LOCUS       NM_003969               1159 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.
ACCESSION   NM_003969
VERSION     NM_003969.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Lin,X., Sun,D., Yang,S., Cheng,K., Wang,X., Meng,W., Wu,H., Liu,W.,
            Wu,X., Yang,H., Wang,X. and Zhou,L.
  TITLE     UBE2M forms a positive feedback loop with estrogen receptor to
            drive breast cancer progression and drug resistance
  JOURNAL   Cell Death Dis 15 (8), 590 (2024)
   PUBMED   39138151
  REMARK    GeneRIF: UBE2M forms a positive feedback loop with estrogen
            receptor to drive breast cancer progression and drug resistance.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Ma,Y., Huang,X., Wang,Y., Lei,Y., Yu,J., Yu,S., Gao,Y., Yang,J.,
            Zhao,F., Yu,H., Zeng,J., Chu,Y., Yang,M., Li,G., Xie,X. and
            Zhang,J.
  TITLE     NNMT/1-MNA Promote Cell-Cycle Progression of Breast Cancer by
            Targeting UBC12/Cullin-1-Mediated Degradation of P27 Proteins
  JOURNAL   Adv Sci (Weinh) 11 (9), e2305907 (2024)
   PUBMED   38126621
  REMARK    GeneRIF: NNMT/1-MNA Promote Cell-Cycle Progression of Breast Cancer
            by Targeting UBC12/Cullin-1-Mediated Degradation of P27 Proteins.
REFERENCE   3  (bases 1 to 1159)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 1159)
  AUTHORS   Zhao,X., Jiang,L., Hu,D., Tang,Y., Zhao,G., Du,X., Luo,S. and
            Tang,W.
  TITLE     NPRL2 reduces the niraparib sensitivity of castration-resistant
            prostate cancer via interacting with UBE2M and enhancing
            neddylation
  JOURNAL   Exp Cell Res 403 (2), 112614 (2021)
   PUBMED   33905671
  REMARK    GeneRIF: NPRL2 reduces the niraparib sensitivity of
            castration-resistant prostate cancer via interacting with UBE2M and
            enhancing neddylation.
REFERENCE   5  (bases 1 to 1159)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 1159)
  AUTHORS   Tomsig,J.L., Snyder,S.L. and Creutz,C.E.
  TITLE     Identification of targets for calcium signaling through the copine
            family of proteins. Characterization of a coiled-coil
            copine-binding motif
  JOURNAL   J Biol Chem 278 (12), 10048-10054 (2003)
   PUBMED   12522145
REFERENCE   7  (bases 1 to 1159)
  AUTHORS   Wada,H., Yeh,E.T. and Kamitani,T.
  TITLE     A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits
            NEDD8 conjugation in vivo
  JOURNAL   J Biol Chem 275 (22), 17008-17015 (2000)
   PUBMED   10828074
REFERENCE   8  (bases 1 to 1159)
  AUTHORS   Chen,Y., McPhie,D.L., Hirschberg,J. and Neve,R.L.
  TITLE     The amyloid precursor protein-binding protein APP-BP1 drives the
            cell cycle through the S-M checkpoint and causes apoptosis in
            neurons
  JOURNAL   J Biol Chem 275 (12), 8929-8935 (2000)
   PUBMED   10722740
REFERENCE   9  (bases 1 to 1159)
  AUTHORS   Gong,L. and Yeh,E.T.
  TITLE     Identification of the activating and conjugating enzymes of the
            NEDD8 conjugation pathway
  JOURNAL   J Biol Chem 274 (17), 12036-12042 (1999)
   PUBMED   10207026
REFERENCE   10 (bases 1 to 1159)
  AUTHORS   Osaka,F., Kawasaki,H., Aida,N., Saeki,M., Chiba,T., Kawashima,S.,
            Tanaka,K. and Kato,S.
  TITLE     A new NEDD8-ligating system for cullin-4A
  JOURNAL   Genes Dev 12 (15), 2263-2268 (1998)
   PUBMED   9694792
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016630.6 and BC058924.1.
