LOCUS       NM_130839               8728 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens ubiquitin protein ligase E3A (UBE3A), transcript
            variant 3, mRNA.
ACCESSION   NM_130839
VERSION     NM_130839.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Krzeski,J.C., Judson,M.C. and Philpot,B.D.
  TITLE     Neuronal UBE3A substrates hold therapeutic potential for Angelman
            syndrome
  JOURNAL   Curr Opin Neurobiol 88, 102899 (2024)
   PUBMED   39126903
  REMARK    GeneRIF: Neuronal UBE3A substrates hold therapeutic potential for
            Angelman syndrome.
            Review article
REFERENCE   2  (bases 1 to 8728)
  AUTHORS   Bruns,R., Liaqat,K., Nasir,A., Treat,K., Murthy,V.S., Mantcheva,L.,
            Torres,W., Conboy,E. and Vetrini,F.
  TITLE     Undiagnosed rare disease clinic identifies a novel UBE3A variant in
            two sisters with Angelman syndrome: The end of a diagnostic odyssey
  JOURNAL   Congenit Anom (Kyoto) 64 (3), 155-160 (2024)
   PUBMED   38520260
  REMARK    GeneRIF: Undiagnosed rare disease clinic identifies a novel UBE3A
            variant in two sisters with Angelman syndrome: The end of a
            diagnostic odyssey.
REFERENCE   3  (bases 1 to 8728)
  AUTHORS   Li,M., Chen,J., Zhang,H., Zhang,Y., Wang,J., Shen,Z., Chen,Y.,
            Hou,W. and Chi,C.
  TITLE     LOC644656 promotes cisplatin resistance in cervical cancer by
            recruiting ZNF143 and activating the transcription of E6-AP
  JOURNAL   Cell Signal 117, 111115 (2024)
   PUBMED   38395183
  REMARK    GeneRIF: LOC644656 promotes cisplatin resistance in cervical cancer
            by recruiting ZNF143 and activating the transcription of E6-AP.
REFERENCE   4  (bases 1 to 8728)
  AUTHORS   Wei,Z., Zhang,C., Song,Y., Han,D., Liu,J., Song,X., Chao,F.,
            Wang,S., Xu,G. and Chen,G.
  TITLE     CircUBE3A(2,3,4,5) promotes adenylate-uridylate-rich binding factor
            1 nuclear translocation to suppress prostate cancer metastasis
  JOURNAL   Cancer Lett 588, 216743 (2024)
   PUBMED   38423246
  REMARK    GeneRIF: CircUBE3A(2,3,4,5) promotes adenylate-uridylate-rich
            binding factor 1 nuclear translocation to suppress prostate cancer
            metastasis.
REFERENCE   5  (bases 1 to 8728)
  AUTHORS   Rougeulle,C., Glatt,H. and Lalande,M.
  TITLE     The Angelman syndrome candidate gene, UBE3A/E6-AP, is imprinted in
            brain
  JOURNAL   Nat Genet 17 (1), 14-15 (1997)
   PUBMED   9288088
REFERENCE   6  (bases 1 to 8728)
  AUTHORS   Vu,T.H. and Hoffman,A.R.
  TITLE     Imprinting of the Angelman syndrome gene, UBE3A, is restricted to
            brain
  JOURNAL   Nat Genet 17 (1), 12-13 (1997)
   PUBMED   9288087
  REMARK    GeneRIF: UBE3A gene exhibits tissue-specific imprinting, with
            monoallelic expression from the maternal allele only in brain, but
            biallelic expression in other tissues.
REFERENCE   7  (bases 1 to 8728)
  AUTHORS   Chen,J.J., Reid,C.E., Band,V. and Androphy,E.J.
  TITLE     Interaction of papillomavirus E6 oncoproteins with a putative
            calcium-binding protein
  JOURNAL   Science 269 (5223), 529-531 (1995)
   PUBMED   7624774
REFERENCE   8  (bases 1 to 8728)
  AUTHORS   Huibregtse,J.M., Scheffner,M., Beaudenon,S. and Howley,P.M.
