LOCUS       NM_001079514            6443 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ubinuclein 1 (UBN1), transcript variant 2, mRNA.
ACCESSION   NM_001079514
VERSION     NM_001079514.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Jaiswal,A., Roy,R., Tamrakar,A., Singh,A.K., Kar,P. and Kodgire,P.
  TITLE     Activation-induced cytidine deaminase an antibody diversification
            enzyme interacts with chromatin modifier UBN1 in B-cells
  JOURNAL   Sci Rep 13 (1), 19615 (2023)
   PUBMED   37949972
  REMARK    GeneRIF: Activation-induced cytidine deaminase an antibody
            diversification enzyme interacts with chromatin modifier UBN1 in
            B-cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 6443)
  AUTHORS   Ricketts,M.D., Dasgupta,N., Fan,J., Han,J., Gerace,M., Tang,Y.,
            Black,B.E., Adams,P.D. and Marmorstein,R.
  TITLE     The HIRA histone chaperone complex subunit UBN1 harbors H3/H4- and
            DNA-binding activity
  JOURNAL   J Biol Chem 294 (23), 9239-9259 (2019)
   PUBMED   31040182
  REMARK    GeneRIF: UBN1 may be capable of binding two H3.3/H4 molecules to
            facilitate (H3.3/H4)2 tetramer formation in a pathway to nucleosome
            deposition that may also involve UBN1-mediated DNA binding by the
            HIRA complex.
REFERENCE   4  (bases 1 to 6443)
  AUTHORS   Ray-Gallet,D., Ricketts,M.D., Sato,Y., Gupta,K., Boyarchuk,E.,
            Senda,T., Marmorstein,R. and Almouzni,G.
  TITLE     Functional activity of the H3.3 histone chaperone complex HIRA
            requires trimerization of the HIRA subunit
  JOURNAL   Nat Commun 9 (1), 3103 (2018)
   PUBMED   30082790
  REMARK    GeneRIF: analysis of the trimeric HIRA, UBN1 and CABIN1 H3.3
            histone chaperone complex
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6443)
  AUTHORS   Ricketts,M.D. and Marmorstein,R.
  TITLE     A Molecular Prospective for HIRA Complex Assembly and H3.3-Specific
            Histone Chaperone Function
  JOURNAL   J Mol Biol 429 (13), 1924-1933 (2017)
   PUBMED   27871933
  REMARK    Review article
REFERENCE   6  (bases 1 to 6443)
  AUTHORS   Rai,T.S., Puri,A., McBryan,T., Hoffman,J., Tang,Y.,
            Pchelintsev,N.A., van Tuyn,J., Marmorstein,R., Schultz,D.C. and
            Adams,P.D.
  TITLE     Human CABIN1 is a functional member of the human HIRA/UBN1/ASF1a
            histone H3.3 chaperone complex
  JOURNAL   Mol Cell Biol 31 (19), 4107-4118 (2011)
   PUBMED   21807893
  REMARK    GeneRIF: Data show that, like HIRA, UBN1, and ASF1a, CABIN1 is
            involved in heterochromatinization of the genome of senescent human
            cells.
REFERENCE   7  (bases 1 to 6443)
  AUTHORS   Gruffat,H., Lupo,J., Morand,P., Boyer,V. and Manet,E.
  TITLE     The nuclear and adherent junction complex component protein
            ubinuclein negatively regulates the productive cycle of
            Epstein-Barr virus in epithelial cells
  JOURNAL   J Virol 85 (2), 784-794 (2011)
   PUBMED   21084479
  REMARK    GeneRIF: By a chromatin immunoprecipitation (ChIP) assay, the
            authors show that Ubn-1 blocks EB1-human herpesvirus 4 DNA
            interaction.
REFERENCE   8  (bases 1 to 6443)
  AUTHORS   Aho,S., Lupo,J., Coly,P.A., Sabine,A., Castellazzi,M., Morand,P.,
            Sergeant,A., Manet,E., Boyer,V. and Gruffat,H.
