LOCUS       NM_003348               4877 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 N (UBE2N), mRNA.
ACCESSION   NM_003348
VERSION     NM_003348.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Zhang,C., Jia,Q., Zhu,L., Hou,J., Wang,X., Li,D., Zhang,J.,
            Zhang,Y., Yang,S., Tu,Z., Yan,X.X., Yang,W., Li,S., Li,X.J. and
            Yin,P.
  TITLE     Suppressing UBE2N ameliorates Alzheimer's disease pathology through
            the clearance of amyloid beta
  JOURNAL   Alzheimers Dement 20 (9), 6287-6304 (2024)
   PUBMED   39015037
  REMARK    GeneRIF: Suppressing UBE2N ameliorates Alzheimer's disease
            pathology through the clearance of amyloid beta.
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Yang,B., Chen,W., Tao,T., Zhang,J., Kong,D., Hao,J., Yu,C., Liao,G.
            and Gong,H.
  TITLE     UBE2N promotes cell viability and glycolysis by promoting Axin1
            ubiquitination in prostate cancer cells
  JOURNAL   Biol Direct 19 (1), 35 (2024)
   PUBMED   38715121
  REMARK    GeneRIF: UBE2N promotes cell viability and glycolysis by promoting
            Axin1 ubiquitination in prostate cancer cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4877)
  AUTHORS   Deng,H., Jia,S., Tang,J., Rong,F., Xu,C., Chen,X., Wang,Z., Zhu,C.,
            Sun,X., Liao,Q., Liu,W., Li,W., Xiao,W. and Liu,X.
  TITLE     SET7 methylates the deubiquitinase OTUB1 at Lys 122 to impair its
            binding to E2 enzyme UBC13 and relieve its suppressive role on
            ferroptosis
  JOURNAL   J Biol Chem 299 (4), 103054 (2023)
   PUBMED   36822329
  REMARK    GeneRIF: SET7 methylates the deubiquitinase OTUB1 at Lys [122] to
            impair its binding to E2 enzyme UBC13 and relieve its suppressive
            role on ferroptosis.
REFERENCE   4  (bases 1 to 4877)
  AUTHORS   Seo,S.U., Woo,S.M., Kim,M.W., Lee,E.W., Min,K.J. and Kwon,T.K.
  TITLE     Phosphorylation of OTUB1 at Tyr 26 stabilizes the mTORC1 component,
            Raptor
  JOURNAL   Cell Death Differ 30 (1), 82-93 (2023)
   PUBMED   35927303
REFERENCE   5  (bases 1 to 4877)
  AUTHORS   Luptak,J., Mallery,D.L., Jahun,A.S., Albecka,A., Clift,D.,
            Ather,O., Slodkowicz,G., Goodfellow,I. and James,L.C.
  TITLE     TRIM7 Restricts Coxsackievirus and Norovirus Infection by Detecting
            the C-Terminal Glutamine Generated by 3C Protease Processing
  JOURNAL   Viruses 14 (8), 1610 (2022)
   PUBMED   35893676
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4877)
  AUTHORS   Moraes,T.F., Edwards,R.A., McKenna,S., Pastushok,L., Xiao,W.,
            Glover,J.N. and Ellison,M.J.
  TITLE     Crystal structure of the human ubiquitin conjugating enzyme
            complex, hMms2-hUbc13
  JOURNAL   Nat Struct Biol 8 (8), 669-673 (2001)
   PUBMED   11473255
REFERENCE   7  (bases 1 to 4877)
  AUTHORS   Chan,N.L. and Hill,C.P.
  TITLE     Defining polyubiquitin chain topology
  JOURNAL   Nat Struct Biol 8 (8), 650-652 (2001)
   PUBMED   11473244
REFERENCE   8  (bases 1 to 4877)
  AUTHORS   Deng,L., Wang,C., Spencer,E., Yang,L., Braun,A., You,J.,
            Slaughter,C., Pickart,C. and Chen,Z.J.
