LOCUS       NM_001199263            2139 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens ubiquitin C-terminal hydrolase L5 (UCHL5), transcript
            variant 4, mRNA.
ACCESSION   NM_001199263 XR_108323 XR_111979 XR_114956
VERSION     NM_001199263.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Wan,B., Cheng,M., He,T. and Zhang,L.
  TITLE     UCHL5 promotes hepatocellular carcinoma progression by promoting
            glycolysis through activating Wnt/beta-catenin pathway
  JOURNAL   BMC Cancer 24 (1), 618 (2024)
   PUBMED   38773433
  REMARK    GeneRIF: UCHL5 promotes hepatocellular carcinoma progression by
            promoting glycolysis through activating Wnt/beta-catenin pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Hashimoto,K., Miyagawa,Y., Watanabe,S., Takasaki,K., Nishizawa,M.,
            Yatsuki,K., Takahashi,Y., Kamata,H., Kihira,C., Hiraike,H.,
            Sasamori,Y., Kido,K., Ryo,E. and Nagasaka,K.
  TITLE     The TGF-beta/UCHL5/Smad2 Axis Contributes to the Pathogenesis of
            Placenta Accreta
  JOURNAL   Int J Mol Sci 24 (18), 13706 (2023)
   PUBMED   37762005
  REMARK    GeneRIF: The TGF-beta/UCHL5/Smad2 Axis Contributes to the
            Pathogenesis of Placenta Accreta.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2139)
  AUTHORS   Yang,Y., Cao,L., Guo,Z., Gu,H., Zhang,K. and Qiu,Z.
  TITLE     Deubiquitinase UCHL5 stabilizes ELK3 to potentiate cancer stemness
            and tumor progression in pancreatic adenocarcinoma (PAAD)
  JOURNAL   Exp Cell Res 421 (2), 113402 (2022)
   PUBMED   36328194
  REMARK    GeneRIF: Deubiquitinase UCHL5 stabilizes ELK3 to potentiate cancer
            stemness and tumor progression in pancreatic adenocarcinoma (PAAD).
REFERENCE   4  (bases 1 to 2139)
  AUTHORS   van der Wal,L., Bezstarosti,K. and Demmers,J.A.A.
  TITLE     A ubiquitinome analysis to study the functional roles of the
            proteasome associated deubiquitinating enzymes USP14 and UCH37
  JOURNAL   J Proteomics 262, 104592 (2022)
   PUBMED   35489684
  REMARK    GeneRIF: A ubiquitinome analysis to study the functional roles of
            the proteasome associated deubiquitinating enzymes USP14 and UCH37.
REFERENCE   5  (bases 1 to 2139)
  AUTHORS   Xiang,L., Zeng,Q., Liu,J., Xiao,M., He,D., Zhang,Q., Xie,D.,
            Deng,M., Zhu,Y., Liu,Y., Bo,H., Liu,X., Zhou,M., Xiong,W., Zhou,Y.,
            Zhou,J., Li,X. and Cao,K.
  TITLE     MAFG-AS1/MAFG positive feedback loop contributes to cisplatin
            resistance in bladder urothelial carcinoma through antagonistic
            ferroptosis
  JOURNAL   Sci Bull (Beijing) 66 (17), 1773-1788 (2021)
   PUBMED   36654385
  REMARK    GeneRIF: MAFG-AS1/MAFG positive feedback loop contributes to
            cisplatin resistance in bladder urothelial carcinoma through
            antagonistic ferroptosis.
REFERENCE   6  (bases 1 to 2139)
  AUTHORS   Rolen,U., Kobzeva,V., Gasparjan,N., Ovaa,H., Winberg,G.,
            Kisseljov,F. and Masucci,M.G.
  TITLE     Activity profiling of deubiquitinating enzymes in cervical
            carcinoma biopsies and cell lines
  JOURNAL   Mol Carcinog 45 (4), 260-269 (2006)
   PUBMED   16402389
  REMARK    GeneRIF: Upregulation of UCH37 is associated with Uterine Cervical
            Neoplasms
REFERENCE   7  (bases 1 to 2139)
  AUTHORS   Volik,S., Raphael,B.J., Huang,G., Stratton,M.R., Bignel,G.,
            Murnane,J., Brebner,J.H., Bajsarowicz,K., Paris,P.L., Tao,Q.,
            Kowbel,D., Lapuk,A., Shagin,D.A., Shagina,I.A., Gray,J.W.,
            Cheng,J.F., de Jong,P.J., Pevzner,P. and Collins,C.
