Hatfield,D.L. and
            Gladyshev,V.N.
  TITLE     Association between the 15-kDa selenoprotein and
            UDP-glucose:glycoprotein glucosyltransferase in the endoplasmic
            reticulum of mammalian cells
  JOURNAL   J Biol Chem 276 (18), 15330-15336 (2001)
   PUBMED   11278576
REFERENCE   10 (bases 1 to 10761)
  AUTHORS   Arnold,S.M., Fessler,L.I., Fessler,J.H. and Kaufman,R.J.
  TITLE     Two homologues encoding human UDP-glucose:glycoprotein
            glucosyltransferase differ in mRNA expression and enzymatic
            activity
  JOURNAL   Biochemistry 39 (9), 2149-2163 (2000)
   PUBMED   10694380
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB226703.1, AF227905.1, AC108059.6, BC041098.1, AC017079.5 and
            AK025416.1.
            
            On May 17, 2019 this sequence version replaced NM_020120.3.
            
            Summary: UDP-glucose:glycoprotein glucosyltransferase (UGT) is a
            soluble protein of the endoplasmic reticulum (ER) that selectively
            reglucosylates unfolded glycoproteins, thus providing quality
            control for protein transport out of the ER.[supplied by OMIM, Oct
            2009].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript but encodes the functional protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.208741.1, AF227905.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000259253.11/ ENSP00000259253.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-120               DB226703.1         18-137
            121-829             AF227905.1         1-709
            830-830             AC108059.6         82700-82700
            831-1114            AF227905.1         711-994
            1115-1115           AC108059.6         89906-89906
            1116-3709           AF227905.1         996-3589
            3710-6505           BC041098.1         3880-6675
            6506-9560           AC017079.5         37936-40990
            9561-10761          AK025416.1         842-2042
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q14.3"
     gene            1..10761
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="UDP-glucose glycoprotein glucosyltransferase 1"
                     /db_xref="GeneID:56886"
                     /db_xref="HGNC:HGNC:15663"
                     /db_xref="MIM:605897"
     exon            1..216
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..4826
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="UDP-glucose ceramide glucosyltransferase-like 1;
                     UDP--Glc:glycoprotein glucosyltransferase"
                     /codon_start=1
                     /product="UDP-glucose:glycoprotein glucosyltransferase 1
                     precursor"
                     /protein_id="NP_064505.1"
                     /db_xref="CCDS:CCDS2154.1"
                     /db_xref="GeneID:56886"
                     /db_xref="HGNC:HGNC:15663"
                     /db_xref="MIM:605897"
                     /translation="MGCKGDASGACAAGALPVTGVCYKMGVLVVLTVLWLFSSVKADS
                     KAITTSLTTKWFSTPLLLEASEFLAEDSQEKFWNFVEASQNIGSSDHDGTDYSYYHAI
                     LEAAFQFLSPLQQNLFKFCLSLRSYSATIQAFQQIAADEPPPEGCNSFFSVHGKKTCE
