LOCUS       NM_017811               4477 bp    mRNA    linear   PRI 12-DEC-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 R2 (UBE2R2), mRNA.
ACCESSION   NM_017811
VERSION     NM_017811.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Welsh,K.A., Bolhuis,D.L., Nederstigt,A.E., Boyer,J., Temple,B.R.S.,
            Bonacci,T., Gu,L., Ordureau,A., Harper,J.W., Steimel,J.P.,
            Zhang,Q., Emanuele,M.J., Harrison,J.S. and Brown,N.G.
  TITLE     Functional conservation and divergence of the helix-turn-helix
            motif of E2 ubiquitin-conjugating enzymes
  JOURNAL   EMBO J 41 (3), e108823 (2022)
   PUBMED   34942047
  REMARK    GeneRIF: Functional conservation and divergence of the
            helix-turn-helix motif of E2 ubiquitin-conjugating enzymes.
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 4477)
  AUTHORS   Hill,S., Reichermeier,K., Scott,D.C., Samentar,L.,
            Coulombe-Huntington,J., Izzi,L., Tang,X., Ibarra,R., Bertomeu,T.,
            Moradian,A., Sweredoski,M.J., Caberoy,N., Schulman,B.A.,
            Sicheri,F., Tyers,M. and Kleiger,G.
  TITLE     Robust cullin-RING ligase function is established by a multiplicity
            of poly-ubiquitylation pathways
  JOURNAL   Elife 8, e51163 (2019)
   PUBMED   31868589
  REMARK    GeneRIF: UBE2R2 alone had negligible ubiquitylation activity at
            physiological concentrations and the ablation of UBE2R1/2 had no
            effect on the stability of SCF substrates in cells. A genome-wide
            CRISPR screen revealed that an additional E2 enzyme, UBE2G1,
            buffers against the loss of UBE2R1/2.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4477)
  AUTHORS   Hill,S., Harrison,J.S., Lewis,S.M., Kuhlman,B. and Kleiger,G.
  TITLE     Mechanism of Lysine 48 Selectivity during Polyubiquitin Chain
            Formation by the Ube2R1/2 Ubiquitin-Conjugating Enzyme
  JOURNAL   Mol Cell Biol 36 (11), 1720-1732 (2016)
   PUBMED   27044868
  REMARK    GeneRIF: Study shows that Ube2R1/2 forms a salt bridge interaction
            between a conserved Asp residue on Ube2R1/2 and acceptor ubiquitin
            residue Arg 54 and that perturbation of this interaction leads to
            the severe loss of UbeR2 activity. Results also provide new insight
            into how the Ube2R1/2 acidic loop may participate in catalysis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4477)
  AUTHORS   Yoshida,T., Kato,K., Yokoi,K., Oguri,M., Watanabe,S., Metoki,N.,
            Yoshida,H., Satoh,K., Aoyagi,Y., Nozawa,Y. and Yamada,Y.
  TITLE     Association of genetic variants with hemorrhagic stroke in Japanese
            individuals
  JOURNAL   Int J Mol Med 25 (4), 649-656 (2010)
   PUBMED   20198315
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 4477)
  AUTHORS   David,Y., Ziv,T., Admon,A. and Navon,A.
  TITLE     The E2 ubiquitin-conjugating enzymes direct polyubiquitination to
            preferred lysines
  JOURNAL   J Biol Chem 285 (12), 8595-8604 (2010)
   PUBMED   20061386
REFERENCE   7  (bases 1 to 4477)
  AUTHORS   Oguri,M., Kato,K., Yokoi,K., Yoshida,T., Watanabe,S., Metoki,N.,
            Yoshida,H., Satoh,K., Aoyagi,Y., Nozawa,Y. and Yamada,Y.
