LOCUS       NM_001204077            6088 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant
            2, mRNA.
ACCESSION   NM_001204077
VERSION     NM_001204077.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Hu,X., Zou,R., Zhang,Z., Ji,J., Li,J., Huo,X.Y., Liu,D., Ge,M.X.,
            Cui,M.K., Wu,M.Z., Li,Z.P., Wang,Q., Zhang,X. and Zhang,Z.R.
  TITLE     UBE4A catalyzes NRF1 ubiquitination and facilitates DDI2-mediated
            NRF1 cleavage
  JOURNAL   Biochim Biophys Acta Gene Regul Mech 1866 (2), 194937 (2023)
   PUBMED   37084817
  REMARK    GeneRIF: UBE4A catalyzes NRF1 ubiquitination and facilitates
            DDI2-mediated NRF1 cleavage.
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Xie,C., Long,F., Li,L., Li,X., Ma,M., Lu,Z., Wu,R., Zhang,Y.,
            Huang,L., Chou,J., Gong,N., Hu,G. and Lin,C.
  TITLE     PTBP3 modulates P53 expression and promotes colorectal cancer cell
            proliferation by maintaining UBE4A mRNA stability
  JOURNAL   Cell Death Dis 13 (2), 128 (2022)
   PUBMED   35136024
  REMARK    GeneRIF: PTBP3 modulates P53 expression and promotes colorectal
            cancer cell proliferation by maintaining UBE4A mRNA stability.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6088)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 6088)
  AUTHORS   Melo,U.S., Bonner,D., Kent Lloyd,K.C., Moshiri,A., Willis,B.,
            Lanoue,L., Bower,L., Leonard,B.C., Martins,D.J., Gomes,F., de Souza
            Leite,F., Oliveira,D., Kitajima,J.P., Monteiro,F.P., Zatz,M.,
            Menck,C.F.M., Wheeler,M.T., Bernstein,J.A., Dumas,K., Spiteri,E.,
            Di Donato,N., Jahn,A., Hashem,M., Alsaif,H.S., Chedrawi,A.,
            Alkuraya,F.S., Kok,F. and Byers,H.M.
  TITLE     Biallelic UBE4A loss-of-function variants cause intellectual
            disability and global developmental delay
  JOURNAL   Genet Med 23 (4), 661-668 (2021)
   PUBMED   33420346
  REMARK    GeneRIF: Biallelic UBE4A loss-of-function variants cause
            intellectual disability and global developmental delay.
REFERENCE   5  (bases 1 to 6088)
  AUTHORS   Irvin,M.R., Shrestha,S., Chen,Y.D., Wiener,H.W., Haritunians,T.,
            Vaughan,L.K., Tiwari,H.K., Taylor,K.D., Scherzer,R., Saag,M.S.,
            Grunfeld,C., Rotter,J.I. and Arnett,D.K.
  TITLE     Genes linked to energy metabolism and immunoregulatory mechanisms
            are associated with subcutaneous adipose tissue distribution in
            HIV-infected men
  JOURNAL   Pharmacogenet Genomics 21 (12), 798-807 (2011)
   PUBMED   21897333
REFERENCE   6  (bases 1 to 6088)
  AUTHORS   Sakiyama,T., Fujita,H. and Tsubouchi,H.
  TITLE     Autoantibodies against ubiquitination factor E4A (UBE4A) are
            associated with severity of Crohn's disease
  JOURNAL   Inflamm Bowel Dis 14 (3), 310-317 (2008)
   PUBMED   18069675
  REMARK    GeneRIF: Identified an intestinal cDNA clone encoding
            ubiquitination factor E4A (UBE4A), a U-box-type ubiquitin-protein
            ligase. The prevalence of anti-UBE4A IgG in patients with CD was
            significantly higher than that in patients with UC or healthy
            controls
REFERENCE   7  (bases 1 to 6088)
  AUTHORS   Yang,Y., Hou,J.Q., Qu,L.Y., Wang,G.Q., Ju,H.W., Zhao,Z.W., Yu,Z.H.
            and Yang,H.J.
