LOCUS       NM_174916               7698 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens ubiquitin protein ligase E3 component n-recognin 1
            (UBR1), mRNA.
ACCESSION   NM_174916
VERSION     NM_174916.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Yuan,W., Han,J., Chen,C., Qiu,Y., Xu,Y., Huang,Y., Chen,Z., Xu,A.
            and Sun,M.
  TITLE     UBR1 is a prognostic biomarker and therapeutic target associated
            with immune cell infiltration in gastric cancer
  JOURNAL   Aging (Albany NY) 16 (16), 12029-12049 (2024)
   PUBMED   39181686
  REMARK    GeneRIF: UBR1 is a prognostic biomarker and therapeutic target
            associated with immune cell infiltration in gastric cancer.
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Zhao,W., Zhang,Y., Lin,S., Li,Y., Zhu,A.J., Shi,H. and Liu,M.
  TITLE     Identification of Ubr1 as an amino acid sensor of steatosis in
            liver and muscle
  JOURNAL   J Cachexia Sarcopenia Muscle 14 (3), 1454-1467 (2023)
   PUBMED   37057345
  REMARK    GeneRIF: Identification of Ubr1 as an amino acid sensor of
            steatosis in liver and muscle.
REFERENCE   3  (bases 1 to 7698)
  AUTHORS   Wang,B.B., Xu,H., Isenmann,S., Huang,C., Elorza-Vidal,X.,
            Rychkov,G.Y., Estevez,R., Schittenhelm,R.B., Lukacs,G.L. and
            Apaja,P.M.
  TITLE     Ubr1-induced selective endophagy/autophagy protects against the
            endosomal and Ca2+-induced proteostasis disease stress
  JOURNAL   Cell Mol Life Sci 79 (3), 167 (2022)
   PUBMED   35233680
  REMARK    GeneRIF: Ubr1-induced selective endophagy/autophagy protects
            against the endosomal and Ca(2+)-induced proteostasis disease
            stress.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7698)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 7698)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 7698)
  AUTHORS   Dgany,O., Avidan,N., Delaunay,J., Krasnov,T., Shalmon,L.,
            Shalev,H., Eidelitz-Markus,T., Kapelushnik,J., Cattan,D.,
            Pariente,A., Tulliez,M., Cretien,A., Schischmanoff,P.O.,
            Iolascon,A., Fibach,E., Koren,A., Rossler,J., Le Merrer,M.,
            Yaniv,I., Zaizov,R., Ben-Asher,E., Olender,T., Lancet,D.,
            Beckmann,J.S. and Tamary,H.
  TITLE     Congenital dyserythropoietic anemia type I is caused by mutations
            in codanin-1
  JOURNAL   Am J Hum Genet 71 (6), 1467-1474 (2002)
   PUBMED   12434312
REFERENCE   7  (bases 1 to 7698)
  AUTHORS   Kwon,Y.T., Reiss,Y., Fried,V.A., Hershko,A., Yoon,J.K., Gonda,D.K.,
            Sangan,P., Copeland,N.G., Jenkins,N.A. and Varshavsky,A.
  TITLE     The mouse and human genes encoding the recognition component of the
            N-end rule pathway
  JOURNAL   Proc Natl Acad Sci U S A 95 (14), 7898-7903 (1998)
   PUBMED   9653112
REFERENCE   8  (bases 1 to 7698)
  AUTHORS   Varshavsky,A.
  TITLE     The N-end rule: functions, mysteries, uses
  JOURNAL   Proc Natl Acad Sci U S A 93 (22), 12142-12149 (1996)
   PUBMED   8901547
  REMARK    Review article
REFERENCE   9  (bases 1 to 7698)
  AUTHORS   Chiannilkulchai,N., Pasturaud,P., Richard,I., Auffray,C. and
            Beckmann,J.S.
  TITLE     A primary expression map of the chromosome 15q15 region containing
            the recessive form of limb-girdle muscular dystrophy (LGMD2A) gene
  JOURNAL   Hum Mol Genet 4 (4), 717-725 (1995)
   PUBMED   7633422
REFERENCE   10 (bases 1 to 7698)
  AUTHORS   Shelton,C., LaRusch,J. and Whitcomb,D.C.