  TITLE     A family of proteins structurally and functionally related to the
            E6-AP ubiquitin-protein ligase
  JOURNAL   Proc Natl Acad Sci U S A 92 (7), 2563-2567 (1995)
   PUBMED   7708685
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 1995 May 23;92(11):5249. doi:
            10.1073/pnas.92.11.5249-b.. PMID: 7761480]
REFERENCE   9  (bases 1 to 8728)
  AUTHORS   Scheffner,M., Huibregtse,J.M., Vierstra,R.D. and Howley,P.M.
  TITLE     The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein
            ligase in the ubiquitination of p53
  JOURNAL   Cell 75 (3), 495-505 (1993)
   PUBMED   8221889
REFERENCE   10 (bases 1 to 8728)
  AUTHORS   Dagli,A.I., Mathews,J. and Williams,C.A.
  TITLE     Angelman Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301323
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC124997.4, BC002582.2,
            AK292514.1 and AC100774.2.
            
            On Nov 5, 2019 this sequence version replaced NM_130839.4.
            
            Summary: This gene encodes an E3 ubiquitin-protein ligase, part of
            the ubiquitin protein degradation system. This imprinted gene is
            maternally expressed in brain and biallelically expressed in other
            tissues. Maternally inherited deletion of this gene causes Angelman
            Syndrome, characterized by severe motor and intellectual
            retardation, ataxia, hypotonia, epilepsy, absence of speech, and
            characteristic facies. The protein also interacts with the E6
            protein of human papillomavirus types 16 and 18, resulting in
            ubiquitination and proteolysis of tumor protein p53. Alternative
            splicing of this gene results in three transcript variants encoding
            three isoforms with different N-termini. Additional transcript
            variants have been described, but their full length nature has not
            been determined. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (3) differs in the 5' UTR, and
            uses an alternate start codon compared to variant 2. The encoded
            isoform (3) has a distinct N-terminus and is 3 and is shorter than
            isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.1639150.1, BC002582.2
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 9288087, 9288088
            MANE Ensembl match     :: ENST00000648336.2/ ENSP00000497572.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                AC124997.4         82666-82708         c
            44-2980             BC002582.2         1-2937
            2981-2988           AK292514.1         2595-2602
            2989-3365           BC002582.2         2946-3322
            3366-8728           AC100774.2         130125-135487       c
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q11.2"
     gene            1..8728
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /note="ubiquitin protein ligase E3A"
                     /db_xref="GeneID:7337"
                     /db_xref="HGNC:HGNC:12496"
                     /db_xref="MIM:601623"
     exon            1..536
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            537..600
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            601..720
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             701..3319
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /EC_number="2.3.2.26"
                     /note="isoform 3 is encoded by transcript variant 3;
                     oncogenic protein-associated protein E6-AP; CTCL tumor