  TITLE     Decoding the fine-scale structure of a breast cancer genome and
            transcriptome
  JOURNAL   Genome Res 16 (3), 394-404 (2006)
   PUBMED   16461635
REFERENCE   8  (bases 1 to 2139)
  AUTHORS   Wicks,S.J., Haros,K., Maillard,M., Song,L., Cohen,R.E., Dijke,P.T.
            and Chantry,A.
  TITLE     The deubiquitinating enzyme UCH37 interacts with Smads and
            regulates TGF-beta signalling
  JOURNAL   Oncogene 24 (54), 8080-8084 (2005)
   PUBMED   16027725
  REMARK    GeneRIF: UCH37 interacts with Smads and regulates TGF-beta
            signalling.
REFERENCE   9  (bases 1 to 2139)
  AUTHORS   Li,T., Duan,W., Yang,H., Lee,M.K., Bte Mustafa,F., Lee,B.H. and
            Teo,T.S.
  TITLE     Identification of two proteins, S14 and UIP1, that interact with
            UCH37
  JOURNAL   FEBS Lett 488 (3), 201-205 (2001)
   PUBMED   11163772
REFERENCE   10 (bases 1 to 2139)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA255130.1, AF151828.1, BC025369.2, AL136370.4 and DN916480.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001199263.2.
            
            Transcript Variant: This variant (4) uses an alternate in-frame
            splice site in the central coding region and uses a distinct splice
            pattern in the 3' coding region and 3' UTR, compared to variant 1.
            The resulting isoform (4) lacks one internal residue and has a
            shorter and distinct C-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.583933.1,
                                           SRR14038194.1266523.1 [ECO:0000332]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               DA255130.1         34-156
            124-688             AF151828.1         37-601
            689-689             BC025369.2         700-700
            690-1168            AF151828.1         603-1081
            1169-1458           BC025369.2         1255-1544
            1459-2083           AL136370.4         103215-103839
            2084-2136           DN916480.1         1-53                c
            2137-2139           AL136370.4         103893-103895
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q31.2"
     gene            1..2139
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="ubiquitin C-terminal hydrolase L5"
                     /db_xref="GeneID:51377"
                     /db_xref="HGNC:HGNC:19678"
                     /db_xref="MIM:610667"
     exon            1..176
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..1051
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /EC_number="3.4.19.12"
                     /note="isoform 4 is encoded by transcript variant 4;
                     ubiquitin C-terminal hydrolase UCH37; ubiquitin
                     carboxyl-terminal esterase L5; INO80 complex subunit R;
                     ubiquitin carboxyl-terminal hydrolase isozyme L5;
                     ubiquitin thioesterase L5; ubiquitin carboxyl-terminal
                     hydrolase L5"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase isozyme L5
                     isoform 4"
                     /protein_id="NP_001186192.1"
                     /db_xref="CCDS:CCDS55669.1"
                     /db_xref="GeneID:51377"
                     /db_xref="HGNC:HGNC:19678"
                     /db_xref="MIM:610667"
                     /translation="MTGNAGEWCLMESDPGVFTELIKGFGCRGAQVEEIWSLEPENFE
                     KLKPVHGLIFLFKWQPGEEPAGSVVQDSRLDTIFFAKQVINNACATQAIVSVLLNCTH
                     QDVHLGETLSEFKEFSQSFDAAMKGLALSNSDVIRQVHNSFARQQMFEFDTKTSAKEE
                     DAFHFVSYVPVNGRLYELDGLREGPIDLGACNQDDWISAVRPVIEKRIQKYSEGEIRF
                     NLMAIVSDRKMIYEQKIAELQRQLAEEPMDTDQGNSMLSAIQSEVAKNQMLIEEEVQK
                     LKRYKIENIRRKHNYLPFIMELLKTLAEHQQLIPLVEKGK"