            
            On May 17, 2019 this sequence version replaced NM_003969.3.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E2
            ubiquitin-conjugating enzyme family. The encoded protein is linked
            with a ubiquitin-like protein, NEDD8, which can be conjugated to
            cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.204094.1,
                                           SRR1163658.422502.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142670, SAMEA2142853
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000253023.8/ ENSP00000253023.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-339               AC016630.6         6377-6715
            340-434             AC016630.6         7831-7925
            435-473             AC016630.6         8058-8096
            474-577             AC016630.6         8198-8301
            578-641             AC016630.6         8609-8672
            642-1046            AC016630.6         8759-9163
            1047-1159           BC058924.1         925-1037
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.43"
     gene            1..1159
                     /gene="UBE2M"
                     /gene_synonym="hUbc12; UBC-RS2; UBC12"
                     /note="ubiquitin conjugating enzyme E2 M"
                     /db_xref="GeneID:9040"
                     /db_xref="HGNC:HGNC:12491"
                     /db_xref="MIM:603173"
     exon            1..339
                     /gene="UBE2M"
                     /gene_synonym="hUbc12; UBC-RS2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     CDS             231..782
                     /gene="UBE2M"
                     /gene_synonym="hUbc12; UBC-RS2; UBC12"
                     /EC_number="2.3.2.34"
                     /note="ubiquitin-conjugating enzyme E2M (UBC12 homolog,
                     yeast); ubiquitin conjugating enzyme E2M; ubiquitin
                     carrier protein M; yeast UBC12 homolog; ubiquitin-protein
                     ligase M; NEDD8 protein ligase; NEDD8 carrier protein;
                     epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="NEDD8-conjugating enzyme Ubc12"
                     /protein_id="NP_003960.1"
                     /db_xref="CCDS:CCDS12987.1"
                     /db_xref="GeneID:9040"
                     /db_xref="HGNC:HGNC:12491"
                     /db_xref="MIM:603173"
                     /translation="MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLP
                     KTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHP
                     NIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLF
                     EQNVQRSMRGGYIGSTYFERCLK"
     exon            340..434
                     /gene="UBE2M"
                     /gene_synonym="hUbc12; UBC-RS2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     exon            435..473
                     /gene="UBE2M"
                     /gene_synonym="hUbc12; UBC-RS2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     exon            474..577
                     /gene="UBE2M"
                     /gene_synonym="hUbc12; UBC-RS2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     exon            578..641
                     /gene="UBE2M"
                     /gene_synonym="hUbc12; UBC-RS2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     exon            642..1159
                     /gene="UBE2M"
                     /gene_synonym="hUbc12; UBC-RS2; UBC12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1138..1143
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2M"
                     /gene_synonym="hUbc12; UBC-RS2; UBC12"
                     /note="hexamer: ATTAAA"
     polyA_site      1159
                     /gene="UBE2M"
                     /gene_synonym="hUbc12; UBC-RS2; UBC12"
                     /note="major polyA site"
ORIGIN      
        1 gagtccgagg cgctgggcca ggccggagcc ggactacggg agccgaggcg ggccgcgcgg
       61 tgggcgcgga gaggagcgga gcggcgcggc aggccgggcg ggtggcggca gcagcggagg
      121 aggccgcagc tgcgggtccg aggagcggag gcgacgcggg cggcggcggg gggccgggtg
      181 gccggggtcc cgggccccgc ggcggcggca gcggcggcgg cggcggcagg atgatcaagc
      241 tgttctcgct gaagcagcag aagaaggagg aggagtcggc gggcggcacc aagggcagca
      301 gcaagaaggc gtcggcggcg cagctgcgga tccagaagga cataaacgag ctgaacctgc
      361 ccaagacgtg tgatatcagc ttctcagatc cagacgacct cctcaacttc aagctggtca
      421 tctgtcctga tgagggcttc tacaagagtg ggaagtttgt gttcagtttt aaggtgggcc
      481 agggttaccc gcatgatccc cccaaggtga agtgtgagac aatggtctat caccccaaca
      541 ttgacctcga gggcaacgtc tgcctcaaca tcctcagaga ggactggaag ccagtcctta
      601 cgataaactc cataatttat ggcctgcagt atctcttctt ggagcccaac cccgaggacc
      661 cactgaacaa ggaggccgca gaggtcctgc agaacaaccg gcggctgttt gagcagaacg
      721 tgcagcgctc catgcggggt ggctacatcg gctccaccta ctttgagcgc tgcctgaaat
      781 agggttggcg catacccacc cccgccacgg ccacaagccc tggcatcccc tgcaaatatt
      841 tattgggggc catgggtagg ggtttggggg gcggccggtg ggggaatccc ctgccttggc
      901 cttgcctccc cttcctgcca cgtgccccta gttatttttt tttttttaac accatgtgat
      961 taaggtcggc gctgcctccc ccgacccact cagcgatggg aaatgaattg gcttgtctag
     1021 cccccctgct gggtgcttgt tcagccccca ctctgggctg tggagtgggt gggcaacggg
     1081 cctgggtagc tgggcccagg caacccaccc ctccacctct ggaggtccca ccaggctatt
     1141 aaaggggaat gttactgca
//
