LOCUS       NM_001105562            5905 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens ubiquitination factor E4B (UBE4B), transcript variant
            1, mRNA.
ACCESSION   NM_001105562
VERSION     NM_001105562.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Kim,H., Jung,S.Y., Yun,H.H., Yoo,K., Lee,J.S. and Lee,J.H.
  TITLE     UBE4B regulates p27 expression in A549 NSCLC cells through
            regulating the interaction of HuR and the p27 5' UTR
  JOURNAL   Biochem Biophys Res Commun 695, 149484 (2024)
   PUBMED   38211530
  REMARK    GeneRIF: UBE4B regulates p27 expression in A549 NSCLC cells through
            regulating the interaction of HuR and the p27 5' UTR.
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Le Clorennec,C., Subramonian,D., Huo,Y. and Zage,P.E.
  TITLE     UBE4B interacts with the ITCH E3 ubiquitin ligase to induce Ku70
            and c-FLIPL polyubiquitination and enhanced neuroblastoma apoptosis
  JOURNAL   Cell Death Dis 14 (11), 739 (2023)
   PUBMED   37957138
  REMARK    GeneRIF: UBE4B interacts with the ITCH E3 ubiquitin ligase to
            induce Ku70 and c-FLIPL polyubiquitination and enhanced
            neuroblastoma apoptosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5905)
  AUTHORS   Lyu,Y., Tan,B., Li,L., Liang,R., Lei,K., Wang,K., Wu,D., Lin,H. and
            Wang,M.
  TITLE     A novel protein encoded by circUBE4B promotes progression of
            esophageal squamous cell carcinoma by augmenting MAPK/ERK signaling
  JOURNAL   Cell Death Dis 14 (6), 346 (2023)
   PUBMED   37264022
  REMARK    GeneRIF: A novel protein encoded by circUBE4B promotes progression
            of esophageal squamous cell carcinoma by augmenting MAPK/ERK
            signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5905)
  AUTHORS   Shao,X., Zhu,J., Shi,Y., Fang,H., Chen,J., Zhang,Y., Wang,J.,
            Jian,H., Lan,S., Jiang,F., Zhong,F., Zhang,Y. and Cao,C.
  TITLE     Upregulated UBE4B expression correlates with poor prognosis and
            tumor immune infiltration in hepatocellular carcinoma
  JOURNAL   Aging (Albany NY) 14 (23), 9632-9646 (2022)
   PUBMED   36470669
  REMARK    GeneRIF: Upregulated UBE4B expression correlates with poor
            prognosis and tumor immune infiltration in hepatocellular
            carcinoma.
REFERENCE   5  (bases 1 to 5905)
  AUTHORS   Spinette,S., Mahoney,J.A. and Rosen,A.
  TITLE     The MPAC domain is a novel mitotically regulated domain, removed by
            apoptotic protease cleavage during cell death
  JOURNAL   Biochem Biophys Res Commun 347 (4), 1103-1112 (2006)
   PUBMED   16870146
  REMARK    GeneRIF: we have identified a novel domain (the MPAC domain:
            Mitotically Phosphorylated, Apoptotically Cleaved) present at the
            N-terminus of Ufd2a, which is regulated both by cleavage during
            cell death, and by phosphorylation during mitosis.
REFERENCE   6  (bases 1 to 5905)
  AUTHORS   Okumura,F., Hatakeyama,S., Matsumoto,M., Kamura,T. and
            Nakayama,K.I.
  TITLE     Functional regulation of FEZ1 by the U-box-type ubiquitin ligase
            E4B contributes to neuritogenesis
  JOURNAL   J Biol Chem 279 (51), 53533-53543 (2004)
   PUBMED   15466860
  REMARK    GeneRIF: E4B serves as a ubiquitin ligase for FEZ1 and thereby
            regulates its function but not its degradation
REFERENCE   7  (bases 1 to 5905)
  AUTHORS   Krona,C., Ejeskar,K., Abel,F., Kogner,P., Bjelke,J., Bjork,E.,
            Sjoberg,R.M. and Martinsson,T.
