LOCUS       NM_015323               4226 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens UFM1 specific ligase 1 (UFL1), mRNA.
ACCESSION   NM_015323
VERSION     NM_015323.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   He,C., Xing,X., Chen,H.Y., Gao,M., Shi,J., Xiang,B., Xiao,X.,
            Sun,Y., Yu,H., Xu,G., Yao,Y., Xie,Z., Xing,Y., Budiarto,B.R.,
            Chen,S.Y., Gao,Y., Lee,Y.R. and Zhang,J.
  TITLE     UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance
            anti-tumor immunity
  JOURNAL   Mol Cell 84 (6), 1120-1138 (2024)
   PUBMED   38377992
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Makhlouf,L., Peter,J.J., Magnussen,H.M., Thakur,R., Millrine,D.,
            Minshull,T.C., Harrison,G., Varghese,J., Lamoliatte,F.,
            Foglizzo,M., Macartney,T., Calabrese,A.N., Zeqiraj,E. and
            Kulathu,Y.
  TITLE     The UFM1 E3 ligase recognizes and releases 60S ribosomes from ER
            translocons
  JOURNAL   Nature 627 (8003), 437-444 (2024)
   PUBMED   38383789
REFERENCE   3  (bases 1 to 4226)
  AUTHORS   DaRosa,P.A., Penchev,I., Gumbin,S.C., Scavone,F., Wachalska,M.,
            Paulo,J.A., Ordureau,A., Peter,J.J., Kulathu,Y., Harper,J.W.,
            Becker,T., Beckmann,R. and Kopito,R.R.
  TITLE     UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from
            the ER
  JOURNAL   Nature 627 (8003), 445-452 (2024)
   PUBMED   38383785
REFERENCE   4  (bases 1 to 4226)
  AUTHORS   Luo,H., Jiao,Q.B., Shen,C.B., Gong,W.Y., Yuan,J.H., Liu,Y.Y.,
            Chen,Z., Liu,J., Xu,X.L., Cong,Y.S. and Zhang,X.W.
  TITLE     UFMylation of HRD1 regulates endoplasmic reticulum homeostasis
  JOURNAL   FASEB J 37 (11), e23221 (2023)
   PUBMED   37795761
REFERENCE   5  (bases 1 to 4226)
  AUTHORS   Ishimura,R., Ito,S., Mao,G., Komatsu-Hirota,S., Inada,T., Noda,N.N.
            and Komatsu,M.
  TITLE     Mechanistic insights into the roles of the UFM1 E3 ligase complex
            in ufmylation and ribosome-associated protein quality control
  JOURNAL   Sci Adv 9 (33), eadh3635 (2023)
   PUBMED   37595036
REFERENCE   6  (bases 1 to 4226)
  AUTHORS   Kwon,J., Cho,H.J., Han,S.H., No,J.G., Kwon,J.Y. and Kim,H.
  TITLE     A novel LZAP-binding protein, NLBP, inhibits cell invasion
  JOURNAL   J Biol Chem 285 (16), 12232-12240 (2010)
   PUBMED   20164180
REFERENCE   7  (bases 1 to 4226)
  AUTHORS   Tatsumi,K., Sou,Y.S., Tada,N., Nakamura,E., Iemura,S., Natsume,T.,
            Kang,S.H., Chung,C.H., Kasahara,M., Kominami,E., Yamamoto,M.,
            Tanaka,K. and Komatsu,M.
  TITLE     A novel type of E3 ligase for the Ufm1 conjugation system
  JOURNAL   J Biol Chem 285 (8), 5417-5427 (2010)
   PUBMED   20018847
REFERENCE   8  (bases 1 to 4226)
  AUTHORS   Foster,L.J., Zeemann,P.A., Li,C., Mann,M., Jensen,O.N. and
            Kassem,M.
