LOCUS       NM_005339               5153 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 K (UBE2K), transcript
            variant 1, mRNA.
ACCESSION   NM_005339
VERSION     NM_005339.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Xin,Z., Holgersson,K., Zhu,P., Tan,H., Shi,G., Szekely,L. and Wu,T.
  TITLE     Silencing UBE2K inhibits the growth of glioma cells by inducing the
            autophagy-related apoptosis
  JOURNAL   J Biochem Mol Toxicol 38 (7), e23758 (2024)
   PUBMED   38963134
  REMARK    GeneRIF: Silencing UBE2K inhibits the growth of glioma cells by
            inducing the autophagy-related apoptosis.
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Sahu,M., Rani,N. and Kumar,P.
  TITLE     Simulation and Computational Study of RING Domain Mutants of BRCA1
            and Ube2k in AD/PD Pathophysiology
  JOURNAL   Mol Biotechnol 66 (5), 1095-1115 (2024)
   PUBMED   38172369
  REMARK    GeneRIF: Simulation and Computational Study of RING Domain Mutants
            of BRCA1 and Ube2k in AD/PD Pathophysiology.
REFERENCE   3  (bases 1 to 5153)
  AUTHORS   Ouyang,G., Fu,W., Guo,J., Lu,Q., Yao,Y., Ge,L., Zhao,J., Zhang,J.,
            Hu,X., Li,S., Xu,Q., Huang,D. and Zhang,Y.
  TITLE     Hypoxia-induced UBE2K promotes the malignant progression of HCC
  JOURNAL   Pathol Res Pract 245, 154422 (2023)
   PUBMED   37003132
  REMARK    GeneRIF: Hypoxia-induced UBE2K promotes the malignant progression
            of HCC.
REFERENCE   4  (bases 1 to 5153)
  AUTHORS   Lei,X., Hu,X., Lu,Q., Yao,Y., Sun,W., Ma,Q., Huang,D. and Xu,Q.
  TITLE     UBE2K promotes the malignant progression of hepatocellular
            carcinoma by regulating c-Myc
  JOURNAL   Biochem Biophys Res Commun 638, 210-218 (2023)
   PUBMED   36481361
  REMARK    GeneRIF: UBE2K promotes the malignant progression of hepatocellular
            carcinoma by regulating c-Myc.
REFERENCE   5  (bases 1 to 5153)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 5153)
  AUTHORS   Kikuchi,J., Furukawa,Y., Kubo,N., Tokura,A., Hayashi,N.,
            Nakamura,M., Matsuda,M. and Sakurabayashi,I.
  TITLE     Induction of ubiquitin-conjugating enzyme by aggregated low density
            lipoprotein in human macrophages and its implications for
            atherosclerosis
  JOURNAL   Arterioscler Thromb Vasc Biol 20 (1), 128-134 (2000)
   PUBMED   10634809
REFERENCE   7  (bases 1 to 5153)
  AUTHORS   Petersen,A., Mani,K. and Brundin,P.
  TITLE     Recent advances on the pathogenesis of Huntington's disease
  JOURNAL   Exp Neurol 157 (1), 1-18 (1999)
   PUBMED   10222105
  REMARK    Review article
REFERENCE   8  (bases 1 to 5153)
  AUTHORS   Whitby,F.G., Xia,G., Pickart,C.M. and Hill,C.P.
  TITLE     Crystal structure of the human ubiquitin-like protein NEDD8 and
            interactions with ubiquitin pathway enzymes
  JOURNAL   J Biol Chem 273 (52), 34983-34991 (1998)
   PUBMED   9857030
REFERENCE   9  (bases 1 to 5153)
  AUTHORS   Coux,O. and Goldberg,A.L.
  TITLE     Enzymes catalyzing ubiquitination and proteolytic processing of the
            p105 precursor of nuclear factor kappaB1
  JOURNAL   J Biol Chem 273 (15), 8820-8828 (1998)
   PUBMED   9535861
REFERENCE   10 (bases 1 to 5153)
  AUTHORS   Kalchman,M.A., Graham,R.K., Xia,G., Koide,H.B., Hodgson,J.G.,
            Graham,K.C., Goldberg,Y.P., Gietz,R.D., Pickart,C.M. and
            Hayden,M.R.