  TITLE     Pancreatitis Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   24624459
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BE873236.1, AY061886.1,
            AK127478.1 and BX648400.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_174916.2.
            
            Summary: The N-end rule pathway is one proteolytic pathway of the
            ubiquitin system. The recognition component of this pathway,
            encoded by this gene, binds to a destabilizing N-terminal residue
            of a substrate protein and participates in the formation of a
            substrate-linked multiubiquitin chain. This leads to the eventual
            degradation of the substrate protein. The protein described in this
            record has a RING-type zinc finger and a UBR-type zinc finger.
            Mutations in this gene have been associated with Johanson-Blizzard
            syndrome. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC113505.1, AY061886.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000290650.9/ ENSP00000290650.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                BE873236.1         51-66
            17-5266             AY061886.1         1-5250
            5267-5909           AK127478.1         1172-1814
            5910-6180           BX648400.1         2772-3042
            6181-6511           AK127478.1         2086-2416
            6512-7698           BX648400.1         3374-4560
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q15.2"
     gene            1..7698
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /note="ubiquitin protein ligase E3 component n-recognin 1"
                     /db_xref="GeneID:197131"
                     /db_xref="HGNC:HGNC:16808"
                     /db_xref="MIM:605981"
     exon            1..97
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..5266
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /EC_number="2.3.2.27"
                     /note="ubiquitin ligase E3 alpha-I; ubiquitin-protein
                     ligase E3-alpha; E3a ligase; N-recognin-1;
                     ubiquitin-protein ligase E3-alpha-1; ubiquitin-protein
                     ligase E3-alpha-I; RING-type E3 ubiquitin transferase
                     UBR1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase UBR1"
                     /protein_id="NP_777576.1"
                     /db_xref="CCDS:CCDS10091.1"
                     /db_xref="GeneID:197131"
                     /db_xref="HGNC:HGNC:16808"
                     /db_xref="MIM:605981"
                     /translation="MADEEAGGTERMEISAELPQTPQRLASWWDQQVDFYTAFLHHLA
                     QLVPEIYFAEMDPDLEKQEESVQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGRV
                     FKSGETTYSCRDCAIDPTCVLCMDCFQDSVHKNHRYKMHTSTGGGFCDCGDTEAWKTG
                     PFCVNHEPGRAGTIKENSRCPLNEEVIVQARKIFPSVIKYVVEMTIWEEEKELPPELQ
                     IREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAY
                     AACQEAKEDIKSHSENVSQHPLHVEVLHSEIMAHQKFALRLGSWMNKIMSYSSDFRQI
                     FCQACLREEPDSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEF
                     VKYYKQLQKEYISDDHDRSISITALSVQMFTVPTLARHLIEEQNVISVITETLLEVLP
                     EYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTIWTERLRMQFLEGFRSFLKI
                     LTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEELLLVAYKE
                     CHKAVMRCSTSFISSSKTVVQSCGHSLETKSYRVSEDLVSIHLPLSRTLAGLHVRLSR
                     LGAVSRLHEFVSFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVK
                     CREEMYDKDIIMLQIGASLMDPNKFLLLVLQRYELAEAFNKTISTKDQDLIKQYNTLI