                     antigen se37-2; human papilloma virus E6-associated
                     protein; E6AP ubiquitin-protein ligase; renal carcinoma
                     antigen NY-REN-54; human papillomavirus E6-associated
                     protein; HECT-type ubiquitin transferase E3A"
                     /codon_start=1
                     /product="ubiquitin-protein ligase E3A isoform 3"
                     /protein_id="NP_570854.1"
                     /db_xref="CCDS:CCDS45191.1"
                     /db_xref="GeneID:7337"
                     /db_xref="HGNC:HGNC:12496"
                     /db_xref="MIM:601623"
                     /translation="MATACKRSGEPQSDDIEASRMKRAAAKHLIERYYHQLTEGCGNE
                     ACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGAPN
                     NSCSEIKMNKKGARIDFKDVTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEAL
                     VQSFRKVKQHTKEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGD
                     NNLQKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDLTYHNVYSR
                     DPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLIRLWSKYNADQIR
                     RMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGEVDTNHNEE
                     DDEEPIPESSELTLQELLGEERRNKKGPRVDPLETELGVKTLDCRKPLIPFEEFINEP
                     LNEVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLY
                     SLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGG
                     VSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIYNNC
                     ILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVEDDMMITFQISQT
                     DLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSVEKQFKAFRRGFHMVTNE
                     SPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRDSVLIREFWEIVHSFTDEQ
                     KRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLK
                     ERLLKAITYAKGFGML"
     exon            721..762
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            763..1061
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..2308
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2309..2453
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2454..2659
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..2824
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2825..2980
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            2981..3054
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            3055..3198
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..8728
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5192..5197
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /note="hexamer: AATAAA"
     polyA_site      5209
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /note="major polyA site"
     regulatory      8703..8708
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
                     /note="hexamer: CATAAA"
     polyA_site      8728
                     /gene="UBE3A"
                     /gene_synonym="ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1"
ORIGIN      
        1 agtcctcccg tcttgcgccg cggccgcgag atccgtgtgt ctcccaagat ggtggcgctg
       61 ggctcggggt gactacagga gacgacgggg ccttttccct tcgccaggac ccgacacacc
      121 aggcttcgct cgctcgcgca cccctccgcc gcgtagccat ccgccagcgc gggcgcccgc
      181 catccgccgc ctacttacgc ttcacctctg ccgacccggc gcgctcggct gcgggcggcg
      241 gcgcctcctt cggctcctcc tcggaatagc tcgcggcctg tagcccctgg caggagggcc