  TITLE     Differential expression profiling of membrane proteins by
            quantitative proteomics in a human mesenchymal stem cell line
            undergoing osteoblast differentiation
  JOURNAL   Stem Cells 23 (9), 1367-1377 (2005)
   PUBMED   16210410
REFERENCE   9  (bases 1 to 4226)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   10 (bases 1 to 4226)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL590404.5, BP337693.1, AB018319.1 and BC028608.1.
            
            On Nov 23, 2018 this sequence version replaced NM_015323.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036379.2, SRR1660803.203761.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369278.5/ ENSP00000358283.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                AL590404.5         91255-91274
            21-51               BP337693.1         1-31
            52-3786             AB018319.1         1-3735
            3787-4226           BC028608.1         3758-4197
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q16.1"
     gene            1..4226
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /note="UFM1 specific ligase 1"
                     /db_xref="GeneID:23376"
                     /db_xref="HGNC:HGNC:23039"
                     /db_xref="MIM:613372"
     exon            1..145
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..2453
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /note="novel LZAP-binding protein; Regulator of CDK5RAP3
                     and DDRGK1; regulator of C53/LZAP and DDRGK1; E3
                     UFM1-protein transferase 1; multiple alpha-helix protein
                     located at ER"
                     /codon_start=1
                     /product="E3 UFM1-protein ligase 1"
                     /protein_id="NP_056138.1"
                     /db_xref="CCDS:CCDS5034.1"
                     /db_xref="GeneID:23376"
                     /db_xref="HGNC:HGNC:23039"
                     /db_xref="MIM:613372"
                     /translation="MADAWEEIRRLAADFQRAQFAEATQRLSERNCIEIVNKLIAQKQ
                     LEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLIHIENRIGDIIK
                     SEKHVQLVLGQLIDENYLDRLAEEVNDKLQESGQVTISELCKTYDLPGNFLTQALTQR
                     LGRIISGHIDLDNRGVIFTEAFVARHKARIRGLFSAITRPTAVNSLISKYGFQEQLLY
                     SVLEELVNSGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLG
                     IPDAVSYIKKRYKTTQLLFLKAACVGQGLVDQVEASVEEAISSGTWVDIAPLLPTSLS
                     VEDAAILLQQVMRAFSKQASTVVFSDTVVVSEKFINDCTELFRELMHQKAEKEMKNNP
                     VHLITEEDLKQISTLESVSTSKKDKKDERRRKATEGSGSMRGGGGGNAREYKIKKVKK
                     KGRKDDDSDDESQSSHTGKKKPEISFMFQDEIEDFLRKHIQDAPEEFISELAEYLIKP
                     LNKTYLEVVRSVFMSSTTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKFFADDTQ
                     AALTKHLLKSVCTDITNLIFNFLASDLMMAVDDPAAITSEIRKKILSKLSEETKVALT
                     KLHNSLNEKSIEDFISCLDSAAEACDIMVKRGDKKRERQILFQHRQALAEQLKVTEDP
                     ALILHLTSVLLFQFSTHSMLHAPGRCVPQIIAFLNSKIPEDQHALLVKYQGLVVKQLV
                     SQSKKTGQGDYPLNNELDKEQEDVASTTRKELQELSSSIKDLVLKSRKSSVTEE"
     exon            146..291
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            292..320
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            321..418
