LOCUS       NM_022066               5377 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.
ACCESSION   NM_022066
VERSION     NM_022066.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Lan,S.Y., Ding,Y., Wang,C., Fang,J., Ren,C., Liu,J.L., Kang,H. and
            Chang,Y.
  TITLE     High Level of Ubiquitin Conjugate Enzyme E2O Indicates Poor
            Prognosis of Patients with Hepatocellular Carcinoma
  JOURNAL   Curr Med Sci 43 (1), 93-103 (2023)
   PUBMED   36269535
  REMARK    GeneRIF: High Level of Ubiquitin Conjugate Enzyme E2O Indicates
            Poor Prognosis of Patients with Hepatocellular Carcinoma.
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Cheng,J., Zheng,H., Liu,C., Jin,J., Xing,Z. and Wu,Y.
  TITLE     Age-Associated UBE2O Reduction Promotes Neuronal Death in
            Alzheimer's Disease
  JOURNAL   J Alzheimers Dis 93 (3), 1083-1093 (2023)
   PUBMED   37182872
  REMARK    GeneRIF: Age-Associated UBE2O Reduction Promotes Neuronal Death in
            Alzheimer's Disease.
REFERENCE   3  (bases 1 to 5377)
  AUTHORS   Yip,M.C.J., Sedor,S.F. and Shao,S.
  TITLE     Mechanism of client selection by the protein quality-control factor
            UBE2O
  JOURNAL   Nat Struct Mol Biol 29 (8), 774-780 (2022)
   PUBMED   35915257
  REMARK    GeneRIF: Mechanism of client selection by the protein
            quality-control factor UBE2O.
REFERENCE   4  (bases 1 to 5377)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 5377)
  AUTHORS   Huang,Q., Qin,D., Pei,D., Vermeulen,M. and Zhang,X.
  TITLE     UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and homologous
            recombination-mediated DNA repair
  JOURNAL   FASEB J 36 (1), e22112 (2022)
   PUBMED   34921745
  REMARK    GeneRIF: UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and
            homologous recombination-mediated DNA repair.
REFERENCE   6  (bases 1 to 5377)
  AUTHORS   Davila,S., Froeling,F.E., Tan,A., Bonnard,C., Boland,G.J.,
            Snippe,H., Hibberd,M.L. and Seielstad,M.
  TITLE     New genetic associations detected in a host response study to
            hepatitis B vaccine
  JOURNAL   Genes Immun 11 (3), 232-238 (2010)
   PUBMED   20237496
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 5377)
  AUTHORS   Markson,G., Kiel,C., Hyde,R., Brown,S., Charalabous,P., Bremm,A.,
            Semple,J., Woodsmith,J., Duley,S., Salehi-Ashtiani,K., Vidal,M.,
            Komander,D., Serrano,L., Lehner,P. and Sanderson,C.M.
  TITLE     Analysis of the human E2 ubiquitin conjugating enzyme protein
            interaction network
  JOURNAL   Genome Res 19 (10), 1905-1911 (2009)
   PUBMED   19549727
REFERENCE   8  (bases 1 to 5377)
  AUTHORS   Pope,S.N. and Lee,I.R.
  TITLE     Yeast two-hybrid identification of prostatic proteins interacting
            with human sex hormone-binding globulin
  JOURNAL   J Steroid Biochem Mol Biol 94 (1-3), 203-208 (2005)
   PUBMED   15862967
REFERENCE   9  (bases 1 to 5377)
  AUTHORS   Tomsig,J.L., Snyder,S.L. and Creutz,C.E.
  TITLE     Identification of targets for calcium signaling through the copine
            family of proteins. Characterization of a coiled-coil
            copine-binding motif
  JOURNAL   J Biol Chem 278 (12), 10048-10054 (2003)
   PUBMED   12522145
REFERENCE   10 (bases 1 to 5377)
  AUTHORS   Yokota,T., Nagai,H., Harada,H., Mine,N., Terada,Y., Fujiwara,H.,
            Yabe,A., Miyazaki,K. and Emi,M.
  TITLE     Identification, tissue expression, and chromosomal position of a
            novel gene encoding human ubiquitin-conjugating enzyme E2-230k
  JOURNAL   Gene 267 (1), 95-100 (2001)
   PUBMED   11311559
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB051521.1, DA092358.1, BC025977.1 and AC090699.10.
