LOCUS       NM_001199261            5184 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens ubiquitin C-terminal hydrolase L5 (UCHL5), transcript
            variant 2, mRNA.
ACCESSION   NM_001199261
VERSION     NM_001199261.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Wan,B., Cheng,M., He,T. and Zhang,L.
  TITLE     UCHL5 promotes hepatocellular carcinoma progression by promoting
            glycolysis through activating Wnt/beta-catenin pathway
  JOURNAL   BMC Cancer 24 (1), 618 (2024)
   PUBMED   38773433
  REMARK    GeneRIF: UCHL5 promotes hepatocellular carcinoma progression by
            promoting glycolysis through activating Wnt/beta-catenin pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Hashimoto,K., Miyagawa,Y., Watanabe,S., Takasaki,K., Nishizawa,M.,
            Yatsuki,K., Takahashi,Y., Kamata,H., Kihira,C., Hiraike,H.,
            Sasamori,Y., Kido,K., Ryo,E. and Nagasaka,K.
  TITLE     The TGF-beta/UCHL5/Smad2 Axis Contributes to the Pathogenesis of
            Placenta Accreta
  JOURNAL   Int J Mol Sci 24 (18), 13706 (2023)
   PUBMED   37762005
  REMARK    GeneRIF: The TGF-beta/UCHL5/Smad2 Axis Contributes to the
            Pathogenesis of Placenta Accreta.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5184)
  AUTHORS   Yang,Y., Cao,L., Guo,Z., Gu,H., Zhang,K. and Qiu,Z.
  TITLE     Deubiquitinase UCHL5 stabilizes ELK3 to potentiate cancer stemness
            and tumor progression in pancreatic adenocarcinoma (PAAD)
  JOURNAL   Exp Cell Res 421 (2), 113402 (2022)
   PUBMED   36328194
  REMARK    GeneRIF: Deubiquitinase UCHL5 stabilizes ELK3 to potentiate cancer
            stemness and tumor progression in pancreatic adenocarcinoma (PAAD).
REFERENCE   4  (bases 1 to 5184)
  AUTHORS   van der Wal,L., Bezstarosti,K. and Demmers,J.A.A.
  TITLE     A ubiquitinome analysis to study the functional roles of the
            proteasome associated deubiquitinating enzymes USP14 and UCH37
  JOURNAL   J Proteomics 262, 104592 (2022)
   PUBMED   35489684
  REMARK    GeneRIF: A ubiquitinome analysis to study the functional roles of
            the proteasome associated deubiquitinating enzymes USP14 and UCH37.
REFERENCE   5  (bases 1 to 5184)
  AUTHORS   Xiang,L., Zeng,Q., Liu,J., Xiao,M., He,D., Zhang,Q., Xie,D.,
            Deng,M., Zhu,Y., Liu,Y., Bo,H., Liu,X., Zhou,M., Xiong,W., Zhou,Y.,
            Zhou,J., Li,X. and Cao,K.
  TITLE     MAFG-AS1/MAFG positive feedback loop contributes to cisplatin
            resistance in bladder urothelial carcinoma through antagonistic
            ferroptosis
  JOURNAL   Sci Bull (Beijing) 66 (17), 1773-1788 (2021)
   PUBMED   36654385
  REMARK    GeneRIF: MAFG-AS1/MAFG positive feedback loop contributes to
            cisplatin resistance in bladder urothelial carcinoma through
            antagonistic ferroptosis.
REFERENCE   6  (bases 1 to 5184)
  AUTHORS   Rolen,U., Kobzeva,V., Gasparjan,N., Ovaa,H., Winberg,G.,
            Kisseljov,F. and Masucci,M.G.
  TITLE     Activity profiling of deubiquitinating enzymes in cervical
            carcinoma biopsies and cell lines
  JOURNAL   Mol Carcinog 45 (4), 260-269 (2006)
   PUBMED   16402389
  REMARK    GeneRIF: Upregulation of UCH37 is associated with Uterine Cervical
            Neoplasms
REFERENCE   7  (bases 1 to 5184)
  AUTHORS   Volik,S., Raphael,B.J., Huang,G., Stratton,M.R., Bignel,G.,
            Murnane,J., Brebner,J.H., Bajsarowicz,K., Paris,P.L., Tao,Q.,
            Kowbel,D., Lapuk,A., Shagin,D.A., Shagina,I.A., Gray,J.W.,
            Cheng,J.F., de Jong,P.J., Pevzner,P. and Collins,C.
  TITLE     Decoding the fine-scale structure of a breast cancer genome and
            transcriptome
  JOURNAL   Genome Res 16 (3), 394-404 (2006)
   PUBMED   16461635
REFERENCE   8  (bases 1 to 5184)
  AUTHORS   Wicks,S.J., Haros,K., Maillard,M., Song,L., Cohen,R.E., Dijke,P.T.
            and Chantry,A.
