LOCUS       NM_012474               4801 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens uridine-cytidine kinase 2 (UCK2), transcript variant
            1, mRNA.
ACCESSION   NM_012474
VERSION     NM_012474.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Malami,I., Alhassan,A.M., Adamu,A.A., Bello,M.B., Muhammad,A. and
            Imam,M.U.
  TITLE     Cytotoxic Flavokawain B Inhibits the Growth and Metastasis of
            Hepatocellular Carcinoma through UCK2 Modulation of the
            STAT3/Hif-1alpha/VEGF Signalling Pathway
  JOURNAL   Curr Drug Targets 24 (11), 919-928 (2023)
   PUBMED   37534791
  REMARK    GeneRIF: Cytotoxic Flavokawain B Inhibits the Growth and Metastasis
            of Hepatocellular Carcinoma through UCK2 Modulation of the
            STAT3/Hif-1alpha/VEGF Signalling Pathway.
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Wu,D., Zhang,C., Liao,G., Leng,K., Dong,B., Yu,Y., Tai,H.,
            Huang,L., Luo,F., Zhang,B., Zhan,T., Hu,Q. and Tai,S.
  TITLE     Targeting uridine-cytidine kinase 2 induced cell cycle arrest
            through dual mechanism and could improve the immune response of
            hepatocellular carcinoma
  JOURNAL   Cell Mol Biol Lett 27 (1), 105 (2022)
   PUBMED   36447138
  REMARK    GeneRIF: Targeting uridine-cytidine kinase 2 induced cell cycle
            arrest through dual mechanism and could improve the immune response
            of hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4801)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4801)
  AUTHORS   Wu,Y., Jamal,M., Xie,T., Sun,J., Song,T., Yin,Q., Li,J., Pan,S.,
            Zeng,X., Xie,S. and Zhang,Q.
  TITLE     Uridine-cytidine kinase 2 (UCK2): A potential diagnostic and
            prognostic biomarker for lung cancer
  JOURNAL   Cancer Sci 110 (9), 2734-2747 (2019)
   PUBMED   31278886
  REMARK    GeneRIF: Lung cancer patients with high UCK2 expression had poorer
            first progression survival and overall survival than patients with
            low UCK2 expression. Cytology experiments show that knockdown of
            UCK2 could suppress the proliferation and migration of lung cancer
            cells. Study indicated that UCK2 might be a potential early
            diagnostic and prognostic biomarker for lung cancer.
REFERENCE   5  (bases 1 to 4801)
  AUTHORS   Suzuki,N.N., Koizumi,K., Fukushima,M., Matsuda,A. and Inagaki,F.
  TITLE     Structural basis for the specificity, catalysis, and regulation of
            human uridine-cytidine kinase
  JOURNAL   Structure 12 (5), 751-764 (2004)
   PUBMED   15130468
REFERENCE   6  (bases 1 to 4801)
  AUTHORS   Suzuki,N.N., Koizumi,K., Fukushima,M., Matsuda,A. and Inagaki,F.
  TITLE     Crystallization and preliminary X-ray analysis of human
            uridine-cytidine kinase 2
  JOURNAL   Acta Crystallogr D Biol Crystallogr 59 (Pt 8), 1477-1478 (2003)
   PUBMED   12876357
REFERENCE   7  (bases 1 to 4801)
  AUTHORS   Kashuba,E., Kashuba,V., Sandalova,T., Klein,G. and Szekely,L.
  TITLE     Epstein-Barr virus encoded nuclear protein EBNA-3 binds a novel
            human uridine kinase/uracil phosphoribosyltransferase
  JOURNAL   BMC Cell Biol 3, 23 (2002)
   PUBMED   12199906
  REMARK    GeneRIF: Increased intranuclear levels of UK may contribute to the
            metabolic build-up that is needed for Epstein-Barr virus nuclear
            protein EBNA-3-mediated B-cell blast transformation and rapid
            proliferation.
REFERENCE   8  (bases 1 to 4801)
  AUTHORS   Koizumi,K., Shimamoto,Y., Azuma,A., Wataya,Y., Matsuda,A.,
            Sasaki,T. and Fukushima,M.