      301 cctcagcccc ccggtgtgga caggcagcgg cggctggcga cgaacgccgg gatttcggcg
      361 gccccggcgc tccctttccc ggcctcgttt tccggataag gaagcgcggg tcccgcatga
      421 gccccggcgg tggcggcagc gaaagagaac gaggcggtgg cgggcggagg cggcgggcga
      481 gggcgactac gaccagtgag gcggccgccg cagcccaggc gcgggggcga cgacaggtta
      541 aaaatctgta agagcctgat tttagaattc accagctcct cagaagtttg gcgaaatatg
      601 agttattaag cctacgctca gatcaaggta gcagctagac tggtgtgaca acctgttttt
      661 aatcagtgac tcaaagctgt gatcaccctg atgtcaccga atggccacag cttgtaaaag
      721 atcaggagaa cctcagtctg acgacattga agctagccga atgaagcgag cagctgcaaa
      781 gcatctaata gaacgctact accaccagtt aactgagggc tgtggaaatg aagcctgcac
      841 gaatgagttt tgtgcttcct gtccaacttt tcttcgtatg gataataatg cagcagctat
      901 taaagccctc gagctttata agattaatgc aaaactctgt gatcctcatc cctccaagaa
      961 aggagcaagc tcagcttacc ttgagaactc gaaaggtgcc cccaacaact cctgctctga
     1021 gataaaaatg aacaagaaag gcgctagaat tgattttaaa gatgtgactt acttaacaga
     1081 agagaaggta tatgaaattc ttgaattatg tagagaaaga gaggattatt cccctttaat
     1141 ccgtgttatt ggaagagttt tttctagtgc tgaggcattg gtacagagct tccggaaagt
     1201 taaacaacac accaaggaag aactgaaatc tcttcaagca aaagatgaag acaaagatga
     1261 agatgaaaag gaaaaagctg catgttctgc tgctgctatg gaagaagact cagaagcatc
     1321 ttcctcaagg ataggtgata gctcacaggg agacaacaat ttgcaaaaat taggccctga
     1381 tgatgtgtct gtggatattg atgccattag aagggtctac accagattgc tctctaatga
     1441 aaaaattgaa actgcctttc tcaatgcact tgtatatttg tcacctaacg tggaatgtga
     1501 cttgacgtat cacaatgtat actctcgaga tcctaattat ctgaatttgt tcattatcgt
     1561 aatggagaat agaaatctcc acagtcctga atatctggaa atggctttgc cattattttg
     1621 caaagcgatg agcaagctac cccttgcagc ccaaggaaaa ctgatcagac tgtggtctaa
     1681 atacaatgca gaccagattc ggagaatgat ggagacattt cagcaactta ttacttataa
     1741 agtcataagc aatgaattta acagtcgaaa tctagtgaat gatgatgatg ccattgttgc
     1801 tgcttcgaag tgcttgaaaa tggtttacta tgcaaatgta gtgggagggg aagtggacac
     1861 aaatcacaat gaagaagatg atgaagagcc catccctgag tccagcgagc tgacacttca
     1921 ggaacttttg ggagaagaaa gaagaaacaa gaaaggtcct cgagtggacc ccctggaaac
     1981 tgaacttggt gttaaaaccc tggattgtcg aaaaccactt atcccttttg aagagtttat
     2041 taatgaacca ctgaatgagg ttctagaaat ggataaagat tatacttttt tcaaagtaga
     2101 aacagagaac aaattctctt ttatgacatg tccctttata ttgaatgctg tcacaaagaa
     2161 tttgggatta tattatgaca atagaattcg catgtacagt gaacgaagaa tcactgttct
     2221 ctacagctta gttcaaggac agcagttgaa tccatatttg agactcaaag ttagacgtga
     2281 ccatatcata gatgatgcac ttgtccggct agagatgatc gctatggaaa atcctgcaga
     2341 cttgaagaag cagttgtatg tggaatttga aggagaacaa ggagttgatg agggaggtgt
     2401 ttccaaagaa ttttttcagc tggttgtgga ggaaatcttc aatccagata ttggtatgtt
     2461 cacatacgat gaatctacaa aattgttttg gtttaatcca tcttcttttg aaactgaggg
     2521 tcagtttact ctgattggca tagtactggg tctggctatt tacaataact gtatactgga
     2581 tgtacatttt cccatggttg tctacaggaa gctaatgggg aaaaaaggaa cttttcgtga
     2641 cttgggagac tctcacccag ttctatatca gagtttaaaa gatttattgg agtatgaagg
     2701 gaatgtggaa gatgacatga tgatcacttt ccagatatca cagacagatc tttttggtaa
     2761 cccaatgatg tatgatctaa aggaaaatgg tgataaaatt ccaattacaa atgaaaacag
     2821 gaaggaattt gtcaatcttt attctgacta cattctcaat aaatcagtag aaaaacagtt
     2881 caaggctttt cggagaggtt ttcatatggt gaccaatgaa tctcccttaa agtacttatt
     2941 cagaccagaa gaaattgaat tgcttatatg tggaagccgg aatctagatt tccaagcact
     3001 agaagaaact acagaatatg acggtggcta taccagggac tctgttctga ttagggagtt
     3061 ctgggaaatc gttcattcat ttacagatga acagaaaaga ctcttcttgc agtttacaac