  TITLE     The deubiquitinating enzyme UCH37 interacts with Smads and
            regulates TGF-beta signalling
  JOURNAL   Oncogene 24 (54), 8080-8084 (2005)
   PUBMED   16027725
  REMARK    GeneRIF: UCH37 interacts with Smads and regulates TGF-beta
            signalling.
REFERENCE   9  (bases 1 to 5184)
  AUTHORS   Li,T., Duan,W., Yang,H., Lee,M.K., Bte Mustafa,F., Lee,B.H. and
            Teo,T.S.
  TITLE     Identification of two proteins, S14 and UIP1, that interact with
            UCH37
  JOURNAL   FEBS Lett 488 (3), 201-205 (2001)
   PUBMED   11163772
REFERENCE   10 (bases 1 to 5184)
  AUTHORS   Hu,R.M., Han,Z.G., Song,H.D., Peng,Y.D., Huang,Q.H., Ren,S.X.,
            Gu,Y.J., Huang,C.H., Li,Y.B., Jiang,C.L., Fu,G., Zhang,Q.H.,
            Gu,B.W., Dai,M., Mao,Y.F., Gao,G.F., Rong,R., Ye,M., Zhou,J.,
            Xu,S.H., Gu,J., Shi,J.X., Jin,W.R., Zhang,C.K., Wu,T.M.,
            Huang,G.Y., Chen,Z., Chen,M.D. and Chen,J.L.
  TITLE     Gene expression profiling in the human
            hypothalamus-pituitary-adrenal axis and full-length cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 97 (17), 9543-9548 (2000)
   PUBMED   10931946
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA255130.1, BC015521.1, AL136370.4 and BP397519.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001199261.2.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the central coding region, compared to variant 1.
            The resulting isoform (2) lacks one internal residue, compared to
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.174826.1,
                                           SRR1660807.193863.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367454.6/ ENSP00000356424.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                DA255130.1         34-67
            35-1483             BC015521.1         1-1449
            1484-4915           AL136370.4         105665-109096
            4916-5184           BP397519.1         231-499
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q31.2"
     gene            1..5184
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="ubiquitin C-terminal hydrolase L5"
                     /db_xref="GeneID:51377"
                     /db_xref="HGNC:HGNC:19678"
                     /db_xref="MIM:610667"
     exon            1..176
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..1087
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /EC_number="3.4.19.12"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ubiquitin C-terminal hydrolase UCH37; ubiquitin
                     carboxyl-terminal esterase L5; INO80 complex subunit R;
                     ubiquitin carboxyl-terminal hydrolase isozyme L5;
                     ubiquitin thioesterase L5; ubiquitin carboxyl-terminal
                     hydrolase L5"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase isozyme L5
                     isoform 2"
                     /protein_id="NP_001186190.1"
                     /db_xref="CCDS:CCDS55668.1"
                     /db_xref="GeneID:51377"
                     /db_xref="HGNC:HGNC:19678"
                     /db_xref="MIM:610667"
                     /translation="MTGNAGEWCLMESDPGVFTELIKGFGCRGAQVEEIWSLEPENFE
                     KLKPVHGLIFLFKWQPGEEPAGSVVQDSRLDTIFFAKQVINNACATQAIVSVLLNCTH
                     QDVHLGETLSEFKEFSQSFDAAMKGLALSNSDVIRQVHNSFARQQMFEFDTKTSAKEE
                     DAFHFVSYVPVNGRLYELDGLREGPIDLGACNQDDWISAVRPVIEKRIQKYSEGEIRF
                     NLMAIVSDRKMIYEQKIAELQRQLAEEPMDTDQGNSMLSAIQSEVAKNQMLIEEEVQK
                     LKRYKIENIRRKHNYLPFIMELLKTLAEHQQLIPLVEKAKEKQNAKKAQETK"
     exon            177..240
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            241..346
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            347..472
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            473..534
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            535..665
