LOCUS       NM_005671               1451 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens UBX domain protein 8 (UBXN8), transcript variant 1,
            mRNA.
ACCESSION   NM_005671
VERSION     NM_005671.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Yang,E., Guan,W., Gong,D., Li,J., Han,C., Zhang,J., Wang,H.,
            Kang,S., Gao,X., Li,Y. and Yu,L.
  TITLE     Epigenetic silencing of UBXN8 contributes to leukemogenesis in
            t(8;21) acute myeloid leukemia
  JOURNAL   Exp Mol Med 53 (12), 1902-1910 (2021)
   PUBMED   34921223
  REMARK    GeneRIF: Epigenetic silencing of UBXN8 contributes to
            leukemogenesis in t(8;21) acute myeloid leukemia.
REFERENCE   3  (bases 1 to 1451)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 1451)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1451)
  AUTHORS   Trepte,P., Kruse,S., Kostova,S., Hoffmann,S., Buntru,A.,
            Tempelmeier,A., Secker,C., Diez,L., Schulz,A., Klockmeier,K.,
            Zenkner,M., Golusik,S., Rau,K., Schnoegl,S., Garner,C.C. and
            Wanker,E.E.
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology
            for quantitative mapping of protein-protein interactions in
            mammalian cells
  JOURNAL   Mol Syst Biol 14 (7), e8071 (2018)
   PUBMED   29997244
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1451)
  AUTHORS   Madsen,L., Kriegenburg,F., Vala,A., Best,D., Prag,S., Hofmann,K.,
            Seeger,M., Adams,I.R. and Hartmann-Petersen,R.
  TITLE     The tissue-specific Rep8/UBXD6 tethers p97 to the endoplasmic
            reticulum membrane for degradation of misfolded proteins
  JOURNAL   PLoS One 6 (9), e25061 (2011)
   PUBMED   21949850
REFERENCE   7  (bases 1 to 1451)
  AUTHORS   Alexandru,G., Graumann,J., Smith,G.T., Kolawa,N.J., Fang,R. and
            Deshaies,R.J.
  TITLE     UBXD7 binds multiple ubiquitin ligases and implicates p97 in
            HIF1alpha turnover
  JOURNAL   Cell 134 (5), 804-816 (2008)
   PUBMED   18775313
REFERENCE   8  (bases 1 to 1451)
  AUTHORS   Yamabe,Y., Ichikawa,K., Sugawara,K., Imamura,O., Shimamoto,A.,
            Suzuki,N., Tokutake,Y., Goto,M., Sugawara,M. and Furuichi,Y.
  TITLE     Cloning and characterization of Rep-8 (D8S2298E) in the human
            chromosome 8p11.2-p12
  JOURNAL   Genomics 39 (2), 198-204 (1997)
   PUBMED   9027507
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009314.4 and KF459648.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_005671.3.
            
            Summary: p97 or VCP (valosin-containing protein) is a versatile
            ATPase complex, and many cofactors are required for the p97
            functional diversity. This gene encodes one of the p97 cofactors.
            This cofactor is a transmembrane protein and localized in the
            endoplasmic reticulum (ER) membrane. It tethers p97 to the ER
            membrane via its UBX domain. The association of this cofactor with
            p97 facilitates efficient ER-associated degradation of misfolded
            proteins. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene.[provided by
            RefSeq, Aug 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1), which is a transmembrane protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189658.194162.1,
                                           SRR5189658.166104.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265616.10/ ENSP00000479216.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-114               AC009314.4         95007-95120         c
            115-237             AC009314.4         87766-87888         c
            238-308             AC009314.4         86179-86249         c
            309-431             AC009314.4         84497-84619         c
            432-554             AC009314.4         82397-82519         c
            555-596             AC009314.4         78355-78396         c
            597-671             KF459648.1         78-152
            672-1451            AC009314.4         72279-73058         c
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p12"
     gene            1..1451
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /note="UBX domain protein 8"
                     /db_xref="GeneID:7993"