                     SDTLEALLLTASERPKPLLFKGDHRYPSSNPESPVVIFYSEIGSEEFSNFHRQLISKS
                     NAGKINYVFRHYIFNPRKEPVYLSGYGVELAIKSTEYKAKDDTQVKGTEVNTTVIGEN
                     DPIDEVQGFLFGKLRDLHPDLEGQLKELRKHLVESTNEMAPLKVWQLQDLSFQTAARI
                     LASPVELALVVMKDLSQNFPTKARAITKTAVSSELRTEVEENQKYFKGTLGLQPGDSA
                     LFINGLHMDLDTQDIFSLFDVLRNEARVMEGLHRLGIEGLSLHNVLKLNIQPSEADYA
                     VDIRSPAISWVNNLEVDSRYNSWPSSLQELLRPTFPGVIRQIRKNLHNMVFIVDPAHE
                     TTAELMNTAEMFLSNHIPLRIGFIFVVNDSEDVDGMQDAGVAVLRAYNYVAQEVDDYH
                     AFQTLTHIYNKVRTGEKVKVEHVVSVLEKKYPYVEVNSILGIDSAYDRNRKEARGYYE
                     QTGVGPLPVVLFNGMPFEREQLDPDELETITMHKILETTTFFQRAVYLGELPHDQDVV
                     EYIMNQPNVVPRINSRILTAERDYLDLTASNNFFVDDYARFTILDSQGKTAAVANSMN
                     YLTKKGMSSKEIYDDSFIRPVTFWIVGDFDSPSGRQLLYDAIKHQKSSNNVRISMINN
                     PAKEISYENTQISRAIWAALQTQTSNAAKNFITKMAKEGAAEALAAGADIAEFSVGGM
                     DFSLFKEVFESSKMDFILSHAVYCRDVLKLKKGQRAVISNGRIIGPLEDSELFNQDDF
                     HLLENIILKTSGQKIKSHIQQLRVEEDVASDLVMKVDALLSAQPKGDPRIEYQFFEDR
                     HSAIKLRPKEGETYFDVVAVVDPVTREAQRLAPLLLVLAQLINMNLRVFMNCQSKLSD
                     MPLKSFYRYVLEPEISFTSDNSFAKGPIAKFLDMPQSPLFTLNLNTPESWMVESVRTP
                     YDLDNIYLEEVDSVVAAEYELEYLLLEGHCYDITTGQPPRGLQFTLGTSANPVIVDTI
                     VMANLGYFQLKANPGAWILRLRKGRSEDIYRIYSHDGTDSPPDADEVVIVLNNFKSKI
                     IKVKVQKKADMVNEDLLSDGTSENESGFWDSFKWGFTGQKTEEVKQDKDDIINIFSVA
                     SGHLYERFLRIMMLSVLKNTKTPVKFWFLKNYLSPTFKEFIPYMANEYNFQYELVQYK
                     WPRWLHQQTEKQRIIWGYKILFLDVLFPLVVDKFLFVDADQIVRTDLKELRDFNLDGA
                     PYGYTPFCDSRREMDGYRFWKSGYWASHLAGRKYHISALYVVDLKKFRKIAAGDRLRG
                     QYQGLSQDPNSLSNLDQDLPNNMIHQVPIKSLPQEWLWCETWCDDASKKRAKTIDLCN
                     NPMTKEPKLEAAVRIVPEWQDYDQEIKQLQIRFQKEKETGALYKEKTKEPSREGPQKR
                     EEL"
     exon            217..352
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            353..435
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            436..566
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            567..679
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            680..854
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            855..951
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1030
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1131
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1231
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1292
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1384
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1535
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1655
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1741
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1877
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..2009
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2174
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2295
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2394
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2418
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2513