  TITLE     Cloning and expression of uridine/cytidine kinase cDNA from human
            fibrosarcoma cells
  JOURNAL   Int J Mol Med 8 (3), 273-278 (2001)
   PUBMED   11494055
REFERENCE   9  (bases 1 to 4801)
  AUTHORS   Van Rompay,A.R., Norda,A., Linden,K., Johansson,M. and Karlsson,A.
  TITLE     Phosphorylation of uridine and cytidine nucleoside analogs by two
            human uridine-cytidine kinases
  JOURNAL   Mol Pharmacol 59 (5), 1181-1186 (2001)
   PUBMED   11306702
REFERENCE   10 (bases 1 to 4801)
  AUTHORS   Ozaki,K., Kuroki,T., Hayashi,S. and Nakamura,Y.
  TITLE     Isolation of three testis-specific genes (TSA303, TSA806, TSA903)
            by a differential mRNA display method
  JOURNAL   Genomics 36 (2), 316-319 (1996)
   PUBMED   8812458
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DR762696.1, BC002906.2,
            AL358115.18 and BM714864.1.
            
            On Nov 22, 2018 this sequence version replaced NM_012474.4.
            
            Summary: This gene encodes a pyrimidine ribonucleoside kinase. The
            encoded protein (EC 2.7.1.48) catalyzes phosphorylation of uridine
            and cytidine to uridine monophosphate (UMP) and cytidine
            monophosphate (CMP), respectively.[provided by RefSeq, Oct 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.159658.1,
                                           SRR1660805.217603.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367879.9/ ENSP00000356853.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                DR762696.1         120-160
            42-1223             BC002906.2         1-1182
            1224-4329           AL358115.18        74751-77856
            4330-4801           BM714864.1         1-472
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q24.1"
     gene            1..4801
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /note="uridine-cytidine kinase 2"
                     /db_xref="GeneID:7371"
                     /db_xref="HGNC:HGNC:12562"
                     /db_xref="MIM:609329"
     exon            1..319
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /inference="alignment:Splign:2.1.0"
     CDS             221..1006
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /EC_number="2.7.1.48"
                     /note="isoform 1 is encoded by transcript variant 1;
                     uridine monophosphate kinase; uridine kinase; uridine
                     monophosphokinase 2; cytidine monophosphokinase 2;
                     testis-specific protein TSA903"
                     /codon_start=1
                     /product="uridine-cytidine kinase 2 isoform 1"
                     /protein_id="NP_036606.2"
                     /db_xref="CCDS:CCDS1252.1"
                     /db_xref="GeneID:7371"
                     /db_xref="HGNC:HGNC:12562"
                     /db_xref="MIM:609329"
                     /translation="MAGDSEQTLQNHQQPNGGEPFLIGVSGGTASGKSSVCAKIVQLL
                     GQNEVDYRQKQVVILSQDSFYRVLTSEQKAKALKGQFNFDHPDAFDNELILKTLKEIT
                     EGKTVQIPVYDFVSHSRKEETVTVYPADVVLFEGILAFYSQEVRDLFQMKLFVDTDAD
                     TRLSRRVLRDISERGRDLEQILSQYITFVKPAFEEFCLPTKKYADVIIPRGADNLVAI
                     NLIVQHIQDILNGGPSKRQTNGCLNGYTPSRKRQASESSSRPH"
     exon            320..479
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /inference="alignment:Splign:2.1.0"
     exon            480..576
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /inference="alignment:Splign:2.1.0"
