LOCUS       NM_058167               2260 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens ubiquitin conjugating enzyme E2 J2 (UBE2J2),
            transcript variant 2, mRNA.
ACCESSION   NM_058167 NM_018618
VERSION     NM_058167.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Jiang,C., Dai,X., He,S., Zhou,H., Fang,L., Guo,J., Liu,S.,
            Zhang,T., Pan,W., Yu,H., Fu,T., Li,D., Inuzuka,H., Wang,P., Xiao,J.
            and Wei,W.
  TITLE     Ring domains are essential for GATOR2-dependent mTORC1 activation
  JOURNAL   Mol Cell 83 (1), 74-89 (2023)
   PUBMED   36528027
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 2260)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2260)
  AUTHORS   Tan,J.M.E., Cook,E.C.L., van den Berg,M., Scheij,S., Zelcer,N. and
            Loregger,A.
  TITLE     Differential use of E2 ubiquitin conjugating enzymes for regulated
            degradation of the rate-limiting enzymes HMGCR and SQLE in
            cholesterol biosynthesis
  JOURNAL   Atherosclerosis 281, 137-142 (2019)
   PUBMED   30658189
  REMARK    GeneRIF: UBE2J2 as an important partner of MARCH6 for regulated
            degradation of the rate-limiting enzymes HMGCR and SQLE in
            cholesterol biosynthesis.
REFERENCE   5  (bases 1 to 2260)
  AUTHORS   van de Weijer,M.L., Schuren,A.B.C., van den Boomen,D.J.H.,
            Mulder,A., Claas,F.H.J., Lehner,P.J., Lebbink,R.J. and
            Wiertz,E.J.H.J.
  TITLE     Multiple E2 ubiquitin-conjugating enzymes regulate human
            cytomegalovirus US2-mediated immunoreceptor downregulation
  JOURNAL   J Cell Sci 130 (17), 2883-2892 (2017)
   PUBMED   28743740
  REMARK    GeneRIF: UBE2G2 was crucial for the degradation of various
            immunoreceptors. UBE2J2, on the other hand, counteracted
            US2-induced endoplasmic reticulum-associated protein degradation by
            downregulating TRC8 expression.
REFERENCE   6  (bases 1 to 2260)
  AUTHORS   El Khouri,E., Le Pavec,G., Toledano,M.B. and Delaunay-Moisan,A.
  TITLE     RNF185 is a novel E3 ligase of endoplasmic reticulum-associated
            degradation (ERAD) that targets cystic fibrosis transmembrane
            conductance regulator (CFTR)
  JOURNAL   J Biol Chem 288 (43), 31177-31191 (2013)
   PUBMED   24019521
REFERENCE   7  (bases 1 to 2260)
  AUTHORS   David,Y., Ziv,T., Admon,A. and Navon,A.
  TITLE     The E2 ubiquitin-conjugating enzymes direct polyubiquitination to
            preferred lysines
  JOURNAL   J Biol Chem 285 (12), 8595-8604 (2010)
   PUBMED   20061386
  REMARK    GeneRIF: analysis of involvement of E2 enzymes in specifying
            polyubiquitin chain assembly
REFERENCE   8  (bases 1 to 2260)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   9  (bases 1 to 2260)
  AUTHORS   Tiwari,S. and Weissman,A.M.
  TITLE     Endoplasmic reticulum (ER)-associated degradation of T cell
            receptor subunits. Involvement of ER-associated
            ubiquitin-conjugating enzymes (E2s)
  JOURNAL   J Biol Chem 276 (19), 16193-16200 (2001)
   PUBMED   11278356
REFERENCE   10 (bases 1 to 2260)
  AUTHORS   Chen,P., Johnson,P., Sommer,T., Jentsch,S. and Hochstrasser,M.
