LOCUS       NP_060329                548 aa            linear   PRI 28-APR-2025
DEFINITION  uridine-cytidine kinase-like 1 isoform 1 [Homo sapiens].
ACCESSION   NP_060329
VERSION     NP_060329.2
DBSOURCE    REFSEQ: accession NM_017859.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 548)
  AUTHORS   Matchett,E.C., Ambrose,E.C. and Kornbluth,J.
  TITLE     Characterization of uridine-cytidine kinase like-1 nucleoside
            kinase activity and its role in tumor growth
  JOURNAL   Biochem J 479 (11), 1149-1164 (2022)
   PUBMED   35583288
  REMARK    GeneRIF: Characterization of uridine-cytidine kinase like-1
            nucleoside kinase activity and its role in tumor growth.
REFERENCE   2  (residues 1 to 548)
  AUTHORS   Buivydiene,A., Liakina,V., Kashuba,E., Norkuniene,J.,
            Jokubauskiene,S., Gineikiene,E. and Valantinas,J.
  TITLE     Impact of the UridineCytidine Kinase Like-1 Protein and IL28B
            rs12979860 and rs8099917 SNPs on the Development of Hepatocellular
            Carcinoma in Cirrhotic Chronic Hepatitis C Patients-A Pilot Study
  JOURNAL   Medicina (Kaunas) 54 (5), 67 (2018)
   PUBMED   30344298
  REMARK    GeneRIF: The presence of IL28B rs8099917 TT and rs12979860 CC SNPs,
            but not the intensity of UCKL-1 expression, is strongly associated
            with increased chances of Hepatocellular Carcinoma development in
            hepatitis C virus -positive cirrhotic patients.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 548)
  AUTHORS   Ambrose,E.C. and Kornbluth,J.
  TITLE     Downregulation of uridine-cytidine kinase like-1 decreases
            proliferation and enhances tumor susceptibility to lysis by
            apoptotic agents and natural killer cells
  JOURNAL   Apoptosis 14 (10), 1227-1236 (2009)
   PUBMED   19653100
  REMARK    GeneRIF: These results indicate a role for UCKL-1 in tumor cell
            survival.
REFERENCE   4  (residues 1 to 548)
  AUTHORS   Fortier,J.M. and Kornbluth,J.
  TITLE     NK lytic-associated molecule, involved in NK cytotoxic function, is
            an E3 ligase
  JOURNAL   J Immunol 176 (11), 6454-6463 (2006)
   PUBMED   16709802
REFERENCE   5  (residues 1 to 548)
  AUTHORS   Kashuba,E., Kashuba,V., Sandalova,T., Klein,G. and Szekely,L.
  TITLE     Epstein-Barr virus encoded nuclear protein EBNA-3 binds a novel
            human uridine kinase/uracil phosphoribosyltransferase
  JOURNAL   BMC Cell Biol 3, 23 (2002)
   PUBMED   12199906
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB193745.1, AK000524.1 and
            AK222559.1.
            
            On Jan 15, 2005 this sequence version replaced NP_060329.1.
            
            Summary: The protein encoded by this gene is a uridine kinase.
            Uridine kinases catalyze the phosphorylation of uridine to uridine
            monophosphate. This protein has been shown to bind to Epstein-Barr
            nuclear antigen 3 as well as natural killer lytic-associated
            molecule. Ubiquitination of this protein is enhanced by the
            presence of natural killer lytic-associated molecule. In addition,
            protein levels decrease in the presence of natural killer
            lytic-associated molecule, suggesting that association with natural
            killer lytic-associated molecule results in ubiquitination and
            subsequent degradation of this protein. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Nov
            2014].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK222559.1, AK000524.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354216.11/ ENSP00000346155.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.33"
     Protein         1..548
                     /product="uridine-cytidine kinase-like 1 isoform 1"
                     /EC_number="2.7.1.48"
                     /note="uridine-cytidine kinase-like 1; UCK1-LIKE"
                     /calculated_mol_wt=61010
     Region          1..78
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9NWZ5.2)"
     Site            16
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:16964243,
                     ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163;
                     propagated from UniProtKB/Swiss-Prot (Q9NWZ5.2)"
     Site            56
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q9NWZ5.2)"
     Site            63
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q91YL3; propagated from
                     UniProtKB/Swiss-Prot (Q9NWZ5.2)"
     Region          101..299
                     /region_name="UMPK"
                     /note="Uridine monophosphate kinase (UMPK, EC 2.7.1.48),
                     also known as uridine kinase or uridine-cytidine kinase
                     (UCK), catalyzes the reversible phosphoryl transfer from
                     ATP to uridine or cytidine to yield UMP or CMP. In the
                     primidine nucleotide-salvage pathway; cd02023"
                     /db_xref="CDD:238981"
     Site            order(112,236,284)
                     /site_type="other"
                     /note="ATP-binding site [chemical binding]"
                     /db_xref="CDD:238981"
     Site            order(133,157,237)
                     /site_type="active"
                     /note="Sugar specificity [active]"
                     /db_xref="CDD:238981"
     Site            order(136,154,183,185,188,245)
                     /site_type="active"
                     /note="Pyrimidine base specificity [active]"
                     /db_xref="CDD:238981"
     Region          329..532
                     /region_name="UPRTase"
                     /note="Uracil phosphoribosyltransferase; pfam14681"
                     /db_xref="CDD:434124"
     Site            order(400,402,453..455,457..461,487)
                     /site_type="active"
                     /db_xref="CDD:206754"
     Site            539
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:20068231,
                     ECO:0007744|PubMed:21406692; propagated from
                     UniProtKB/Swiss-Prot (Q9NWZ5.2)"
     CDS             1..548
                     /gene="UCKL1"
                     /gene_synonym="UCK1L; URKL1"
                     /coded_by="NM_017859.4:45..1691"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS13547.1"
                     /db_xref="GeneID:54963"
                     /db_xref="HGNC:HGNC:15938"
                     /db_xref="MIM:610866"
ORIGIN      
        1 maapparada dpsptsppta rdtpgrqaek setacedrsn aesldrllpp vgtgrsprkr
       61 ttsqcksepp llrtskrtiy tagrppwyne hgtqskeafa iglgggsasg kttvarmiie
      121 aldvpwvvll smdsfykvlt eqqqeqaahn nfnfdhpdaf dfdliistlk klkqgksvkv
      181 piydftthsr kkdwktlyga nviifegima fadktllell dmkifvdtds dirlvrrlrr
      241 disergrdie gvikqynkfv kpsfdqyiqp tmrladivvp rgsgntvaid livqhvhsql
      301 eerelsvraa lasahqchpl prtlsvlkst pqvrgmhtii rdketsrdef ifyskrlmrl
      361 liehalsflp fqdcvvqtpq gqdyagkcya gkqitgvsil ragetmepal ravckdvrig
      421 tiliqtnqlt gepelhylrl pkdisddhvi lmdctvstga aammavrvll dhdvpedkif
      481 llsllmaemg vhsvayafpr vriittavdk rvndlfriip gignfgdryf gtdavpdgsd
      541 eeevaytg
//