  TITLE     Assessment of a polymorphism of SDK1 with hypertension in Japanese
            Individuals
  JOURNAL   Am J Hypertens 23 (1), 70-77 (2010)
   PUBMED   19851296
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 4477)
  AUTHORS   Humphray,S.J., Oliver,K., Hunt,A.R., Plumb,R.W., Loveland,J.E.,
            Howe,K.L., Andrews,T.D., Searle,S., Hunt,S.E., Scott,C.E.,
            Jones,M.C., Ainscough,R., Almeida,J.P., Ambrose,K.D., Ashwell,R.I.,
            Babbage,A.K., Babbage,S., Bagguley,C.L., Bailey,J., Banerjee,R.,
            Barker,D.J., Barlow,K.F., Bates,K., Beasley,H., Beasley,O.,
            Bird,C.P., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.,
            Burrill,W., Burton,J., Carder,C., Carter,N.P., Chapman,J.C.,
            Chen,Y., Clarke,G., Clark,S.Y., Clee,C.M., Clegg,S., Collier,R.E.,
            Corby,N., Crosier,M., Cummings,A.T., Davies,J., Dhami,P., Dunn,M.,
            Dutta,I., Dyer,L.W., Earthrowl,M.E., Faulkner,L., Fleming,C.J.,
            Frankish,A., Frankland,J.A., French,L., Fricker,D.G., Garner,P.,
            Garnett,J., Ghori,J., Gilbert,J.G., Glison,C., Grafham,D.V.,
            Gribble,S., Griffiths,C., Griffiths-Jones,S., Grocock,R., Guy,J.,
            Hall,R.E., Hammond,S., Harley,J.L., Harrison,E.S., Hart,E.A.,
            Heath,P.D., Henderson,C.D., Hopkins,B.L., Howard,P.J., Howden,P.J.,
            Huckle,E., Johnson,C., Johnson,D., Joy,A.A., Kay,M., Keenan,S.,
            Kershaw,J.K., Kimberley,A.M., King,A., Knights,A., Laird,G.K.,
            Langford,C., Lawlor,S., Leongamornlert,D.A., Leversha,M., Lloyd,C.,
            Lloyd,D.M., Lovell,J., Martin,S., Mashreghi-Mohammadi,M.,
            Matthews,L., McLaren,S., McLay,K.E., McMurray,A., Milne,S.,
            Nickerson,T., Nisbett,J., Nordsiek,G., Pearce,A.V., Peck,A.I.,
            Porter,K.M., Pandian,R., Pelan,S., Phillimore,B., Povey,S.,
            Ramsey,Y., Rand,V., Scharfe,M., Sehra,H.K., Shownkeen,R.,
            Sims,S.K., Skuce,C.D., Smith,M., Steward,C.A., Swarbreck,D.,
            Sycamore,N., Tester,J., Thorpe,A., Tracey,A., Tromans,A.,
            Thomas,D.W., Wall,M., Wallis,J.M., West,A.P., Whitehead,S.L.,
            Willey,D.L., Williams,S.A., Wilming,L., Wray,P.W., Young,L.,
            Ashurst,J.L., Coulson,A., Blocker,H., Durbin,R., Sulston,J.E.,
            Hubbard,T., Jackson,M.J., Bentley,D.R., Beck,S., Rogers,J. and
            Dunham,I.
  TITLE     DNA sequence and analysis of human chromosome 9
  JOURNAL   Nature 429 (6990), 369-374 (2004)
   PUBMED   15164053
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL139113.21, CD515389.1,
            BC047584.1, AK000426.1, BC004862.2, CN427315.1, AL133827.1,
            BQ432410.1, BI908167.1, CN310827.1, BI061548.1, AA397839.1 and
            AW966809.1.
            
            On Nov 22, 2018 this sequence version replaced NM_017811.3.
            
            Summary: Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr
            protein kinase involved in cell growth and transformation. This
            gene encodes a protein similar to the E2 ubiquitin conjugating
            enzyme UBC3/CDC34. Studies suggest that CK2-dependent
            phosphorylation of this ubiquitin-conjugating enzyme functions by
            regulating beta-TrCP substrate recognition and induces its
            interaction with beta-TrCP, enhancing beta-catenin degradation.