  TITLE     Activation of the IkappaB kinase complex by TRAF6 requires a
            dimeric ubiquitin-conjugating enzyme complex and a unique
            polyubiquitin chain
  JOURNAL   Cell 103 (2), 351-361 (2000)
   PUBMED   11057907
REFERENCE   9  (bases 1 to 4877)
  AUTHORS   Hofmann,R.M. and Pickart,C.M.
  TITLE     Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in
            assembly of novel polyubiquitin chains for DNA repair
  JOURNAL   Cell 96 (5), 645-653 (1999)
   PUBMED   10089880
REFERENCE   10 (bases 1 to 4877)
  AUTHORS   Yamaguchi,T., Kim,N.S., Sekine,S., Seino,H., Osaka,F., Yamao,F. and
            Kato,S.
  TITLE     Cloning and expression of cDNA encoding a human
            ubiquitin-conjugating enzyme similar to the Drosophila bendless
            gene product
  JOURNAL   J Biochem 120 (3), 494-497 (1996)
   PUBMED   8902611
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC000396.2, AK098233.1,
            CB243041.1 and AC025260.29.
            
            On Nov 22, 2018 this sequence version replaced NM_003348.3.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E2
            ubiquitin-conjugating enzyme family. Studies in mouse suggest that
            this protein plays a role in DNA postreplication repair. [provided
            by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.48928.1,
                                           SRR1803615.159199.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000318066.7/ ENSP00000316176.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1204              BC000396.2         30-1233
            1205-2190           AK098233.1         1944-2929
            2191-2249           CB243041.1         17-75               c
            2250-4877           AC025260.29        153460-156087
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q22"
     gene            1..4877
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /note="ubiquitin conjugating enzyme E2 N"
                     /db_xref="GeneID:7334"
                     /db_xref="HGNC:HGNC:12492"
                     /db_xref="MIM:603679"
     exon            1..93
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..522
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /EC_number="2.3.2.23"
                     /note="ubiquitin-conjugating enzyme E2N (homologous to
                     yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13
                     homolog, yeast); bendless-like ubiquitin conjugating
                     enzyme; yeast UBC13 homolog; ubiquitin-protein ligase N;
                     ubiquitin carrier protein N; ubiquitin conjugating enzyme
                     E2N; epididymis secretory protein Li 71; E2
                     ubiquitin-conjugating enzyme N"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 N"
                     /protein_id="NP_003339.1"
                     /db_xref="CCDS:CCDS31875.1"
                     /db_xref="GeneID:7334"
                     /db_xref="HGNC:HGNC:12492"
                     /db_xref="MIM:603679"
                     /translation="MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQD
                     SPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIR
                     TVLLSIQALLSAPNPDDPLANDVAEQWKTNEAQAIETARAWTRLYAMNNI"
     exon            94..340
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /inference="alignment:Splign:2.1.0"
     exon            341..481
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /inference="alignment:Splign:2.1.0"