     exon            577..719
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /inference="alignment:Splign:2.1.0"
     exon            720..817
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /inference="alignment:Splign:2.1.0"
     exon            818..866
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /inference="alignment:Splign:2.1.0"
     exon            867..4801
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1269..1274
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /note="hexamer: AATACA"
     polyA_site      1292
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /note="major polyA site"
     regulatory      4781..4786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
                     /note="hexamer: AATAAA"
     polyA_site      4801
                     /gene="UCK2"
                     /gene_synonym="TSA903; UK; UMPK"
ORIGIN      
        1 gtaaaccgga ccagccgctg cgggcaaagg aaggctcttg gctccttcgg gaaacccagc
       61 cccgtcaccg ggctccgagc ggctcgcagg cgagcgacag cggcctcagc cccggcagcg
      121 cccagcggcg gctgcggaaa gcggagggag tccgacgcgg gcgcgggcgg ggagcgtgcg
      181 tccgttcgca caggcagcgg gaggaggggc ggcgcgaacc atggccgggg acagcgagca
      241 gaccctgcag aaccaccagc agcccaacgg cggcgagccc ttccttatag gcgtcagcgg
      301 gggaacagct agcggcaagt cttccgtgtg tgctaagatc gtgcagctcc tggggcagaa
      361 tgaggtggac tatcgccaga agcaggtggt catcctgagc caggatagct tctaccgtgt
      421 ccttacctcg gagcagaagg ccaaagccct gaagggccag ttcaactttg accacccgga
      481 tgcctttgac aatgaactca ttctcaaaac actcaaagaa atcactgaag ggaaaacagt
      541 ccagatcccc gtgtatgact ttgtctccca ttcccggaag gaggagacag ttactgtcta
      601 tcccgcagac gtggtgctct ttgaagggat cctggccttc tactcccagg aggtacgaga
      661 cctgttccag atgaagcttt ttgtggatac agatgcggac acccggctct cacgcagagt
      721 attaagggac atcagcgaga gaggcaggga tcttgagcag attttatctc agtacattac
      781 gttcgtcaag cctgcctttg aggaattctg cttgccaaca aagaagtatg ctgatgtgat
      841 catccctaga ggtgcagata atctggtggc catcaacctc atcgtgcagc acatccagga
      901 catcctgaat ggagggccct ccaaacggca gaccaatggc tgtctcaacg gctacacccc
      961 ttcacgcaag aggcaggcat cggagtccag cagcaggccg cattgacccg tctccatcgg
     1021 accccagccc ctatctccaa gagacagagg aggggtcagg aggcactgct catctgtaca
     1081 tactgtttcc tatgacatta ctgtatttaa gaaaacacca tggagatgaa atgcctttga
     1141 tttttttttt ctttttgtac tttggaacga caaaatgaaa cagaacttga ccctgagctt
     1201 aaataacaaa actgtgccaa ctactactgg tgatgcctaa ttatgaatcc aacgtgtaac
     1261 cagttataaa tacatatata tataaaaaag gatctatttt tgtgtaaatg tacaaatact
     1321 gtaaagctgt ttgccataag tattttgcag gagggtctgt acttgagtgt tttaggactg
     1381 gagctggaat ttcaccaagg ggaaattgcc tgttgtgtcc cagggtgggg aggctcgggc
     1441 atctgtgaga gacagggagg gtggctaagg tccagttgct tttgagtgcc ccgtggaact
     1501 tgatgggaat ggggttggaa aaccttgatt gcctcctctg tcacatgcca ctcttggagc
     1561 tggagcttag tgatatgtca atgagagata ctcttgtctt tttttttttt cttttctttt
     1621 ttttttttga gttgaaatta ggtaaaatct caactcaaaa atcaaactca gggaagaggc
     1681 ccactgagga gaacaagtct tctgcatttt cttcctgccc tgctcccacc ttctcgcctc
     1741 tggctgcttg gatagcttgg tgactccttc ctcaccctcc tgcttctctg tctctccaag
     1801 aagccagagc tgcctctttc cttccctgga agagatttta tgggatcgct tcctgataga
     1861 agatgggaag gagagggcag aactttgcct gctgtcatat ctgaagaatc actggttctt
     1921 tctgcatccc aggggcgtaa ctgatctgac tccttgggct ttttcctgta ttgtggaata
     1981 tagagtggac cattgcaggc tgggtgtggg tgagggcttc ggtaactgat gccggcagca
