LOCUS       NM_006002                931 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens ubiquitin C-terminal hydrolase L3 (UCHL3), transcript
            variant 2, mRNA.
ACCESSION   NM_006002
VERSION     NM_006002.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Lei,H., Xu,H. and Wu,Y.
  TITLE     Role of UCHL3 in health and disease
  JOURNAL   Biochem Biophys Res Commun 734, 150626 (2024)
   PUBMED   39226739
  REMARK    GeneRIF: Role of UCHL3 in health and disease.
            Review article
REFERENCE   2  (bases 1 to 931)
  AUTHORS   Zhao,J., Huo,Q., Zhang,J., Sun,K., Guo,J., Cheng,F., Hu,X. and
            Xu,Q.
  TITLE     UCHL3 promotes hepatocellular carcinoma progression by stabilizing
            EEF1A1 through deubiquitination
  JOURNAL   Biol Direct 19 (1), 53 (2024)
   PUBMED   38965582
  REMARK    GeneRIF: UCHL3 promotes hepatocellular carcinoma progression by
            stabilizing EEF1A1 through deubiquitination.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 931)
  AUTHORS   Shan,W., Peng,W., Chen,Y., Wang,Y., Yu,Q., Tian,Y., Dou,Y., Tu,J.,
            Huang,X., Li,X., Wang,Z., Zhu,Q., Chen,J. and Xia,B.
  TITLE     GSK3beta and UCHL3 govern RIPK4 homeostasis via deubiquitination to
            enhance tumor metastasis in ovarian cancer
  JOURNAL   Oncogene 43 (25), 1885-1899 (2024)
   PUBMED   38664501
  REMARK    GeneRIF: GSK3beta and UCHL3 govern RIPK4 homeostasis via
            deubiquitination to enhance tumor metastasis in ovarian cancer.
REFERENCE   4  (bases 1 to 931)
  AUTHORS   Xie,G., Lei,B., Yin,Z., Xu,F. and Liu,X.
  TITLE     CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2
            Degradation via Interacting with UCHL3
  JOURNAL   Int J Mol Sci 25 (5), 2817 (2024)
   PUBMED   38474064
  REMARK    GeneRIF: CircMTA2 Drives Gastric Cancer Progression through
            Suppressing MTA2 Degradation via Interacting with UCHL3.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 931)
  AUTHORS   Nam,M.J., Madoz-Gurpide,J., Wang,H., Lescure,P., Schmalbach,C.E.,
            Zhao,R., Misek,D.E., Kuick,R., Brenner,D.E. and Hanash,S.M.
  TITLE     Molecular profiling of the immune response in colon cancer using
            protein microarrays: occurrence of autoantibodies to ubiquitin
            C-terminal hydrolase L3
  JOURNAL   Proteomics 3 (11), 2108-2115 (2003)
   PUBMED   14595809
REFERENCE   6  (bases 1 to 931)
  AUTHORS   Wilkinson,K.D., Laleli-Sahin,E., Urbauer,J., Larsen,C.N.,
            Shih,G.H., Haas,A.L., Walsh,S.T. and Wand,A.J.
  TITLE     The binding site for UCH-L3 on ubiquitin: mutagenesis and NMR
            studies on the complex between ubiquitin and UCH-L3
  JOURNAL   J Mol Biol 291 (5), 1067-1077 (1999)
   PUBMED   10518943
REFERENCE   7  (bases 1 to 931)
  AUTHORS   Wada,H., Kito,K., Caskey,L.S., Yeh,E.T. and Kamitani,T.
  TITLE     Cleavage of the C-terminus of NEDD8 by UCH-L3
  JOURNAL   Biochem Biophys Res Commun 251 (3), 688-692 (1998)
   PUBMED   9790970
REFERENCE   8  (bases 1 to 931)
  AUTHORS   Larsen,C.N., Krantz,B.A. and Wilkinson,K.D.
  TITLE     Substrate specificity of deubiquitinating enzymes: ubiquitin
            C-terminal hydrolases
  JOURNAL   Biochemistry 37 (10), 3358-3368 (1998)
   PUBMED   9521656
REFERENCE   9  (bases 1 to 931)
  AUTHORS   Johnston,S.C., Larsen,C.N., Cook,W.J., Wilkinson,K.D. and Hill,C.P.
  TITLE     Crystal structure of a deubiquitinating enzyme (human UCH-L3) at
            1.8 A resolution
  JOURNAL   EMBO J 16 (13), 3787-3796 (1997)
   PUBMED   9233788
REFERENCE   10 (bases 1 to 931)
  AUTHORS   Wilkinson,K.D., Lee,K.M., Deshpande,S., Duerksen-Hughes,P.,
            Boss,J.M. and Pohl,J.
  TITLE     The neuron-specific protein PGP 9.5 is a ubiquitin
            carboxyl-terminal hydrolase
  JOURNAL   Science 246 (4930), 670-673 (1989)
   PUBMED   2530630
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC018125.2 and AA234624.1.
            