  TITLE     Multiple ubiquitin-conjugating enzymes participate in the in vivo
            degradation of the yeast MAT alpha 2 repressor
  JOURNAL   Cell 74 (2), 357-369 (1993)
   PUBMED   8393731
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG717336.1, BG499172.1,
            AK056065.1, BQ881730.1, AK098803.1 and BE515187.1.
            
            On Jun 2, 2019 this sequence version replaced NM_058167.2.
            
            Summary: The modification of proteins with ubiquitin is an
            important cellular mechanism for targeting abnormal or short-lived
            proteins for degradation. Ubiquitination involves at least three
            classes of enzymes: ubiquitin-activating enzymes, or E1s,
            ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein
            ligases, or E3s. This gene encodes a member of the E2
            ubiquitin-conjugating enzyme family. This enzyme is located in the
            membrane of the endoplasmic reticulum. Multiple alternatively
            spliced transcript variants have been found for this gene, but the
            full-length nature of some variants has not been defined. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) lacks an in-frame internal
            exon, as compared to variant 1. The encoded isoform (2) is thus
            missing an internal segment, as compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC094777.1, SRR14038193.927756.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000349431.11/ ENSP00000305826.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-138               BG717336.1         6-143
            139-320             BG499172.1         132-313
            321-395             AK056065.1         231-305
            396-1089            BQ881730.1         85-778
            1090-2258           AK098803.1         456-1624
            2259-2260           BE515187.1         345-346
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..2260
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /note="ubiquitin conjugating enzyme E2 J2"
                     /db_xref="GeneID:118424"
                     /db_xref="HGNC:HGNC:19268"
                     /db_xref="MIM:619756"
     exon            1..189
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /inference="alignment:Splign:2.1.0"
     CDS             190..969
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /EC_number="2.3.2.23"
                     /note="isoform 2 is encoded by transcript variant 2;
                     ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast);
                     yeast UBC6 homolog; ubiquitin conjugating enzyme 6;
                     non-canonical ubiquitin-conjugating enzyme 2; E2
                     ubiquitin-conjugating enzyme J2"
                     /codon_start=1
                     /product="ubiquitin-conjugating enzyme E2 J2 isoform 2"
                     /protein_id="NP_477515.2"
                     /db_xref="CCDS:CCDS14.1"
                     /db_xref="GeneID:118424"
                     /db_xref="HGNC:HGNC:19268"
                     /db_xref="MIM:619756"
                     /translation="MSSTSSKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEW
                     HYVVRGPEMTPYEGGYYHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHP
                     DTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKRQLAVQSLAFNLKDKVFCELF
                     PEVVEEIKQKQKAQDELSSRPQTLPLPDVVPDGETHLVQNGIQLLNGHAPGAVPNLAG
                     LQQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE"
     exon            190..320
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /inference="alignment:Splign:2.1.0"
     exon            321..361
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /inference="alignment:Splign:2.1.0"
     exon            362..464