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2514..2720
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2721..2852
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2853..2983
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            2984..3079
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3080..3182
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3183..3262
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3263..3452
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3453..3611
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3612..3697
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3698..3782
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3783..3871
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3872..3973
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            3974..4058
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            4059..4241
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            4242..4402
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            4403..4517
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            4518..4634
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            4635..4800
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     exon            4801..10761
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4875..4880
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="hexamer: AATAAA"
     polyA_site      4896
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="major polyA site"
     regulatory      7569..7574
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="hexamer: TATAAA"
     polyA_site      7595
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="major polyA site"
     regulatory      10710..10715
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="hexamer: AATGAA"
     regulatory      10716..10721
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="hexamer: AATAAG"
     regulatory      10735..10740
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="hexamer: AGTAAA"
     polyA_site      10735
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="major polyA site"
     polyA_site      10740
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
     polyA_site      10761
                     /gene="UGGT1"
                     /gene_synonym="HUGT1; UGCGL1; UGT1"
                     /note="major polyA site"
ORIGIN      
        1 agctgggcaa ttgctttgcg aggctgggtg ttgagtcgag ccgcgggaaa ggcgcgtgtc
       61 ggcctctcac tggcgcagcc tgcactgccg ctgccgcctc gccccgccct gccctggcgt
      121 tgtctctggc actgtggcgg actgaccacg gcccgggcat gggctgcaag ggagacgcga
      181 gcggtgcgtg tgccgcgggt gcgctgccgg tgacaggagt ttgctataaa atgggagttc
      241 tggttgtact cactgttctg tggctgttct cctcagtaaa ggccgactca aaagccatta
      301 caacctctct tacaacaaaa tggttttcca ctccattgtt gttagaagcc agtgagtttt
      361 tagcagaaga cagtcaagag aaattttgga attttgtaga agccagtcaa aatattggat
      421 catcagatca tgacggtacc gattattcct actatcatgc aatattggag gctgcatttc
      481 agtttctgtc acccctccag cagaatttgt ttaaattttg tctgtccctt cgttcttact
      541 cagctacaat ccaagccttc cagcagatag cagctgatga acctccacca gaaggatgta