  TITLE     Characterization of the ubinuclein protein as a new member of the
            nuclear and adhesion complex components (NACos)
  JOURNAL   Biol Cell 101 (6), 319-334 (2009)
   PUBMED   18823282
  REMARK    GeneRIF: Analyses have revealed that in most cells ubinuclein
            occurred in the nucleoplasm, but in cells forming tight junctions
            it is recruited to the plaque structure of the zonula occludens.
REFERENCE   9  (bases 1 to 6443)
  AUTHORS   Banumathy,G., Somaiah,N., Zhang,R., Tang,Y., Hoffmann,J.,
            Andrake,M., Ceulemans,H., Schultz,D., Marmorstein,R. and Adams,P.D.
  TITLE     Human UBN1 is an ortholog of yeast Hpc2p and has an essential role
            in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells
  JOURNAL   Mol Cell Biol 29 (3), 758-770 (2009)
   PUBMED   19029251
  REMARK    GeneRIF: Hpc2-related domain of UBN1, UBN2, and Hpc2p is an
            evolutionarily conserved HIRA/Hir-binding domain, which directly
            interacts with the N-terminal WD repeats of HIRA/Hir.
REFERENCE   10 (bases 1 to 6443)
  AUTHORS   Aho,S., Buisson,M., Pajunen,T., Ryoo,Y.W., Giot,J.F., Gruffat,H.,
            Sergeant,A. and Uitto,J.
  TITLE     Ubinuclein, a novel nuclear protein interacting with cellular and
            viral transcription factors
  JOURNAL   J Cell Biol 148 (6), 1165-1176 (2000)
   PUBMED   10725330
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC020663.1 and AC027687.7.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001079514.2.
            
            Summary: Cellular senescence is a hallmark of tumor suppression and
            tissue aging. Senescent cells contain domains of heterochromatin,
            called senescence-associated heterochromatin foci (SAHF), that
            repress proliferation-promoting genes. The protein encoded by this
            gene binds to proliferation-promoting genes and is required for
            SAHF formation, enhancing methylation of histone H3. [provided by
            RefSeq, Oct 2016].
            
            Transcript Variant: This variant (2) represents the longest
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF108460.1, SRR7346977.2899130.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262376.11/ ENSP00000262376.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-730               AC020663.1         140242-140971
            731-1018            AC020663.1         145640-145927
            1019-1105           AC020663.1         150751-150837
            1106-1201           AC020663.1         151329-151424
            1202-1336           AC020663.1         151786-151920
            1337-1440           AC020663.1         152626-152729
            1441-1879           AC020663.1         153425-153863
            1880-1950           AC027687.7         61414-61484
            1951-2080           AC027687.7         62793-62922
            2081-2199           AC027687.7         63097-63215
            2200-2328           AC027687.7         63425-63553
            2329-2475           AC027687.7         63736-63882