            [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.145939.1,
                                           SRR1660807.251342.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263228.4/ ENSP00000263228.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AL139113.21        20463-20486
            25-168              CD515389.1         23-166
            169-459             BC047584.1         1-291
            460-712             AK000426.1         53-305
            713-1435            BC047584.1         545-1267
            1436-1577           BC004862.2         1019-1160
            1578-1967           AK000426.1         1171-1560
            1968-2068           BC047584.1         1799-1899
            2069-2483           CN427315.1         226-640
            2484-2653           AL133827.1         584-753
            2654-3085           BQ432410.1         256-687
            3086-3391           BI908167.1         340-645
            3392-3746           CN310827.1         345-699
            3747-3899           BI061548.1         359-511
            3900-4114           AA397839.1         228-442
            4115-4477           AW966809.1         163-525
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.3"
     gene            1..4477
                     /gene="UBE2R2"
                     /gene_synonym="CDC34B; E2-CDC34B; UBC3B"
                     /note="ubiquitin conjugating enzyme E2 R2"
                     /db_xref="GeneID:54926"
                     /db_xref="HGNC:HGNC:19907"
                     /db_xref="MIM:612506"
     exon            1..775
                     /gene="UBE2R2"
                     /gene_synonym="CDC34B; E2-CDC34B; UBC3B"
                     /inference="alignment:Splign:2.1.0"
     CDS             599..1315
                     /gene="UBE2R2"
                     /gene_synonym="CDC34B; E2-CDC34B; UBC3B"
                     /EC_number="2.3.2.23"
                     /note="ubiquitin-conjugating enzyme UBC3B;
                     ubiquitin-protein ligase R2; ubiquitin carrier protein R2;
                     ubiquitin conjugating enzyme E2R 2; ubiquitin-conjugating
                     enzyme E2-CDC34B; E2 ubiquitin-conjugating enzyme R2"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 R2"
                     /protein_id="NP_060281.2"
                     /db_xref="CCDS:CCDS6546.1"
                     /db_xref="GeneID:54926"
                     /db_xref="HGNC:HGNC:19907"
                     /db_xref="MIM:612506"
                     /translation="MAQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVA
                     IFGPPNTLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVD
                     DPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKE
                     YAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDLYDDDIDDE
                     DEEEEDADCYDDDDSGNEES"
     exon            776..862
                     /gene="UBE2R2"
                     /gene_synonym="CDC34B; E2-CDC34B; UBC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            863..960
                     /gene="UBE2R2"
                     /gene_synonym="CDC34B; E2-CDC34B; UBC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1095