            On Feb 27, 2020 this sequence version replaced NM_006002.4.
            
            Summary: The protein encoded by this gene is a member of the
            deubiquitinating enzyme family. Members of this family are
            proteases that catalyze the removal of ubiquitin from polypeptides
            and are divided into five classes, depending on the mechanism of
            catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon
            amide bond of ubiquitinated proteins to regenerate ubiquitin for
            another catalytic cycle. Alternative splicing results in multiple
            transcript variants that encode different protein isoforms.
            [provided by RefSeq, Aug 2012].
            
            Transcript Variant: This variant (2) contains an alternate exon in
            the 5' coding region and initiates translation at an alternate
            start codon compared to variant 1. The encoded isoform (2) has a
            distinct N-terminus and is longer than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HM005534.1, BC018125.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377595.8/ ENSP00000366819.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-844               BC018125.2         1-844
            845-931             AA234624.1         254-340
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q22.2"
     gene            1..931
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /note="ubiquitin C-terminal hydrolase L3"
                     /db_xref="GeneID:7347"
                     /db_xref="HGNC:HGNC:12515"
                     /db_xref="MIM:603090"
     exon            1..72
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..723
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /EC_number="3.4.19.12"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ubiquitin carboxyl-terminal hydrolase isozyme L3;
                     ubiquitin thiolesterase; ubiquitin thioesterase L3;
                     ubiquitin carboxyl-terminal esterase L3 (ubiquitin
                     thiolesterase); testicular tissue protein Li 221"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase isozyme L3
                     isoform 2"
                     /protein_id="NP_005993.1"
                     /db_xref="CCDS:CCDS9453.1"
                     /db_xref="GeneID:7347"
                     /db_xref="HGNC:HGNC:12515"
                     /db_xref="MIM:603090"
                     /translation="MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSM
                     VPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIH
                     AIANNKDKMHFESGSTLKKFLEESVSMSPEERARYLENYDAIRVTHETSAHEGQTEAP
                     SIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGETSDETLLEDAIEVCKKFMERDPD
                     ELRFNAIALSAA"
     exon            73..84
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /inference="alignment:Splign:2.1.0"
     exon            85..213
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /inference="alignment:Splign:2.1.0"
     exon            214..370
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /inference="alignment:Splign:2.1.0"
     exon            371..456
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /inference="alignment:Splign:2.1.0"
     exon            457..504
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /inference="alignment:Splign:2.1.0"
     exon            505..580
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /inference="alignment:Splign:2.1.0"
     exon            581..639
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /inference="alignment:Splign:2.1.0"
     exon            640..931
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      823..828
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /note="hexamer: ATTAAA"
     polyA_site      844
                     /gene="UCHL3"
                     /gene_synonym="UCH-L3"
                     /note="major polyA site"
ORIGIN      
        1 gtcagagctg gagggccggg caccgcggcc atggagggtc aacgctggct gccgctggag
       61 gccaatcccg aggtcaccaa ccagtttctt aaacaattag gtctacatcc taactggcaa
      121 ttcgttgatg tatatggaat ggatcctgaa ctccttagca tggtaccaag accagtctgt
      181 gcagtcttac ttctctttcc tattacagaa aagtatgaag tattcagaac agaagaggaa
      241 gaaaaaataa aatctcaggg acaagatgtt acatcatcag tatatttcat gaagcaaaca
      301 atcagcaatg cctgtggaac aattggactg attcatgcta ttgcaaacaa taaagacaag
      361 atgcactttg aatctggatc aaccttgaaa aaattcctgg aggaatctgt gtcaatgagc
      421 cctgaagaac gagccagata cctggagaac tatgatgcca tccgagttac tcatgagacc
      481 agtgcccatg aaggtcagac tgaggcacca agtatagatg agaaagtaga tcttcatttt
      541 attgcattag ttcatgtaga tgggcatctc tatgaattag atgggcggaa gccatttcca
      601 attaaccatg gtgaaactag tgatgaaact ttattagagg atgccataga agtttgcaag
      661 aagtttatgg agcgcgaccc tgatgaacta agatttaatg cgattgctct ttctgcagca
      721 tagcttgtca ataatggaaa caccaaaaac tgtattattt gcaactaaat tttctctgcc
      781 atacactaac tcaaaaattt tgatattttc attaacttga tgattaaact ttatgtgagt
      841 taaactttgc cttaacctgt gttttatgtt atttttgctc caggttaaag gtgcaatgct
      901 ttcctcctct tttcttgtga aggatttatc t
//