      601 attcgttttt ttcagtgcat ggaaagaaga cttgtgaatc tgataccctt gaggctcttc
      661 tactgacagc ctctgaaaga cccaaacctt tattgttcaa aggagatcac agatatccct
      721 cgtctaatcc tgaaagccct gtggtgattt tctactctga gattggctct gaggaatttt
      781 ccaattttca ccgccagctt atatcaaaaa gcaatgcagg caaaatcaat tatgtattca
      841 gacattatat atttaatccc aggaaggagc ctgtttacct ctctggctat ggcgtggaat
      901 tggccattaa gagcactgag tacaaggcca aggatgatac tcaggtgaaa ggaactgagg
      961 taaacaccac agtgattggt gaaaatgatc ctattgatga ggttcagggg ttcctctttg
     1021 gaaaattaag agatctgcac cccgacctgg agggacagtt gaaagaactc agaaagcatc
     1081 ttgtagagag caccaatgaa atggcacctt taaaggtttg gcagttgcaa gatctcagtt
     1141 tccagactgc tgctcgaatc ttggcttctc ctgttgagtt ggctttggtt gtcatgaagg
     1201 atcttagtca gaattttcct accaaagcca gagcaataac aaaaacagct gtgagctcag
     1261 aacttagaac cgaagtggaa gagaatcaga agtatttcaa gggaacttta ggattacaac
     1321 ctggagattc agccctcttc atcaatggac ttcacatgga tttagataca caggatatat
     1381 tcagtctgtt tgatgtgttg aggaatgaag ctcgggtaat ggagggtctg catagattgg
     1441 gaatagaagg cctttctctg cataatgttt tgaagctgaa catccagccc tctgaggcag
     1501 actatgccgt agacatccgg agtcctgcta tttcatgggt caacaacctg gaggttgata
     1561 gcagatataa ttcgtggcct tctagtttac aagagttgct tcgacccacc tttcctggtg
     1621 ttattcggca gatcaggaaa aacttacata atatggtttt catagttgat cctgcacatg
     1681 agaccacagc agagttgatg aacacagctg agatgttcct tagtaatcat ataccactaa
     1741 gaattggttt tatctttgtg gttaatgact ctgaagatgt tgatgggatg caagatgctg
     1801 gagtggctgt tcttagagca tataattatg ttgcccaaga agtggatgat tatcatgcct
     1861 tccagactct gacacatatc tataacaagg tgaggactgg agaaaaagtg aaagttgaac
     1921 atgtggtcag tgtcctggag aagaaatatc cgtatgtaga agtgaatagc attttgggga
     1981 ttgattctgc ttatgatcgg aatcggaagg aagcaagagg ctactatgag cagactggag
     2041 ttggacctct gcccgttgtg ctgttcaatg gaatgccctt tgaaagggaa cagctagacc
     2101 ctgatgagtt agaaaccatc acaatgcata aaatcctgga gaccaccacc ttcttccaaa
     2161 gagcggtgta cttgggtgaa ctgccccatg atcaagatgt ggtagagtat atcatgaatc
     2221 agccaaatgt tgttccacga atcaattcta ggattttgac agctgaacga gactacctgg
     2281 atttaacagc gagtaataac ttctttgtgg atgattatgc tagatttact atcttggatt
     2341 cccaaggcaa gactgctgct gtagccaata gtatgaacta tctgacaaag aaaggaatgt
     2401 cctccaagga aatctatgat gattctttta ttaggccagt aactttttgg attgttgggg
     2461 attttgatag cccttctgga cggcagttac tgtatgatgc catcaaacat cagaaatcca
     2521 gtaacaatgt tagaataagc atgatcaata atcctgccaa agagataagc tatgagaaca
     2581 ctcagatctc cagagcaatc tgggcagctc tccaaactca gacttccaac gctgctaaga
     2641 acttcatcac caaaatggcc aaggaggggg ctgcagaggc cctggctgca ggagctgaca
     2701 ttgcggagtt ctctgttggg ggaatggatt tcagtctttt taaagaggtc tttgagtctt
     2761 ccaaaatgga tttcattttg tctcatgccg tgtactgcag ggatgttctg aagctgaaga
     2821 agggacagag ggcagtgatc agcaatggaa ggatcattgg gccactggag gatagtgagc
     2881 tctttaatca agacgatttc cacctcctcg aaaatatcat cttaaaaacc tcaggacaga
     2941 aaataaaatc tcatattcaa cagcttcggg tagaagaaga tgtggcaagc gacttggtaa
     3001 tgaaggtgga tgctcttctg tcagcgcaac caaaaggaga tccaagaatc gagtaccagt
     3061 tttttgaaga cagacacagt gcaatcaaac tgaggccgaa ggaaggggag acatactttg
