LOCUS       NM_031432               2162 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens uridine-cytidine kinase 1 (UCK1), transcript variant
            1, mRNA.
ACCESSION   NM_031432
VERSION     NM_031432.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Meinsma,R. and van Kuilenburg,A.B.
  TITLE     Purification, activity, and expression levels of two
            uridine-cytidine kinase isoforms in neuroblastoma cell lines
  JOURNAL   Nucleosides Nucleotides Nucleic Acids 35 (10-12), 613-618 (2016)
   PUBMED   27906629
  REMARK    GeneRIF: UCK1 and 2 are both expressed in several neuroblastoma
            cell lines, including four MYCN single copy cell lines and five
            MYCN amplified cell lines
REFERENCE   3  (bases 1 to 2162)
  AUTHORS   van Kuilenburg,A.B. and Meinsma,R.
  TITLE     The pivotal role of uridine-cytidine kinases in pyrimidine
            metabolism and activation of cytotoxic nucleoside analogues in
            neuroblastoma
  JOURNAL   Biochim Biophys Acta 1862 (9), 1504-1512 (2016)
   PUBMED   27239701
  REMARK    GeneRIF: Knockdown of endogenous UCK2 as well as overexpression of
            UCK1 resulted in decreased metabolism of uridine and cytidine and
            protected neuroblastoma cells from 3-deazauridine-induced toxicity.
            Subcellular localization studies showed that UCK1-GFP and UCK2-GFP
            were localized in the cell nucleus and cytosol, respectively.
REFERENCE   4  (bases 1 to 2162)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   5  (bases 1 to 2162)
  AUTHORS   Hasegawa,Y., Taylor,D., Ovchinnikov,D.A., Wolvetang,E.J., de
            Torrente,L. and Mar,J.C.
  TITLE     Variability of Gene Expression Identifies Transcriptional
            Regulators of Early Human Embryonic Development
  JOURNAL   PLoS Genet 11 (8), e1005428 (2015)
   PUBMED   26288249
  REMARK    GeneRIF: Results from a study on gene expression variability
            markers in early-stage human embryos shows that UCK1 is a putative
            expression variability marker for the 3-day, 8-cell embryo stage.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2162)
  AUTHORS   Valencia,A., Masala,E., Rossi,A., Martino,A., Sanna,A., Buchi,F.,
            Canzian,F., Cilloni,D., Gaidano,V., Voso,M.T., Kosmider,O.,
            Fontenay,M., Gozzini,A., Bosi,A. and Santini,V.
  TITLE     Expression of nucleoside-metabolizing enzymes in myelodysplastic
            syndromes and modulation of response to azacitidine
  JOURNAL   Leukemia 28 (3), 621-628 (2014)
   PUBMED   24192812
REFERENCE   7  (bases 1 to 2162)
  AUTHORS   Golitsina,N.L., Danehy,F.T. Jr., Fellows,R., Cretton-Scott,E. and
            Standring,D.N.
  TITLE     Evaluation of the role of three candidate human kinases in the
            conversion of the hepatitis C virus inhibitor 2'-C-methyl-cytidine
            to its 5'-monophosphate metabolite
  JOURNAL   Antiviral Res 85 (3), 470-481 (2010)
   PUBMED   19883694
REFERENCE   8  (bases 1 to 2162)
  AUTHORS   Virovic Jukic,L., Duvnjak,M., Wu,C.H. and Wu,G.Y.
  TITLE     Human uridine-cytidine kinase phosphorylation of ribavirin: a
            convenient method for activation of ribavirin for conjugation to
            proteins
  JOURNAL   J Biomed Sci 15 (2), 205-213 (2008)
   PUBMED   18080217
  REMARK    GeneRIF: ribavirin is a potential substrate of UCK-1, and RMP
            formed could be chemically coupled to AsOR to form a conjugate for
            liver specific targeting.
REFERENCE   9  (bases 1 to 2162)
  AUTHORS   Van Rompay,A.R., Norda,A., Linden,K., Johansson,M. and Karlsson,A.