  TITLE     [Differential expression of USP2, USP14 and UBE4A between ovarian
            serous cystadenocarcinoma and adjacent normal tissues]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 23 (6), 504-506 (2007)
   PUBMED   17553343
  REMARK    GeneRIF: UBE4A was over-expressed in ovarian serous
            cystadenocarcinoma tissues, suggesting that the activity of
            ubiquitin-proteasome system is enhanced in ovarian cancer.
REFERENCE   8  (bases 1 to 6088)
  AUTHORS   Spinette,S., Lengauer,C., Mahoney,J.A., Jallepalli,P.V., Wang,Z.,
            Casciola-Rosen,L. and Rosen,A.
  TITLE     Ufd2, a novel autoantigen in scleroderma, regulates sister
            chromatid separation
  JOURNAL   Cell Cycle 3 (12), 1638-1644 (2004)
   PUBMED   15611659
  REMARK    GeneRIF: In these studies, we define Ufd2 (a novel E4
            polyubiquitylating enzyme) as a new autoantigen in scleroderma, and
            show that it regulates chromosome condensation and separation
            during mitosis in human cells.
REFERENCE   9  (bases 1 to 6088)
  AUTHORS   Contino,G., Amati,F., Pucci,S., Pontieri,E., Pichiorri,F.,
            Novelli,A., Botta,A., Mango,R., Nardone,A.M., Sangiuolo,F.C.,
            Citro,G., Spagnoli,L.G. and Novelli,G.
  TITLE     Expression analysis of the gene encoding for the U-box-type
            ubiquitin ligase UBE4A in human tissues
  JOURNAL   Gene 328, 69-74 (2004)
   PUBMED   15019985
  REMARK    GeneRIF: UBE4A is expressed in different tissues in a pattern that
            seems to be dependent from cell type and cell cycle and that UBE4A
            might have a specific role in different biochemical processes other
            than ubiquitination, including growth or differentiation.
REFERENCE   10 (bases 1 to 6088)
  AUTHORS   Koegl,M., Hoppe,T., Schlenker,S., Ulrich,H.D., Mayer,T.U. and
            Jentsch,S.
  TITLE     A novel ubiquitination factor, E4, is involved in multiubiquitin
            chain assembly
  JOURNAL   Cell 96 (5), 635-644 (1999)
   PUBMED   10089879
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB068702.1, BC112367.1,
            AP001267.4, BC039240.1 and AW021567.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001204077.1.
            
            Summary: This gene encodes a member of the U-box ubiquitin ligase
            family. The encoded protein is involved in multiubiquitin chain
            assembly and plays a critical role in chromosome condensation and
            separation through the polyubiquitination of securin.
            Autoantibodies against the encoded protein may be markers for
            scleroderma and Crohn's disease. A pseudogene of this gene is
            located on the long arm of chromosome 3. Alternatively spliced
            transcript variants encoding multiple isoforms have been observed
            for this gene. [provided by RefSeq, Feb 2011].
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the coding region, but maintains the reading frame, compared to
            variant 1. The encoded isoform (2) is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.98114.1,
                                           SRR1803614.251633.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000252108.8/ ENSP00000252108.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 DB068702.1         20-23
            5-3441              BC112367.1         1-3437
            3442-3442           AP001267.4         22003-22003
            3443-5978           BC039240.1         843-3378
            5979-6088           AW021567.1         8-117
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.3"
     gene            1..6088
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /note="ubiquitination factor E4A"
                     /db_xref="GeneID:9354"
                     /db_xref="HGNC:HGNC:12499"
                     /db_xref="MIM:603753"
     exon            1..75