                     /gene="UBE2R2"
                     /gene_synonym="CDC34B; E2-CDC34B; UBC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..4477
                     /gene="UBE2R2"
                     /gene_synonym="CDC34B; E2-CDC34B; UBC3B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2043..2048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2R2"
                     /gene_synonym="CDC34B; E2-CDC34B; UBC3B"
                     /note="hexamer: AATAAA"
     polyA_site      2068
                     /gene="UBE2R2"
                     /gene_synonym="CDC34B; E2-CDC34B; UBC3B"
                     /note="major polyA site"
ORIGIN      
        1 gcttctcggc tccgttgctc ccgcggcgcg aggcgctctc gctctcctcc tcctccgccg
       61 tcgcccctcc ccccgcgcag cccccgccgc caccgccgcg agacccccgc acgccgctct
      121 ccccccaccc tctcctgccc cgcgcgccct ccggcccctg cggcccccga agagaacgag
      181 agggcgagcg agcgcggcgt tcccgggccg gcccggcccc ctcccttcac catcgccggc
      241 cctccgccgc cttcctccgc ttccacctcc tctccccccc cccaagttga ggccccctcc
      301 ggggggggga ggcccgggac cgggacccgt agcgaattct cctaactccc tcgacctctc
      361 ctctcgcccg gcccgagtgt gaggagaagg gcccggcccg gcctgcgtcg tgtgtgagga
      421 ggaccccggg cgggcccacg ggccgtgtgg ggcctggtct ggcccgccgg gtgtgtgaag
      481 accggggccc ggtgctgccc ggccggaggg cgagcggagg ggaggggcct ggtccggccc
      541 ggccggtgcg tgaggactgg ggcccgggcc cggcgccgcc gccgccgccg ccgccgcgat
      601 ggcccagcag cagatgacca gctcgcagaa ggccctgatg ctcgagctga aatccctgca
      661 ggaggaaccg gtggagggct tccggatcac cctggtggac gagtccgacc tctacaactg
      721 ggaggtggcc atcttcggac cccccaacac cctctacgaa ggcggctact tcaaggcgca
      781 tattaaattt cctattgact acccctattc accacctacc ttcagattct tgaccaaaat
      841 gtggcacccc aacatttatg agaatggaga tgtatgcatt tcgattcttc atccgcctgt
      901 agatgaccca cagagtggag aactgccttc tgaaaggtgg aatcctactc agaatgtgag
      961 gactatccta ttaagtgtaa tctcactgct taatgagccc aacaccttct ccccagccaa
     1021 tgtcgatgct tcagttatgt tcaggaaatg gagagacagt aaaggaaaag acaaagaata
     1081 tgctgaaatt attaggaaac aagtttcagc cactaaggcc gaagcagaaa aggatggagt
     1141 gaaggtcccc acaaccctgg cggaatactg catcaaaact aaagtgcctt ccaatgacaa
     1201 cagctcagat ttgctttacg acgacttgta tgatgacgac attgatgatg aagatgagga
     1261 ggaggaagat gccgactgtt atgatgatga tgattctggg aatgaggagt cgtgacgtgc
     1321 tccttcagtg cccctgtact gccctgccat ctcaggccaa agggagggga gcaagtgggg
     1381 acctggccat ggcccctcag caaaaaccta ttcacagcgg gtggggaaac acacacagct
     1441 cctgctgact ccccttatgg atctcagttt gctccttttt atggaccttt aatggagaga
     1501 gagtaaccct ccacagaatg tctgaattct tgcattcttt acccttccat cactatattg
     1561 attctttttt taaaaaatat gaacccaaac tcccgcctca cttcgtctct acagaatgtt
     1621 cacagcaaaa cacgtttggt ctgtttttag attcttgaag aattcaatag tctttcaaga
     1681 tgtttaatgt gtttaaagct gggaacctgt tgggagttca caagtgctgc atatactggg
     1741 tagcaaaaga aaatggaaaa aaacccacaa aacaaacttt aaaaaaaaaa aaaaacaaat
     1801 ttgccaaggt ttagctgctc atttacatta gtgtgtgtgc attcgttcag ccccatggtg
     1861 gtgaattctg tttctttcct ttcctaaggc tgggacatgg tgggcatcag ggactttgtg
     1921 ctaagcctga tgaaatgtgc tccttcaatc tccatgaaac catcgtaaca tggaggcctc