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            419..533
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            534..664
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            665..723
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            724..870
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            871..1046
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1226
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1347
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1470
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1588
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1755
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1886
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1887..1967
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..2053
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            2054..2234
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..4226
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4203..4208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /note="hexamer: AATAAA"
     polyA_site      4226
                     /gene="UFL1"
                     /gene_synonym="KIAA0776; Maxer; NLBP; RCAD"
                     /note="major polyA site"
ORIGIN      
        1 ctcttctccc accgcctgtc ggctgacgtg tctgcagttc ctccgcgtct actgcgagtc
       61 aggccgtgat ggcggacgcc tgggaagaga ttaggcggtt ggcggccgac ttccagcggg
      121 cgcagttcgc cgaggccacg cagaggttgt ccgagcggaa ctgcattgag attgttaata
      181 aattgattgc tcagaaacag ctagaagtag ttcatacact cgatggaaag gaatatatta
      241 ctccagccca aattagtaaa gaaatgagag atgagctaca tgtccgaggt ggtcgagtaa
      301 acattgttga tctacaacag gtaattaatg tggacctgat tcatattgaa aatagaattg
      361 gtgacattat taaatcagaa aagcatgttc agttagtgtt gggacaactg atagatgaga
      421 attatttgga tcggttggca gaagaggtca atgataaatt gcaagaaagt ggtcaggtca
      481 ccatatcaga actgtgtaaa acttatgatc ttcctgggaa ctttctgaca caggcactaa
      541 ctcagcgact tggtagaatt atcagtggac atattgatct tgataataga ggagtaattt
      601 ttacggaagc ttttgtagct cgacataaag cacgtatccg tggactattc agtgctatta
      661 cccggcctac agctgtgaat tctttgattt caaaatatgg atttcaggag cagcttcttt
      721 actctgtgct tgaggaactt gttaatagcg gacgcttacg aggcactgtg gttggtggga
      781 gacaggataa agctgtgttt gtccctgaca tctactccag gacacagagt acttgggtgg
      841 attccttttt caggcagaat ggctatctag aatttgatgc tttgtccaga cttggaatcc
      901 cagatgctgt aagctacata aagaaaagat ataagactac acaactcttg tttttgaaag
      961 cagcttgtgt tggtcaagga cttgtggatc aagtggaagc atcagtagaa gaagccatca
     1021 gctctggaac atgggttgat attgcacctc tgctacccac ttctttatca gttgaagatg
     1081 ctgccatatt gcttcagcag gtgatgaggg cattcagcaa acaggcctca actgtagtct
     1141 ttagcgacac tgttgtagtc agtgaaaaat ttataaatga ctgtacagaa ctgttccgtg
     1201 agctgatgca ccagaaagct gaaaaggaaa tgaaaaataa tcctgtgcat ttaatcactg
     1261 aagaagatct gaaacaaatc tccactttag aaagcgttag tacaagtaaa aaggataaaa
     1321 aagatgagcg aagaaggaaa gcaacagagg gcagtggaag catgagagga ggaggtgggg
     1381 gcaatgccag agagtacaaa attaaaaaag tcaagaagaa aggaagaaaa gatgatgata
     1441 gtgatgatga atctcaatca tcccacactg gaaagaagaa gccagagatc agttttatgt
     1501 tccaggatga gattgaagat tttttaagaa aacacataca agatgcccct gaggagttta
     1561 tttcggaact tgctgagtac ttaataaaac ctcttaataa aacttatctc gaggtggtac