     3121 atgttgtggc tgtcgttgac cctgtcacca gagaagcaca gagacttgct cctttgctct
     3181 tggttttggc tcagctgata aacatgaatc tgagagtatt tatgaactgc caatccaaac
     3241 tttctgacat gcctttaaaa agcttttacc gttatgtctt agaaccagag atttctttca
     3301 cttcagacaa tagttttgct aagggtccaa tcgcaaaatt tttggatatg cctcagtctc
     3361 cactgttcac tctgaatttg aacacacctg agagctggat ggtagaatct gtcagaacac
     3421 catatgatct tgataatatt tatttagaag aggtggacag tgtagtggct gctgagtatg
     3481 agctggaata cctgttactg gaaggtcatt gctacgacat caccacaggc cagcctccac
     3541 ggggactaca gtttacctta ggaacttcag ccaacccggt cattgtggac accattgtta
     3601 tggccaatct gggctacttt cagctgaaag ccaacccagg agcttggatc ctcagactta
     3661 ggaagggacg ctctgaagat atttatagaa tttacagcca cgatggcact gattctcccc
     3721 ctgatgctga tgaggtggtt atcgtcctca acaacttcaa aagcaaaatt attaaagtga
     3781 aggttcagaa gaaggcagat atggtgaacg aagacttgct gagtgatgga acgagtgaga
     3841 atgaatctgg attttgggat tccttcaaat ggggctttac aggacagaag actgaggaag
     3901 tgaagcaaga taaagatgac ataattaata ttttctccgt tgcatctggt catctctacg
     3961 aaagatttct tcgcataatg atgctatccg tgctgaagaa taccaagact cctgtgaaat
     4021 tctggttctt gaagaattac ttgtccccca catttaagga gtttatacct tacatggcaa
     4081 atgaatacaa tttccagtat gagcttgttc agtacaaatg gccccggtgg cttcatcaac
     4141 aaactgaaaa acagcgtatc atctggggtt acaagatcct cttcctggat gtacttttcc
     4201 cactagttgt tgacaagttc ctgtttgtgg atgctgatca gattgtacga acagatctga
     4261 aagagttaag agatttcaat ttggatggtg ctccttatgg ttacactcct ttctgtgaca
     4321 gccgaagaga aatggacggc tacaggttct ggaagtcagg gtactgggcc agtcatttag
     4381 ccgggcgaaa gtatcatatc agtgcactat atgttgtgga tctgaagaag tttaggaaaa
     4441 tagctgctgg tgacagactc aggggacagt accaaggtct gagtcaggac cctaacagcc
     4501 tttcaaatct tgatcaagat ctgcccaata acatgattca tcaggtgcca attaaatccc
     4561 tccctcaaga atggctttgg tgtgaaacgt ggtgtgatga cgcctctaag aaaagggcaa
     4621 aaaccattga tttgtgtaat aatccgatga ccaaagagcc gaaactggaa gcagctgtgc
     4681 ggattgtccc ggagtggcag gactacgacc aagagatcaa acagctacag atccgctttc
     4741 agaaggagaa agaaacggga gcactgtaca aagagaagac aaaagaacca agccgagaag
     4801 gtcctcagaa acgtgaagaa ttatgatctc tggagaagga caggaaatca ccccatttga
     4861 aaaacagttt ttataataaa tgctagtttt ttctgatctg tctatacaac tgctgataag
     4921 ccggctgggc aggagtgcca caccttttga ttctgagcat ttgattctga cttctgtact
     4981 ctggtggcca ctggatcttt gggattaaag ctctgttgga tttgtacctc agaggaagac
     5041 caagtggctg atcctttgga ctctgtaaag agcattcttc tagtcagagg gtggaatggc
     5101 agcagcaact ggaagaaaat gagttttttg gtgcccacac ccaagagcac acacatgctg
     5161 cactgtctcg gaaagcaggg ccagctagag ccaccatgtt cttccttacc tcagtttacc
     5221 tgcggcctgc gctgcactgc agatgcccac cctgccctgg gtctggccgg cggaagctct
     5281 gtccaaggtc cacacacctc caggtttacg ccaacatcct tgtgccctcc ccaccttctc
     5341 ttccaacgca ttaggtgcat tgtttaattg aaatccaacc aacaattgtg tgtcaaggct
     5401 ggtttggtgc agtggctggg caaattaatt ttgggccagg atgggggtgg gttgcagtga
     5461 gggtagggaa aatgtcagga gtaggaaggt tcgggggtta agggaaggga aggaagacca
     5521 gaactggcca tcctctttta taatccatta gtagcaccat ggctcatttg aaatgaaaat
     5581 attacactta ttccccaccc aaccgcagtg aactttctag gtaattgttt tgaaaacaat
     5641 ttttgtatct gtgaaagtct ttgctttttc tttccacctt ctagaaaagt ctgctaccag