            2476-2526           AC027687.7         65465-65515
            2527-2569           AC027687.7         65612-65654
            2570-3793           AC027687.7         66792-68015
            3794-4034           AC027687.7         69452-69692
            4035-4124           AC027687.7         69966-70055
            4125-6443           AC027687.7         72664-74982
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..6443
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /note="ubinuclein 1"
                     /db_xref="GeneID:29855"
                     /db_xref="HGNC:HGNC:12506"
                     /db_xref="MIM:609771"
     exon            1..730
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            731..1018
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             770..4174
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /note="isoform a is encoded by transcript variant 2;
                     HIRA-binding protein; ubiquitously expressed nuclear
                     protein"
                     /codon_start=1
                     /product="ubinuclein-1 isoform a"
                     /protein_id="NP_001072982.1"
                     /db_xref="CCDS:CCDS10525.1"
                     /db_xref="GeneID:29855"
                     /db_xref="HGNC:HGNC:12506"
                     /db_xref="MIM:609771"
                     /translation="MSEPHRVQFTSLPGSLNPAFLKKSRKEEAGAGEQHQDCEPAAAA
                     VRITLTLFEPDHKRCPEFFYPELVKNIRGKVKGLQPGDKKKDLSDPFNDEEKERHKVE
                     ALARKFEEKYGGKKRRKDRIQDLIDMGYGYDESDSFIDNSEAYDELVPASLTTKYGGF
                     YINSGTLQFRQASESEDDFIKEKKKKSPKKRKLKEGGEKIKKKKKDDTYDKEKKSKKS
                     KFSKAGFTALNASKEKKKKKYSGALSVKEMLKKFQKEKEAQKKREEEHKPVAVPSAEA
                     QGLRELEGASDPLLSLFGSTSDNDLLQAATAMDSLTDLDLEHLLSESPEGSPFRDMDD
                     GSDSLGVGLDQEFRQPSSLPEGLPAPLEKRVKELAQAARAAEGESRQKFFTQDINGIL
                     LDIEAQTRELSSQVRSGVYAYLASFLPCSKDALLKRARKLHLYEQGGRLKEPLQKLKE
                     AIGRAMPEQMAKYQDECQAHTQAKVAKMLEEEKDKEQRDRICSDEEEDEEKGGRRIMG
                     PRKKFQWNDEIRELLCQVVKIKLESQDLERNNKAQAWEDCVKGFLDAEVKPLWPKGWM
                     QARTLFKESRRGHGHLTSILAKKKVMAPSKIKVKESSTKPDKKVSVPSGQIGGPIALP
                     SDHQTGGLSIGASSRELPSQASGGLANPPPVNLEDSLDEDLIRNPASSVEAVSKELAA
                     LNSRAAGNSEFTLPAPSKAPAEKVGGVLCTEEKRNFAKPSPSAPPPASSLQSPLNFLA
                     EQALALGQSSQEKKPESSGYKELSCQAPLNKGLPEVHQSKAKHHSLPRTSHGPQVAVP
                     VPGPQVKVFHAGTQQQKNFTPPSPFANKLQGPKASPTQCHRSLLQLVKTAAKGQGFHP
                     SAPATSGGLSASSSSSHKTPASSSSALSHPAKPHSVSSAGSSYKNNPFASSISKHGVS
                     SGSSSSGGTPVQSSVSGSLVPGIQPPSVGQATSRPVPSSAGKKMPVSQKLTLVAPPGG
                     PNGDSSGGTQGVAKLLTSPSLKPSAVSSVTSSTSLSKGASGTVLLAGSSLMASPYKSS
                     SPKLSGAMSSNSLGIITPVPIPVHVLSFSADSSAKAGVSKDAIVTGPAPGSFHHGLGH
                     SLLAGLHSSPPHAAPLPHAAVPTHIPQSLPGASQLHGKGPAVPRKL"
     exon            1019..1105
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1201
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1336