  TITLE     Screening for gene mutations in a 500 kb neuroblastoma tumor
            suppressor candidate region in chromosome 1p; mutation and
            stage-specific expression in UBE4B/UFD2
  JOURNAL   Oncogene 22 (15), 2343-2351 (2003)
   PUBMED   12700669
  REMARK    GeneRIF: A splice site mutation was found in UBE4b. UBE4B
            expression was diminished in the high-stage/poor-outcome
            tumors.UBE4B stands out as the strongest candidate NBL tumor
            suppressor gene in the region at this stage.
REFERENCE   8  (bases 1 to 5905)
  AUTHORS   Mahoney,J.A., Odin,J.A., White,S.M., Shaffer,D., Koff,A.,
            Casciola-Rosen,L. and Rosen,A.
  TITLE     The human homologue of the yeast polyubiquitination factor Ufd2p is
            cleaved by caspase 6 and granzyme B during apoptosis
  JOURNAL   Biochem J 361 (Pt 3), 587-595 (2002)
   PUBMED   11802788
REFERENCE   9  (bases 1 to 5905)
  AUTHORS   Ohira,M., Kageyama,H., Mihara,M., Furuta,S., Machida,T.,
            Shishikura,T., Takayasu,H., Islam,A., Nakamura,Y., Takahashi,M.,
            Tomioka,N., Sakiyama,S., Kaneko,Y., Toyoda,A., Hattori,M.,
            Sakaki,Y., Ohki,M., Horii,A., Soeda,E., Inazawa,J., Seki,N.,
            Kuma,H., Nozawa,I. and Nakagawara,A.
  TITLE     Identification and characterization of a 500-kb homozygously
            deleted region at 1p36.2-p36.3 in a neuroblastoma cell line
  JOURNAL   Oncogene 19 (37), 4302-4307 (2000)
   PUBMED   10980605
REFERENCE   10 (bases 1 to 5905)
  AUTHORS   Koegl,M., Hoppe,T., Schlenker,S., Ulrich,H.D., Mayer,T.U. and
            Jentsch,S.
  TITLE     A novel ubiquitination factor, E4, is involved in multiubiquitin
            chain assembly
  JOURNAL   Cell 96 (5), 635-644 (1999)
   PUBMED   10089879
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL590639.9, AB028839.1,
            AB014584.3, BC093696.1, AF043117.1, AK292444.1 and BQ001243.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001105562.2.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes an additional conjugation
            factor, E4, which is involved in multiubiquitin chain assembly.
            This gene is also the strongest candidate in the neuroblastoma
            tumor suppressor genes. Alternatively spliced transcript variants
            encoding distinct isoforms have been found for this gene. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF043117.1, SRR1803612.75405.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000343090.11/ ENSP00000343001.6
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AL590639.9         34097-34121
            26-68               AB028839.1         8-50
            69-69               AL590639.9         34165-34165
            70-134              AB028839.1         52-116
            135-635             AB014584.3         1-501
            636-656             BC093696.1         1-21
            657-3664            AF043117.1         29-3036
            3665-4686           BC093696.1         2643-3664
            4687-4702           AK292444.1         3757-3772
            4703-5476           AF043117.1         4075-4848
            5477-5905           BQ001243.1         13-441              c
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.22"
     gene            1..5905
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /note="ubiquitination factor E4B"
                     /db_xref="GeneID:10277"
                     /db_xref="HGNC:HGNC:12500"
                     /db_xref="MIM:613565"
     exon            1..737
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     CDS             714..4622
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitination factor E4B (UFD2 homolog, yeast);
                     homozygously deleted in neuroblastoma-1; ubiquitin-fusion
                     degradation protein 2; homologous to yeast UFD2; ubiquitin
                     conjugation factor E4 B; RING-type E3 ubiquitin