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            666..729
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            730..832
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            833..943
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1042
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..5184
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1934..1939
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="hexamer: AATAAA"
     polyA_site      1955
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="major polyA site"
     regulatory      3086..3091
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="hexamer: AATAAA"
     polyA_site      3106
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
     regulatory      3197..3202
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="hexamer: AATAAA"
     polyA_site      3226
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
     regulatory      5045..5050
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="hexamer: AATAAA"
     polyA_site      5072
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
     regulatory      5163..5168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
                     /note="hexamer: AATAAA"
     polyA_site      5184
                     /gene="UCHL5"
                     /gene_synonym="CGI-70; INO80R; UCH-L5; UCH37"
ORIGIN      
        1 gagctcggcg gcgggtttgc tgagatctgt ggccggcggc agctggtgcg gggggcagct
       61 gagagcgaga ggtggatcgg ggcggtgtgt ggccagggcc atgacgggca atgccgggga
      121 gtggtgcctc atggaaagcg accccggggt cttcaccgag ctcattaaag gattcggttg
      181 ccgaggagcc caagtagaag aaatatggag tttagagcct gagaattttg aaaaattaaa
      241 gccagttcat gggttaattt ttcttttcaa gtggcagcca ggagaagaac cagcaggctc
      301 tgtggttcag gactcccgac ttgacacgat attttttgct aagcaggtaa ttaataatgc
      361 ttgtgctact caagccatag tgagtgtgtt actgaactgt acccaccagg atgtccattt
      421 aggcgagaca ttatcagagt ttaaagaatt ttcacaaagt tttgatgcag ctatgaaagg
      481 cttggcactg agcaattcag atgtgattcg acaagtacac aacagtttcg ccagacagca
      541 aatgtttgaa tttgatacga agacatcagc aaaagaagaa gatgcttttc actttgtcag
      601 ttatgttcct gttaatggga gactgtatga attagatgga ttaagagaag gaccgattga
      661 tttaggtgca tgcaatcaag atgattggat cagtgcagta aggcctgtca tagaaaaaag
      721 gatacaaaag tacagtgaag gtgaaattcg atttaattta atggccattg tgtctgacag
      781 aaaaatgata tatgagcaga agatagcaga gttacaaaga caacttgcag aggaacccat
      841 ggatacagat caaggtaata gtatgttaag tgctattcag tcagaagttg ccaaaaatca
      901 gatgcttatt gaagaagaag tacagaaatt aaaaagatac aagattgaga atatcagaag
      961 gaagcataat tatctgcctt tcattatgga attgttaaag actttagcag aacaccagca
     1021 gttaatacca ctagtagaaa aggcaaaaga aaaacagaac gcaaagaaag ctcaggaaac
     1081 caaatgaaga tgttttcaga tatgtacaca tttctgcttc tgcacatatt ttcatggaaa
     1141 ccattatgta taaagaactt agagcaacat cctaattggc tcagtgcacg tttggcaata
     1201 gtgccagcct gtcttgtctt taatgcatgg attcataaac ttcttcccta cctgcatcat
     1261 gtgcatgtag tgcatattaa atgaaagtga tattaagaat gctttcccaa attccattat
     1321 ttgacattga gtctgacaac tgttagtttt ctggttgtct aactaccata tgaagctaga
     1381 aaatgcacaa acgatattcc ttatctgtaa tttaaatact taaaatttgc aattgtcaga
     1441 tcttgattaa actggttgtc ttatttcttc tcatcattaa cggaaaaaaa atcagtattt
     1501 ctatctttga tatctaagtg ttttgaggat tttaaaactg aattttatct gctataccag
     1561 ttatttgaga aagtatgatt ttaatgtaaa tcatttaaaa aggacaaaag tataatttcc
     1621 agtgattttc actgctgtca gtagaaaagt aataaacatc tcaattttat tttagtaaat
     1681 tttcttcaag tgtttggggt tatttgttta tgtattagag aattgtttca ggaaggtctg
     1741 agtattatgc ttcaaagcaa aatttcaggt taagaagaaa ttgtaaatct taaagaatgt
     1801 tggtgttact ctcaatggaa tattgtttca agcttgtaag ctgtgtataa aaaaactgga
     1861 ggtctgacag ttcatgttat ctgctttttt aaaaagatgg tagtggtgat gggggtcttt
     1921 ctatgccaat ttgaataaat ttcgtacagc ctgcagtttt caagagccat atgtaatttg
     1981 ctcacaattg atttttaaaa attgatttgg gagttgcttt gtaataatct tctgcttcta
