LOCUS       NM_014607               3771 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens UBX domain protein 4 (UBXN4), mRNA.
ACCESSION   NM_014607
VERSION     NM_014607.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3771)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 3771)
  AUTHORS   Trepte,P., Kruse,S., Kostova,S., Hoffmann,S., Buntru,A.,
            Tempelmeier,A., Secker,C., Diez,L., Schulz,A., Klockmeier,K.,
            Zenkner,M., Golusik,S., Rau,K., Schnoegl,S., Garner,C.C. and
            Wanker,E.E.
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology
            for quantitative mapping of protein-protein interactions in
            mammalian cells
  JOURNAL   Mol Syst Biol 14 (7), e8071 (2018)
   PUBMED   29997244
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3771)
  AUTHORS   Li,X., Bykhovskaya,Y., Haritunians,T., Siscovick,D., Aldave,A.,
            Szczotka-Flynn,L., Iyengar,S.K., Rotter,J.I., Taylor,K.D. and
            Rabinowitz,Y.S.
  TITLE     A genome-wide association study identifies a potential novel gene
            locus for keratoconus, one of the commonest causes for corneal
            transplantation in developed countries
  JOURNAL   Hum Mol Genet 21 (2), 421-429 (2012)
   PUBMED   21979947
REFERENCE   6  (bases 1 to 3771)
  AUTHORS   Christianson,J.C., Olzmann,J.A., Shaler,T.A., Sowa,M.E.,
            Bennett,E.J., Richter,C.M., Tyler,R.E., Greenblatt,E.J.,
            Harper,J.W. and Kopito,R.R.
  TITLE     Defining human ERAD networks through an integrative mapping
            strategy
  JOURNAL   Nat Cell Biol 14 (1), 93-105 (2011)
   PUBMED   22119785
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3771)
  AUTHORS   Lim,P.J., Danner,R., Liang,J., Doong,H., Harman,C., Srinivasan,D.,
            Rothenberg,C., Wang,H., Ye,Y., Fang,S. and Monteiro,M.J.
  TITLE     Ubiquilin and p97/VCP bind erasin, forming a complex involved in
            ERAD
  JOURNAL   J Cell Biol 187 (2), 201-217 (2009)
   PUBMED   19822669
  REMARK    GeneRIF: Knockdown of erasin, a platform for p97/VCP and ubiquilin
            binding, or knockdown of ubiquilin in human cells slowed
            degradation of two classical endoplasmic reticulum-associated
            protein degradation substrates.
REFERENCE   8  (bases 1 to 3771)
  AUTHORS   Alexandru,G., Graumann,J., Smith,G.T., Kolawa,N.J., Fang,R. and
            Deshaies,R.J.
  TITLE     UBXD7 binds multiple ubiquitin ligases and implicates p97 in
            HIF1alpha turnover
  JOURNAL   Cell 134 (5), 804-816 (2008)
   PUBMED   18775313
REFERENCE   9  (bases 1 to 3771)
  AUTHORS   Liang,J., Yin,C., Doong,H., Fang,S., Peterhoff,C., Nixon,R.A. and
            Monteiro,M.J.
  TITLE     Characterization of erasin (UBXD2): a new ER protein that promotes
            ER-associated protein degradation
  JOURNAL   J Cell Sci 119 (Pt 19), 4011-4024 (2006)
   PUBMED   16968747
  REMARK    GeneRIF: Erasin/UBXD2 may be involved in
            endoplasmic-reticulum-associated protein degradation and in
            Alzheimer's disease.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC011893.7.
            
            On Nov 23, 2018 this sequence version replaced NM_014607.3.