  TITLE     Huntingtin is ubiquitinated and interacts with a specific
            ubiquitin-conjugating enzyme
  JOURNAL   J Biol Chem 271 (32), 19385-19394 (1996)
   PUBMED   8702625
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA526283.1, BC022804.1,
            AC105287.5 and BU622273.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005339.4.
            
            Summary: The protein encoded by this gene belongs to the
            ubiquitin-conjugating enzyme family. This protein interacts with
            RING finger proteins, and it can ubiquitinate huntingtin, the gene
            product for Huntington's disease. Known functions for this protein
            include a role in aggregate formation of expanded polyglutamine
            proteins and the suppression of apoptosis in polyglutamine
            diseases, a role in the dislocation of newly synthesized MHC class
            I heavy chains from the endoplasmic reticulum, and involvement in
            foam cell formation. Multiple transcript variants encoding
            different isoforms have been identified for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.57829.1,
                                           SRR1660803.204950.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261427.10/ ENSP00000261427.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                DA526283.1         93-124
            33-994              BC022804.1         1-962
            995-4470            AC105287.5         27967-31442
            4471-5153           BU622273.1         15-697              c
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p14"
     gene            1..5153
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /note="ubiquitin conjugating enzyme E2 K"
                     /db_xref="GeneID:3093"
                     /db_xref="HGNC:HGNC:4914"
                     /db_xref="MIM:602846"
     exon            1..255
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             193..795
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /EC_number="2.3.2.23"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin carrier protein; ubiquitin-conjugating enzyme
                     E2K (UBC1 homolog, yeast); ubiquitin-protein ligase;
                     ubiquitin-conjugating enzyme E2-25 KDA; HIP-2; E2(25K);
                     huntingtin-interacting protein 2; ubiquitin-conjugating
                     enzyme E2(25K); E2 ubiquitin-conjugating enzyme K;
                     ubiquitin conjugating enzyme E2K"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 K isoform 1"
                     /protein_id="NP_005330.1"
                     /db_xref="CCDS:CCDS33976.1"
                     /db_xref="GeneID:3093"
                     /db_xref="HGNC:HGNC:4914"
                     /db_xref="MIM:602846"
                     /translation="MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIA
                     GPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAA
                     AMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSP
                     EYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN"
     exon            256..349
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            350..408
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            409..491
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            492..591
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            592..720
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            721..5153