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            76..237
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..3317
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /EC_number="2.3.2.27"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ubiquitination factor E4A (UFD2 homolog, yeast); ubiquitin
                     conjugation factor E4 A; RING-type E3 ubiquitin
                     transferase E4 A"
                     /codon_start=1
                     /product="ubiquitin conjugation factor E4 A isoform 2"
                     /protein_id="NP_001191006.1"
                     /db_xref="CCDS:CCDS55790.1"
                     /db_xref="GeneID:9354"
                     /db_xref="HGNC:HGNC:12499"
                     /db_xref="MIM:603753"
                     /translation="MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELP
                     ASPDDSDNSVSESLDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPS
                     LKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQALFARLLLQDPGNHLINMTSSTTL
                     NLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPE
                     IYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALILDEEVRTFPEVMIPVFDIL
                     LGRIKDLELCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVIL
                     SISCLLKTPGVVENHGYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLS
                     PETKHCILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFC
                     KPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKFPQNYNLVT
                     ENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMT
                     IYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYV
                     PEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAE
                     VLEAVMPHLDQTPNPLVSSVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQ
                     KFNYRRPMYPILRYMWGTDTYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFL
                     LDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMSNETIGT
                     LAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSD
                     ICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMAFSNLAERIK
                     SLADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDP
                     FNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE"
     exon            238..411
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            412..524
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            525..677
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            678..837
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            838..1040
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1232
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1566
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..1687
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1992
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2125
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2313
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2314..2414
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2415..2528
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2703
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2704..2884
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            2885..3032
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3033..3190
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     exon            3191..6088
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6065..6070
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /note="hexamer: AATAAA"
     regulatory      6069..6074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /note="hexamer: AATGAA"
     polyA_site      6085
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /note="major polyA site"