     exon            482..4877
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1181..1186
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /note="hexamer: AGTAAA"
     polyA_site      1204
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /note="major polyA site"
     regulatory      2169..2174
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /note="hexamer: ATTAAA"
     regulatory      2175..2180
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /note="hexamer: ATTACA"
     polyA_site      2191
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
     polyA_site      2196
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
     regulatory      4850..4855
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
                     /note="hexamer: AATAAA"
     polyA_site      4877
                     /gene="UBE2N"
                     /gene_synonym="HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN"
ORIGIN      
        1 actcgtgcgt gaggcgagag gagccggaga cgagaccaga ggccgaactc gggttctgac
       61 aagatggccg ggctgccccg caggatcatc aaggaaaccc agcgtttgct ggcagaacca
      121 gttcctggca tcaaagccga accagatgag agcaacgccc gttattttca tgtggtcatt
      181 gctggccctc aggattcccc ctttgaggga gggactttta aacttgaact attccttcca
      241 gaagaatacc caatggcagc ccctaaagta cgtttcatga ccaaaattta tcatcctaat
      301 gtagacaagt tgggaagaat atgtttagat attttgaaag ataagtggtc cccagcactg
      361 cagatccgca cagttctgct atcgatccag gccttgttaa gtgctcccaa tccagatgat
      421 ccattagcaa atgatgtagc ggagcagtgg aagaccaacg aagcccaagc catagaaaca
      481 gctagagcat ggactaggct atatgccatg aataatattt aaattgatac gatcatcaag
      541 tgtgcatcac ttctcctgtt ctgccaagac ttcctcctct ttgtttgcat ttaatggaca
      601 cagtcttaga aacattacag aataaaaaag cccagacatc ttcagtcctt tggtgattaa
      661 atgcacatta gcaaatctat gtcttgtcct gattcactgt cataaagcat gagcagaggc
      721 tagaagtatc atctggattg ttgtgaaacg tttaaaagca gtggcccctc cctgctttta
      781 ttcatttccc ccatcctggt ttaagtataa agcactgtga atgaaggtag ttgtcaggtt
      841 agctgcaggg gtgtgggtgt ttttatttta ttttatttta ttttattttt gaggggggag
      901 gtagtttaat tttatgggct cctttccccc ttttttggtg atctaattgc attggttaaa
      961 agcagctaac caggtcttta gaatatgctc tagccaagtc taactttatt tagacgctgt
     1021 agatggacaa gcttgattgt tggaaccaaa atgggaacat taaacaaaca tcacagccct
     1081 cactaataac attgctgtca agtgtagatt ccccccttca aaaaaagctt gtgaccattt
     1141 tgtatggctt gtctggaaac ttctgtaaat cttatgtttt agtaaaatat tttttgttat
     1201 tctactttgc ctttgtacag tttattttac tgtgtttatt tcattttccc aatttgacaa
     1261 tcgtatttta aaattgaaac tgatggaaca ttctttcttg gtcttcacca tctgacaaat
     1321 tgaatggcaa gaggtggatt ttgccagttt cttttcactg atgcagattt gtgttaagat
     1381 agtactgaat ggagtattta taaactggcc ctgagcatgc ataaagcatc agtatctgac
     1441 ctttttttaa ccttctagga atttgaaata aatgtgtttg tgttgtctga ttagatgatc
     1501 attggtgtct tgccacaatg tttaaaaatt actgtacagg aaagtcacag caaagatagc
     1561 agttgtgact gacatgtagg actttcacag ttgtgccaca tttttgccta aaatttgggt
     1621 tatgacattt ttcttggttc ttatctgaaa atttcatctg taacctttca tgtgtgttaa
     1681 gaaacactga tctgatcatt tgggatttgc tgaggcattt gtgagtcttc cttataaacc
     1741 tgatgagcag atctcaacta tctagcttgt gtgtcatcag aaaggtttat ccctttgaga
     1801 gtatcaagtc ctcagttaat gattcttgct ttcatccctc cagtatttgc tgtgggagct
     1861 cgttttattc tttaatttgg aattcagtaa tttttcttct ttattgacga attcctcccc
     1921 tcacaaaact gttctttccc acctctctcc atatctaatt cctgattctt gttattttta
     1981 agtcataaat gtagccagtc ataaatacat aaatgttaac cttcgggttg caaccttgtc
     2041 tcttgcagtt taaggtaatg gatattgtag cccatttgaa ttttcttcac tcttattctc