                     EEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNET
                     GLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQ
                     ENKDEALPPPPPPEFCPAFSKVINLLNCDIMMYILRTVFERAIDTDSNLWTEGMLQMA
                     FHILALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNIQMLLEKLKGIPQLEGQK
                     DMITWILQMFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHR
                     QKIMAQMSALQKNFIETHKLMYDNTSEMPGKEDSIMEEESTPAVSDYSRIALGPKRGP
                     SVTEKEVLTCILCQEEQEVKIENNAMVLSACVQKSTALTQHRGKPIELSGEALDPLFM
                     DPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLC
                     NTVIPIIPLQPQKINSENADALAQLLTLARWIQTVLARISGYNIRHAKGENPIPIFFN
                     QGMGDSTLEFHSILSFGVESSIKYSNSIKEMVILFATTIYRIGLKVPPDERDPRVPML
                     TWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRITCPQVLIQKHL
                     VRLLSVVLPNIKSEDTPCLLSIDLFHVLVGAVLAFPSLYWDDPVDLQPSSVSSSYNHL
                     YLFHLITMAHMLQILLTVDTGLPLAQVQEDSEEAHSASSFFAEISQYTSGSIGCDIPG
                     WYLWVSLKNGITPYLRCAALFFHYLLGVTPPEELHTNSAEGEYSALCSYLSLPTNLFL
                     LFQEYWDTVRPLLQRWCADPALLNCLKQKNTVVRYPRKRNSLIELPDDYSCLLNQASH
                     FRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACIFHALHCGAGVCIFL
                     KIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHC
                     IIEEIARSQETNQMLFGFNWQLL"
     exon            98..354
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            355..433
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            434..544
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            545..675
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            676..814
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            815..877
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            878..1001
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1109
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1198
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1297
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1455
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1555
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1684
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1865
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1927
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2038
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2104
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2206
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2207..2270
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2271..2395
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2448
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2449..2551
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2600
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2755
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2756..2855
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2856..2956
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            2957..3043
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            3044..3225
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            3226..3431
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            3432..3525
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            3526..3675
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            3676..3773
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            3774..3864
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            3865..4013
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            4014..4069
