LOCUS       NM_003347               2861 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 L3 (UBE2L3),
            transcript variant 1, mRNA.
ACCESSION   NM_003347
VERSION     NM_003347.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Wang,X. and Zhu,Z.
  TITLE     Role of Ubiquitin-conjugating enzyme E2 (UBE2) in two
            immune-mediated inflammatory skin diseases: a mendelian
            randomization analysis
  JOURNAL   Arch Dermatol Res 316 (6), 249 (2024)
   PUBMED   38795139
  REMARK    GeneRIF: Role of Ubiquitin-conjugating enzyme E2 (UBE2) in two
            immune-mediated inflammatory skin diseases: a mendelian
            randomization analysis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Zhang,X., Wei,Y., Wu,F., Li,M., Han,C., Huo,C., Li,Z., Tang,F.,
            He,W., Zhao,Y. and Li,Y.
  TITLE     UBE2L3 expression in human gastric cancer and its clinical
            significance
  JOURNAL   J Cancer Res Clin Oncol 150 (4), 210 (2024)
   PUBMED   38656363
  REMARK    GeneRIF: UBE2L3 expression in human gastric cancer and its clinical
            significance.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2861)
  AUTHORS   Weinberg,J., Whitcomb,E., Bohm,A., Chekkilla,U.K. and Taylor,A.
  TITLE     The E3 ligase SMURF1 stabilizes p27 via UbcH7 catalyzed K29-linked
            ubiquitin chains to promote cell migration SMURF1-UbcH7 K29
            ubiquitination of p27 and cell migration
  JOURNAL   J Biol Chem 300 (3), 105693 (2024)
   PUBMED   38301893
  REMARK    GeneRIF: The E3 ligase SMURF1 stabilizes p27 via UbcH7 catalyzed
            K29-linked ubiquitin chains to promote cell migration SMURF1-UbcH7
            K29 ubiquitination of p27 and cell migration.
REFERENCE   4  (bases 1 to 2861)
  AUTHORS   Khalil,M.I., Yang,C., Vu,L., Chadha,S., Nabors,H., James,C.D.,
            Morgan,I.M. and Pyeon,D.
  TITLE     The membrane-associated ubiquitin ligase MARCHF8 stabilizes the
            human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in
            head and neck cancer
  JOURNAL   J Virol 98 (2), e0172623 (2024)
   PUBMED   38226814
  REMARK    GeneRIF: The membrane-associated ubiquitin ligase MARCHF8
            stabilizes the human papillomavirus oncoprotein E7 by degrading
            CUL1 and UBE2L3 in head and neck cancer.
REFERENCE   5  (bases 1 to 2861)
  AUTHORS   Mauro,D., Manou-Stathopoulou,S., Rivellese,F., Sciacca,E.,
            Goldmann,K., Tsang,V., Lucey-Clayton,I., Pagani,S., Alam,F.,
            Pyne,D., Rajakariar,R., Gordon,P.A., Whiteford,J., Bombardieri,M.,
            Pitzalis,C. and Lewis,M.J.
  TITLE     UBE2L3 regulates TLR7-induced B cell autoreactivity in Systemic
            Lupus Erythematosus
  JOURNAL   J Autoimmun 136, 103023 (2023)
   PUBMED   37001433
  REMARK    GeneRIF: UBE2L3 regulates TLR7-induced B cell autoreactivity in
            Systemic Lupus Erythematosus.
REFERENCE   6  (bases 1 to 2861)
  AUTHORS   Moynihan,T.P., Cole,C.G., Dunham,I., O'Neil,L., Markham,A.F. and
            Robinson,P.A.
  TITLE     Fine-mapping, genomic organization, and transcript analysis of the
            human ubiquitin-conjugating enzyme gene UBE2L3
  JOURNAL   Genomics 51 (1), 124-127 (1998)
   PUBMED   9693040
REFERENCE   7  (bases 1 to 2861)
  AUTHORS   Moynihan,T.P., Ardley,H.C., Leek,J.P., Thompson,J., Brindle,N.S.,
            Markham,A.F. and Robinson,P.A.
  TITLE     Characterization of a human ubiquitin-conjugating enzyme gene
            UBE2L3
  JOURNAL   Mamm Genome 7 (7), 520-525 (1996)
   PUBMED   8672131
REFERENCE   8  (bases 1 to 2861)
  AUTHORS   Nuber,U., Schwarz,S., Kaiser,P., Schneider,R. and Scheffner,M.