                     transferase E4 B"
                     /codon_start=1
                     /product="ubiquitin conjugation factor E4 B isoform 1"
                     /protein_id="NP_001099032.1"
                     /db_xref="CCDS:CCDS41245.1"
                     /db_xref="GeneID:10277"
                     /db_xref="HGNC:HGNC:12500"
                     /db_xref="MIM:613565"
                     /translation="MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAA
                     SAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSR
                     SQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQ
                     LVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRD
                     ENPFASLTATSQPIAAAARSPDRNLLLNTGSNPGTSPMFCSVASFGASSLSSLYESSP
                     APTPSFWSSVPVMGPSLASPSRAASQLAVPSTPLSPHSAASGTAAGSQPSSPRYRPYT
                     VTHPWASSGVSILSSSPSPPALASSPQAVPASSSRQRPSSTGPPLPPASPSATSRRPS
                     SLRISPSLGASGGASNWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQ
                     PAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRT
                     THQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLV
                     ASLRLWLPKSLSPGCGRELQRLSYLGAFFSFSVFAEDDVKVVEKYFSGPAITLENTRV
                     VSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAAVVNANMKKAQMQTDDRLVS
                     TDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLTE
                     LYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNES
                     QWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLR
                     ILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQALYEPCTQDIVMF
                     LVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMK
                     FYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINML
                     INDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLA
                     LATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGF
                     EPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKF
                     KLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHL
                     LNSPTDPFNRQTLTESMLEPVPELKEQIQAWMREKQNSDH"
     exon            738..924
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            925..1060
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1148
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1293
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1294..1522
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1909
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2051
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2052..2152
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2267
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2268..2351
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2352..2408
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2525
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2526..2624
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2625..2738
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2739..2937
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2938..3076
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3077..3176
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3177..3304
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3305..3403
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3404..3639
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3640..3766
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3767..3911
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3912..4046
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            4047..4238
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            4239..4413
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            4414..4560