            
            On Nov 23, 2018 this sequence version replaced NM_022066.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.9431.1,
                                           SRR1660809.204905.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000319380.12/ ENSP00000323687.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-159               AB051521.1         55-213
            160-273             DA092358.1         158-271
            274-3629            AB051521.1         328-3683
            3630-5301           BC025977.1         1083-2754
            5302-5377           AC090699.10        101482-101557       c
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.1"
     gene            1..5377
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /note="ubiquitin conjugating enzyme E2 O"
                     /db_xref="GeneID:63893"
                     /db_xref="HGNC:HGNC:29554"
                     /db_xref="MIM:617649"
     exon            1..428
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..3890
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /EC_number="2.3.2.24"
                     /note="ubiquitin-protein ligase O; ubiquitin conjugating
                     enzyme E2O; ubiquitin carrier protein O;
                     ubiquitin-conjugating enzyme E2 of 230 kDa;
                     ubiquitin-conjugating enzyme E2-230K; E2/E3 hybrid
                     ubiquitin-protein ligase UBE2O"
                     /codon_start=1
                     /product="(E3-independent) E2 ubiquitin-conjugating
                     enzyme"
                     /protein_id="NP_071349.3"
                     /db_xref="CCDS:CCDS32742.1"
                     /db_xref="GeneID:63893"
                     /db_xref="HGNC:HGNC:29554"
                     /db_xref="MIM:617649"
                     /translation="MADPAAPTPAAPAPAQAPAPAPEAVPAPAAAPVPAPAPASDSAS
                     GPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCS
                     EAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTD
                     SQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKN
                     QIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSV
                     QWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLG
                     RVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKK
                     QVVRIMSCSPDTQCSRDHSMEDPDKKGESKTKSEAESASPEETPDGSASPVEMQDEGA
                     EEPHEAGEQLPPFLLKEGRDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGS
                     GTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVEC
                     NIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKL
                     RPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVA
                     RVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDS
                     WETDNGLVEDEHPKIEEPPIPPLEQPVAPEDKGVVISEEAATAAVQGAVAMAAPMAGL
                     MEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDD
                     IKKLQENLKKTLDNVAIVEEEKMEAVPDVERKEDKPEGQSPVKAEWPSETPVLCQQCG
                     GKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEG
                     IMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLN
                     PNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDR
                     GLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWL
                     ETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGG
                     AQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLS
                     KGFIKSIRGVLTQFRAALLEAGMPECTEDK"
     exon            429..488
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            489..599
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            600..697
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            698..761
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            762..905
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            906..1015