            
            Summary: UBXD2 is an integral membrane protein of the endoplasmic
            reticulum (ER) that binds valosin-containing protein (VCP; MIM
            601023) and promotes ER-associated protein degradation (ERAD)
            (Liang et al., 2006 [PubMed 16968747]).[supplied by OMIM, Mar
            2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC035594.2, SRR1660805.200758.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2154361 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000272638.14/ ENSP00000272638.9
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-157               AC011893.7         45874-46030
            158-260             AC011893.7         52286-52388
            261-289             AC011893.7         57558-57586
            290-408             AC011893.7         58178-58296
            409-583             AC011893.7         59536-59710
            584-677             AC011893.7         65837-65930
            678-732             AC011893.7         73788-73842
            733-897             AC011893.7         74590-74754
            898-1025            AC011893.7         76439-76566
            1026-1128           AC011893.7         80268-80370
            1129-1260           AC011893.7         82967-83098
            1261-1463           AC011893.7         84202-84404
            1464-3771           AC011893.7         86768-89075
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q21.3"
     gene            1..3771
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /note="UBX domain protein 4"
                     /db_xref="GeneID:23190"
                     /db_xref="HGNC:HGNC:14860"
                     /db_xref="MIM:611216"
     exon            1..157
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..1602
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /note="UBX domain-containing 1; UBX domain containing 2;
                     UBX domain-containing protein 2"
                     /codon_start=1
                     /product="UBX domain-containing protein 4"
                     /protein_id="NP_055422.1"
                     /db_xref="CCDS:CCDS42761.1"
                     /db_xref="GeneID:23190"
                     /db_xref="HGNC:HGNC:14860"
                     /db_xref="MIM:611216"
                     /translation="MLWFQGAIPAAIATAKRSGAVFVVFVAGDDEQSTQMAASWEDDK
                     VTEASSNSFVAIKIDTKSEACLQFSQIYPVVCVPSSFFIGDSGIPLEVIAGSVSADEL
                     VTRIHKVRQMHLLKSETSVANGSQSESSVSTPSASFEPNNTCENSQSRNAELCEIPPT
                     SDTKSDTATGGESAGHATSSQEPSGCSDQRPAEDLNIRVERLTKKLEERREEKRKEEE
                     QREIKKEIERRKTGKEMLDYKRKQEEELTKRMLEERNREKAEDRAARERIKQQIALDR
                     AERAARFAKTKEEVEAAKAAALLAKQAEMEVKRESYARERSTVARIQFRLPDGSSFTN
                     QFPSDAPLEEARQFAAQTVGNTYGNFSLATMFPRREFTKEDYKKKLLDLELAPSASVV
                     LLPAGRPTASIVHSSSGDIWTLLGTVLYPFLAIWRLISNFLFSNPPPTQTSVRVTSSE
                     PPNPASSSKSEKREPVRKRVLEKRGDDFKKEGKIYRLRTQDDGEDENNTWNGNSTQQM
                     "
     exon            158..260
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            261..289
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            290..408
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            409..583
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            584..677
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            678..732
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            733..897
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1025
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1128
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1260
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1463
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..3771
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3751..3756
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /note="hexamer: AATAAA"
     polyA_site      3771
                     /gene="UBXN4"
                     /gene_synonym="erasin; UBXD2; UBXDC1"
                     /note="major polyA site"
ORIGIN      
        1 gaagacggag ggcggagccg gcttcgggac tgcggagact acacaccgag cgagcgcctg
       61 ggcccgaagg gagcgatgct gtggttccag ggcgccattc cggccgccat cgcgacggcc
      121 aaaaggagcg gcgcggtctt cgtggtgttc gtggcaggtg atgatgaaca gtctacacag
      181 atggctgcaa gttgggaaga tgataaagtt acagaagcat cttcaaacag ttttgttgct