     5701 tttccttact gaatacagcc atactcagcc cctctcgcat ccagcccgtc agggtcaggg
     5761 tcagggtcag gctccctcaa gacgagcacc gcattgtctg ccctcttttg cgtaggattt
     5821 ttctctcaga cccaggggac attgccttgg acttttctct acaaatgccc tcagatgtaa
     5881 gaccagaaat gattctgctt gtggaactcg tggctttctg tcctattttc cttttatgtg
     5941 agccctgcag gttgcctttg gcgcccacac cagttctgtc ttcatgtcgc tgcatcgtga
     6001 agccccattt gcctttagtc cctgctttgt gtgtcttcac ttccacagcc agtgttgccc
     6061 ctcagggcat actttgctgg tgaacgggct gtagagtctt gagtatgtta ttggcctaag
     6121 tgggcatgag gccccagcat ccgactccat ctgccaggct gcagggcaca gcagcactgg
     6181 catagacagg cacgctctgt cttccaacgt gcaccagcct tttcttacat attttgcctg
     6241 tttttaaaaa atacgaggag agggtgcaga tggatactgt gccctcaccc tcctaagcca
     6301 ggtaatatat tgatgtgcag acttttaaga aatcttagtc ttatcacaaa tatcaagtaa
     6361 tttatttttt ccattttcaa atgcaaaagt tagcctagga agcctcccag tcatcctgaa
     6421 ggaccttttg actttgcttt tataattctg ttaaaaattg aggaggggat taatgagtac
     6481 aatgaaaaca aaacaaaaca aaaaattaag aagaggaaag ctcaaaagag gataagagac
     6541 tgaaaaggca cctcagaatg gctgctgtgg gcagagtctg tgacatgact ttccccgccc
     6601 tgcccctctg ttcagagcag ctggaaagat gggatcagta agattaaaaa acttttgttc
     6661 ggccaggcgt ggtggctcac acctgtaatc ccagcacttt gggaggctga ggcggacgga
     6721 tcatgaggtc aggagttcaa gaccagcctg gccaatatgg tgaaaccccg tctctactaa
     6781 aaatacaaaa attagccggg tgttgtggct caccctctat tcccagctac tcgggaggca
     6841 gagacaggag aatcgcctga acccaggagg cagaggttgc agtgagctga gatcgtgcca
     6901 ctgcactcca gcctgggtga cagagcgaga ctccatctca gaaaacaaac aaacaaaaag
     6961 aaacttttgt tcaagctgca tcttcacatt gaatgaaagc attcagacgg gtgactgctg
     7021 ctgtccacaa ctgtgggcgg aagtgtgcct tttcccagtt tctcgtccag gaggttgcat
     7081 agcaaatact caagtcactg ggatatatgc tttccaaatg ctggccccca tagcaccttt
     7141 ccccatgtct ttctaccaag cctcttatgt ttaatctgtg cagggaagac aagagtagac
     7201 catattctct gtgtgctgtg catgtcctgg tgtcactcat tgcctcttat ctgtcgccat
     7261 tctaatataa gtcagcttaa tagttgactc tgaataatgc aaaacccagc ttgcttccag
     7321 aatggcctag gaccctcctc ctcctgccag tttttttttt tttttttggt tcatgtggca
     7381 taatcatctt tcaaaattaa ccaaatgccc cagaaaatga tgttaccact aaaatatttt
     7441 ctccaagaag gcatactgta acagaaaaga taggtaagat atttttttcc caagaagtaa
     7501 gaattgattg tgctgaatgt tcaaactctg gaaactgttc acagtatgtt gttcttagct
     7561 gttatttata taaagtgaat tattttctct ctcaatcatg ccatagtgga gacttttgtt
     7621 tgtgagtaag tgacagagta atatgaaata atgtgatatg tcagagtgac atgcagcatc
     7681 ctgagcctgt ttttatgttc tgtgtacagt ggcatttgat tgagatcaaa gcctgtcaca
     7741 gcctgctgct gggggtgtgg ggatatgcca gagtcctgtg tttggtttta gaagtttact
     7801 gaatgtttcc cacatggaga ataatggatg gcatgttagt ttctttaaga gtggtaaagt
     7861 tgtacatctg tcattgttgc atttcgaatt gaacacatag atgcttgcct gtctgcttgg
     7921 gtgaatgcct cttgggagag cgaagattac gtgcatgatg tatgttaaga aacagtttcc
     7981 ggtcaggcac agtggcttac acctgtaatc ccagcacttt gggaagccga ggcgggcgga
     8041 ttatgaggtc aagagataga gaccatcctg gccaacacgg tgaaaccccg tctctactaa
     8101 aaatacaaaa attagctggg catggtggca cacacctgta gtcccagcta ctcgggaggc
     8161 tgaggcagga gaatcacttg aacccgggag gtggaggttg cagtgagccg agatcatgcc