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1440
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1879
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..1950
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2080
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2199
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2328
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2329..2475
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2476..2526
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2527..2569
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2570..3793
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3794..4034
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            4035..4124
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     exon            4125..6443
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6377..6382
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /note="hexamer: AATAAA"
     polyA_site      6402
                     /gene="UBN1"
                     /gene_synonym="VT; VT4"
                     /note="major polyA site"
ORIGIN      
        1 ggctccttcc ccctcccttc ggctcgtgac aacgaagcgc ccgcggtctg aggcggcggc
       61 ggcggcgacg gtgcgaccgg ctgagcgcga gagggagccg gcctcgcggc tcgccccgcc
      121 cccgggtctt ggactccgcg cccctcccct ctcggcgctt ccgttacgcc cgttggtccg
      181 gcgcgggccc cggggccatt cccgagcccg aggcgctggt cggccccgtc gcagcgccag
      241 tgagctaccc tgacggagct cggcgcggcc tggaactcag cctccgccgg gtctgggctc
      301 ccgcgcccgg caactctgct tggccgcagg ccgggaggcc ccggtgtccg cgcccgtccg
      361 ctcccgccgc ccgtgttctt tggagcgctc ctcccgacgg ccgctttcgg aggcgccgtc
      421 tgagcgcggg gtcccgcgcc gcaagttctc ctggggcgac cggaggctgc tcccccgacc
      481 ccctggtgtc cccggagtgg ctgcgcggac gtcgagttgg catttcttcg cttcctcctg
      541 ggccggcgcg cggcgcggca caccaggctc ccctgggctc ggggacccgg ccatgggccg
      601 aggcgcgggc cgcccgcccg ctgggagcca cggcttagca gccgaccgct agctgcgccg
      661 ccgcccgggg accggcatga ggaccgccgc ggggggacgt ctgcggcccg cgtcggcgct
      721 ggggacaaag aagccatgca gtgacacccg ctaagacttg ttggtagcca tgtcggagcc
      781 ccacagggtc cagttcacct ctctcccagg ttccctgaat cctgcgtttt tgaagaagtc
      841 ccggaaggag gaggctgggg caggagaaca gcatcaggac tgtgagccgg ctgcagcagc
      901 tgttcggatt acactcaccc tctttgaacc agatcacaaa cgctgcccag agttcttcta
      961 cccagagctg gtgaagaata tccgagggaa ggtaaaaggc cttcagcctg gagataagaa
     1021 gaaagatctg tcagatcctt tcaatgacga agaaaaggaa aggcataaag tagaggccct
     1081 tgcccgaaaa tttgaagaaa aatacggtgg aaagaaacgt agaaaagacc gaatacagga
     1141 cttgatcgat atggggtatg gttatgatga atccgactcc ttcatcgata actctgaggc
     1201 gtatgatgag cttgttcctg cttctttgac tacgaagtat ggaggatttt acattaactc
     1261 gggaaccctg cagtttagac aagcatcaga gtctgaagat gacttcatta aagaaaagaa
     1321 gaaaaaatct ccaaagaagc ggaagttgaa ggaaggtggt gagaagataa agaagaagaa
     1381 aaaagatgac acttatgaca aggagaagaa atcgaaaaag tccaagtttt ccaaagccgg
     1441 cttcacagcc ctcaatgcca gtaaggagaa gaagaagaag aaatattctg gggctttaag
     1501 cgttaaagag atgctaaaga aatttcagaa agagaaagag gctcagaaaa aaagggagga