  TITLE     Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7
            (E2-F1) and characterization of their interaction with E6-AP and
            RSP5
  JOURNAL   J Biol Chem 271 (5), 2795-2800 (1996)
   PUBMED   8576257
REFERENCE   9  (bases 1 to 2861)
  AUTHORS   Robinson,P.A., Leek,J.P., Thompson,J., Carr,I.M., Bailey,A.,
            Moynihan,T.P., Coletta,P.L., Lench,N.J. and Markham,A.F.
  TITLE     A human ubiquitin conjugating enzyme, L-UBC, maps in the
            Alzheimer's disease locus on chromosome 14q24.3
  JOURNAL   Mamm Genome 6 (10), 725-731 (1995)
   PUBMED   8563171
REFERENCE   10 (bases 1 to 2861)
  AUTHORS   Blumenfeld,N., Gonen,H., Mayer,A., Smith,C.E., Siegel,N.R.,
            Schwartz,A.L. and Ciechanover,A.
  TITLE     Purification and characterization of a novel species of
            ubiquitin-carrier protein, E2, that is involved in degradation of
            non-'N-end rule' protein substrates
  JOURNAL   J Biol Chem 269 (13), 9574-9581 (1994)
   PUBMED   8144544
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU584304.1 and AJ000519.1.
            
            On Jun 2, 2019 this sequence version replaced NM_003347.3.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes (E1s),
            ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases
            (E3s). This gene encodes a member of the E2 ubiquitin-conjugating
            enzyme family. This enzyme is demonstrated to participate in the
            ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in
            vitro. Several alternatively spliced transcript variants have been
            found for this gene. [provided by RefSeq, Sep 2009].
            
            Transcript Variant: This variant (1) uses an alternate 5' splice
            site and downstream translational start site, compared to variant
            4. It encodes a shorter and distinct N-terminus in isoform 1,
            compared to isoform 4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.66788.1, AJ000519.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000342192.9/ ENSP00000344259.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                BU584304.1         47-62
            17-2861             AJ000519.1         1-2845
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.21"
     gene            1..2861
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
                     /note="ubiquitin conjugating enzyme E2 L3"
                     /db_xref="GeneID:7332"
                     /db_xref="HGNC:HGNC:12488"
                     /db_xref="MIM:603721"
     exon            1..58
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..496
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
                     /EC_number="2.3.2.23"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-conjugating enzyme UBCH7; ubiquitin-protein
                     ligase L3; ubiquitin carrier protein L3;
                     ubiquitin-conjugating enzyme E2-F1; E2
                     ubiquitin-conjugating enzyme L3; ubiquitin conjugating
                     enzyme E2L 3"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 L3 isoform 1"
                     /protein_id="NP_003338.1"
                     /db_xref="CCDS:CCDS13790.1"
                     /db_xref="GeneID:7332"
                     /db_xref="HGNC:HGNC:12488"
                     /db_xref="MIM:603721"
                     /translation="MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNP
                     PYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTD
                     QVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD"
     exon            59..154
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
                     /inference="alignment:Splign:2.1.0"
     exon            155..341
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
                     /inference="alignment:Splign:2.1.0"
     exon            342..2861
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1737..1742
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
                     /note="hexamer: AATAAA"
     polyA_site      1768