  TITLE     Phosphorylation of uridine and cytidine nucleoside analogs by two
            human uridine-cytidine kinases
  JOURNAL   Mol Pharmacol 59 (5), 1181-1186 (2001)
   PUBMED   11306702
REFERENCE   10 (bases 1 to 2162)
  AUTHORS   Greenberg,N., Schumm,D.E. and Webb,T.E.
  TITLE     Uridine kinase activities and pyrimidine nucleoside phosphorylation
            in fluoropyrimidine-sensitive and -resistant cell lines of the
            Novikoff hepatoma
  JOURNAL   Biochem J 164 (2), 379-387 (1977)
   PUBMED   195585
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL358781.19.
            
            On Aug 31, 2019 this sequence version replaced NM_031432.4.
            
            Summary: This gene encodes a uridine-cytidine kinase that catalyzes
            the phosphorylation of uridine and cytidine to uridine
            monophosphate (UMP) and cytidine monophosphate (CMP) but not the
            phosphorylation of deoxyribonucleosides or purine ribonucleosides.
            This enzyme can also phosphorylate uridine and cytidine analogs and
            uses both ATP and GTP as a phosphate donor. Alternative splicing
            results in multiple splice variants encoding distinct isoforms.
            [provided by RefSeq, May 2012].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes the isoform a.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC091495.1, AK022317.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372215.5/ ENSP00000361289.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-197               AL358781.19        135333-135529       c
            198-357             AL358781.19        134752-134911       c
            358-454             AL358781.19        133754-133850       c
            455-597             AL358781.19        133394-133536       c
            598-692             AL358781.19        133210-133304       c
            693-741             AL358781.19        130195-130243       c
            742-2162            AL358781.19        128067-129487       c
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.13"
     gene            1..2162
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /note="uridine-cytidine kinase 1"
                     /db_xref="GeneID:83549"
                     /db_xref="HGNC:HGNC:14859"
                     /db_xref="MIM:609328"
     exon            1..197
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..923
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /EC_number="2.7.1.48"
                     /note="isoform a is encoded by transcript variant 1;
                     uridine monophosphokinase 1; cytidine monophosphokinase 1"
                     /codon_start=1
                     /product="uridine-cytidine kinase 1 isoform a"
                     /protein_id="NP_113620.1"
                     /db_xref="CCDS:CCDS6944.1"
                     /db_xref="GeneID:83549"
                     /db_xref="HGNC:HGNC:14859"
                     /db_xref="MIM:609328"
                     /translation="MASAGGEDCESPAPEADRPHQRPFLIGVSGGTASGKSTVCEKIM
                     ELLGQNEVEQRQRKVVILSQDRFYKVLTAEQKAKALKGQYNFDHPDAFDNDLMHRTLK
                     NIVEGKTVEVPTYDFVTHSRLPETTVVYPADVVLFEGILVFYSQEIRDMFHLRLFVDT
                     DSDVRLSRRVLRDVRRGRDLEQILTQYTTFVKPAFEEFCLPTKKYADVIIPRGVDNMV
                     AINLIVQHIQDILNGDICKWHRGGSNGRSYKRTFSEPGDHPGMLTSGKRSHLESSSRP
                     H"
     exon            198..357
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /inference="alignment:Splign:2.1.0"
     exon            358..454
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /inference="alignment:Splign:2.1.0"