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            4070..4166
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            4167..4234
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            4235..4385
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            4386..4473
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            4474..4607
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            4608..4716
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            4717..4773
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            4774..4851
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            4852..5022
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            5023..5124
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     exon            5125..7698
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5398..5403
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /note="hexamer: AATAAA"
     polyA_site      5418
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /note="major polyA site"
     regulatory      7671..7676
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /note="hexamer: AATATA"
     polyA_site      7698
                     /gene="UBR1"
                     /gene_synonym="JBS"
                     /note="major polyA site"
ORIGIN      
        1 gtcagtttcc ctcaagatgg cggacgagga ggctggaggt actgagagga tggaaatcag
       61 cgcggagtta ccccagaccc ctcagcgtct ggcatcttgg tgggatcagc aagttgattt
      121 ttatactgct ttcttgcatc atttggcaca attggtgcca gaaatttact ttgctgaaat
      181 ggacccagac ttggaaaagc aggaggaaag tgtacaaatg tcaatattca ctccactgga
      241 atggtactta tttggagaag atccagatat ttgcttagag aaattgaagc acagtggagc
      301 atttcagctt tgtgggaggg ttttcaaaag tggagagaca acctattctt gcagggattg
      361 tgcaattgat ccaacatgtg tactctgtat ggactgcttc caggacagtg ttcataaaaa
      421 tcatcgttac aagatgcata cttctactgg aggagggttc tgtgactgtg gagacacaga
      481 ggcatggaaa actggccctt tttgtgtaaa tcatgaacct ggaagagcag gtactataaa
      541 agagaattca cgctgtccgt tgaatgaaga ggtaattgtc caagccagga aaatatttcc
      601 ttcagtgata aaatatgtcg tagaaatgac tatatgggaa gaggaaaaag aactgcctcc
      661 tgaactccag ataagggaga aaaatgaaag atactattgt gtccttttca atgatgaaca
      721 ccattcatat gaccacgtca tatacagcct acaaagagct cttgactgtg agctcgcaga
      781 ggcccagttg cataccactg ccattgacaa agagggtcgt cgggctgtta aagcgggagc
      841 ttatgctgct tgccaggaag caaaggaaga tataaagagt cattcagaaa atgtctctca
      901 acatccactt catgtagaag tattacactc agagattatg gctcatcaga aatttgcttt
      961 gcgtcttggt tcctggatga acaaaattat gagctattca agtgacttta ggcagatctt
     1021 ttgccaagca tgccttagag aagaacctga ctcggagaat ccctgtctca taagcaggtt
     1081 aatgctttgg gatgcaaagc tttataaagg tgcccgtaag atccttcatg aattgatctt
     1141 cagcagtttt tttatggaga tggaatacaa aaaactcttt gctatggaat ttgtgaagta
     1201 ttataaacaa ctgcagaaag aatatatcag tgatgatcat gacagaagta tctctataac
     1261 tgcactttca gttcagatgt ttactgttcc tactctggct cgacatctta ttgaagagca
     1321 gaatgttatc tctgtcatta ctgaaactct gctagaagtt ttacctgagt acttggacag
     1381 gaacaataaa ttcaacttcc agggttatag ccaggacaaa ttgggaagag tatatgcagt
     1441 aatatgtgac ctaaagtata tcctgatcag caaacccaca atatggacag aaagattaag
     1501 aatgcagttc cttgaaggtt ttcgatcttt tttgaagatt cttacctgta tgcagggaat
     1561 ggaagaaatc cgaagacagg ttgggcaaca cattgaagtg gatcctgatt gggaggctgc
     1621 cattgctata cagatgcaat tgaagaatat tttactcatg ttccaagagt ggtgtgcttg
     1681 tgatgaagaa ctcttacttg tggcttataa agaatgtcac aaagctgtga tgaggtgcag
     1741 taccagtttc atatctagta gcaagacagt agtacaatcg tgtggacata gtttggaaac
     1801 aaagtcctac agagtatctg aggatcttgt aagcatacat ctgccactct ctaggaccct