     1561 ggagcataag cctgttgcgg tcccatcagc ggaagctcag ggcctgcggg aactggaggg
     1621 tgcctctgac cccttgctct cactctttgg ctctacttct gacaacgact tgctccaggc
     1681 ggccactgcc atggactcgc tgacggattt ggacttggag catctgctca gtgagtctcc
     1741 agaaggaagt cccttccgag atatggatga tggaagtgat tcccttgggg tgggattgga
     1801 ccaggaattc aggcagccct cttctctccc cgaaggcctg ccagcacccc tggagaagcg
     1861 cgttaaggag ctggctcagg ctgccagagc tgctgagggg gagagcagac agaagttctt
     1921 cacccaggat attaatggaa tcctattaga catagaggcg cagactcggg agctgagcag
     1981 tcaggtccgc tctggggtgt atgcctatct tgcgtcattc ctgccctgca gcaaggatgc
     2041 cctgctcaag cgtgctcgga aacttcacct ctatgaacag gggggccgtc tgaaggagcc
     2101 tctccagaag ctcaaggaag ccattggcag ggcgatgcca gagcagatgg ccaagtacca
     2161 ggacgaatgc caggcacaca cgcaggcaaa ggttgccaag atgctggaag aggagaaaga
     2221 caaggagcag agggaccgga tttgttcgga tgaggaagaa gatgaagaaa aagggggcag
     2281 gaggataatg ggacctcgga agaagttcca atggaatgat gagatcaggg agctactgtg
     2341 ccaggtggtg aagatcaaac tggagagcca ggacctggag aggaacaaca aagcccaggc
     2401 ttgggaggac tgtgtgaagg gctttctgga tgcggaagtc aagcccctct ggcccaaagg
     2461 ctggatgcag gccagaactc tgtttaagga gagcagacga ggccatgggc acctgacttc
     2521 aatcctggcc aagaagaaag ttatggcccc ttctaaaatc aaggtgaagg aatcgtctac
     2581 gaagcctgat aaaaaggttt ctgtcccatc aggacagatt ggtggcccca ttgctttgcc
     2641 ctcagatcac caaacaggag gcctgagtat tggggcctcg agcagggagc tcccatccca
     2701 ggcatcgggc ggccttgcta accctcctcc tgtcaacctg gaggactcat tggatgaaga
     2761 cttgatccgc aatccagcct cctcggtgga agccgtgtcc aaggaattgg ctgcattgaa
     2821 tagcagagca gctgggaact ctgaattcac actgcctgca ccctcaaaag cacctgcaga
     2881 aaaagttgga ggcgttttat gtacagaaga aaaaaggaac tttgcgaagc ctagtccttc
     2941 tgctccacca ccagctagct ctctgcagtc acccctcaat tttctggcag aacaggctct
     3001 ggcactgggg cagtcctctc aggagaaaaa accagagagt tctggctaca aagagctgtc
     3061 ctgccaggct cccctcaata agggcctgcc agaagtacat cagtccaaag ctaagcacca
     3121 cagcttgcca cggacgtctc acgggcccca agtggcagtt cctgtgcctg gcccccaggt
     3181 caaagtcttt catgccggca ctcagcagca gaaaaacttc acgcccccat ctccatttgc
     3241 caataagctc caaggcccaa aggcttctcc cacacagtgt catcgttccc tcctgcagtt
     3301 agtgaagaca gcggccaaag gccagggctt ccatccctct gcaccagcca cctcaggagg
     3361 cctgtcagcc tccagcagca gctctcacaa gaccccagcc tcgtcctctt ctgccctgag
     3421 ccatccagca aagccacatt cagtcagctc tgcaggctca tcttacaaga ataatccctt
     3481 tgccagctca atctccaaac atggggtttc ttctggcagc tcttcctcgg gaggaacacc
     3541 agtccagagt tctgtttctg ggagcctggt ccctggcata cagcctccct ccgtgggaca
     3601 ggccaccagc cgacccgtcc caagttcagc agggaaaaaa atgcctgttt cccagaagtt
     3661 gactctggta gcccctccag gcggtccaaa cggagattcc agtggtggga cccagggagt
     3721 ggcaaagttg ctgacctcgc cgtccctaaa gccctctgca gttagtagtg tgacatcgtc
     3781 tacctccttg tcaaaaggag cgagtgggac tgtgctgctg gccggctcct ctttgatggc
     3841 ttcaccctac aaatccagca gcccaaagct gtctggggcc atgagctcga actccttggg
     3901 aattataacc cctgtcccta ttcctgtcca tgtgctctcc ttcagcgctg actcctctgc
     3961 caaagcaggg gtctccaagg atgccatcgt cacaggccct gcccccgggt ccttccacca