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     exon            4561..5905
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5877..5882
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /note="hexamer: AATAAA"
     polyA_site      5905
                     /gene="UBE4B"
                     /gene_synonym="E4; HDNB1; UBOX3; UFD2; UFD2A"
                     /note="major polyA site"
ORIGIN      
        1 ccctttcaaa gatggccgcc ctgttgtttt gatgaataat acttggtggg gcgaggggga
       61 aagagtaggg gtggaggggt aggaggattt actcttccag cgagagctac gcgcatccca
      121 tcctccccct cccccctacc cgggctccgg cgtggaggcg gggcgtggcc ggcctgcttt
      181 gggaggggag gggcttccct tacagtgctg ggctctgcca ggacggctgt ggggtcgcct
      241 tacctcgggg tatccactct gcagtcgacc agttcccgcc aggagcaaag ggtaggaagg
      301 agagcaggat ctgctgtagg aacgcagcta ccgcgccact atcacgaaga aacagcaggc
      361 tcggggcacg agacgaactg gagaccgcgc tgcctagctg ggtaacctgg gaagcagagg
      421 gtaataagtg gcgccttaag acaaccctgt agcagcagca gtggcggcca aaggaggctg
      481 ctcagggaac aagcggctgt agtagtctgt ggggcgactg gagtgaccga agccaaggca
      541 gtttagtgcc tctcgtgttc ttatttttta acctctgact atgcaattct gaaacctccc
      601 ccattcgggg gaccagacag cctgatagac accttccact ctccttcctc ccgccgtggt
      661 ctcgagaaca gaaggatctc tccttaacgc ctttcaccat taagaggaaa gcgatggagg
      721 agctgagcgc tgatgagatt cgacggaggc gccttgcacg acttgctggt ggacagacct
      781 ctcagccaac caccccactc acctctcccc agagggagaa ccctccgggg cctcccatag
      841 cggcatcagc cccaggaccc tctcagagtc ttggtctcaa tgtccacaac atgaccccag
      901 ctacctcccc aataggtgca tcaggagtag cccatcgaag ccagagcagt gaaggagtca
      961 gttctctcag cagctcgccc tctaatagcc ttgaaacgca atctcagtct ctctcacgtt
     1021 cccagagcat ggatatcgat ggtgtctcat gtgagaaaag catgtcccag gtggatgtgg
     1081 attcaggaat tgaaaacatg gaggttgatg aaaatgatcg aagagaaaag cggagcctca
     1141 gtgataagga gccttcctcg ggccctgaag tgtctgaaga gcaggcctta cagctggtct
     1201 gtaagatctt ccgtgtctct tggaaggacc gggacagaga tgtcatcttt ctttcttctc
     1261 tttctgcaca gtttaagcag aacccaaaag aagtattctc cgattttaag gacttgattg
     1321 gccagatttt aatggaagtg ctaatgatgt ccactcagac cagagatgaa aacccatttg
     1381 ccagtctgac agccacatca cagccaattg ctgcagcagc acggtcacca gacagaaatc
     1441 tcttgctaaa cactggctcc aatccaggaa caagccccat gttctgcagc gtggcttcct
     1501 ttggtgccag ctctttgtct agcctctatg aaagtagtcc ggctcccact cccagtttct
     1561 ggagctctgt tcccgtgatg ggcccgtctc ttgcctcacc ttcccgtgca gccagccagt
     1621 tggctgtgcc ttccactccc ctcagtcctc acagtgcagc ctctggaact gctgcgggaa
     1681 gccagccttc atccccgcgg tatcgcccct acactgtcac tcacccatgg gcgtcctcag
     1741 gcgtctccat tctgtcgagc tccccaagtc cccctgccct cgccagtagc ccccaagcag
     1801 tgcccgccag cagttccaga cagaggccca gcagcacggg tccaccccta ccacccgcct
     1861 cacccagtgc cacgagcaga cgcccctcct ccctgaggat ctctcctagt ttgggagcct
     1921 ctggtggagc aagtaattgg gattcctaca gtgaccattt caccattgaa acctgcaaag
     1981 agacagatat gctgaactac ctcatcgagt gtttcgaccg agttggaata gaggaaaaaa
     2041 aagcaccaaa gatgtgcagc cagccagcag tcagccagct tctgagcaac atccgctcac
     2101 agtgcatatc ccatactgct ttagtactac aaggctccct aacacagccc aggtccttgc
     2161 agcagccgtc cttcctagtg ccgtatatgc tgtgtaggaa tctcccatat ggcttcattc
     2221 aggaactggt gagaaccact caccaggatg aagaagtgtt caagcagata tttatcccca
     2281 ttttacaagg cctggctctt gctgccaaag agtgctccct cgacagtgac tactttaaat
     2341 accccctcat ggcactaggt gagctctgtg aaaccaagtt tgggaagaca caccctgtgt
     2401 gcaatttggt tgcttctttg cggttgtggt tgccgaaatc cttaagtcct ggctgtgggc
     2461 gggagctgca gagactctct tacttagggg ctttctttag cttctcagtc tttgcagaag
     2521 atgatgttaa agtggttgaa aaatacttct cagggcctgc cattaccctg gaaaacactc
     2581 gtgtggttag ccaatcattg cagcattact tagagctcgg aaggcaagag ctttttaaga
     2641 ttctgcatag tattttgtta aatggcgaaa cccgtgaggc tgctctcagt tacatggcgg
     2701 ctgtcgtcaa tgccaatatg aagaaagcac agatgcagac agatgataga ttggtgtcta
     2761 cagatggatt tatgctgaat ttcctttggg tactgcagca gctaagtaca aaaatcaagt