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      969..974
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /note="hexamer: ATTAAA"
     polyA_site      994
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /note="major polyA site"
     polyA_site      4087
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
     regulatory      5124..5129
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
                     /note="hexamer: AATAAA"
     polyA_site      5153
                     /gene="UBE2K"
                     /gene_synonym="E2-25K; HIP2; HYPG; LIG; UBC1"
ORIGIN      
        1 gccattttgg tggccgggcg cggaggtgat tccacactga ggcgagcgcg gcggccgggg
       61 tggtagtggc agtgttcgtg tgctcaggtc tgaatcgccg agggaggagg cggtggagga
      121 agaggtggcg gcggtggcgg tggtcgtagc ggtggcggag gaggcgggta cgaatcagct
      181 gcgggcggag acatggccaa catcgcggtg cagcgaatca agcgggagtt caaggaggtg
      241 ctgaagagcg aggagacgag caaaaatcaa attaaagtag atcttgtaga tgagaatttt
      301 acagaattaa gaggagaaat agcaggacct ccagacacac catatgaagg aggaagatac
      361 caactagaga taaaaatacc agaaacatac ccatttaatc cccctaaggt ccggtttatc
      421 actaaaatat ggcatcctaa tattagttcc gtcacagggg ctatttgttt ggatatcctg
      481 aaagatcaat gggcagctgc aatgactctc cgcacggtat tattgtcatt gcaagcacta
      541 ttggcagctg cagagccaga tgatccacag gatgctgtag tagcaaatca gtacaaacaa
      601 aatcccgaaa tgttcaaaca gacagctcga ctttgggcac atgtgtatgc tggagcacca
      661 gtttctagtc cagaatacac caaaaaaata gaaaacctat gtgctatggg ctttgatagg
      721 aatgcagtaa tagtggcctt gtcttcaaaa tcatgggatg tagagactgc aacagaattg
      781 cttctgagta actgaggcat agagagctgc tgatatagtc aagcttgcct cttcttgagg
      841 agcaccaaca tctgttattt ttaggattct gcatagattt cttttaaact ggcattcttg
      901 cctaatgatg ttatctaggc accattggag actgaaaaaa aaaaatccct gctctgtaaa
      961 taaagctaat taaacgtctg tgtaaattta aaaaggggaa atactttaat tttttttctt
     1021 aatagtgtaa aaattccctg agctaagcta aaaccatgga agaaacatgc tactttagtg
     1081 tttagcagtg taccaagact agcaagagtt tgcttcagga ttttgttgaa taattaagat
     1141 aatattttga gtgtgtcagg gccattcaaa ttgttggtgt tgcatcacag ctaccttaac
     1201 tgtttttaac atggatcctc tgtgcctgtg aatttacttg catgcttgta cttgacttct
     1261 taggatgggt agctgaaaag accaccattt taagcatttg agaattctta aatatgaaat
     1321 ttattcagaa ttgaagatgg tgacctattc agagcctttt tgtccttgtc aacagactgg
     1381 gacagtgtct gattccccct tcaccccccc cacccccgcc ttgccacaca cagctaatat
     1441 tctaatggta aatttctctg tatcaggtgg ggaaatgtgc tgaaggacag tatgtatccc
     1501 ttgcttcatt tttaggtcgt aggtttggaa tgtcttgtcc cagttcttca aacactctta
     1561 aatttttctt aagtaatgta aaaatggaac tgccaatttt atttctcttg caaaaatagt
     1621 aaatacttga tgttacatta ttcccaggtt taatgaaaga acccaactta gtttttcagt
     1681 gaatttgaca cctatttttt agtgatgaaa tttttctttg agaactggca aggatgcagt
     1741 cagctgtttg cagtttttag cctgattttg gggtctatag agattgcttt attggatact
     1801 tcaagtcatt cttgcttgca cttcccctat tgacacatga aagctgtgtt ggtgttttat
     1861 tgtacatact tcagatgcac ataggaatag aagtgtgtta taaatctagc tttctttatg
     1921 atgtttctga taatacgaga attgaaaact ttaccttctc ttgtacatag tcagactatt
     1981 tgtattaaat ttacatttca ttctaagttc aaaagtttga aaattattag ttttgcaaga
     2041 tcacacacta atgtaaccat tttatgaagg ttgaagtgga tttatgcagg cagttctata
     2101 tatagaaata caattctttt taaattttta ggaccaatac aaaataacaa aaatgtaatg
     2161 gaatcagact gaattaaagt aaggctgtat attgaaagtc atattataaa aggtttgctt
     2221 tctttaagtg ttatttatct taaattataa tcgttaaatg tttggaagat aatttttgaa
     2281 tcataacgtc agcataactt catttgactt ctcaataatc ttgatactag aagcaataaa