     1861 tgctggtctt catgtacgtt taagcaggct gggtgctgtt tcaagactgc atgaatttgt
     1921 gtcttttgag gactttcaag tagaggtact agtggaatat cctttacgtt gtctggtgtt
     1981 ggttgcccag gttgttgctg agatgtggcg aagaaatgga ctgtctctta ttagccaggt
     2041 gttttattac caagatgtta agtgcagaga agaaatgtat gataaagata tcatcatgct
     2101 tcagattggt gcatctttaa tggatcccaa taagttcttg ttactggtac ttcagaggta
     2161 tgaacttgcc gaggctttta acaagaccat atctacaaaa gaccaggatt tgattaaaca
     2221 atataataca ctaatagaag aaatgcttca ggtcctcatc tatattgtgg gtgagcgtta
     2281 tgtacctgga gtgggaaatg tgaccaaaga agaggtcaca atgagagaaa tcattcactt
     2341 gctttgcatt gaacccatgc cacacagtgc cattgccaaa aatttacctg agaatgaaaa
     2401 taatgaaact ggcttagaga atgtcataaa caaagtggcc acatttaaga aaccaggtgt
     2461 atcaggccat ggagtttatg aactaaaaga tgaatcactg aaagacttca atatgtactt
     2521 ttatcattac tccaaaaccc agcatagcaa ggctgaacat atgcagaaga aaaggagaaa
     2581 acaagaaaac aaagatgaag cattgccgcc accaccacct cctgaattct gccctgcttt
     2641 cagcaaagtg attaaccttc tcaactgtga tatcatgatg tacattctca ggaccgtatt
     2701 tgagcgggca atagacacag attctaactt gtggaccgaa gggatgctcc aaatggcttt
     2761 tcatattctg gcattgggtt tactagaaga gaagcaacag cttcaaaaag ctcctgaaga
     2821 agaagtaaca tttgactttt atcataaggc ttcaagattg ggaagttcag ccatgaatat
     2881 acaaatgctt ttggaaaaac tcaaaggaat tccccagtta gaaggccaga aggacatgat
     2941 aacgtggata cttcagatgt ttgacacagt gaagcgatta agagaaaaat cttgtttaat
     3001 tgtagcaacc acatcaggat cggaatctat taagaatgat gagattactc atgataaaga
     3061 aaaagcagaa cgaaaaagaa aagctgaagc tgctaggcta catcgccaga agatcatggc
     3121 tcagatgtct gccttacaga aaaacttcat tgaaactcat aaactcatgt atgacaatac
     3181 atcagaaatg cctgggaaag aagattccat tatggaggaa gagagcaccc cagcagtcag
     3241 tgactactct agaattgctt tgggtcctaa acggggtcca tctgttactg aaaaggaggt
     3301 gctgacgtgc atcctttgcc aagaagaaca ggaggtgaaa atagaaaata atgccatggt
     3361 attatcggcc tgtgtccaga aatctactgc cttaacccag cacaggggaa aacccataga
     3421 actctcagga gaagccctag acccactttt catggatcca gacttggcat atggaactta
     3481 tacaggaagc tgtggtcatg taatgcacgc agtgtgctgg cagaagtatt ttgaagctgt
     3541 acagctgagc tctcagcagc gcattcatgt tgaccttttt gacttggaaa gtggagaata
     3601 tctttgccct ctttgcaaat ctctgtgcaa tactgtgatc cccattattc ctttgcaacc
     3661 tcaaaagata aacagtgaga atgcagatgc tcttgctcaa cttttgaccc tggcacggtg
     3721 gatacagact gttctggcca gaatatcagg ttataatata agacatgcta aaggagaaaa
     3781 cccaattcct attttcttta atcaaggaat gggagattct actttggagt tccattccat
     3841 cctgagtttt ggcgttgagt cttcgattaa atattcaaat agcatcaagg aaatggttat
     3901 tctctttgcc acaacaattt atagaattgg attgaaagtg ccacctgatg aaagggatcc
     3961 tcgagtcccc atgctgacct ggagcacctg cgctttcact atccaggcaa ttgaaaatct
     4021 attgggagat gaaggaaaac ctctgtttgg agcacttcaa aataggcagc ataatggtct
     4081 gaaagcatta atgcagtttg cagttgcaca gaggattacc tgtcctcagg tcctgataca
     4141 gaaacatctg gttcgtcttc tatcagttgt tcttcctaac ataaaatcag aagatacacc
     4201 atgccttctg tctatagatc tgtttcatgt tttggtgggt gctgtgttag cattcccatc
     4261 cttgtattgg gatgaccctg ttgatctgca gccttcttca gttagttctt cctataacca
     4321 cctttatctc ttccatttga tcaccatggc acacatgctt cagatactac ttacagtaga
     4381 cacaggccta ccccttgctc aggttcaaga agacagtgaa gaggctcatt ccgcatcttc
     4441 tttctttgca gaaatttctc aatatacaag tggctccatt gggtgtgata ttcctggctg
     4501 gtatttgtgg gtctcactga agaatggcat caccccttat cttcgctgtg ctgcattgtt
     4561 tttccactat ttacttgggg taactccgcc tgaggaactg cataccaatt ctgcagaagg
     4621 agagtacagt gcactctgta gctatctatc tttacctaca aatttgttcc tgctcttcca
     4681 ggaatattgg gatactgtaa ggcccttgct ccagaggtgg tgtgcagatc ctgccttact
     4741 aaactgtttg aagcaaaaaa acaccgtggt caggtaccct agaaaaagaa atagtttgat