     exon            455..597
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /inference="alignment:Splign:2.1.0"
     exon            598..692
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /inference="alignment:Splign:2.1.0"
     exon            693..741
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /inference="alignment:Splign:2.1.0"
     exon            742..2162
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2137..2142
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /note="hexamer: AATAAA"
     regulatory      2144..2149
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /note="hexamer: AACAAA"
     polyA_site      2158
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /note="major polyA site"
     polyA_site      2162
                     /gene="UCK1"
                     /gene_synonym="URK1"
                     /note="major polyA site"
ORIGIN      
        1 ggttgcctcc gacctcggcg ctgggcgggc gcgccgggcc tggggaaggg gcgggcgcgg
       61 ggacccgatg cgcgggagcg gaggccgaga tggcttcggc gggaggcgaa gactgcgaga
      121 gccccgcgcc ggaggccgac cgtccgcacc agcggccctt cctgataggg gtgagcggcg
      181 gcactgccag cgggaagtcg accgtgtgtg agaagatcat ggagttgctg ggacagaacg
      241 aggtggaaca gcggcagcgg aaggtggtca tcctgagcca ggacaggttc tacaaggtcc
      301 tgacggcaga gcagaaggcc aaggccttga aaggacagta caattttgac catccagatg
      361 cctttgataa tgatttgatg cacaggactc tgaagaacat cgtggagggc aaaacggtgg
      421 aggtgccgac ctatgatttt gtgacacact caaggttacc agagaccacg gtggtctacc
      481 ctgcggacgt ggttctgttt gagggcatct tggtgttcta cagccaggag atccgggaca
      541 tgttccacct gcgcctcttc gtggacaccg actccgacgt caggctgtct cgaagagttc
      601 tccgggacgt gcgccgaggg agggacctgg agcagattct gacgcagtac accaccttcg
      661 tgaagccggc cttcgaggag ttctgcctgc cgacaaagaa gtatgccgat gtgatcatcc
      721 cgcgaggagt ggacaatatg gttgccatca acctgatcgt gcagcacatc caggacattc
      781 tgaatggtga catctgcaaa tggcaccgag gagggtccaa tgggcggagc tacaagcgga
      841 ccttttctga gccaggggac caccctggga tgctgacctc tggcaaacgg tcacatttgg
      901 agtccagcag cagaccccac tgaggggctg ccgagcctca gggcaggtct cccgcccggc
      961 atgtgtgttc agggactgag cctggggacg cccacccaca cccactgctt cctctcggcg
     1021 caccccaggg gagtgttagc agcgaggcct tcctcactca ggagtggaaa ctcagatgtg
     1081 tcactcagac tcaacttgct gggacactga caggcgttcc tgaggttttc agccacttag
     1141 gctcgttgcg gtttaaagat ccctctaggt cactgagaaa tgccacagaa tgtgcaggaa
     1201 gcctgggagg cttctgtgag gaatgtgagg cacattattg gggaaattga ggagacagcc
     1261 tagacactgg ctggcctgat gttttgttga cagtgaaccc acagtgggag agagtttttt
     1321 ccagtctgat ctggttctta cacactcaca cacataactc aaaagttttg tgaacaagta
     1381 ctttcctttt ttacatgtta catgtcctca tgttttctgt tttctgtttc ataacacaag
     1441 gctggttgtg gcctacaaac ctaatttcat gacccagtgg tttgcagtcc agcgtggcct
     1501 acacggatat ggggagccac tgagggatgt tttcccccct tgcttgtgcc ttaaaggcag
     1561 agaagcgagg cggatgccct ggaagcaccc agcatcacac ccaggcttgt gcggggccag
     1621 gctgggagcc catgcagtag ggcagaaggc agcggaggcc aggtctgtcc cggctggaga
     1681 acagcgtcca cgcagtcctg cctggggtca ggccctcact gaccctcagg ggagccccca
     1741 aggtgctgtc tgtctagaca ggctgtccca ccccagggtg gctagtggcc atatgcaggg
     1801 aatggtgctt ctggctgtgg cacgcactgg atcacccagg tcccagcaga catggccgcc
     1861 caaagtcagc aagcctcctt tgtgctatgt ggagctcaca gcctcacatg tgaacacccg
     1921 tgtctgggtt gcctggggtg atcctccctc ctgcgtggtg gctgtctctg gaaagcatcc
     1981 cttgccgctg ccatgggcag ccccagcccc cgtccgtcca ggctcaccca cagtagtgat
     2041 gcagacgtga cgtgggggaa gggggctgag ccctgtggct gggttctgac aactgtaacg
     2101 gttttgtcga gcttaggccc ctttggaggg agaatcaata aataacaaac accaactaca
     2161 aa
//