     2341 agaaccatta ttaaatatat tgtaatgtta aagatgtgaa ggttcttcca aaaatttgta
     2401 aggctttcct aattaacagt aaactcttat agctgatatt attacatact aaacacaagt
     2461 tgctgttttt tccccatatg tttgcttacg catgtcttta tacaatcacc tctttttatt
     2521 gctgtagttt attttctaaa gatgccaaag gaaacaagat ttttctttat ttattttaaa
     2581 tacctgaaac ctcgtacttt atattttgaa gtaaggtggg attgtgtgaa aatgtggatg
     2641 gtttttctcc agtgttggtt agaactactt tttttaagtg gagggcaact tggtagtatt
     2701 agtagaattt gggtttaaaa atatcagaca catctaccca gtttattttt tgcctgtatt
     2761 atcagggtat atttaattgt cctgtggtta aacaaaggtg agagagctca gaggtttcta
     2821 aaggtcaagc attttacata tatacataca tacatgctct tggaggcagc tatatcctgc
     2881 tttgattttt atgcacatga gccctatagg gttatatact catctaacta cacttcagca
     2941 tccccagtgc ccaagaaatt ggaaagtaca atatttggtt tgagacattc ttcataaaag
     3001 attctttatt ataaaaattt acctcattta catttaatgt gatgatcttt tttttccttt
     3061 ctggtatatt tcccactgta ctgtttggtt tttgttattc tcttgtctgt gggtagattt
     3121 agttacccaa acatagaaac taaagcaatt gcttttttcc tctggtctta aatatgtttg
     3181 gacaaaaaga cactaaaagg tcaggcctag gggaaaagta ccctggtgga aattattttt
     3241 gtaattctta atatttttga gttggctccg tttgtcagtt ttaaaagcct caaaaattat
     3301 gttaaaaaaa aaatccagtg aaatcccatt tagggcaggc cgatttgtta gaagtcactg
     3361 atgctccagt aggcttttgt tataatcagg cctgtttcct gagtatgtgc ctcttttaaa
     3421 agaagaaata attagctgta tagtatttca gtactgatga atgaatgaat gaatgaatgg
     3481 cagttagtgg acccaaataa gtattcagtg gtatataaat atttgaggaa ctaggtactg
     3541 aattaggtgc tctgagttgg caccccgaaa tgtttggaat cacgactatg atttacttaa
     3601 acacgtaaca cattcaagga tatataaatg gagtctaagt attttgcttt tgaagagaat
     3661 aatctatgaa atggaagact tgattttttt tttcattttt tagaagtaat acacatttat
     3721 gattgttggc acataaaact atgcaaaata attcttttta agtatctgta agaaagaatt
     3781 ggcagtttaa acggattttg cttatttatg cactacagaa tgcagcatac tgtattactg
     3841 ttgaggctgc ttaataagtt taccaatgtg aaaagcagat cttgggtata agtgtatgcc
     3901 tgatttgagc ttttaaaact gtgcagtagg tgtatggtaa tgttttacaa tcctatgtag
     3961 caaaatagga aattatgaat ttttgttgtt attgttttaa agatttaaga tggattgggg
     4021 tagggaacag ctggagccag gtataccttc tagtatgtag agggaaacaa tgtttagata
     4081 ggaaaaagga agccgtctgt ttacagttaa ctttatttca tggacttcta caaaatgttg
     4141 tattaatcac tttttctagt tcaaggaatt tcaattccag ggtacaaacc attgatttaa
     4201 aaattacgtt tccattcttt ttagtatcag tttaaagtaa atgcttacca tcagccagtt
     4261 gctgtcactg ggaagaaggc aacttgaagt atttactgat aaaatagcct ttattcccaa
     4321 gtgtgacttt tcttcagtgt ctacatatta tgtcacacgt tctatttgca ctgctgcaat
     4381 atagatcaaa gatcttgtgg cataggggta ggggagtgtt agtatctccc ttgtcccatt
     4441 tgttctgtta ctgcttcatt ggactgatac accctcatga acttgcaatc acctaaatgg
     4501 aagtcttgaa tgtgtcacct tttgcttggt tatttcaacc atgctgctat atataatcag
     4561 gtgtggcaca gtttgtcagt ttgattatag gtgtgagtgg atagaaatca ttacactgtg
     4621 ctgttaacga tccttgtctg cttgctgtta cctacttttt acaggcatca taataaaagc
     4681 tagactattt ctttgggggg aaagagactt atttaatgta atttaaaaaa cttttccaat
     4741 agaaaatgaa atattattga aaataatatc tattttttag ctttcagcaa ttgatggtgc
     4801 tttgttgtgg tgtctgctgg aagtctactg ccattatagg gaaccttgct tgttagcttc
     4861 tctagatctc tattctaaac aatctgttag tgatgataaa ttctgtagga gggtctattc
     4921 tgagccgtta acttcctgta aggggaaaat gggtgggtta ccagaaatac cattgaagca
     4981 gggtgggctg tggggtggaa ggttggggta tttgtcttga gaattaaaaa ctacgaaaca
     5041 cttttgtaca caactgattt tttaaaaaat aaacacattt ttaaagatgt tgaatttttc
     5101 cccccttatt gggaattctt aaaaataaat gcatgcatgt tttcccctga aaa
//