     3121 gggcacagac agagcacctg tgggaggact aggaaaatta aagatgatta tagccaaaaa
     3181 tggcccagac acagaaaggt tacctacatc tcatacttgc tttaatgtgc ttttacttcc
     3241 ggaatactca agcaaagaaa aacttaaaga gagattgttg aaggccatca cgtatgccaa
     3301 aggatttggc atgctgtaaa acaaaacaaa acaaaataaa acaaaaaaaa ggaaggaaaa
     3361 aaaaagaaaa aatttaaaaa attttaaaaa tataacgagg gataaatttt tggtggtgat
     3421 agtgtcccag tacaaaaagg ctgtaagata gtcaaccaca gtagtcacct atgtctgtgc
     3481 ctcccttctt tattggggac atgtgggctg gaacagcaga tttcagctac atatatgaac
     3541 aaatccttta ttattattat aattattttt ttgcgtgaaa gtgttacata ttctttcact
     3601 tgtatgtaca gagaggtttt tctgaatatt tattttaagg gttaaatcac ttttgcttgt
     3661 gtttattact gcttgaggtt gagccttttg agtatttaaa aaatatatac caacagaact
     3721 actctcccaa ggaaaatatt gccaccattt gtagaccacg taaccttcaa gtatgtgcta
     3781 cttttttgtc cctgtatcta actcaaatca ggaactgtat tttttttaat gatttgcttt
     3841 tgaaacttga agtcttgaaa acagtgtgat gcaattactg ctgttctagc ccccaaagag
     3901 ttttctgtgc aaaatcttga gaatcaatca ataaagaaag atggaaggaa gggagaaatt
     3961 ggaatgtttt aactgcagcc ctcagaactt tagtaacagc acaacaaatt aaaaacaaaa
     4021 acaactcatg ccacagtatg tcgtcttcat gtgtcttgca atgaactgtt tcagtagcca
     4081 atcctctttc ttagtatatg aaaggacagg gatttttgtt cttgttgttc tcgttgttgt
     4141 tttaagttta ctggggaaag tgcatttggc caaatgaaat ggtagtcaag cctattgcaa
     4201 caaagttagg aagtttgttg tttgtttatt ataaacaaaa agcatgtgaa agtgcactta
     4261 agatagagtt tttattaatt acttacttat tacctagatt ttaaatagac aatccaaagt
     4321 ctccccttcg tgttgccatc atcttgttga atcagccatt ttatcgaggc acgtgatcag
     4381 tgttgcaaca taatgaaaaa gatggctact gtgccttgtg ttacttaatc atacagtaag
     4441 ctgacctgga aatgaatgaa actattactc ctaagaatta cattgtatag ccccacagat
     4501 taaatttaat taattaattc aaaacatgtt aaacgttact ttcatgtact atggaaaagt
     4561 acaagtaggt ttacattact gatttccaga agtaagtagt ttcccctttc ctagtcttct
     4621 gtgtatgtga tgttgttaat ttcttttatt gcattataaa ataaaaggat tatgtatttt
     4681 taactaaggt gagacattga tatatccttt tgctacaagc tatagctaat gtgctgagct
     4741 tgtgccttgg tgattgattg attgattgac tgattgtttt aactgattac tgtagatcaa
     4801 cctgatgatt tgtttgtttg aaattggcag gaaaaatgca gctttcaaat cattgggggg
     4861 agaaaaagga tgtctttcag gattatttta attaattttt ttcataattg agacagaact
     4921 gtttgttatg taccataatg ctaaataaaa ctgtggcact tttcaccata atttaattta
     4981 gtggaaaaag aagacaatgc tttccatatt gtgataaggt aacatggggt ttttctgggc
     5041 cagcctttag aacactgtta gggtacatac gctaccttga tgaaagggac cttcgtgcaa
     5101 ctgtagtcat cttaaaggct tctcatccac tgtgcttctt aatgtgtaat taaagtgagg
     5161 agaaattaaa tactctgagg gcgttttata taataaattc gtgaagaaat gtgtgctctt
     5221 cagttctcaa gttttattat tatggtattt attaaagttc tacaattgta ataacgtatc
     5281 catatgacaa gttttaaagt ggtaattgaa ataggttatc agatatagag ttgttcacat
     5341 caagtagact tttaacagag tcagaatgaa cctaccctta aaattttaga gaaaaaaaat
     5401 cgtcggttgc acagaatagc tgctctagct tgcttaatta tgccgggcat gttgtcactc
     5461 ctcttacttt tgctgccttt tcattactat ttaatggaat gtccctgaac aataaggaag
     5521 agcaaaacat agacattttg actacagtgg atacttcctc taccccaaat gttatgttat
     5581 aaaagtactt tttttgccca ggtactctat tatatatttt ggttttcttt gaattagacc
     5641 tcaatctcca ggaagctctg gagggaaaaa aaggaaccat aaactaaagt aactggtttt
     5701 ccaaataaat gtaaactttt ttaacctttt attattatag aacatttcaa acatacataa
     5761 aacatgaaaa cagcctgcag ccaaactttt ctggaccttg gcagctccag caaatgagct
     5821 ggtcatccta accctggttt cctgagacaa ctacttttgg ggatgcttgc ttgtataggg
     5881 agatgataga tagatagata catatcaaaa agaaacttga aaaaggcttc gaacagaact