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /inference="alignment:Splign:2.1.0"
     exon            465..603
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /inference="alignment:Splign:2.1.0"
     exon            604..684
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /inference="alignment:Splign:2.1.0"
     exon            685..2260
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1250..1255
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /note="hexamer: AGTAAA"
     polyA_site      1271
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /note="major polyA site"
     regulatory      2241..2246
                     /regulatory_class="polyA_signal_sequence"
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /note="hexamer: ATTATA"
     polyA_site      2260
                     /gene="UBE2J2"
                     /gene_synonym="NCUBE-2; NCUBE2; PRO2121; UBC6"
                     /note="major polyA site"
ORIGIN      
        1 ggttccgccc cgcgagcggc catcttggag gctgaggcgg cggcggcggc gctgcggcgg
       61 gttcggtggg cccaatcccg gggcggtgcg gctgtttcgg gcgcgggccc cgcttttccg
      121 caccctgctc cggcctcgac tacggcgagc ctgagcgcgg cggcggccca cgcgcagcga
      181 cagggagaga tgagcagcac cagcagtaag agggctccga ccacggcaac ccagaggctg
      241 aagcaggact accttcgcat taagaaagac ccggtgcctt acatctgtgc cgagcccctc
      301 ccttcgaata ttctcgagtg gcactatgtc gtccgaggcc cagagatgac cccttatgaa
      361 ggtggctatt atcatggaaa actaattttt cccagagaat ttcctttcaa acctcccagt
      421 atctatatga tcactcccaa cgggaggttt aagtgcaaca ccaggctgtg tctttctatc
      481 acggatttcc acccggacac gtggaacccg gcctggtctg tctccaccat cctgactggg
      541 ctcctgagct tcatggtgga gaagggcccc accctgggca gtatagagac gtcggacttc
      601 acgaaaagac aactggcagt gcagagttta gcatttaatt tgaaagataa agtcttttgt
      661 gaattatttc ctgaagtcgt ggaggagatt aaacaaaaac agaaagcaca agacgaactc
      721 agtagcagac cccagactct ccccttgcca gacgtggttc cagacgggga gacgcacctc
      781 gtccagaacg ggattcagct gctcaacggg catgcgccgg gggccgtccc aaacctcgca
      841 gggctccagc aggccaaccg gcaccacgga ctcctgggtg gcgccctggc gaacttgttt
      901 gtgatagttg ggtttgcagc ctttgcttac acggtcaagt acgtgctgag gagcatcgcg
      961 caggagtgag gcccaggcgc cgagacccaa ggcgccactg agggcaccgc gcaccagagc
     1021 gtgacctcgg caggctggac acactgccca gcacaggcag acccaccagg ctcctaggtt
     1081 tagcttttaa aaacctgaaa ggggaagcaa aaaccaaaat gtgtgactgg gctttggagg
     1141 agactggagc ctcagccctg tcctggccac gggccgctgg ggctggtgtg ggtgggcctt
     1201 gtgtgctgga tttgtagctt atcttccgtg ttgtctttgg acctgtttta gtaaacccgt
     1261 ttttcatttt attagatgtg gtcacttaga aatgcaaact tgctgccgac cgcgggctgc
     1321 tcctgcgttc ttggagctcc tggcgcgttt ctcggagctc ccggctcctc agcgggtggg
     1381 aacctcgggg cccaggggtg gagctggcgt ccgcgggtgc tggtctggcc tggccgtgtg
     1441 gtgatgaggc ttagcggggc cagtgacggc cgtggctcag gatccataag tcggggtttg
     1501 gtctcagcat ttacaaatgt gtttacagtc agaatgaaac acattccttc tagaaagtgc
     1561 ttgggggttt ttgctgccct ggaagccagg agcctgctca ctccaaccac aagtcgccct
     1621 tgactgcggc ggccgcgagc ggggcggggg ctgccggtgc cctccgcagg ccgggcctcc
     1681 tgggcgcccc tcggtgctgc aggctggggg gccttgggta cctgcagagc cttttctctg
     1741 aattccttat gtccggtggg ccagaagccc gtcctcctat gctggtggaa ggcggaggac
     1801 cggagtccct gcagaaggcc ccgtgcactc gggggcctcc ctcacatccc gtgccccctg
     1861 cgctggcctt cacagtaggt aatggctccg gcccgggtgt tcgctgtcca cggaacatgg
     1921 cagaggggca ccccggcccg gaaagacgcc agagccagca ggggctgttt cgggccgcgt
     1981 ggctccccgg gtctcggccg tctcccctct tctgcgtctg ttccgtgact tcgcctgggt
     2041 gggatgtacc gcaggtgcat cgcgtcgagg tggggcacgg ccgccggcaa gaaacccacc
     2101 ctgtccggag gcgggcgtga gacaagccca gcccgcacgc gctcatcttt cttcgttttt
     2161 tgatcagttt attcagaatt gctctataat ttaccaattg tatgtattta acctattctt
     2221 gtggaaaaaa aaggtctttc attatatctt tatttctgaa
//