     polyA_site      6088
                     /gene="UBE4A"
                     /gene_synonym="E4; NEDHMS; UBOX2; UFD2"
                     /note="major polyA site"
ORIGIN      
        1 gtgctgagtc ggaagtggga acccttcggc cgctgagatt ctgtcgtgtc gtcgctgctg
       61 gcacttcagg ctctgcctct cccactaggt ctggatggag gataccttaa agtgaaatga
      121 cagaccagga gaataacaac aacatctcaa gtaacccctt tgctgctctt tttggctccc
      181 tggctgatgc caaacagttt gcggcaatcc aaaaagagca gctgaagcaa caatctgatg
      241 aactcccagc tagcccagat gactcggata atagcgtgtc agagagcctg gatgaattcg
      301 attactctgt ggctgagatt agccgctcat tccgatcaca gcaggaaata tgtgagcaac
      361 tcaacatcaa tcacatgatc caaaggatct tccttattac tctggacaac agtgatccca
      421 gcttgaaaag cgggaatggc atccctagcc gttgtgtgta tttggaagaa atggcagtag
      481 agctagaaga tcaagactgg cttgatatga gcaatgttga gcaggccctc ttcgctcgct
      541 tattacttca agatccaggc aaccacttaa ttaacatgac ttcttctaca acgctaaatc
      601 tctctgctga tcgagatgca ggagagaggc acattttttg ttacctttac tcctgcttcc
      661 agagagccaa ggaagagatt accaaagttc cagagaacct gctacccttt gcagtgcagt
      721 gcagaaacct cactgtgtcc aatacccgaa cagttcttct caccccagag atctatgttg
      781 accaaaacat ccatgagcaa ctggtagatt tgatgttaga agccatccag ggagcccatt
      841 ttgaagatgt aactgagttt ctggaagagg tcattgaagc cttgatattg gatgaggaag
      901 ttagaacatt tccagaagtc atgattccag tgtttgatat tttattgggc cgaataaaag
      961 atctagagct ctgtcagatc cttttgtatg catatctgga tattcttctc tatttcacta
     1021 ggcaaaaaga tatggcaaag gtttttgtag aatacattca gcccaaggac cctaccaatg
     1081 ggcaaatgta ccagaagacc ttgctgggag taattctgag tatctcctgc ttattaaaga
     1141 ctccgggtgt tgtagaaaat catggctact ttttgaatcc atctcgttcc agcccccagg
     1201 agatcaaagt acaggaggcc aacatccatc agttcatggc tcagttccac gaaaagatct
     1261 accagatgct gaagaactta ctccagctct ctccagaaac caaacactgt atcttgtcct
     1321 ggcttggaaa ctgtttgcat gcaaatgcag gccgcaccaa gatttgggcc aatcagatgc
     1381 cagaaatctt tttccaaatg tatgcctcag atgctttctt tctgaatctg ggtgctgctc
     1441 tcctgaagct atgccagcca ttttgcaaac ccagatcctc tcggctcctc acctttaatc
     1501 ccacatactg tgccctcaag gagttgaatg atgaagaacg aaaaattaaa aatgtacaca
     1561 tgagaggttt ggacaaagaa acctgtttga tcccagctgt gcaggagccg aagtttccac
     1621 agaactacaa ccttgtaaca gagaaccttg ctctgacaga gtacaccttg tacttgggat
     1681 ttcacaggtt gcatgatcag atggtaaaaa tcaaccaaaa tctgcatcgg ctgcaggttg
     1741 cctggcggga tgctcagcaa agttctagcc ctgctgctga caatcttcgt gagcagtttg
     1801 aacgactgat gaccatctat ctttctacca agactgccat gacagagcca caaatgctac
     1861 aaaactgcct aaacttgcag gtgtccatgg ctgttctact ggttcaactg gccataggca
     1921 atgagggctc acagccaata gagctaacct ttcctttgcc agatggctac agctctttgg
     1981 cttatgtgcc agaatttttt gcagataacc tgggtgattt tctcattttt ctccgccgct
     2041 ttgccgatga cattttggag acatcagcag attccctgga gcatgtcctt cactttatca
     2101 ccattttcac tggaagcata gaaagaatga agaatcccca cctgagggcc aaactagcag
     2161 aggtgttgga agcagtgatg ccccacctgg atcagacccc aaatcccttg gtatccagtg
     2221 tgttccaccg gaaacgtgtg ttctgcaact ttcagtatgc accccaactt gcagaggctc
     2281 taatcaaggt ttttgtggac atcgaattta caggagaccc ccatcaattt gaacagaagt
     2341 ttaattaccg ccgtcccatg tatcctatcc taagatacat gtgggggaca gatacctatc
     2401 gggagagcat taaggatttg gctgactatg cctctaagaa tttagaagcc atgaatcccc
     2461 cacttttcct ccgctttctt aacctgctaa tgaatgatgc catcttcctt ttggatgaag
     2521 ccatacagta tttgagcaag ataaagattc agcaaattga gaaggatcga ggtgaatggg
     2581 atagtctgac tccagaagcc cgccgagaaa aggaggctgg cctacagatg tttggacagc
     2641 tggcacgttt ccataacatc atgtccaatg aaacaatcgg tacccttgcc tttctcacat
     2701 cagagatcaa gtcactcttt gtgcatccct tcctggctga gcgcatcatc tccatgttga
     2761 actacttcct gcaacacctg gttggcccca agatgggtgc cttaaaagtc aaggacttca
     2821 gcgaatttga cttcaaaccc cagcagcttg tatcagatat ctgcactatc tacttaaatc
     2881 ttggggatga ggagaatttc tgtgccactg tgcccaagga tggacgttcc tattccccaa
     2941 ctctctttgc acagacagtt cgagtcttga agaaaataaa taagcctggg aatatgatta