      241 attaaaatcg ataccaaaag tgaagcctgc ctacagtttt cacaaatcta tcctgtagtg
      301 tgtgttccat ccagtttctt tattggagac agtggaattc ccttggaagt aatagcagga
      361 agtgtttctg cagatgaact tgttacaaga attcacaagg tccgacagat gcatttgcta
      421 aaaagtgaaa catcagtagc aaatggcagt cagtcagaaa gttcagtgtc tactccatct
      481 gcgtcatttg aacctaacaa cacttgtgaa aactctcagt ccagaaatgc agagctttgt
      541 gagataccac ccacttctga tacaaagtca gatactgcaa caggaggaga aagtgcaggc
      601 catgccactt cctctcagga gcctagtgga tgctcagatc agagacctgc agaggacctc
      661 aacatccgag tggaaagact aacaaaaaaa cttgaagaaa ggagagaaga gaaaagaaaa
      721 gaggaagaac agagagaaat taagaaggaa attgagagga gaaaaactgg aaaagaaatg
      781 ttggattata aaagaaaaca agaagaagaa ttaacaaaaa gaatgctgga ggaaagaaac
      841 agagagaaag cagaagatag ggcagctcga gaacgtataa aacagcagat tgcattggac
      901 cgtgcagaga gagctgctcg ttttgcaaag acaaaggaag aagtagaggc tgccaaagct
      961 gctgccttgc tagcaaaaca ggcagaaatg gaagtcaaga gggaatctta tgcaagagaa
     1021 agaagcactg ttgcaagaat tcaattccgt cttcctgatg gttcttcctt tacaaatcag
     1081 ttcccttctg atgctcctct agaagaggca aggcagtttg ctgcacagac tgttggcaac
     1141 acttacggta atttttcgtt agcaaccatg tttcccagga gggaatttac caaagaagat
     1201 tataaaaaga agttactgga tttggaactt gccccaagcg cttcggtggt actgttgcca
     1261 gcaggaagac caactgcatc cattgtacac tcttccagcg gagacatttg gaccttgttg
     1321 ggaacagtgc tttatccatt ccttgccatc tggagattaa ttagcaattt cttgtttagt
     1381 aatccgcctc ccacacagac ttcagtgaga gtaacatcgt cagaaccccc aaaccctgca
     1441 tcatctagca aatcagaaaa aagggaacca gtgagaaaaa gagtgctgga aaaacgtgga
     1501 gacgacttta aaaaggaggg gaaaatttat agattaagga ctcaagatga tggtgaagat
     1561 gaaaacaaca cttggaatgg aaattccact caacagatgt agtgtgacaa gtataatatg
     1621 tgcaataatc attgtttctc ttatgattta attcaactaa aattctactg gagaagtggg
     1681 actgctttat attttccaac tggtctataa aatgtctctt tattcctgct tagtgggtgt
     1741 gggttgaagg tgtttaactc agaaaagtaa agacaggaaa taactctctg ctaggtcctt
     1801 gcttatatgg caaccactgc tagaacccta aaagaaccaa aaatctgcca cagcctgcct
     1861 ccatcagctt cttatttagt atttcatatg cccattagcc ctatgcttca gatgacacgt
     1921 tttgtttaga gctactttgc tccaagactc ttaagcccaa agtaactggt atgtcactga
     1981 gtaacttgac tcggtgtcag agcattttaa ctagccactc agatgagaat ttatgtttaa
     2041 cttctctttt tactcatcag ctgcaagcaa aatcttgtag tttttaatct taaacactga
     2101 ataaaaaaac tttcccctaa attggaatga tcttagtttt gctttgagtt ttgttatcta
     2161 gcatcttttt gttgcacagg gctctattga ggtcctatgt ctctgatttt tttttttccc
     2221 cagtattgcc ctggagctgt ctctggaaag tagctggcga ggttacctta actatcactg
     2281 aagaaagaaa ttttctgaca cactgatggc atgtgacttg tctcctaagt cagtgaggca
     2341 tcactttgtt tgcataaagt atacggtttg ttaaggcctt tgttcttgtg agatgcaaaa
     2401 cagctgctag tctgcaacct agttttccct ctcaccttta actgacgttt tgtcctcaat
     2461 aattacacaa ggacctagag tacctatagg acaaaaagta tagaataaaa atatgccttt
     2521 agtcatttgg tttttcttaa aaagttgaga ttcttaatct gacttacatg ttactttatc
     2581 cgtatgtctt tgttagtgga gaccgctaaa ctaatgatgt ttgaaaacag ttcctctgtt
     2641 ttagattgga agatagcact ctagagtgga catacggaaa gactgtgact ttattttgta
     2701 atgggaggaa gaaattttct cagagcaaac tttctatttt ttacctgtga aataacagtg
     2761 actttttaaa atggtgacag tgttggcaag gaaacagcaa cacaggctgc gctgttggta
     2821 ggagtgaaaa ccagtataat tcttctgaaa aacatttatc agaaacttaa aatatttcat
     2881 accgtttgat ccagtagctt cttctaaatc ataaatgcag acaatgttta ggtaaagaca
     2941 tactcattaa gtgttattta ttttactcaa gaactggaaa ccaactaaat gccttctata
     3001 gaagtaattt ttgatgagga gaaatggtac aatactaatt aacaacttgg tttaacatgt
     3061 ttactgagca tctgttaagt gttgggggaa aaagcagcag gatccagagc tataggtaca
     3121 gtgtgatctc agctttgcaa acacattttc tacatagata gtactaggta ttaatagata
     3181 tgtaaagaaa gaaatcacac cattaataat ggtaagattg gtttatgtga ttttagtggt
     3241 atttttggca cccttatata tgttttccaa actttcagca gtgatattat ttccataact
     3301 taaaaagtga gtttgaaaaa gaaaatctcc agcaagcatc tcatttaaat aaaggtttgt
     3361 catctttaaa aatacagcaa tatgtgactt tttaaaaaag ctgtcaaata ggtgtgaccc
     3421 tactaataat tattagaaat acatttaaaa acatcgagta cctcaagtca gtttgccttg
     3481 aaaaatatca aatataactc ttagagaaat gtacataaaa gaatgcttcg taattttgga
     3541 gtaggaggtt ccctcctcaa ttttgtattt ttaaaagtac atggtaaaaa aaaaaattca
     3601 caacagtata taaggctgta aaatgagaat tctgccccct cacctcttac cccagtacta
     3661 ttctccagag gtaatctatt aacaatttct tatgtaattt tcagaaaatt tgtatgcgta
     3721 tataagcaaa tatgtaatct ttatttttta aataaatggg atcatattat a
//