     1981 agctgctctg aggagagaaa tcagactttg ttttttgaaa tcgattggga tcgaaagcct
     2041 gaaataaata ttcatacttt ccatagaact gagcacttgg ttgctattta ttttaaaggc
     2101 agggttattt ccccccaggg agtggggcaa gcggggaagt tgggatgagt gcgtgtggga
     2161 gcaaagagtt aaaaatcact atgctaagtt tggttgatgc tactgggggg aaaaagttga
     2221 aactactggt gaccactgtt gggagaggaa acatctgttt tatagattta gcatggcctt
     2281 cccatcaaaa atacactcgt accatgacgt tttgtttttg ttttttaagt agccactttt
     2341 aattactcag tattaatcct aggtgcatgt gttaagattt cggttttcat cgagctgctt
     2401 atactgatag tgaaaaactt ggatgtgtgt gagacgagtt accgccttct tcattggatg
     2461 tgatctgaac tttttttctc ctttcaacat acttaggctg tggagtgcaa gcagaattat
     2521 gcatagaaat tgtggcaggg ctgagggagt tgactgcagg cagtttttag gccagataag
     2581 gctagatctt cctgcaatgc acttgctgca gctttgattt tccagatctc aatcgtgttg
     2641 cttcaatcaa tattgagatc cagcagcctt caccatccag gagacttcag aacttgaagg
     2701 taatttttgg ttgttatggg ggtgcaccag atcataggag ggcattattt ggaggaaacc
     2761 gctataatgt aaaatcagat ttcagaagga aaatgcagca aggactgact gtagtcctat
     2821 ttcagacccc tgggcactag tgtcaactcc tcctttggct cgtaactcag aaagaaccaa
     2881 agggcagatt agggacgggg caggagggat cctaggatgg ccagagtagg tgacgcttac
     2941 ttccttagct cctccttcca ttctgatctc agggttttca ctcttcaaac tcccaaacac
     3001 atgacttctt cccagcagcc cttcccggcc tctaaagttt ggtcccagca gcccttccca
     3061 gcctctaaag tttggtctgg tatgaaatgg gactgtttaa caagcaaggc tcttagggga
     3121 cactgttgag cctggtccca gaatctgtga tctccacagg gccagggttg ctacttgcac
     3181 ccagaatcta gtgattttag tcagatccat ggaacagagc agcttcgtaa tacattgtca
     3241 agttacctga ttacaaactc tcatagatgt tggaactacc tttttactcc ccatactttg
     3301 taagtaatgc ttccagatta acctgcaaat cttgtggagc caaagcctct cacagtgctg
     3361 atttacctcc cccaaaatgc ccccaaaccc ccatgcaagt ttacagccag tagcttggtc
     3421 ttattctctt ggagccctaa aattaagcct ttcattcccc tttgctaaag catctcactc
     3481 ccagggcagc cccagcaccc tggcattgcg ggaggtgctc tgctgcacag ctgttccatc
     3541 aggccctatt ggcctctgtt agacattgac tcagggagcc ggagcgccaa gtggaggttg
     3601 tacctttcag ccctttgggg tgttaaaagt gtcaagggct gggtttgcct ctgatgagta
     3661 caatcagttc cagaagactg ggtaagtctc ttgctagcag gtggacattc tggtatttta
     3721 gactgactga acgaaaccca gaggctggtt ccagagctct gtccctgaga acacatccct
     3781 gtggaaaata cttgagtttg taattttttt tcccccttct gggagtggag gtctcaggct
     3841 caatccggaa ccatgtacgt acagttttgc ttattatttg atgtgacatg aactcaaaat
     3901 cctgcatttt tagattcctt ttagttctaa ttgaggcctc actagatgct cttcagttct
     3961 gtgtattttg aggctgggtg gagaagtata tgattctgct gttgattctt tcagtcttcc
     4021 ccccttctcc ttttatcaat cattccagag ttattttcta ttagtcaaac ttttttgtag
     4081 aaccttcttt cccttaccct ggcaaattgc ttctttactt gctggtttcc ttacgtttgg
     4141 ggcacatgtt gtaagaaact gattggaagg ggaaatgtgc agctctccac tggaaaggaa
     4201 ctctccaccc ctcccatcct gataaaacaa acaaggttta catttacaac taaaaggatt
     4261 cagatgcaat tttcaactat tctgaaacca gcaggacaca cctgacttta atagttttag
     4321 ctgaaattgt agatgttttg cttcagttta acttatgaga aaagattatc tgacggattt
     4381 tgtgttgact tcccctttag tggtttattt tgtctttttc tgcccatctg tctactaata
     4441 aaatgtgaaa taaaatacct gtattgctac ttcccca
//