     5941 tgccgatgtt actcatccct cctcggtaca gacattctgt tagaacagat ggttgtaatg
     6001 gggcagcatc actccctatt cccattaaaa acatgaccat tctgagattt caggcccctt
     6061 cgaggaattt ttctcaagaa ttccatacaa actgtcagag gaagaaatgt cgtcccaaga
     6121 ctagtccctg tgtagtcttg atccctttac ctgcttactg taattttttt tctctgactt
     6181 tccgccagtg tctaagggcc atctgtaccc ctcccaccct tccatgagcc actgtataat
     6241 ccaaaaacac accttccatt tgctccctca cttgtctcct tttacctcaa cagtgttagt
     6301 gtgtaaagaa ttgcctgggg agggtagagg gttttacaag tacagagtcc caagtctttt
     6361 ctcccctccc cgttagtggg ttggcattta ttaataggca ctgcaagatt ttgatgcagg
     6421 tgtggggtag atatcactgt gaaaggctac taatgcatgg tagtttcacc gaaagtacag
     6481 tcttcaagga tccttataat ctcctcattg ccaaaatcag taacatctat aaccttgaca
     6541 acaccctagc ttgggctgtt gcttctctgg ctacttgttt ttccatccca gtttacccag
     6601 attttagatg gatactcctc caattctgct taaagtcctt acctgttctt gcccagttcc
     6661 ctctagtcaa cctcagtcac tcccaaagat tcttccccaa tcccatatct tttgctcaga
     6721 cctctgtctc tgggatttct ttgttacctc atagtcagta gtcctaaaaa caaaattctt
     6781 catcttgcat ctctatcgac cgaccattct ttgtttcttt ttaagaaatg taaccttcac
     6841 cccaatcatc aaaatctctc attcctcaat tgtcacaggt tgtcacttct ttccccctaa
     6901 acctctccca tcattccttc cttctttata ctgcccttac aggtcctgat cctctgactc
     6961 acccggacat tcttagcagc tgcctaattt cactccctga ctctcatctc cctcttccaa
     7021 tccttttcat tgtgctagaa cactatcttg ctaattttaa tatcctctgc attcagtgcc
     7081 tccacagatt gccccctcac tgacctttcc aatttggcct tgtgttacac actttcctcg
     7141 ctgcattcct acccagcatc ttcacttgtc atatttctca aatactcttc atgaccagat
     7201 atttaaagta cctcatccat gaagacttca tcaaccccca aagtgcctcc aatgcttccg
     7261 tggtagcaca gcataatggg gtacatttct tagttgaaca gtgaggagtc actgggattc
     7321 ttacagagtg tcttagggtc tcctcttgat actctttcca catttcagtt aaggaatttt
     7381 cttacatctt tttttcttct aataaccttt gcctggagtg taagtaaatt catgatagag
     7441 actgtcattt ttaaaaaaaa aaacaaacca ttttgccttt cacatgttat tacctccatc
     7501 tttctgttat cttgtcagtg ttgccaccta aatgattgaa aatgaccgag catgcagtag
     7561 aatgtagtga agtttcctat ttcatccatt ttttgcataa gtgcattggt atctgtatat
     7621 ccatctgccc aagcaccagt tgttgaaaag actattattt ccccatgaat ggtcctggta
     7681 cccttgtcaa agaacagttg accataaata taagactttc tggattctca gtcttactcc
     7741 attgccctat atgtctgtcg ttataccagc accgcactgt cttgatggct gtagcttggt
     7801 agtaagttta gatttgggat gtgcaagtcc tccatttttt tttttttttt ccccaaaatc
     7861 attttgtctg ttttgggtcc cttgcattgc atatgaattt ttatatgatc attgtgtcaa
     7921 ttcctgcaaa acagctggga tcttgatagg gatttagttg aattggtaga taaatttgag
     7981 aaataactgc tattttaatg gtgttaggtt ttctgttcca tgactgtggg atgtctttgc
     8041 atttgcttag gtctttattt cagtgatgtt ttagagattt cagtgtgcaa gtcttgacgt
     8101 ctttaatttt tttctaagta ttttactgat gccactttaa ttatatgatt attttctaat
     8161 ttttattttt gtattcatca ctggtgttag aaataaaatt aatttttgta tattgatttt
     8221 gtatattgta gcttttctaa aattcttttt ttgtcactta gtattttttg catatgagat
     8281 catgcatctg tgaacagaaa tacttttact tcttcctttc cagtctagat tcctattatt
     8341 tcattttctt gccttagtgt gctggctagc acctccagta caatattgga tagaagtgac
     8401 aagagcagac atctttttct tgttcctgat tttaagggga aagcattcag tctttcacca
     8461 ttgagtatga tgttagctag ctgtgggttt gcttcaagtt gaggaaattc ttttattaaa
     8521 cctagtttac tgagtgtttt ttaatcaaaa aaaaaaaaag gaggggctgt gggttttgtt
     8581 aaatgcttct tctgtatcaa gattttcatg ttgttttgtc ctttattaat atgttgtatt
     8641 aatggacttt tgtatattga attaactttg cattcctggg ataatctcag ttgtccatgc
     8701 tacataaacc ttgttctatg ctgctgga
//