     2041 gcgcctcatg gagtggcagg ggagctctgt ctgtggaggg gtagtagttt ccgcttcctg
     2101 gaggtttgca gtgaggtgag gagagtaact ttggtcaggg attctcacta gcagcgtcat
     2161 ggccaggatg ctttacttat ttgcaatacc acctagtgga tatggcaact gcagagcata
     2221 aaaggggctc ggaaaagcag tttttacaaa gtatttaaaa ggagagtatt taaatcatag
     2281 gtacctaagt tggagcctga ggttgaaata tttggggtgg agatttctag ctttcctgag
     2341 agctgctgct tggtggtagt gttttctctg gaagtacaga ttcctgacat tctgggtgga
     2401 ggggagccca agtgagcctg aaggagttct gattatagag tcccgagcct cagtttctga
     2461 ctgcagaaga gccagagatg ttcatttcat tgttgaccac cctagtgctt ggtggactgt
     2521 gcccttagct atcccactgt ggctgccatt gccagaaaca ttttggagaa ccttttcttc
     2581 ccattgctct cagagcctgt tcgagccatt caggtaaatt ggtctccatt attacctggt
     2641 cacaccagat tttcccccag actcagcacc atccagtctg attccccgtt tctgggctca
     2701 gtttgactct aaatctgttt tgctatttta aaaagtcaaa cctggtcccc agcagagctt
     2761 gccacccgga gactttcagg agaccgtgca ggctctgaag ggagctccca gtcacagcag
     2821 ccgcctactt ggagcaaggg aaggctccca agatgcctcc tttgaaagcc ggtgctcacc
     2881 aagatcagtt caggatcaat cattatcctt gtagtcatgc cttgatcagt tctgagatga
     2941 agcagggaat gcaggcctgg gttagtatcc tgaggacaca gggcccctgg ccttgatggc
     3001 actgggctgt gctcaggtga agcccacccg tgtctgggag agccttggct tttcagtggg
     3061 cagcatgctc aactttactt gaagctccca ggtgccaaac catttagtgc cttggctgtt
     3121 tatcccactg ccttggaaga tggactcctc tcatctctga gcctttacct agctgcttcc
     3181 tcatctacct gtggtgttgg gaccaagcct ccaatatctc tgaatagccc actcctagct
     3241 tggaaacagc tataagggct gccctgcccc ttgtcaaatg atcaccaatt cttggtggct
     3301 tggctgggga caatgaacag tgtctgctgg ggtctgtgac acactgtttc tggaacaagg
     3361 aagggatgga aggctctacc cctccgtggc agcccagcat ttggccaact ctgtatctga
     3421 acttgagctt tctatggaac aaacaatggt cttagttttt taagagcctc tgggtctctg
     3481 attccatgga gagtcaggag tagaattagt ggaccagaaa ccatgacatt tgccatctag
     3541 gtactctgct gttcagggtg gaggcacaga tgagcagagt ggggtgatgt tggttgtatg
     3601 tgcatagaag tcccctggct ccagagccat ccagtggtgc ttggttctct cttcctgccc
     3661 aggtattgtg tgtggaccaa gtgtttagga aacaagcata gaagcattat gtcacaggaa
     3721 ggcctggcct ctcactcctt cacactaatg agccttctac tgacccaagt ttctttttct
     3781 cgggcagcct gggaccacta tgagcagaga gatgggagtc tgtgtaagaa ggatgcaagt
     3841 atcctttgat ttggagtgga cagagcaggt ccttgcaaag gcagtgggtt aaacaacggg
     3901 atgtctgtgg gacaggccag ggaaggaggg cagagacagg gtctccagtt caccttgccg
     3961 cagaactagc ctaatcatcc ctgcttctgg tcttggccac ttggcaatca tggggaagtt
     4021 gactccccgc ctcacttggg cttggagctg gatacttgtg gccacgccca ggataggttg
     4081 ccagagtttc attaggccgc tgggtcgggc tggaggcttg tgtacacctc ggagcagtcg
     4141 ggcagcagga gggccggaag cctggttggt ttgtatatga cctctcttgc ccttgtgcac
     4201 tttccttgat ttagcaacct tgacctgcac tggccctcac agctgttgga gggaagtagc
     4261 ctcttgcagg gaaatatttt cccctgccct ggaatctcct atgtgcctgt taaagattcc
     4321 agggccaaga actcggcagg ctgaggagca gctaggtagg tgggttatgc ggttcaggac
     4381 tgcttctgga agggactgcc tgtacttctg taccaccgtt gccctttaca ctttgctcag
     4441 ggcggggtgg gggaagcatt caaacaaaac aaggaaggga actgtctggc aaagcataag
     4501 tggatgcatc cagagctcag tcccctttaa tcttttgtct ctgggcgttc tgctgccttc
     4561 ctcataccgg ggactggcat tccaggtcag cttggctgtg gtcttagagg cagggagtgc
     4621 ctacccagtc ctgcctcagg agcagggtga gtagctaaat acagacttag gctttttttt
     4681 cccccctttt aagatgcttg ctcctctccc ttttcttttt accaccctac ctttattgtt
     4741 agtggttaca aagtgaccac atattatgta ctttgctgta aataaagaca gacaaaaagg
     4801 c
//