     2101 gtaattctgg agtttcttca gattgtggtg tattttattg tgctcctatg taagatgaag
     2161 aattaactat taaaattaca ttttcaacat acaaaagctt ttgatgactg gtaactggta
     2221 tccttccaaa taaatgcatt gcttggtaac aaatgttagc ttaattcaga atgaaatagt
     2281 taacaaccct aatattgtaa aatttgaaaa ctgatagcag ctgtcattta taacaattct
     2341 tgggctgttt aaagggtaca tttgactata gctaaggaaa ttgaatctca ctttaaaaat
     2401 gaaccacaca aaacttcaag aaacgaaata gaaatcaaca tttactgccc gtaatattgc
     2461 tcatggttat agctagtatc acatagtgat ttacaaacca tgaaattcca gatttaaatc
     2521 tgttgcacta caaaaatgtc taaatgatgt ctgctaaatt tgtgagttat agaagtacag
     2581 aaataagtca ttttcatttc aaatgcagtg aatttctaat ttcatggtat catatttttt
     2641 tcctttgcag ttttggtaag actcccccat tactttgcta agacttgctt tctcaaccag
     2701 tgggatgaaa cgagtgccag tgtgttgact gagccttctt aggacttaag gggctctggg
     2761 agaggccaag gcagcagttg aaaactgcag cttcacctgg cctttgggcc tgttttccct
     2821 ggcccacaca gtgtggctgg ttaaaacatt ctgaatttga tgccaacatt tttaaaaatt
     2881 gggagatttg catgaaaatc cagaattcgg gtaacttttg aatgatctga agatctggca
     2941 acagtagacc tgacccacct ttgtgcacgg gacttaactg agaggatctg agtagcagac
     3001 accctaagag catgtcattg tcttctcacc agttactctc cagttggcta gagtcccctc
     3061 cgagcacacc tgctagtcct tcccccttgt aagcctttga ctttgtaatc cctgggctaa
     3121 aggaaacaca ttattcaaca aaagtgtttc atgtttgccc actagttgtt ggggatgatt
     3181 aggtggtgag tgaatggcat ggggctttcc tttggggacc atatactcta atggggaagg
     3241 gggacagaca aatagttgta actctgtcat ggaaaagaga agtaacttgg acagactttt
     3301 ggattcaggc cagatgaaca tctccaggca gagggaacag cagcatctgc aaagtcctgg
     3361 gagagaagaa aagaatctga acattaagtg gcttcctgaa aatcgactgc ccaggccggg
     3421 cacagtggct catgcctgta atcccagcac tttaggaggc caaggcagga ggatcacttg
     3481 agtccaggag tttgtgacca gcctggctaa catagtggga cccagtcttt atttaaaaga
     3541 aaattgcccg ttaagaggct tttctaaaat cttgaggtgt tgataggagc tttatgtttt
     3601 ttgacttgat tgttgtagtc tacttcgctg cgatttgctt ctgctctagt tatccttcat
     3661 cctctcaaag gctgtctagc aagatcttag gtctaaagta aagtcatctc ttggtatttg
     3721 tcgggaactg gttctaggac ttcctttgga tatccaaatt cgcagatgct caagtctctg
     3781 gaataaaatg atgttgtata tgcatacaat ctacacaaat cctcccgctt atgttaatat
     3841 ttagattact tataatacct attataatgt aagtgctatg taaatagtta cactattgtt
     3901 taaggaataa tgacaaaaat atacatgttg actacagaca caatgttttt tggaatattt
     3961 tgtctggttg tttgaatcca cggaagtgga acccatggat atggagggcc aactatatag
     4021 aaccttactc tagaccaaga atgtacttgt aaaacaattt caaagaagca cttaaaaata
     4081 agattaaata caaaccaaca gataaaagtc acaggtgcta tggttgtttg atggagaatg
     4141 gggaaggaaa tctgtcctaa aagacattat aaacacagtg acctaagtgg aactaaattg
     4201 gatcgaggaa atgataggaa taggcctcat ttttctgttg aatggttcag gaaccttttt
     4261 tttttttttt tttttttttt tttttggctg ctctagccac tttctagctc tgtagtttag
     4321 gctggagtgc tgtggcatga tcttggctca ctgcaggttc tgcctcccgc gttcaagcga
     4381 ttctcctgcc tcagcctcct gaggagctgg gactacagac acctgccacc acacctggct
     4441 aattttttgt atttttagta gagacggggt ttcaccatgt tgggccagga tggtctccat
     4501 ctcctgacct cgtgatctgc ccgcctcggc ctcccaaagt gctgggatta caggcgtgag
     4561 ccaccgtgcc cagccctgcc acatcttttt tgttagttgc agtagcaata caaccttatc
     4621 tagaaaagct ggccaaaaaa gaaaatacca tgataatagg gtacctaatt tattgcttta
     4681 tttaagaaaa atgtaatatt tcattcgaat aagtactttt gggctgggat gatatttcat
     4741 ttagctactt ggcatgccac ccatcttagg gaattatatg agggtttata ctttaaataa
     4801 cataaaaagc aaaacatgct gaatgtaata atttgagaga agtgtaaata ataaaaatct
     4861 gtcatctacc acctgga
//