     4021 tggccttggc cacagtcttc tggctggctt gcactccagc ccgccccatg cagcgcctct
     4081 cccacacgct gcggtgccca cccatatccc gcagagtctg ccaggtgctt ctcagcttca
     4141 cgggaaaggg cctgctgtac cacggaaatt gtgaccgctt cagaggcaag gcttgccact
     4201 tgggtctggg tggaatcaga acgtgcaggt ctcccaggat gtacactcac tgcgcccttt
     4261 ctgctgcttg gtgttcttct ggaggagcgt gagttctcag cggagcgctt ctcggcactt
     4321 ctgatgtgcc tcccatggag ggagccgggc ccttgctgct caggaggtgc agactgcccc
     4381 gtgctctggg ccttgcagct ctgtcgctag acggttgtta gaggggcagc tctaggctgg
     4441 ggcttgcgct gggccgtggt gggaggcaca gtgtttacag gctctggtgg cagagcagtt
     4501 ggcacacctg tgggtgaatc tgcctgatcc cctggcattt ggtcagagta cctcagagca
     4561 ccccactgct caggggctcc ttctggctgc agtaagctcc ctggatggtc acagtgccgc
     4621 cccatcccca ggctgtgtgc tcaaagcgga caaaactcag gccagagcca cagctgggag
     4681 acctgcactg tccctgcgaa atactaagaa cacctagggt gtgctcactg tgggggccag
     4741 tttctcctcg gaacatgaca atgaagctct tttagagaaa agacctttgt agattcaaca
     4801 attatgatag gatttttaca gacacctatt ttgggctcag ttttcatcat taccattaaa
     4861 tgcattggat agaaggggac tgttcttcac acatcatatt ataggaagac ataattccag
     4921 tgcctttatg gtggagcaga attcgacaac acatgtccat tcagccctct gaatggatct
     4981 gagacgaaga cctttttaaa gatatgtctg tattgaagag aaagccagtt ttgagttttt
     5041 tacacttgca catcattatc tgtgccgtcc tgactagaag gttgtctggg cccccaaatt
     5101 ccaagtccca gtatagccag ggctcccatt ttgtttttcc agagttcgtg gaggtggact
     5161 gcagagccca gagcccttca tctctctgag ggtcaaggat accaggggtc ccaagtcaca
     5221 gatagacatt ccagtttgta ttcttaggaa tcattcttag gcagtgcggc agaagcaggc
     5281 ctctgtgaag tgtctgccgg gtagaaatag gccttgaagt cctggggctc tccaggcagt
     5341 ggggttatgt gtcgactgaa gggtgatgtg cagaagagct tggagagtgg atcagagaga
     5401 ctaggacagt tactgcagag ctcctaagtc accactcaac cagtggctgt ttcacaagga
     5461 tttgagggca cttgcctgtc ttgtgaggtc tgtgccacaa ggtgagtctg aactgactgc
     5521 tccagctaag tgatcaggca cgttgagagt atacggtgtc ctcagcacat acacctgtgg
     5581 gaaaacggac cgctcttggc tcctgactcc cagggtacag cgcccagtaa ctcatccttc
     5641 attcagaggt ttgctggcct tcccggtttc ccaaggagca agacctctgt ccgccctttg
     5701 gcccggcctc tgctctgtct ccttttttta cataagaacc aatccgtgtt tcacattggg
     5761 ctaagtggat tccttatccc tttgttatat atattttttg caacttggat ttccagtttg
     5821 tttacagagt agtcttaggt agtagcaaaa gagccaaaga agagatttgt attgctgagc
     5881 tcaaagccaa gcagaggccg gcagtggagg ctgagcaggg actctccagg cttctctgcc
     5941 cataaacacc cgggtcccag gccctccttc ctttcccttt ggtgctccct cctccccacg
     6001 taccattgcg cgtgcagctg gagcagaata ccctcatttt taggaatcta gtgatgcctc
     6061 ttgcctcagg gaaacgtttc tttgatgggg agtttgagat ttcttttcct tgtttatgct
     6121 ttatttgtgg taatgaaaga gcgaatgact gaacagccat ggcaaggcag acctacaggc
     6181 gaggccgcag cagagggtgg ggcagagcag cctcggggtc tgggggctgc aggggtttcc
     6241 ctggcgcagc gaaagtctct gagcacttac cgggcgtgac cgtttcttag gtgtgagagg
     6301 ggctgtggct tttgtgcagc gactatgttg gtgttagggg tggtgtggag attgttaatc
     6361 ttgtataaag caattcaata aattgtttca aggtttccaa aacatttttt ctcactcttt
     6421 tctttctacc atgtggaagt aag
//