     8221 actgcactcc agcctggcaa tagagcgaga ctccgtctca aaaaaaaaaa aaaaaaaaaa
     8281 aaaaaaatta aaacagtttc cataaatgga gcttaatttg ctctgctgtc cttaagccct
     8341 cgtgggtttt tttttaagag agtctcattc tgtcacccag gctggagtgc agtggtgcga
     8401 tcttggctca cttcaacctc tgcctcccaa gttcaaacaa ttctcctgcc tcagcctcct
     8461 gagtagctgg gattacaggc gcctgccacc atgcctggtt aatttttgta tttttagtag
     8521 agatggggtt ttaccatgtt ggttggccag gctgatctca aactcctgac ctcaggtggt
     8581 gatctgccca cctcggcctc ccaaagtgct gggattacag gcgtgagcca ctatgtccgg
     8641 tcaagccctc gtgttctgaa ccttgtcaag gctctgcctg aatgagtttt catctgattg
     8701 tggggaccaa gtccctgagt agagggccaa gagctaggga cagggggaag agactggccc
     8761 aggtggtagg gaggaaagaa ctcccagagt ttcctttagc caggaaacct gctctactga
     8821 ccccgtgact tggacagtca gacatcaccc tgagagtgac aagtgtaaaa tgactccctt
     8881 cctcccccgc cctccggaag tatatttaga tacttgaaag cagtcctttt ctaaaatggc
     8941 cttacctatg tggcctgaac gattaaaaga aagaactcag agttacaagg gaaaaagaaa
     9001 aagagttaca agggaattgt agtctttttc tgaatagaat attagtactg tggtattgca
     9061 tttcatggga atggaaatgt attggtaaag ctacctgatg gaagctttct cttggtaaca
     9121 aaaatggagg gtgtattatg tgcagttatt taaatatatg tattccattg ttgcgtggtt
     9181 tttttccccc ctcaagacag agatttgctc ttgttgccca gggtggagta caatggtgtg
     9241 accttggctc actgcaacct ctgcctcctg ggttcaggtg attctcctgc ctcagccttc
     9301 cgagtagctg ggattacagg tgtgcgccac cacacccggc taatttttgc tttttttttt
     9361 tttgagacag aatctcgctc tgttgctcag gctggagtgg agtgcagtgg cacaatctca
     9421 gctcactgca gcctctgcct cccgggttca agagattctc ctgactcaac ctcctgagta
     9481 gctgggatta caggtgcctg ccactatgcc tagctaattt ttgtattttt agtagagacg
     9541 gggttttgcc atgttggcca gggtggtctc gaactcctga cctcaagtga tccacccgca
     9601 tcggcccccc aaagtgctgg gattataggc gtgaaccacc gcgcctgtcc cattgttgtg
     9661 taattttaat aattagtttt ttaagtactt gattttatgg gcacattttt gtgggatgat
     9721 tggagttaat caaataaagc ttgtcatgtg tgtagtttgg taagataact tctttaaatt
     9781 catgttttct ctgccttgag gtagtgaggg aaagatctta atcagtattt tggtaattaa
     9841 ctgattgaat tcaagcaaat gagacatcat gaacttcagt ggttattgat atttcagggt
     9901 atatacctga aatgcctaga ggatacagat ttctcatttc attctttggt ctttcatttc
     9961 tctatataca gaaatgaaat gacacttctg ggaggcagta gaagcaggaa gtcaatgaat
    10021 tgagtagagg gtcccattcc ctcaggctgt cattgatcag tgacaattta taaaaacaaa
    10081 ctgcaaagtc tgtggcaagt ggctgcctgc ttcctagaag gagcccatga aggttaaact
    10141 ctgtggtcgg tatttgcaag cgccgggcgt ggtggctcac gcctgtaatc ctagcacctt
    10201 gggaggccaa ggcaggcgga tcacccgagg tcaggagttt gaggatttgc aagcaaaagg
    10261 tcctctcctg agtctttccc agatacccag cagtgcagag gctagctgtg gaaggttgca
    10321 gtgggacagg aatgtattgt atgccttgcc ttacttgtca ccattgagat ttccagagaa
    10381 atgggcataa cgtctcttaa caacaacagc agaaagcaaa atacattaac ttaaggttga
    10441 caacaaaaga ttatcaagta ccatgttttc caaccaacca gttattcgtg gtaataataa
    10501 aataaaggtg ggaaaatgtt ataattttta aggaaactgt gtactttaaa aatcttcttt
    10561 atgaatatcc aatgttactg taatcctgct ccattaaatg cagcattgtt gtcaggtgct
    10621 gcctcttgct tgggaacagc attgggcttt taaatgtctg cagaatctct gcgttcgaag
    10681 ggaattgaga atgaacttcc tggtactgta atgaaaataa ggtctgctca acacagtaaa
    10741 cgtttcctct cttctttaaa a
//