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1166
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1639
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            1640..1794
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..1907
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..2036
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2126
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            2127..2820
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            2821..2957
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            2958..3161
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            3162..3219
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     exon            3220..5377
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5280..5285
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /note="hexamer: ATTAAA"
     polyA_site      5301
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /note="major polyA site"
     regulatory      5355..5360
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
                     /note="hexamer: ATTAAA"
     polyA_site      5377
                     /gene="UBE2O"
                     /gene_synonym="E2-230K"
ORIGIN      
        1 gcagagcagt tatggcggat cccgcagccc ccacgcccgc agctcccgct ccagcccagg
       61 ccccggctcc agccccggag gcagtcccgg ccccagccgc agcccccgtc ccggcgccgg
      121 cgcccgcctc ggactcggcc tccgggccgt cctcggactc cggcccagaa gccggctcgc
      181 agcgcctgct gttttctcac gacctggtgt cgggccgtta ccgtggctcc gtgcacttcg
      241 ggctggtgcg cctcatccac ggcgaggact cggactcgga gggcgaggag gagggccgcg
      301 ggagctcggg gtgctccgag gccgggggcg cgggccacga ggagggccgg gccagccccc
      361 tgcgccgcgg ctacgtgcgc gtccagtggt acccggaggg cgtcaagcag catgtgaagg
      421 agaccaagct gaaactagag gaccgttctg tggtgccccg agatgtggtc cggcacatgc
      481 gatccaccga cagtcagtgt ggcacggtga tcgacgtcaa catcgactgt gccgtcaagc
      541 tcatcggcac caactgcatc atctatcccg tcaacagcaa ggacctgcag cacatctggc
      601 ccttcatgta tggggactac attgcctatg actgctggct ggggaaggtc tacgacttga
      661 agaaccagat catcctgaag ctatccaacg gcgccaggtg ctccatgaac acggaagatg
      721 gcgccaagct ctacgacgtc tgcccgcacg tcagcgactc gggtctcttc ttcgatgatt
      781 cctatggctt ctacccaggc caggtgctca ttggccctgc caagatcttc tccagcgtcc
      841 agtggctgtc aggtgtcaag cccgtgctca gcaccaagag caagttccga gtggtggtgg
      901 aagaggtgca ggttgtagag ttgaaagtta catggattac caagagtttc tgtccagggg
      961 gcacggacag cgtcagcccc ccaccctctg tcatcaccca ggaaaaccta ggcagggtga
     1021 agcgtctcgg atgctttgac catgctcagc ggcagcttgg ggagcgctgt ctgtatgtct
     1081 tcccagccaa ggtagagcca gccaagattg cctgggaatg tccagaaaaa aactgcgccc
     1141 agggggaggg ctctatggcc aagaaggtga agcgcctgtt gaagaagcag gttgtgcgga
     1201 tcatgtcatg ctccccagac acccagtgtt cccgggacca ttccatggaa gacccagaca
     1261 agaaggggga atccaaaacc aagagcgaag cggagtctgc cagccctgag gagacgcccg
     1321 atggctctgc cagtccagtg gagatgcagg acgagggtgc agaggagccc cacgaggcag
     1381 gagagcagct gcccccattc ctgctaaaag aaggcagaga tgacaggctg cactcggcag
     1441 agcaggacgc agatgatgag gctgctgatg acacggacga caccagttcg gtgacctcct
     1501 ctgccagctc caccacttcc tcccagagcg gcagcggcac gagtcgcaaa aagagcatcc
     1561 ccttgtccat caagaactta aagcgcaaac acaagaggaa gaagaataaa atcactcgag
     1621 acttcaagcc aggggacagg gtggcagtgg aggtggtgac cacgatgacc tcagccgacg
     1681 tgatgtggca ggatggctcc gtggaatgca acatccgctc caacgacctc ttccctgtgc
     1741 accacctgga caacaacgag ttctgccctg gagacttcgt ggtagataag cgagtccaga
     1801 gctgtccaga ccctgctgtc tacggtgtgg tacagtctgg ggaccacatc ggccgtacct
     1861 gcatggtgaa gtggttcaag ctgaggccga gtggggacga cgtggagctg attggagaag
     1921 aggaagatgt gagtgtttac gacattgctg accaccctga ctttaggttc cgtacaactg
     1981 acatcgtcat ccgcatcggc aatactgagg atggggctcc tcacaaggag gatgagccat
     2041 cggtgggcca ggtggcccgt gtggacgtca gcagcaaggt ggaggtggtg tgggctgaca
     2101 actcaaagac catcatcctg ccccagcact tgtacaacat agagtctgag attgaggagt
     2161 cagactacga ttcggtagaa ggcagcacca gcggggcatc ctcggatgaa tgggaagatg