     3001 tggctttcag caacttggca gagagaatca agtctcttgc agacctccaa caacaggaag
     3061 aggaaaccta tgcagatgcc tgtgatgagt tcctggatcc cattatgagc acactgatgt
     3121 gtgaccctgt ggtgctgcca tcttccagag tcactgtgga tagatccacc attgcaagac
     3181 atttgctcag tgaccaaaca gatcccttta accgtagtcc cctcaccatg gaccagatcc
     3241 ggccaaacac agaactaaaa gaaaaaatcc aacggtggct tgcagagagg aaacaacaaa
     3301 aggagcaact tgaatagata ctgtgaacta accaaaccaa aaccaacccc agagtgcaga
     3361 taaacaattg tttgtggttt ctctctttct ggttctgttc cttttctttc ttcttttctt
     3421 tttctttttt tttttttttt ttactaaatt agagaactgc tcttgctgaa attatgatag
     3481 ttaagattcc taagaacttg acaatgctcc cctggcttgc aggaaattat acttattata
     3541 gcatcaccaa gtttagaaat catcactaat tttcaccctc tgttgtctct tcatattctt
     3601 cttccttttc ctaaatgaaa ttatattatt tcaactacta aaacttcccg ccaccctgct
     3661 atttctcttc caattactgt tcaaacattt ttggtgagtg ctgcttttat aaatatgtga
     3721 tgtcacatat ttcagtgaca gctgatgtta tcagaaaaat cctgttatcc tgtgtattta
     3781 ctagtcctca tgatctagac ttaacccctt ttctgtgtta cagagtaact tcacataaaa
     3841 gcagatacct ttaagtttgt gtagacttct gaataagatg atagacgaga atttttttta
     3901 aaaaaggaat ttaaaggaat cttgtgcaac ttctgttgat agctcttaat tttgttatac
     3961 agtcttttta atgaggattg gtagggcaat cctaccaatt gatgggggtg aggagactgt
     4021 tgggccctta ttctcttatg aaaatctgtt tctacaagga ctaggcctaa gtagatttag
     4081 gcaacgggta tcatatccat gaaaaatgtc attttaagac actaatatca acagtatatc
     4141 tcagtgtgtg agatatataa atacacatac accccatgtg cttttttgtt ggtttagtgt
     4201 aaactgagta ccactttata ttttctcttc catagtgaga tgtatgcagt atgtggccat
     4261 gtttactaac atactcattc ttcacaaaat tctgagatta taaaatgtat atagttaata
     4321 tttgtttgag cttgtgacct gacttctaag agctacttct aacacagcct taggtcttca
     4381 attaacaaag taggaagttg agcacaacct tgtccacagc cattttaaat atccacttag
     4441 ccctgtgtag ttggtaggtg gacaccactt tcataagtga acttgagctc actactcaat
     4501 tgtgcagctt aatatgctga ctagggacat tcccctatgg attatcttta tatcactgtc
     4561 tttctaagcc cagagatgtc ataagtgaca agaaaagtga taggatcaag aaatgggtgt
     4621 cactgcttca tttggaacat gttattaact atggtctctt tcaaataaat agtagtttta
     4681 tatttcaaca caagttgcta taagcagtcc ttgatgggtt tttgattgca tcagctggaa
     4741 agcctcaaat ctaagagggc ttcccatcct agatataaaa taggtgttgc ctattgctgt
     4801 gcttataaaa tgaaaaagga aattgaggac acttttgcaa atgccagaat gtaagattca
     4861 ttcagtgtgc tccctgggcc tttatggcat gggttgacag gatttgttta ttttctaaaa
     4921 ttagcttcat tcaatattta tcatcctcct ttccctctct gagaatgaac tatgtataaa
     4981 ataagcttct gcctatttgc atttatcttc caaacccaat ctagtaggat gttctcattt
     5041 taaaaacgag gggaaaagac cagagttttt caggagaaaa ctggaggaaa atgggcacaa
     5101 aaactcagaa ggcagctatt cccagcagct tcctagttaa caacccccat gctgcctcca
     5161 gtctttgtct gtattcttct gtatttaacc ttcagattgt aagccttttc tggcaagctt
     5221 ttcttctttt tttaaactct tttcctgaaa ctttttatga atggctatgg caccattaat
     5281 gctgctgaat atctttaaac tctgcacaag caagtgtgta gcttaaggcc actactggta
     5341 aggaaaccaa gtgtcctctg tgcctttttt ctttctgtga agtaatttaa gaatatccaa
     5401 aaaaattaga ctttaaaaag ttatcttggt acaacaccgt gtgtatatac acttggaagc
     5461 ttaaaaaggt gttttgtctg gaacttagaa gcagctctaa atctagtaga gcagactttc
     5521 taacatacct agttttgtgt attggctttg ctggagtatg atagcaaaat gaagactctt
     5581 ttactcagct ctggtattgc tcataactta ccaagaggct aatactaaac ttggaaaatt
     5641 gtttaagtat gttttatcaa gcagtctggg ttttgttttt taatatactt tttaatggat
     5701 atgtgaaaac tgaaggaaat gttaaaggtt ttttaatggt gcaagtgaag gtgccagttg
     5761 ctatttgata tcacactcta caaaagcttc attactttat ttgatggtgg ttgctaagca
     5821 gccattgcac agagcataag tctactgggt gcctttacat gccagaggct gatgctgcac
     5881 tgttgatgtc atgtgaggaa ataatgcaca tgctctaact gctcaacagg aaatgaacct
     5941 agaaacagaa aatgaaaagg ttgattgaaa taaaacttga tcaacgcgac tgtattttga
     6001 aacattccag gaaggttact tcttgtcaaa cttgcctggc agtgtttgtt caaaacttgt
     6061 atttaataaa tgaacatctg acttacaa
//