     4801 agagcttcct gatgactata gctgcctcct gaatcaagct tctcatttca ggtgcccacg
     4861 gtctgcagat gatgagcgaa agcatcctgt cctctgcctt ttctgtgggg ctatactatg
     4921 ttctcagaac atttgctgcc aggaaattgt gaacggggaa gaggttggag cttgcatttt
     4981 tcacgcactt cactgtggag ccggagtctg cattttccta aaaatcagag aatgccgagt
     5041 ggtcctggtt gaaggtaaag ccagaggctg tgcctatcca gctccttact tggatgaata
     5101 tggagaaaca gaccctggcc tgaagagggg caaccccctt catttatctc gtgagcggta
     5161 tcggaagctc catttggtct ggcaacaaca ctgcattata gaagagattg ctaggagcca
     5221 agagactaat cagatgttat ttggattcaa ctggcagtta ctgtgagctc caactctgcc
     5281 tcaagacaat cacaaatgac gacagtagta aaggctgatt caaaattatg gaaaactttc
     5341 tgagggctgg gaaagtattg gagggtcttt tgctccatgt ccaggttcac ttacatcaat
     5401 aaaatatttc ttaatggagt attgctttca attagcaaac atatgcttca caggaaaaaa
     5461 ggacatagat caatctgttt tatgtgctag tatttccagg aatttattcc ccttcataat
     5521 ttgtctcatt tcattttatt tcatccactt ggtagatgaa gtcacgtcaa acagttgtag
     5581 acattttata tgttggttaa ctcttctgca attttgtatt tggtgttttc cccccaagtt
     5641 tagttcaact gacattggat cactgacaaa attctaataa tctgtgatag tcttccttgc
     5701 agttaaagaa gaattgcaga aaccatgcaa tatacttggg aaagattcca aaaataaatt
     5761 ttttattatt tctcttttaa ggaaataccc ctaatgtgcc acctgctgct atcaccacaa
     5821 attaaactca atctctatgt ggacagagga tgatttctgc caatatggaa aagctttttt
     5881 ctcactgtag gcctcaagaa aagttagggt aatgtatttg ttattcattc ctgacggtac
     5941 aaagagcttg cagttctcac ctctgactac cagtagcttt gttgagtttt gaaataatac
     6001 ttgacatttt ccaaaggcaa atctcattct gcaaggagat tgtggcacca tcctgtttga
     6061 ctctcagaaa cctcttgtaa ttctgatgta aaaactgtag aatgaagatg agaaaattct
     6121 cgcaatgagt ggatcatgac aactgtaaat tagaacaatc agatttaaac caattccgca
     6181 gtcttctata tctttgtaaa agacaaatcc ttgatgttgt ctgtgtgcaa ccttttcata
     6241 aactctggtt ttatgactag tacaaaccac caaaaaagcc atgtgatcaa tagtctgtgt
     6301 cctgttataa catgctgtgg ttgagccatc ttgtttataa ataatagagc tctcctgaat
     6361 ttgtgcatag acttcttggt tcctggcttt tgttttttgt atcaagagat tgtgatataa
     6421 aacagcagaa gataaatgga aaccttccat tttaacttac gttgtttctg gggtaatgtt
     6481 agaaccttga aagatgcatt caaagactgt atcttatttt gcccttggct attagtgtct
     6541 cacatatgtg tgtaaatgtt ttcctacctt ctttttgctc agcaaaggca agcaagtaaa
     6601 atatatttgc taagtgatta gtgatgcaca tttggggcta gatttttttg gtacttttat
     6661 gtaaagaaaa gtggattttg cagtaaggga ttggcatgag caggcgtcag aatcacaatc
     6721 atgattttct acttgaataa ttacaattca gaaggtatct ggataaatag atacatgtct
     6781 agtgaacaat ttgtaacaat aacaggtaag gatcaggaaa ttcagtattc agtttgtcag
     6841 atttgccaga atgatgaaag tatttgaaca tgtgtgtttg tttcttatat aattgtattg
     6901 agtggattgt ttgactggga aatctgggct agaataggaa acagaagata ctgacttcta
     6961 ccctaataga tgggccccaa tttagcaaag ataaactgac tttattttta gtccttttta
     7021 tattaactta ataaattctg gagttaggct ctcaagagga cagagggact gtctggcaat
     7081 ggccagccag acctttactg ccaaagaacc catttcatat tgcgttccac tgattgagat
     7141 tgattcagat ttttgcactg tagatgagcg tatgtctcag tgctgcccca agccccaggg
     7201 atttctcatt atgttcaaat gtcctagtga tttaccttaa tcattgcaaa caattatgct
     7261 tatgaagttt acttacaaac aagcaactga gtcactttat tttctttagt gtagtatgtg
     7321 aaggcactgg ttcaacagga tggctccaga actgtgtttt tctaatgttt ggtaaggggc
     7381 tagtgagaat tttaatgata tggtgaagaa aaatatatct gtataattaa tttattatat
     7441 tggtgtatgg gctgtgagtt caccttttag tggtcatttg tcatttcata acaactatgc
     7501 attttggttc actgtgatga tgatctatat ttagtgactg caacatgttt ataccactga
     7561 ttcaaattcc atccatgatg aagttataca aataatgcat atattgataa cttttattgc
     7621 aaaaatgtaa atttaaaact tgtataatgt tcttgtgctt tttaaaataa aatatatgtg
     7681 tatatttaaa aagaaaaa
//