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
     regulatory      2285..2290
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
                     /note="hexamer: AATAAA"
     polyA_site      2316
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
     regulatory      2839..2844
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
                     /note="hexamer: AATAAA"
     polyA_site      2861
                     /gene="UBE2L3"
                     /gene_synonym="E2-F1; L-UBC; UBCH7; UbcM4"
                     /note="major polyA site"
ORIGIN      
        1 attctgggga aggagcagca ccaaatccaa gatggcggcc agcaggaggc tgatgaagga
       61 gcttgaagaa atccgcaaat gtgggatgaa aaacttccgt aacatccagg ttgatgaagc
      121 taatttattg acttggcaag ggcttattgt tcctgacaac cctccatatg ataagggagc
      181 cttcagaatc gaaatcaact ttccagcaga gtacccattc aaaccaccga agatcacatt
      241 taaaacaaag atctatcacc caaacatcga cgaaaagggg caggtctgtc tgccagtaat
      301 tagtgccgaa aactggaagc cagcaaccaa aaccgaccaa gtaatccagt ccctcatagc
      361 actggtgaat gacccccagc ctgagcaccc gcttcgggct gacctagctg aagaatactc
      421 taaggaccgt aaaaaattct gtaagaatgc tgaagagttt acaaagaaat atggggaaaa
      481 gcgacctgtg gactaaaatc tgccacgatt ggttccagca agtgtgagca gagaccccgt
      541 gcagtgcatt cagacacccc gcaaagcagg actctgtgga aattgacacg tgccaccgcc
      601 tggcgttcgc ttgtggcagt tactaacttt ctacagtttt cttaatcaaa agtggtctag
      661 gtaacctgta aagaaaggat taaaaattta agatgttcta gttctgctct ctttgtttta
      721 aaaatcactg cttcaatcta cttcaaaaga atggtgtttc ttttcttgtc caattttatc
      781 caaaatcttc aagttacatt taacccataa ggtttaaaaa aaaggaaaaa aaacggttgt
      841 ggttcccttt cttccctacc cttgccactc ccactttctg gcaccgagtt tatttttcac
      901 ttacttactt ccccagaccc cgggctcgcc tccacaaagg agaagagact gccctggcgg
      961 tcctggtggc ttttcttagc atgtgtggca ctgttgccca gtgtgggagt tggtttaaat
     1021 tctcctgact ccagtttata acatcctttt aaaaaattta aaaacaaaca gccacacccc
     1081 tcctccagtc cttctcctca gttcttgtgt gaaactccag ctgatgttac cacagtaaca
     1141 tcagttaatt gggcaagccc tgatgtcagt gtgtgtaact gacctctggc ctggcctgca
     1201 cagagaagcc ctataatcac aggtctgtgg tggccccgaa atggggggcc tgctagtcag
     1261 gaggatgctg tgcacactgt gtgtgatgaa tctcgccaga aaggctcctg aggtcccagg
     1321 ttggcacttc tccctgcagc cattgtagaa gatctgctgg tccttgcagg caaagctaca
     1381 gccagaatgt ccgtttgaaa ctcctagctc atctgtcacc gagcttcatc cgaatgtgcc
     1441 acggagcttg ctctccactt cctccgtgca gtggccctgc cacagccctc cctcggcaca
     1501 ctttgaccct ttgtaggatt ggaattagca ggactcggct atttaaagca ccagtctggg
     1561 gtcgcctggg cccctgctga ccccctcctc cagagcagcc agcccagccc gggaacaaga
     1621 cggacttcct ctcccttcgg actcacagcc tttgcagagt caagctccac ttgaagctca
     1681 ctcagtaata tcctttcaat gtgttttata ttgttttgac tgcctttttt tgtagaaata
     1741 aaaattgacc ttagaattta tcgtcagata aacttgtaaa gatttgaata ttaatgtctt
     1801 ttcaaggcaa atgggattgt ccccgcacta gtagagaatc catgtcgctc tgacacccca
     1861 aggaagccga cgatccaaat gccgtgtgtc accaaccccg cttctgccac tggcggcttc
     1921 ccttcttggc tcttgggggg gactagatcc tgtggagaag atgacttaaa ctttgctttt
     1981 tgttttaatt ttaattctat aacttgagat ctttccgggg cctacaggcg tgtaagacag
     2041 cttggtctgg tctgtgcaga agtggggagt gatgggcagg ttcggcagcc taacattgtt
     2101 caggcgcatg gcccctgcgg tgtgtacacg aactcggctt cttttgtcct aggtacgcca
     2161 agggcaggtt tctggagact cccttgtgcc cgggatggca agggcaccgg gctggcgttt
     2221 ccacatctgt cttcattagc agaaaagtga tgatggattt tatttcactc acactccagt
     2281 ttgtaataaa atgccaaatt ctgtcagcta tccaaacaag ccaccatttg ttcttgttgc
     2341 ttctctggat ccagaaatgt tgccattctt ggaaactgtc ccattgcttc gtatttctgc
     2401 caacgtagct ctgcctgcct gtcaacccct cactgcactc tgctcatcac gggaggatac
     2461 ctgtgtgccg gcagcccctc agggactctc agccctggca ctggcacccc agggttggcc
     2521 ccgtcagcag aggcttggct ttcgagccag tgggtgtctc tcctttgggc ctgggcggct
     2581 tgctcctgcc agccatgcct tcagggtagg ctctgagcaa gctggcgaac agccctggct
     2641 gctccaaaac caaaaagctg ggtcctctgg aggaggggcg agctgtggag cagccaccca
     2701 ctgctgcccc aagctcactc aggaattcac acccgcctgg tttcttgaag tgtgctgggt
     2761 ccttccctct gctccctact ccccaccacg gcagagaata ggctttctaa gatgctgcga
     2821 tcccgttctg ctgcccgtaa taaaaatgct ctcagacact g
//