     1621 gttcagtatt catgtcttca acaacttctg cttctgggac gggcagaaaa cgcacaatca
     1681 aggacttgca agaagaagtt tcaaacctgt acaataacat taggttattt gaaaaaggga
     1741 tgaagttttt tgcagatgac acacaggctg ctcttaccaa acacttgctg aagtcagtgt
     1801 gtactgatat cactaacctc attttcaact tcttagcttc ggatttaatg atggcagtag
     1861 acgatcctgc agccattaca agtgaaataa gaaagaaaat tttaagtaaa ttatcagaag
     1921 aaaccaaagt agctcttaca aaactccata actctctgaa tgaaaagagc atagaagact
     1981 ttatttcttg tctggattct gcagcagaag cttgtgatat tatggtgaaa aggggagaca
     2041 aaaaaaggga aagacagata ctgttccaac atcgacaagc actggctgaa cagctaaagg
     2101 tcacagaaga ccctgctctt attctgcacc tcacatcagt cctgttgttt cagttttcaa
     2161 cccacagcat gctccatgca cctggaagat gtgtcccaca gatcattgct tttcttaata
     2221 gtaaaattcc agaggatcag catgctcttt tggtaaagta tcaaggtttg gttgtaaagc
     2281 agctagtcag tcaaagtaag aagactgggc agggagatta tcccttgaat aatgaattag
     2341 acaaagaaca agaagatgtt gccagtacta ctcgtaaaga gcttcaagaa ctttcttcat
     2401 ccattaaaga ccttgttctc aaatctagga aatcatctgt gacggaagag taatgatctt
     2461 aatttacatt tgtcatatag taagcatttt cccccaaggt tgaaggtgag tggtcacaaa
     2521 aaagtagtca ctatacaact cccctctccc tgcaaaaacc acctcataca cacacaattc
     2581 agttaaaaca gtagtattgt attaaatgta aatcttaaaa agatgtgaat ttttgtaaat
     2641 tgggttcttc atggaagttt ttttccacct gattttcaca caaatactat atgaaatttt
     2701 tcacattatt ttcacataat tttaaaaatt acatatttca ggtttgttct ctttccaaat
     2761 gttgaatgaa aaacaaattt tccaatccat ttatccctgg ggaaggattc atttgttgcg
     2821 agtgccagta tacttaaatg tatactttat atacatttaa gcagaaattt aaaatgtggt
     2881 cttttttttt taaaagaaac acttctgtgt cagaaatgaa aataaatcat gttttttatt
     2941 tttaaataga aagcacttca aaactaaaat acagaaattt acaaaaaaga aaaattatta
     3001 ctaagccctt gctcttattt ttggcagaat aaacattgat tttggttggg gctgcaagga
     3061 tgcctttaca ggtgcacaag aacgctagag tggcccatgc attgctgtgt tctcttctaa
     3121 aacaagctac taacaagaga gagaacacat gcaatttatt accagtaagc ataagtcata
     3181 tttaacattg gaataactaa gcattttata caaagatttt tctttgaccc agtttgcatt
     3241 tgtacatttt atttttttag ttcaacagct gctctgtcac ataatacttt gtaaatacta
     3301 gtgtgaaaaa cagataggat gccttttcca gtggtaggag catctgtaag tgcatttaaa
     3361 tcattagaag cttttttcca aaactagaaa cccttattaa aaaatagaca tttttttcta
     3421 aagattatta gttaaaaata actaaaagag ttccttagag taatcatttc aaccaaatga
     3481 atttcaagca acaacttact tactatttat caaaaaggga tttaattatt ttgaatataa
     3541 ttataaatta aatttcaaat atttaaaaat actattttat ataattcctt atgacatttt
     3601 aaactttata ttttaaaata tttgaatacg aaaagatcag tctacctcta ctccattcta
     3661 gacaaagtat taatccttag tgaaagtaat taattaaatt gccaacttgg cattattatt
     3721 aaacttgata ggaacgcaac attaaattta aaaatgtttt cccgtgggta attttctatt
     3781 atatattttc atatgggcaa agggaaaaaa tgataaatcc tctgtaatca caaaccccaa
     3841 tttcgttttg tttattcagc ttctaaaata ttgaacaccc agacttttaa ttcaaccttt
     3901 aagaacctta tcatttatgt ttcagtagat atcaaagtaa tccatgtttg tgtcaaatga
     3961 tcatagaaaa taaatagaag agacagtgaa gcaagtaaaa agaaaagcat tgttttaatt
     4021 tgtttgcatt aatttttttc atttgtcaaa atgcttcttt tgttgccaca gtaaagaaca
     4081 gtttttattg ttttgtaagt aaaattacgt agctgatttt gtatgtaaag attaatttcc
     4141 ataataaaaa ttattgtatg tttactgtga tcttaatggg cagggttaag aaagttattt
     4201 aaaataaagt tacctattct actaaa
//