     2041 atcttccttt gcaagtcaga ctttaaagaa gactttataa agctttcttt tttaaaggaa
     2101 gtaacctact tttttcttta tcaagaatat ttctgaggtt gatacatgac tttaacattt
     2161 tattatttat tgtaaaatca atttagcata atttatttta ttagggagaa tgtgtaccca
     2221 tcttccagct tcagcacatt tttaaggatt gtttttaagc ttagtaattg tggtattaaa
     2281 tgacttagaa ctaatggaga aaaacctgat aggaaattga tagaaaaacg aatgaaatat
     2341 ttttcataca taatttcagt tggaggtaat ggaaaatatg tagtctctca gttatttgtg
     2401 ggtaaatatg ttttgctgtt gatacagtaa taattatgga gtgttttcaa agccatgata
     2461 taatttttaa aaatagattt ttaaggaagg ctcatcttga tctcattcgt ttatttcagc
     2521 ttaatttata tattctttcc tattgaggct taaattttgt taagcaatgt gaggattatt
     2581 gtggtaagtt gtatttagta atcaatacag tgtgatactg gcactaggat tgacaaataa
     2641 agcaatggaa cagaagagag agcctgaaaa cagatcaacg tttatatgat cacttgattt
     2701 cgacaaaggt ggcaaaacaa ttcaacagag aaggaaagtc ttttcaacaa atggagccag
     2761 aatagaatag ctgaatatcc atatagaaaa aagtgaacaa tgacccccta cctcacaaaa
     2821 atgactttga gatgaatcat agacctaaat gtaatatttt aaaagtatga agctttttaa
     2881 ctgaaaacag atgatatttt tgtgacttgg ggggttagcc aaaggtttct taggtcacag
     2941 aaagcagtaa ccataaaaga tccaaaatta gagctcatca gaattgtcat tgaaagtgtt
     3001 aacaaaagta atatcaaatg acatttgaaa aatatccttg taaaatgact atattaatag
     3061 gtataaggat ttttcttatt aatctaataa aattccttaa taggaatttc catagcttaa
     3121 gagtggttta ttttatctgc atcttctttg gtgattgcaa atcccttgtg atacttcatg
     3181 attacttctg tctgtgaata aagaagtgtg acttgtctgg gtgctatatg ttgctggttt
     3241 gaaattaagt catgaagcta gagggtagag aaaatggaag aataaagagc aacccataaa
     3301 tagataaatg ctatttggaa agtttttttt aaatgatatt ttatagaaaa tcttgctcat
     3361 ttcatattta gtcttttcta aaattttatt taattattgg gtaggtgaag tcgcttccat
     3421 tctattttaa gccttcaaaa tagccaattc taagggatgt acttctgtta ttatcaacaa
     3481 aaaccttgcc aacagctgcg gcactggcta ctctcacctt atatgtttag ttcccaagat
     3541 agcttgccct tttccgaaca gcagtcagct cgactgtgcc actaaaacag acaaatattt
     3601 gctcgggaat cacaaccacg gggacttgct cccccagtta ggaccatggt acatatttgt
     3661 gtgtatatta tggtgttaca tgcagattaa tactttcaat taatcctcct agttgcctgt
     3721 aacgttaaca tttcaagatg catttagata tttttatcct gtaggaggat tttgtttatt
     3781 tgagggaaaa aaagggcttt taatgtattc tcctcaaaaa ccatttagag aaaacagata
     3841 agtaaaaata aaatttaaat taccatattt ctatttacag ggatgagcac attaacattt
     3901 tatgtattta gtgatccttt ttcctcatgt gtacacatat gtttttgtgt gttagtcttg
     3961 cttgccctcc ccatagtctg aaatagttct atgaagttta tattattttt aaacttgatc
     4021 atatacaaat tttcaggaaa caaaccactc tagctatttg aagaggaatg cagatttata
     4081 ttgggagttt tgaaactaca tagtttctta caaaatcatt ggaaggtttg gaggagcggt
     4141 ttcaagctta agtctccaga gattactccc atccaaacat acagtattgg tgtacctagg
     4201 gaactgctca cttttgcaac agtcagggag ttcactgttg gaactattaa tgttagaaca
     4261 tgttgcagta gttgtgttca aggaatgagg aacctgctgt aacacttaat gactgtgcac
     4321 tggaatgcag aaaagttaaa ccttctatgc ctaaatgcct aaaacagaca aactggatag
     4381 ttatattttt agaatgatac tgcgtttttt agagtagtat gaattcctag atcagtcaaa
     4441 catgcatgtt atttagtttg ttctttagta tattgttaac tggcctaaat ccaatgactg
     4501 acatggtaaa gattcaccac ttctagcata ctgggcactt tttatttttg acttgattac
     4561 attaactttt tgaaaactat tcagtgattt attcagccaa tcttatgagg tacatctgag
     4621 ttgttctaga tgctagagga tagtataaca ggaaaaagcc cctgctctca tggtgagaca
     4681 gataataagt atgtaataca acttttcaaa tagtagtatg aatggtaaaa aaaataggga
     4741 aaaggggtgg gaggagctga ggtttgatag aatggtcatg gaagatccca ttaatgagat
     4801 gtactttaag tagagaccta aatgaagata attttttctc tagtactgaa ctagcactgt
     4861 acaagtggat tgcatatagg catataggca gaacttctct agtactgaac taacactgta
     4921 caagtggatt gcatataggc atataggcag aaccaatgtg caataattaa gaatattaat
     4981 ttgcaatcat gtttgatggt taaatggttt attaatgttg gagtgtcaat atgaaaatgg
     5041 gttgaataaa agatatatgg aaatgcctag gactactgct ctatttactt tttagcttat
     5101 ccatatattt tatgtaatat gcttctttga ttctttcttg tagcaatgat aatataatat
     5161 tgaataaacc ataaaaccag tggg
//