                     /db_xref="HGNC:HGNC:30307"
                     /db_xref="MIM:602155"
     exon            1..114
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..839
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Reproduction/chromosome 8; UBX domain-containing protein
                     8; rep-8 protein; reproduction 8 protein; UBX
                     domain-containing protein 6"
                     /codon_start=1
                     /product="UBX domain-containing protein 8 isoform 1"
                     /protein_id="NP_005662.2"
                     /db_xref="CCDS:CCDS75723.1"
                     /db_xref="GeneID:7993"
                     /db_xref="HGNC:HGNC:30307"
                     /db_xref="MIM:602155"
                     /translation="MASRGVVGIFFLSAVPLVCLELRRGIPDIGIKDFLLLCGRILLL
                     LALLTLTISVTTSWLNSFKSPQVYLKEEEEKNEKRQKLVRKKQQEAQGEKASRYIENV
                     LKPHQEMKLRKLEERFYQMTGEAWKLSSGHKLGGDEGTSQTSFETSNREAAKSQNLPK
                     PLTEFPSPAEQPTCKEIPDLPEEPSQTAEEVVTVALRCPSGNVLRRRFLKSYSSQVLF
                     DWMTRIGYHISLYSLSTSFPRRPLAVEGGQSLEDIGITVDTVLILEEKEQTN"
     exon            115..237
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /inference="alignment:Splign:2.1.0"
     exon            238..308
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /inference="alignment:Splign:2.1.0"
     exon            309..431
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /inference="alignment:Splign:2.1.0"
     exon            432..554
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /inference="alignment:Splign:2.1.0"
     exon            555..596
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /inference="alignment:Splign:2.1.0"
     exon            597..671
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /inference="alignment:Splign:2.1.0"
     exon            672..1451
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1409..1414
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /note="hexamer: AATAAA"
     regulatory      1431..1436
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /note="hexamer: AATATA"
     polyA_site      1432
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
     polyA_site      1451
                     /gene="UBXN8"
                     /gene_synonym="D8S2298E; REP8; UBXD6"
                     /note="major polyA site"
ORIGIN      
        1 cgcctgcacc aggcgcttcc gccaccatgg cttcacgtgg ggttgttggc attttcttcc
       61 tctctgctgt cccccttgtg tgtctggagc tccggcgtgg gatcccggat ataggaatca
      121 aggattttct tttgctttgt ggccggattt tgctactgct tgctcttctt actttaacta
      181 tttctgtgac tacctcatgg cttaactcat ttaaatctcc ccaagtttat ctgaaggaag
      241 aagaagaaaa gaatgagaaa agacaaaaac ttgtgagaaa aaaacaacaa gaagcacaag
      301 gagagaaggc cagcagatac atagagaatg ttttaaaacc tcaccaggaa atgaaattga
      361 gaaaactgga ggagcgcttt tatcaaatga cgggtgaagc ctggaaatta agcagtggtc
      421 acaaacttgg gggtgatgaa ggtacaagtc agacatcttt tgaaacatca aacagagaag
      481 cagcaaagag ccagaacttg cctaaacctt taactgaatt tccgtctcct gctgaacagc
      541 ccacatgcaa ggagattcct gatttacctg aagaaccttc tcaaacagca gaagaagtag
      601 ttactgttgc tctccgatgt cccagtggga atgtcctgag gagaaggttt ttgaagtcct
      661 acagctcaca ggtcttattt gactggatga cgagaattgg gtaccacata tctctataca
      721 gcctttctac ttcctttccc agacggcctc tggcagtgga gggaggccag tcgctggagg
      781 acataggaat aactgtggac actgtactca tcctggagga gaaggagcag accaactagg
      841 aaagaaggga gagctccctg tttgcatgaa gtcagttatg ctatgacctt ctggcacaat
      901 aaaggcttca ctttcaaatc acactatacc ttgattgagc tcatggcagt aaactttgaa
      961 cattgatatc catgggaata ggattagaaa aggattgctt tctatatata ataatctgtg
     1021 gactgtgcca ttttacagtg taccaaatga gaatgaggtt gaaatgtatg cagtaaggta
     1081 ctcagtaatt aattggtatt ttttcccagc tgacatgatt tctcagtgtt agaaaacaaa
     1141 cccttagaac tttcctttct gcctcttcaa tccatcttac cacacaatat ttcatgattc
     1201 aaattcttca aagtcttata cgcaggaatg tttattctgc tgtatttctg tgaaattaaa
     1261 aacttggaag aagcttcaaa gctcttggag gctttaaagt tctttctgtt gggtgtgcat
     1321 tacagtttac ttaactgatg tttgcgattt atataatttt gccttgtatt aaatgttaca
     1381 aagttccaaa tgaatcagta ttttaaaaaa taaaactatg aaagcattaa aatataggtg
     1441 aatttttaaa a
//