     2821 tagaaacagt tgatcccacg tatatttttc acccaagatg tcggattact cttcccaatg
     2881 atgagacgcg tgtgaatgca acgatggaag atgtgaatga ctggctgact gaactctatg
     2941 gcgatcagcc tccattttct gagccgaaat tccctacgga gtgcttcttt ctcaccctgc
     3001 atgctcacca cctctctatt ctgcctagtt gccgtcgcta tatccgcaga ctccgggcta
     3061 tccgggagct caatagaact gtagaagatt tgaaaaataa tgaaagccaa tggaaagatt
     3121 ccccactggc aactagacac cgcgaaatgc tgaagcgctg taaaactcag cttaagaaac
     3181 tggtacggtg caaggcctgt gctgatgctg gcctacttga cgagagcttc ctgagaagat
     3241 gtctgaattt ttatggcctt ctcattcagc tgctgctccg catcctggac cccgcatatc
     3301 ccgatataac actgccttta aattcagatg tccccaaggt atttgcagcg ttgcctgagt
     3361 tttatgtaga agatgttgca gaatttttat tttttattgt acaatactct ccccaggcgc
     3421 tttatgagcc ctgtactcag gatattgtga tgttccttgt tgtgatgttg tgcaaccaga
     3481 actacatccg aaacccatat ttggtggcca aactggtaga agtcatgttt atgaccaacc
     3541 ctgctgttca gccacgaacc cagaagtttt ttgaaatgat tgagaaccat cctctctcca
     3601 ccaagttgtt ggtaccttcc ctgatgaagt tttatacaga tgttgagcat accggagcca
     3661 ccagtgagtt ttatgacaag ttcacaattc gctatcatat tagcaccatt tttaaaagcc
     3721 tttggcaaaa catagctcac catggcacct ttatggagga gttcaactcc gggaagcagt
     3781 ttgttcgcta tataaacatg ttgataaacg acacgacgtt tttgctcgat gaaagtctgg
     3841 agtctctgaa gcgaatccat gaagtgcagg aagagatgaa gaacaaagaa cagtgggacc
     3901 agttgccccg ggatcagcag caggctcgtc agtctcagct tgctcaggat gagcgtgtgt
     3961 cccgctctta cctcgccctg gccaccgaaa ccgtggacat gttccacatc ctcacgaagc
     4021 aggtccagaa gcccttcctc agaccggagc ttggaccccg attggctgca atgctgaact
     4081 ttaatcttca gcaactttgt ggccccaagt gccgtgacct gaaagttgaa aaccctgaga
     4141 aatacggctt tgaaccaaag aagctgttgg accaactgac ggatatttac ttacagctgg
     4201 actgtgctcg gttcgcgaaa gccattgctg acgaccagag atcctacagt aaggaattgt
     4261 ttgaagaagt tatttcaaag atgcggaagg cagggatcaa atccacaata gcaatagaaa
     4321 aatttaagct gctcgccgag aaagtggagg agatagtggc caagaacgca cgcgcagaaa
     4381 tcgactacag cgacgctcct gatgagttca gagaccctct gatggacacc ctcatgacag
     4441 accccgtgcg gctgccctct ggcaccatca tggaccgctc catcatcctg cggcacctgc
     4501 tcaactcccc cacggacccc ttcaaccggc agacgctgac agagagcatg ctggaaccag
     4561 tgccagaact gaaagagcag attcaggcgt ggatgagaga gaaacagaac agcgatcact
     4621 aaaccgttcc gccgcccacc ctctgctaga cacagccaag gccaacgagg caagcagaag
     4681 cagcggccgc agcgaagctg ccgttcatgt gttggaggcc aaatgtggca aaccaacccc
     4741 aggcccaccc agagcgagca aacgctgaga cctgaaagga catggatgag aagaggagcc
     4801 cgcttcctgt acatatattt aagtgacaaa cacggtcaaa agcttaaggg acaggtttta
     4861 tggttgcttg tgtaataaag catgtccttc gtatgtcaca gtttggggca acggaagtct
     4921 tttagtgatg gctaatgggt ctgggcagca tcccttcatg aatttttttt taatccaata
     4981 tccgttgatt tgattgtgat tagagaacct tggacatttt gctgctaaag aatctttttt
     5041 cccctctccc ccttcctgac cctacttgca ctgctgtgga tttttttaag agaagcaaaa
     5101 acaaaagtaa actcctttcc ctggccctcc aaacatatat tctgtgagat aactgtgcct
     5161 gctaccaagt gttaatcctg ggatcgtatt tttatatcat attcacatat ttgttttttt
     5221 aattggtgtt agatgacatg attaataaaa aaggcaagat attttcagaa tttgaatttc
     5281 agtttttttt ttcttttgaa atgtcccttt aagatttttt tattctaaac aaaataaaga
     5341 aaatcctgct gctctggtcc ttgtgataag cctctcttcg gcatctgagg agcagctgca
     5401 gcaaaatcta ggggtgtaag tgtatcagga cttatgtgac ttatattttg gggagatgag
     5461 ggttgggttt tttttttaat gctacgtgac agtttgaaac cttcacagtt atcctctggg
     5521 ggtaaatcaa ttcttcaacc cttgggtgtg tgagtttgag gcagggtcat ttgtgtgatg
     5581 tgtttggcct taccaaagca aaagagggtg caagaatgtg ggagtatgtc tgccggtttc
     5641 aacacacaca gacaaacaca gccacacgcg cacacaagta taagactttt tgtattactg
     5701 ctccctactt aacatacttg aattctcaaa tttcctttgg ggtaaaaaaa aaaaaaggat
     5761 ttgaaaccat aaagtgttct gaagaaatta agtctataaa aagcatactt tcttttttct
     5821 tttccttttt tccctccaca gacaatgtcc tctgttcaat tcctaacgca aactacaata
     5881 aatggtgaca cacgttcaga agaaa
//