     exon            177..240
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            241..346
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            347..472
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            473..534
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            535..665
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            666..729
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            730..832
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            833..943
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1042
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..2139
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1434..1439
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="hexamer: AATAAA"
     polyA_site      1458
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
     regulatory      2114..2119
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="hexamer: AATAAA"
     polyA_site      2139
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="major polyA site"
ORIGIN      
        1 gagctcggcg gcgggtttgc tgagatctgt ggccggcggc agctggtgcg gggggcagct
       61 gagagcgaga ggtggatcgg ggcggtgtgt ggccagggcc atgacgggca atgccgggga
      121 gtggtgcctc atggaaagcg accccggggt cttcaccgag ctcattaaag gattcggttg
      181 ccgaggagcc caagtagaag aaatatggag tttagagcct gagaattttg aaaaattaaa
      241 gccagttcat gggttaattt ttcttttcaa gtggcagcca ggagaagaac cagcaggctc
      301 tgtggttcag gactcccgac ttgacacgat attttttgct aagcaggtaa ttaataatgc
      361 ttgtgctact caagccatag tgagtgtgtt actgaactgt acccaccagg atgtccattt
      421 aggcgagaca ttatcagagt ttaaagaatt ttcacaaagt tttgatgcag ctatgaaagg
      481 cttggcactg agcaattcag atgtgattcg acaagtacac aacagtttcg ccagacagca
      541 aatgtttgaa tttgatacga agacatcagc aaaagaagaa gatgcttttc actttgtcag
      601 ttatgttcct gttaatggga gactgtatga attagatgga ttaagagaag gaccgattga
      661 tttaggtgca tgcaatcaag atgattggat cagtgcagta aggcctgtca tagaaaaaag
      721 gatacaaaag tacagtgaag gtgaaattcg atttaattta atggccattg tgtctgacag
      781 aaaaatgata tatgagcaga agatagcaga gttacaaaga caacttgcag aggaacccat
      841 ggatacagat caaggtaata gtatgttaag tgctattcag tcagaagttg ccaaaaatca
      901 gatgcttatt gaagaagaag tacagaaatt aaaaagatac aagattgaga atatcagaag
      961 gaagcataat tatctgcctt tcattatgga attgttaaag actttagcag aacaccagca
     1021 gttaatacca ctagtagaaa agggaaaata ggataaaaga acaaggtgtg agaaggaata
     1081 gaaggaaaca aacaggaaag atatggctgc accatgcagt gctactatat gctgagattc
     1141 tacaggatga gatttttgaa tagctgaggc agttgcctat aatctgtgat gacataaaag
     1201 tatttgacct aaaatctttt tatttgcaaa ataataaata aaaagtgatt ctccctcggc
     1261 atggctataa tgaacttggt tagaacctat aaatgttttt gttatgtgag actttttaac
     1321 cgatgaattc gttgaataca caccacattt aaaaactgcc taggaggtaa tccaaaaata
     1381 tatagttata aaacttcttg tattatgtac caaatctctt tcttaaaata taaaataaaa
     1441 ttatctagtt taagattaca tttgtgagac ttctatttat tcaggtatat ttatgattaa
     1501 caaatttttg tctacacatt tgtatgtaat tatgaatcat tgtctatctt aaatttttaa
     1561 ctaccaataa cttaacgtga tgtaaaatca taaattccat tgctatgggg aaatccaaac
     1621 aacttcacaa tattgaattt tgtccacatt ttactatgac tttgtgattc aattggtaat
     1681 aagtaagctt cagatctttt tctatttctt aatagaaata aggtagaaga atagttaagg
     1741 ctaatcacaa caatatctac attagtatct attaatgcag aattatctga tttgagtcag
     1801 tactttttaa tatcattggt acactgtata gaaggaaagt atttttaaca gtatatattt
     1861 ttccatgtta tttatatgat tttattttta ttttttaaca aaaacagacc aaaatacagg
     1921 ctttgaattt aaatcttata tatatgttac ttattagctg gagcttttag gcactattta
     1981 ctttcttata acctctttct catctgtaaa ctgggaatca tacctacttc acattcttga
     2041 tgattaaatg agatcatatc tgtaaagtgt ataaattagt gctcaattaa ttgtgataat
     2101 tatttttaat tttaataaat gtatagacac tctgcctta
//