     2221 atagtgacag ctgggagacg gacaatgggc tggtggagga cgagcacccc aagatagagg
     2281 agccccccat cccacccctg gagcagccgg tggcccctga ggacaaggga gtggtgatca
     2341 gtgaagaggc agccacagct gccgtccagg gggctgtggc catggctgcc cccatggccg
     2401 ggctgatgga gaaggctggc aaggacgggc cacccaagag cttccgggag ttgaaagagg
     2461 ccatcaagat cctggagagc ctcaagaaca tgactgtgga gcagctgctg acgggctcgc
     2521 ccacctctcc gactgtggag cctgagaagc caactcggga gaagaagttt ctggatgaca
     2581 tcaagaagct acaggaaaac ctcaagaaga ccctggacaa tgtggccatt gtagaggagg
     2641 agaagatgga agcagtgccc gacgtagagc gcaaggagga caagcccgag gggcagtcac
     2701 ctgtgaaggc tgagtggccc agcgaaaccc cggtgctgtg ccagcagtgt ggcggcaagc
     2761 ctggcgtcac cttcaccagc gccaagggcg aggtcttctc cgtactggag tttgcaccct
     2821 caaatcattc ttttaagaaa attgagttcc agcctccaga agccaagaag ttcttcagca
     2881 cagtgcggaa ggagatggcg ctgctggcta cctcactgcc tgagggcatc atggtcaaga
     2941 cttttgaaga tagaatggac ctcttctcag ctctcatcaa gggccccact cgaaccccct
     3001 acgaggatgg cctctacttg tttgacatcc agctccccaa catctaccca gccgtgcccc
     3061 cccacttctg ctacctctcc caatgcagtg gccgcctgaa ccccaacctg tatgacaatg
     3121 ggaaggtgtg tgtcagcctc ctgggcacct ggattggaaa ggggacagag aggtggacaa
     3181 gcaagtccag ccttctccag gtgctcatct ccatccaagg tctgatcctg gtaaatgaac
     3241 catactacaa cgaagccggc ttcgacagtg accgaggcct gcaggaaggc tatgaaaaca
     3301 gtcgctgtta caatgagatg gcgctgatcc gcgtggtgca gtccatgacc cagctggtgc
     3361 ggcggccccc cgaggtcttt gagcaggaga tcaggcaaca ctttagcact ggtggctggc
     3421 ggctggtgaa ccgtatcgag tcctggctgg aaacccatgc cctgctggag aaggcccagg
     3481 cactgcccaa cggggtgccc aaggccagca gctcgccaga gcccccagct gtagccgagc
     3541 tgtcagactc cggccaacaa gaacctgagg atggagggcc agccccagga gaggcctccc
     3601 agggctcaga ctcagagggc ggtgcccagg gcctggcctc agctagcagg gaccacacag
     3661 accagacttc ggagaccgca ccagacgcat cggtgccacc cagtgtgaaa ccaaagaagc
     3721 ggagaaagag ctaccggagc ttcttacctg agaagagtgg ctaccctgac atcggcttcc
     3781 ccctcttccc actttccaag ggtttcatca agagcatccg gggtgtcctg acgcagttcc
     3841 gggctgccct gctagaggca ggcatgccgg agtgcacaga ggacaagtag ctgccaggca
     3901 cagaggaaag agcatcaccg tgggagaggc cagccgccgc ctgctcactc ccccccggaa
     3961 tcacccctct tcccatgccc ctctgtcccc actgcaaacc cactgccctc ttctccccaa
     4021 ggtgagtttg atgctgaagt gcaagaagtg tgttgagatg ctgccgtttc tattttgaag
     4081 cgagctttca acaggcgggt cccctgtggc aaagaaaatc ggaaccctgt tgccgatttt
     4141 ccatttgtca ccccagcaga atgtccggca cttgctccct tgctgcccct tctcaggtca
     4201 gaggcgggtg ttccagggcc tgccgcgggg ctctctgggc cggttccctg cagacccgca
     4261 ggagagcaca tgtgccttgc atgaagtgtg ggttgcgcca acaattcccc tggtcccttt
     4321 caacctgttt agttcaactc aagcctccct gtgtcccaga ccctcctgct gccaccacca
     4381 cccaggtcct ccctagtcct ccagcgtcaa cactatccct tgggagttgt agctgctgtc
     4441 actgactccc ggctatacat ggcctgtcga ccacgttata gccctcaggc ctgttgaact
     4501 tgctctctaa gagaggttgg gaccaggcta ggttccgggt gacgcccagg agaggtggtg
     4561 gccttcacac atgcacatgg agttgaggac cagggagctg cagggaaagc aacagctata
     4621 ggtgccttgc tcttctgtcg gaggctgctg ggggcaagag cagctgcaca aggccagggc
     4681 aagtgctagg gcccctcccc catcacatgg tcacactggg acaggcgtgc agctcactga
     4741 actccaagcg agccagccct ctcttggact agaaggccta ctgtcagccc ttcgcttaca
     4801 aactgcaggc tcaatccgaa ggggacggcc ggcgggggct ctcctagtgc ccagagacag
     4861 gcccagaggt ttacaagttt tctaagcttt tgataatgtg aagctccagg ccgagaggat
     4921 gctgttgagc acattgcagc tatgtaattt ttggtgtatg tatgtaatat ttaaggttgg
     4981 aaaaaaaact caaaagcaaa gatattaact cttattagaa aaaaagacaa aaaaaaagcc
     5041 aaagcatgat gcgtcttgtc agccttaagt gggctccaca cctgtgctgt gctgtgaccg
     5101 cccagccagc agagctgcgg gaggatggag ccggaccaca caccgtggca tttggaaccg
     5161 agtcggtatc ttgtttgaga aacacccgga gtgactggtg gggctgtgct tcccagtgca
     5221 ttgtacatgt ggagatgtga atgcctactg cttacgatat ctgtataaag tgctgtgtga
     5281 ttaaactttt ttttacttgc attttttttt tttttttttt ttgtgttggc tcattagcaa
     5341 gacaaggtga caccattaaa cctccctctg ccattca
//
